scholarly journals Séroépidémiologie de la babésiose bovine à Babesia divergens en Tunisie

Author(s):  
A. Bouattour ◽  
M. Ghammam ◽  
Mohamed Aziz Darghouth ◽  
S. Touil ◽  
M. Tahri ◽  
...  

La mise en évidence de Babesia divergens chez un bovin autochtone en Tunisie est récente. Une étude sérologique et parasitaire effectuée dans sept différentes localités à biotope favorable à Ixodes ricinus a permis de confirmer la présence de cette Babesia dans plusieurs troupeaux. Au total, 307 bovins de différents âges, répartis dans ces zones, ont été testés par immunofluorescence indirecte en utilisant comme antigène les globules rouges de la gerbille (Meriones unguiculatus) infectés par B. divergens. La prévalence globale de l’infection avec des sérums dilués à 1/80 a été de 44,6 p. 100. Cette prévalence a été variable en fonction de la localité, du troupeau et de la catégorie d’âge. L’examen des frottis confectionnés à partir de sang hépariné de ces bovins a révélé la présence de piroplasmes de petite taille chez 28,3 p. 100 des animaux. L’inoculation de 46 prélèvements aux gerbilles a permis d’isoler deux souches de B. divergens. Ces résultats montrent que B. divergens, considérée jusqu’à présent comme se cantonnant à l’Europe, est présente en Afrique du Nord sous forme de foyers et qu’une endémie stable s’est établie entre cet hémoparasite et les bovins de race locale (Bos taurus).

Parasitology ◽  
2006 ◽  
Vol 134 (2) ◽  
pp. 197-207 ◽  
Author(s):  
S. BONNET ◽  
M. JOUGLIN ◽  
L. MALANDRIN ◽  
C. BECKER ◽  
A. AGOULON ◽  
...  

Although Babesia divergens is the the principal confirmed zoonotic Babesia sp. in Europe, there are gaps in our knowledge of its biology and transmission by the tick Ixodes ricinus. In order to reproduce the part of the parasite cycle that occurs in the vector, an in vitro animal skin feeding technique on blood containing in vitro cultivated B. divergens was developed. Parasite DNA was detected in all samples of salivary glands of nymphs and adults that had fed on parasitized blood as larvae and nymphs, respectively, indicating acquisition as well as a transtadial persistence of B. divergens. PCR performed on eggs and larvae produced by females that had fed on parasitized blood demonstrated the existence of a transovarial transmission of the parasite. Gorging B. divergens infected larvae on non-infected gerbils showed persistance of the parasite over moulting into the resulting nymphs. These results indicate that the parasitic stages infective for the vector (i.e. the sexual stages) can be produced in vitro. To our knowledge, this is the first report of artificial feeding of I. ricinus via membrane as well as in vitro transmission of B. divergens to its vector. The opportunities offered by the use of such a transmission model of a pathogen by I. ricinus are discussed.


2012 ◽  
Vol 185 (2-4) ◽  
pp. 101-109 ◽  
Author(s):  
Albert Agoulon ◽  
Laurence Malandrin ◽  
Florent Lepigeon ◽  
Maxime Vénisse ◽  
Sarah Bonnet ◽  
...  

1963 ◽  
Vol 14 (3) ◽  
pp. 367-373 ◽  
Author(s):  
L.P. Joyner ◽  
S.F.M. Davies ◽  
S.B. Kendall

Parasitology ◽  
2017 ◽  
Vol 144 (6) ◽  
pp. 707-711 ◽  
Author(s):  
SOLANGE OLIVEIRA ◽  
RODRIGO MARTINS SOARES ◽  
JULIANA AIZAWA ◽  
HERBERT SOUSA SOARES ◽  
DANIELA PONTES CHIEBAO ◽  
...  

SUMMARYThe biological and genetic diversity of Neospora caninum is very limited because of availability of only a few viable isolates worldwide. This study describes the isolation and biological and molecular characterization of a new viable isolate of N. caninum (NC-SP1), from a cattle in Brazil. Approximately 400 g of brain from a naturally infected adult male cattle from an abattoir was fed to a 2-month-old dog. Neospora-like oocysts were observed on day 7 post-inoculation (PI) and the duration of oocyst shedding was 14 days. The DNA obtained from oocysts was characterized molecularly and the final sequence was 99% identical to homologous sequences of N. caninum available in GenBank®. For bioassay, gerbils (Meriones unguiculatus) were orally inoculated with 10 100 and 1000 oocysts; all gerbils remained clinically normal but developed N. caninum antibodies 14 days PI. Cell culture isolation was successful using the brain homogenate from one of the gerbils and tachyzoites were observed 24 days PI. Microsatellite genotyping revealed a unique genetic profile for this new reference isolate.


2020 ◽  
Vol 43 (2) ◽  
pp. 185-191
Author(s):  
Lola Romanos ◽  
Renaud Pierre Maillard

AbstractTick-borne diseases are highly prevalent in domestic and wild ruminants and they may be distributed in wide geographical ranges by animal transportation. The aim of the current study was to investigate the presence of European strains of Babesia spp. and/or Anaplasma spp. in oversea imported reindeer specimens. Imported specimens (n=7) were hospitalized with visible tick infestation (Ixodes ricinus) and signs of cachexia, anemia, and hemoglobinuria. Using blood smears, PCR, and BLAST comparisons, it was confirmed that the animals were infected with a French strain of Anaplasma phagocytophilum and Babesia divergens which is considered to be absent in the USA. We conclude that oversea importation of reindeers must be followed with a routine check for geographically-specific strains of pathogens from the place of origin. This monitoring process must be dynamic and according to recent reports of tick-borne pathogens.


Sign in / Sign up

Export Citation Format

Share Document