scholarly journals Effects of DNA topoisomerase IIα splice variants on acquired drug resistance

2020 ◽  
Author(s):  
Terry S. Elton ◽  
Hatice Gulcin Ozer ◽  
Jack C. Yalowich
Keyword(s):  
Drug Resistance ◽  
Splice Variants ◽  
Topoisomerase Iiα ◽  
Dna Topoisomerase ◽  
Acquired Drug Resistance ◽  
Dna Topoisomerase Iiα

scholarly journals DNA Topoisomerase IIα in Multiple Myeloma: A Marker of Cell Proliferation and Not Drug Resistance

2001 ◽  
Vol 14 (9) ◽  
pp. 886-891 ◽  
Author(s):  
Carla S. Wilson ◽  
L. Jeffrey Medeiros ◽  
Raymond Lai ◽  
Anthony W. Butch ◽  
Althea McCourty ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Cell Proliferation ◽  
Drug Resistance ◽  
Topoisomerase Iiα ◽  
Dna Topoisomerase ◽  
Dna Topoisomerase Iiα

scholarly journals Sp1 mediates cell proliferation-dependent regulation of rat DNA topoisomerase IIα gene promoter

1999 ◽  
Vol 344 (2) ◽  
pp. 367-374 ◽  
Author(s):  
Jeong Ho YOON ◽  
Jeong Kee KIM ◽  
Geun Bae RHA ◽  
Misook OH ◽  
Se-Ho PARK ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Promoter Activity ◽  
Expression System ◽  
Critical Role ◽  
Minimal Promoter ◽  
Mobility Shift ◽  
Topoisomerase Iiα ◽  
Dna Topoisomerase ◽  
Specific Regulation ◽  
Dna Topoisomerase Iiα

DNA topoisomerase IIα (topo IIα) is an essential nuclear enzyme required for chromosome segregation during mitosis. Consistent with its critical role in cell division is the fact that the expression of the gene for topo IIα is strongly regulated by the proliferation state of cells. Using a transient expression system, we determined the contribution of putative cis-acting elements in its promoter region to its basal level and cell proliferation-dependent transcription. Experiments with 5′ and/or 3′ serial deletion and site-directed mutation revealed that (1) maximal promoter activity resides in the fragment extending to position -663 bp from the ATG initiation codon, (2) minimal promoter activity is harboured at -195 bp, (3) the defined minimal promoter contains only two putative elements, inverted CCAAT box 4 (ICB4) (-166 to -162 bp) and the most proximal GC-rich box in the promoter (GC2) (-149 to -143 bp), and (4) ICB4 is most important in the basal-level transcription of the gene for rat topo IIα. The luciferase activities of the mutated reporter plasmids in G0-arrested and exponentially growing cells showed that proliferation-specific regulation is controlled mainly by GC2. Electrophoretic mobility-shift assays indicated that Sp1 binds specifically to the GC2 site. The extent of DNA-protein complex formation increases after the stimulation of cells to proliferate. These results indicate that the increased binding activity of Sp1 to GC2 is important in the up-regulation of the gene for topo IIα in growing cells.

scholarly journals Probing Conformational Changes in Human DNA Topoisomerase IIα by Pulsed Alkylation Mass Spectrometry

2012 ◽  
Vol 287 (30) ◽  
pp. 25660-25668 ◽  
Author(s):  
Yu-tsung Chen ◽  
Tammy R. L. Collins ◽  
Ziqiang Guan ◽  
Vincent B. Chen ◽  
Tao-Shih Hsieh
Keyword(s):  
Mass Spectrometry ◽  
Conformational Changes ◽  
Topoisomerase Iiα ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Dna Topoisomerase Iiα

ATF is important to late S phase-dependent regulation of DNA topoisomerase IIα gene expression in HeLa cells

2002 ◽  
Vol 184 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Mee-Young Son ◽  
Tae-Jeong Kim ◽  
Kwang-In Kweon ◽  
Jong-Il Park ◽  
Chung Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hela Cells ◽  
S Phase ◽  
Topoisomerase Iiα ◽  
Dna Topoisomerase ◽  
Dna Topoisomerase Iiα

Discovery of new ATP-competitive inhibitors of human DNA topoisomerase IIα through screening of bacterial topoisomerase inhibitors

2020 ◽  
Vol 102 ◽  
pp. 104049
Author(s):  
Žiga Skok ◽  
Martina Durcik ◽  
Darja Gramec Skledar ◽  
Michaela Barančoková ◽  
Lucija Peterlin Mašič ◽  
...  
Keyword(s):  
Topoisomerase Inhibitors ◽  
Topoisomerase Iiα ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Competitive Inhibitors ◽  
Dna Topoisomerase Iiα
Sign in / Sign up

Export Citation Format

Share Document