scholarly journals In vitro activity of pomegranate peel extract alone and in electrospun poly(vinyl alcohol)/sodium alginate matrix

2020 ◽  
Vol 17 (2) ◽  
pp. 1-19
Author(s):  
Sanderson Vieira Batista ◽  
Thais Martins da Silva ◽  
Sthefany Brito Salomão ◽  
Kamila Arêas Bastos ◽  
Sarah Oliveira Lamas de Souza ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Wound Dressings ◽  
Diffusion Method ◽  
Poly Vinyl Alcohol ◽  
Bacterial Strains ◽  
Pomegranate Peel ◽  
Electrospinning Technique ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Wound dressings can support healing by acting as physical barriers and drug delivery systems. The present study aimed to evaluate the biological activity of films containing pomegranate peel extract at 1.25% w/w prepared by the electrospinning technique for use as wound dressing. The extract was subjected to an in vitro cytotoxicity assay using fibroblasts. The formation of fibers was confirmed using scanning electron microscopy. Biocompatibility was analyzed by determining the hemolysis index, while the susceptibility of bacterial strains and a fungus was investigated using the disc diffusion method. The extract proved to be nontoxic with cell proliferation occurring at a concentration > 30 µg·mL-1. Photomicrograph images confirmed the formation of nanofibers. Films without or with the extract showed good hemocompatibility. The antimicrobial activity of films loaded with extract was demonstrated by the formation of zones of inhibition of Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans, suggesting that the release of the extract in the medium occurred at an adequate concentration to inhibit the proliferation of microorganisms. No zone of inhibition was observed for Escherichia coli. These results suggest that the film produced by electrospinning containing 1.25% w/w of the extract might serve as a bioactive wound dressing.

Development and Evaluation of a Novel Oral Mucoadhesive Ointment Containing Pomegranate Peel Extract as an Adjuvant for Oral Hygiene of Dogs

2020 ◽  
Vol 37 (3) ◽  
pp. 133-140
Author(s):  
Thais M. Silva ◽  
Thays C. A. Bolzan ◽  
Marcos S. Zanini ◽  
Taiana Alencar ◽  
Winner D. Rodrigues ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Oral Hygiene ◽  
Diffusion Method ◽  
Pomegranate Peel ◽  
Ointment Formulation ◽  
Microbiological Testing ◽  
Herbal Therapies ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Herbal therapies are used worldwide to treat a variety of health conditions, including dental conditions in veterinary medicine. In this context, the use of medicinal plant-based formulations as potential therapeutics and preventatives in veterinary dentistry is worth highlighting. The objective of the present study was to develop a mucoadhesive ointment formulation, named orabase, that contained pomegranate extract for use in the oral cavity of dogs, with the aim of improving their oral hygiene. The hydroalcoholic extracts of pomegranate peels was incorporated into the orabase in 3 different concentrations. The formulations were subjected to in vitro microbiological testing by a modified disc-diffusion method to study the susceptibility of microorganisms collected from the oral cavities of the dogs. The samples were taken from the buccal mucosa of dogs having the same management and diet. The most effective formulation was submitted to physicochemical tests to evaluate the functionality of the product, namely pH, swelling index, spreadability, and mechanical properties (hardness, cohesiveness, and adhesiveness). The formulation containing 25.0% w/w of the extract was considered most suitable for the intended use as it showed antiseptic activity and demonstrated a swelling index of approximately 35% in the first 20 minutes of the test, high spreadability, and suitable mechanical properties. The results suggest that the product obtained from pomegranate peel extract is a viable option for use to improve oral hygiene, helping to reduce the bacterial component of dental plaque in dogs.

Polymeric films containing pomegranate peel extract based on PVA/starch/PAA blends for use as wound dressing: In vitro analysis and physicochemical evaluation

2020 ◽  
Vol 109 ◽  
pp. 110643 ◽  
Author(s):  
Nubya Nascimento Costa ◽  
Lohanna de Faria Lopes ◽  
Daiana Freitas Ferreira ◽  
Elisabeth Maria López de Prado ◽  
Juliana Aparecida Severi ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Polymeric Films ◽  
Pomegranate Peel ◽  
Physicochemical Evaluation ◽  
Pomegranate Peel Extract ◽  
Vitro Analysis ◽  
Peel Extract ◽  
In Vitro Analysis

scholarly journals Pomegranate Peel Extract Is a Potential Alternative Therapeutic for Giardiasis

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 705
Author(s):  
Asmaa M. El-Kady ◽  
Iman A. M. Abdel-Rahman ◽  
Samer S. Fouad ◽  
Khaled S. Allemailem ◽  
Taghrid Istivan ◽  
...  
Keyword(s):  
Diarrheal Disease ◽  
Apoptotic Cell ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Control Group ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Multiple Drugs ◽  
Peel Extract

Giardiasis is a major diarrheal disease affecting approximately 2.5 million children annually in developing countries. Several studies have reported the resistance of Giardia lamblia (G. lamblia) to multiple drugs. Therefore, identifying an effective drug for giardiasis is a necessity. This study examined the antiparasitic effect of Punica granatum (pomegranate) and evaluated its therapeutic efficacy in rats infected with G. lamblia. In vitro study showed high efficacy of pomegranate peel ethanolic extract in killing G. lamblia cysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number of G. lamblia cysts and trophozoites in feces and intestine respectively. Interestingly, the number of G. lamblia trophozoites and cysts were significantly lower in the pomegranate extract-treated group compared to the metronidazole-positive control group. Moreover, pomegranate extract treatment significantly induced nitric oxide (NO) and reduced serum IL-6 and TNF-α, compared to infected untreated rats. Histological and scanning electron microscopy (SEM) examination of the jejunum and duodenum of pomegranate extract-treated animals confirmed the antiparasitic effect of the extract, and demonstrated the restoration of villi structure with reduction of villi atrophy, decreased infiltration of lymphocytes, and protection of intestinal cells from apoptotic cell death. In conclusion, our data show that the pomegranate peel extract is effective in controlling G. lamblia infections, which suggests that it could be a viable treatment option for giardiasis.

scholarly journals Pomegranate Peel Extract Polyphenols Attenuate the SARS-CoV-2 S-glycoprotein Binding Ability to ACE2 Receptor: In Silico and In Vitro Studies

2021 ◽  
pp. 105145
Author(s):  
Relja Suručić ◽  
Maja Travar ◽  
Miroslav Petković ◽  
BiljanaTubić ◽  
Miloš P. Stojiljković ◽  
...  
Keyword(s):  
In Silico ◽  
In Vitro Studies ◽  
Pomegranate Peel ◽  
Binding Ability ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Anti-Candida Activity of Pomegranate Peel Extract in Comparison with Curcumin Extract and their Synergism with Fluconazole and Nystatin

2021 ◽  
Vol 30 (2) ◽  
pp. 81-87
Author(s):  
Sara Y. Maxwell ◽  
Sally Elnawasany ◽  
Azza M. Hassan ◽  
Marwa M. E. Abd-Elmonsef
Keyword(s):  
Candida Species ◽  
Synergistic Effects ◽  
Systemic Disease ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Oropharyngeal Candidiasis ◽  
Pomegranate Peel ◽  
Methanolic Extracts ◽  
Pomegranate Peel Extract

Background: Oropharyngeal candidiasis is an important sign that may reflect a serious systemic disease, especially in immunocompromised patients who face the intolerable side effects of the available antifungal drugs. This necessitates the development of safe and effective natural components. Objectives: to evaluate the in vitro activities of both pomegranate peel and curcumin extracts and to compare them with nystatin and fluconazole drugs against Candida species. As far as we know, this is the first study comparing between the antifungal potency of both extracts. Methodology: Different Candida species were isolated from patients with oropharyngeal candidiasis. The antifungal activities of methanolic extracts of pomegranate peel and curcumin were tested by disc diffusion method. Both extracts were added to each of nystatin and fluconazole discs to measure their synergistic effects. Results: Highly significant synergism was detected between both extracts and each of antifungal drugs. Curcumin extract was more potent than pomegranate extract. Conclusion: When used in combination with nystatin and fluconazole, curcumin and pomegranate peel extracts are promising and effective anti-Candida agents.

scholarly journals Effectiveness of a pomegranate peel extract (PGE) in reducing Listeria monocytogenes in vitro and on fresh-cut pear, apple and melon

2020 ◽  
Vol 246 (9) ◽  
pp. 1765-1772
Author(s):  
I. Belgacem ◽  
L. Schena ◽  
N. Teixidó ◽  
F. V. Romeo ◽  
G. Ballistreri ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Pomegranate Peel ◽  
Fresh Cut ◽  
Pomegranate Peel Extract ◽  
Peel Extract

scholarly journals Effects of Pomegranate Peel (Punica granatum L.) Extract as an Anthelmintic

2017 ◽  
Vol 1 (5) ◽  
Author(s):  
Monica Amelia ◽  
Diana K Jasaputra ◽  
Rita Tjokropranoto
Keyword(s):  
Rural Areas ◽  
Ascaris Suum ◽  
Pomegranate Peel ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Pomegranate Peel Extract ◽  
Peel Extract ◽  
Anthelmintic Effect

Helminths infections caused by Soil-Transmitted Helminths (STH) are found in manypeople living in developing countries, especially in rural areas. People often use pomegranate asan anthelmintic. The aim of this research is to find out the effects of pomegranate peel (Punicagranatum L.) as an anthelmintic to female Ascaris suum in vitro. The research on the effects ofpomegranate peel extract has been conducted on 900 female Ascaris suum in vitro. Ascaris suumare divided into 5 groups, group I: pomegranate peel extract of dose 25%, group II: pomegranatepeel extract of 50%, group III: pomegranate peel extract of 75%, group IV: 0.9% NaCl as anegative control, and group V is given mebendazole 0.5% as a positive control. The meanpercentage of dead worms in group I is 39%, in group II 61%, while in group III 82%, but itspotential is lower than mebendazole, which kills 100%. The treatment using pomegranate peelextract of 25%, 50% and 75% respectively has significant differences with p < 0.05 againstnegative control (NC) using a 0.9% NaCl. The research concludes that the pomegranate peelextract has an anthelmintic effect against Ascaris suum females in vitro.Keywords: pomegranate peel extract, anthelmintic effect, in vitro

Effect of Pomegranate Peel Extract onCandida albicansGrowth and Biofilm Formation

2018 ◽  
Vol 17 (3) ◽  
pp. 120-128
Author(s):  
H. Merzouk ◽  
B. Bedja ◽  
B. Benmeziane ◽  
N. Touati ◽  
M. Chibane
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Qualitative Assessment ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Fruit Peel ◽  
Pomegranate Peel ◽  
Total Polyphenols ◽  
Pomegranate Peel Extract ◽  
Peel Extract

In the present work, we studied the potential antioxidant capacity and antibiofilm of pomegranate peel extract of two cultivars againstCandida albicansstrains. First, both pomegranate peel extracts were evaluated to determine their total phenolic and tannin contents. Their antioxidant activity was investigated using hydrogen peroxide scavenging assay. Acid fruit peel extract showed the most significant levels of total polyphenols (513.78 ± 1.552 mg GAE/g DE) and possessed the strongest antioxidant ability (96.32%). Next, the possible effect of the extracts onCandida albicansstrains was studied using agar diffusion method and subjected to various extracts concentrations. The zone sizes of growth inhibition showed clearly that these extracts had a good antifungal activity againstCandida albicansspecies in the following order: CA1 > CA3 > CA2 with minimum inhibitory concentrations (MICs) in the range 0.97–1.94 mg/ml. Images obtained by scanning electron microscopy allowed for a qualitative assessment of the biofilm reduction.

Phenolic-rich Pomegranate Peel Extract: In Vitro, Cellular, and In Vivo Activities for Skin Hyperpigmentation Treatment

Planta Medica ◽  
2020 ◽  
Vol 86 (11) ◽  
pp. 749-759
Author(s):  
Mayuree Kanlayavattanakul ◽  
Wichayada Chongnativisit ◽  
Puxvadee Chaikul ◽  
Nattaya Lourith
Keyword(s):  
Sinapic Acid ◽  
Inhibitory Effect ◽  
Dermal Fibroblasts ◽  
Pomegranate Peel ◽  
Double Blind ◽  
Skin Hyperpigmentation ◽  
Pomegranate Peel Extract ◽  
Skin Lightening ◽  
Peel Extract

AbstractThe pomegranate phenolics are reported to have cutaneous benefits and to be effective in treating skin disorders, including hyperpigmentation. In this context, a preparation method was developed by which to obtain phenolic-rich pomegranate peel extract. Sinapic acid was presented as the major pomegranate peel phenolics, followed by gallic and ellagic acids, and 4 additional phenolics. The extract exhibited strong antioxidant activity with an in vitro tyrosinase inhibitory effect. The skin hyperpigmentation treating potency was confirmed by the suppression of cellular melanogenesis through tyrosinase and TRP-2 inhibitions as examined in the B16F10 melanoma cells. Cellular antioxidant and proliferative activities of the extract toward human dermal fibroblasts were evidenced, as well as an inhibitory effect against MMP-2. The extract was developed into the stable serum and mask. The products were proved to be non-irritated in 30 Thai volunteers participating in a single application closed patch test. A split-face, randomized, double-blind, placebo-controlled test of the skin lightening effect was evaluated in the 30 volunteers over 28 consecutive daily treatments and monitored by the Mexameter MX 18. The active serum and mask were better in facial skin lightening efficacy than the placebo (p < 0.005). That was in accordance with the sensory evaluation scored by the volunteers. Phenolic-rich pomegranate peel extract is evidenced as a safe herbal derived material promising for skin hyperpigmentation treatment. Supportive information regarding chemical and biological profiles is presented with the confirmed safety and cutaneous benefits in volunteers.

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