scholarly journals Pemberian Serbuk Biji Kelor pada Limbah Cair Rumah Pemotongan Ayam Tradisional Mampu Menurunkan Jumlah Escherichia coli

2020 ◽  
Vol 9 (2) ◽  
pp. 148-156
Author(s):  
I Dewa Ketut Ari Saputra ◽  
I Ketut Suada ◽  
I Made Merdana
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
E Coli ◽  
Split Time

Serbuk biji kelor dapat berperan sebagai koagulan alami untuk memperbaiki kualitas air, mereduksi logam berat, menurunkan jumlah bakteri Escherichia coli dan alga serta sebagai surfaktan / hidrofilik. Penelitian ini bertujuan untuk mengetahui pengaruh serbuk biji kelor pada konsentrasi berbeda terhadap jumlah E. coli pada limbah Rumah Pemotongan Ayam tradisional (RPA). Pada penelitian ini menggunakan Rancangan Acak Kelompok (RAK) pola split-time. Bakteri E. coli dihitung dengan metode penumbuhan pada media EMBA (eosin methylene blue agar). Faktor utama yaitu konsentrasi serbuk biji kelor sebagai berikut ; 0 mg / L, 50 mg / L, 100 mg / L, 150 mg / L dan 200 mg / L, dengan faktor tambahan adalah waktu pengendapan yaitu 0 menit, 20 menit, 40 menit, 60 menit. Hasil sidik ragam menunjukkan bahwa serbuk biji kelor berpengaruh sangat nyata terhadap jumlah bakteri E. coli pada limbah RPA tradisional. Dengan konsentrasi terbaik serbuk biji kelor, 200 mg/L dan lama pengendapan yakni 60 menit, memberikan pengaruh terhadap penurunan jumlah E. coli pada limbah RPA tradisional.

scholarly journals Removal of Methylene blue, Escherichia coli and Pseudomonus aeruginosa by Adsorption Process of Activated Carbon Produced from Moringa oleifera Bark

2021 ◽  
Author(s):  
Md. Shahin Azad ◽  
Syaza Azhari ◽  
Mohd Sukri Hassan
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Activated Carbon ◽  
Moringa Oleifera ◽  
Adsorption Process ◽  
Diffusion Mechanism ◽  
Bacterial Load ◽  
Batch Adsorption ◽  
Maximum Adsorption Capacity ◽  
E Coli

The utilization of biopolymer derived from Moringa oleifera bark using ZnCl2 and H2SO4 as activating agents for eliminating Methylene blue, Escherichia coli and Pseudomonas aeruginosa from producing wastewater. In this study, Methylene blue and both bacteria were effectively adsorbed by activated carbon with lowest dosage. The activated carbon was prepared from natural-by product of Moringa oleifera bark by pyrolysis in a furnace at 700°C for 1 h. The characteristics of activated carbon have been determined using Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET), pHzpc (zero point charge), and FTIR spectroscopy. The obtained result were closely fitted with Freundlich isotherm model and adsorption kinetics follow the pseudo-second order model with the highest value of correlation coefficient (R2~1). Adsorption quantity was dose dependent and bacteria were maximum adsorbed using 10 mg of activated carbon as well as 25mg for methylene blue. The maximum adsorption capacity showed within 1 hour. The bacterial load was reduced by 98% for E. coli, 96% for P. aeruginosa as well as methylene blue reduced 94.2% from aqueous solution using batch adsorption methods. Adsorption process controlled by film diffusion mechanism. These result proposed that the activated carbon of Moringa oleifera can be used as a good adsorbent for the removal of Methylene blue, E. coli and P. aeruginosa.

Comparison of the β-Glucuronidase Assay and the Conventional Method for Identification of Escherichia coli on Eosin-Methylene Blue Agar

1997 ◽  
Vol 60 (1) ◽  
pp. 6-9 ◽  
Author(s):  
SHIU W. HUANG ◽  
CHUNG H. CHANG ◽  
TUNG F. TAI ◽  
TSUNG C. CHANG
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Conventional Method ◽  
Meat Products ◽  
Mixed Cultures ◽  
Effective Alternative ◽  
Methyl Red ◽  
Raw Meat ◽  
E Coli ◽  
Glucuronidase Assay

The purpose of this study was to compare the IMViC (indole, methyl red, Voges-Proskauer and citrate utilization) tests with the β-glucuronidase (GUD) assay for the identification of suspect Escherichia coli on Levine's eosin-methylene blue (EMB) agar. After testing 258 suspect E. coli colonies from raw meat and meat products, 163 and 44 were found to be E. coli and non-E. coli, respectively, by both methods. Nine isolates were IMViC positive (i.e., + + − − or − + − −) but GUD negative; among these isolates, six were confirmed to be E. coli by API 20E (bioMérieux, Marcy-I'Etoile, France) with the remaining three being non-E. coli. There were 42 isolates that were IMViC negative but GUD positive; among these isolates, seven were pure E. coli cultures, 33 were mixed cultures containing E. coli, and the remaining two were Proteus spp. The sensitivities for the identification of E. coli on EMB were 80.9% (169/209) and 97.1% (203/209), respectively, by the IMViC tests and GUD assay; whereas the specificities were 93.9% (46/49) and 95.9% (47/49), respectively, by the IMViC tests and GUD assay. It is proposed that the GUD assay can be an effective alternative to the conventional IMViC tests for the identification of suspect E. coli on EMB.

Differently ordered carbonaceous structures synthesized by bubbled Ar or He plasmas inside methylene blue solutions with contrasting Escherichia coli growth inhibition effects

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98325-98334 ◽  
Author(s):  
D. Zaharie-Butucel ◽  
J. Papp ◽  
C. Leordean ◽  
S. D. Anghel
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Growth Inhibition ◽  
E Coli ◽  
Structure Synthesis ◽  
Coli Growth

A straightforward carbonaceous structure synthesis from methylene blue aided by Ar/He plasmas with different ordering and E. coli inhibition effects.

scholarly journals Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

2020 ◽  
Vol 10 (2) ◽  
pp. 106
Author(s):  
Dian Meididewi Nuraini ◽  
Morsid Andityas ◽  
Adi Paramarta ◽  
Nur Rohman Najib ◽  
Agustina Dwi Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Drinking Water ◽  
Inhibitory Activity ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Test ◽  
Aloe Barbadensis ◽  
E Coli

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%.  Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity   Abstrak  Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%.  Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

scholarly journals Hubungan Higiene, Fasilitas dan Sanitasi Lingkungan dengan Kualitas Mikrobilogi Serta Identifikasi Eschericia Coli O157: H7 Pada Sate Languan

2019 ◽  
Vol 18 (2) ◽  
pp. 104
Author(s):  
Sang Gede Purnama ◽  
Made Subrata
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Total Sample ◽  
Raw Material ◽  
Food Handler ◽  
Environmental Sanitation ◽  
E Coli ◽  
Sequential Explanatory Design ◽  
Sequential Explanatory ◽  
Surrounding Areas

Latar belakang: Proses pembuatan sate languan yang dari bahan baku ikan rentan mengalami kontaminasi secara mikrobiologi akibat kontaminasi alat luluh yang kurang dibersihkan dan penjamah makanan. Oleh sebab itu, perlu dilakukan identifikasi cemaran dan faktor risikonya.Metode: penelitian ini menggunakan metode campuran (mixed methode) dengan rancangan penelitian sequential explanatory design yakni menggabungkan dua bentuk penelitian yakni kuantitatif dan kualitatif dimana pada tahap pertama mengambil data kuantitatif selanjutnya data kualitatif. Pendekatan kuantitatif juga dilakukan dengan observasi secara langsung oleh peneliti.  Jumlah sampel seluruh pedagang Sate Languan di Pantai Lebih dan sekitarnya sebanyak 19 rumah makan dan 19 sampel Sate Languan.  Variabel yang diteliti higiene penjamah makanan, kepemilikan alat luluh, ketersediaan fasilitas sanitasi,  sanitasi lingkungan, lama kerja dan pendidikan. Pemeriksaan mikrobiologi dengan identifikasi Eschericia coli O157:H7 dengan pembiakan pada media eosin methylene blue agar (EMBA), identifikasi  E. coli O157:H7 dilanjutkan dengan penumbuhan isolate bakteri di media selektif sorbitol MacConkey agar (SMAC) dilanjutkan dengan uji konfirmasi dengan lateks O157.Hasil: variabel yang berhubungan dengan kontaminasi Eschericia colipada sate languan yakni higiene penjamah makanan, sarana fasilitas sanitasi dan kepemilikan alat luluh. Higiene penjamah makanan yang termasuk kategori kurang baik sebanyak (79%), kategori fasilitas sanitasi tidak memadai sebanyak (53%), kategori sanitasi lingkungan kurang bersih sebanyak (47%). Dari 19 sampel yang dilakukan pemeriksaan coliform dan E. coli diketahui 15 warung makanterkontaminasi E. coli rata-rata 5 x 106 cfu/gram hanya  4 warung yang E. coli masih dalam batas aman. Hasil uji lanjutan diketahui bahwa negatif Eschericia coli O157:H7.Simpulan : ada hubungan higiene penjamah makanan, sarana fasilitas sanitasi dan kepemilikan alat luluhdengan kontaminasi E. coli. Ditemukan cemaran E. coli pada makanan Sate Languan namun tidak terbukti jenis E. coliO157:H7. ABSTRACTTitle: The Relationship of Hygiene, Facilities and Environmental Sanitation with The Identification of Eschericia Coli O157: H7 at Languan Satay.Background: The process of processing satay from raw material from chopped fish is susceptible to microbiological contamination due to unclean contamination of equipment and food handlers. Therefore, it is necessary to identify contaminants and risk factors.Method: This study uses (mixed method) a sequential explanatory design that combines two forms of research, namely quantitative and qualitative, where in the first stage, the quantitative data is then taken from qualitative data. The quantitative approach is also carried out by direct observation by the researcher. The total sample of all satay traders in Lebih Beach and surrounding areas are 19 restaurants and 19 samples of Languan Satay. The variables studied were food handler hygiene, ownership of meat crusher, availability of sanitation facilities, environmental sanitation, length of work and education. Microbiological examination with identification of Escherichia coli O157: H7 with culture on eosin methylene blue agar (EMBA) media, identification of E. coli O157: H7 followed by growth of bacterial isolates in selective Sorbitol Mac Conkey agar (SMAC) followed by confirmation test with O157 latex.Results: variables related to Escherichia coli contamination in satay is food handler hygiene, sanitation facilities and ownership of meat crusher. Food handler hygiene included in the poor category (79%), inadequate sanitation facilities category (53%) and less clean environmental sanitation category (47%). Of the 19 samples that were examined for coliform and E. coli, it was found that 15 stalls contaminated with E. coli averaged 5 x 106 cfu / gram, only 4 stalls which were still within safe limits. The results of the follow-up test revealed that negative Eschericia coli O157: H7.Conclusion:There is a relationship between food handler hygiene, sanitation facilities and ownership of tool with E. coli contamination.It was found E. coli contamination in Languan Satay but it was not proven to be E. coli O157: H7. 

Isolation and partial characterization of an Escherichia coli mutant resistant to colicin A

1975 ◽  
Vol 21 (10) ◽  
pp. 1595-1601 ◽  
Author(s):  
Marc Lavoie ◽  
Leo G. Mathieu
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Parent Strain ◽  
General Pattern ◽  
Sodium Dodecyl ◽  
Triphenyltetrazolium Chloride ◽  
E Coli ◽  
Receptor Sites ◽  
Acridine Dyes

An Escherichia coli K12 mutant resistant to colicin A-CA31 apparently through loss of its receptor sites has been isolated and partially characterized. Resistance to colicin A was accompanied with a decreased sensitivity to colicins L-398 and E2-CA42, and to acridine dyes. The mutant strain displayed the same general pattern of tolerance or sensitivity as the parent strain towards eight antibiotics, colicins C, D, E1, E3, F2, F3, G, I, K, and N; phages T1, T2, T5, T6, T7, F2, λ vir, P1kc, [Formula: see text] 80, and BF23; and to methylene blue, triphenyltetrazolium chloride, ethylene-diaminetetraacetate (EDTA), deoxycholate, and sodium dodecyl sulfate. Conjugation and transduction experiments showed that a locus controlling resistance to colicin A-CA31 mapped at 21 min on the genetic map of this E. coli K12 strain.

scholarly journals IDENTIFIKASI Escherichia coli O157:H7 PADA SUSU SAPI PERAH DAN LINGKUNGAN PETERNAKAN

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Joshua Liem Tiong Gie ◽  
Yatri Drastini
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Brilliant Green ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Cooling Unit

Tujuan penelitian adalah mengidentifikasi Eschericia coli (E. coli) O157:H7 pada susu dan lingkungan peternakan sapi perah. Sampel berjumlah 77 yang terdiri atas 27 sampel susu dan 50 sampel lingkungan. Sampel susu berasal dari ambing sapi (14), milk can peternak (6), milk can tempat penampungan susu (4), dan cooling unit di koperasi (3). Sampel lingkungan berupa feses (14), air sumber dan air tandon (12), pakan (6), serta swab tangan sebelum dan sesudah pemberian minyak pelicin (17), dan tanah (1). Isolasi E. coli dari sampel menggunakan media pemerkaya kaldu brilliant green lactose bile Broth (BGLB), media selektif agar eosin methylene blue (EMB), dan agar sorbitol MacConkey (SMAC). Koloni bakteri yang tidak memfermentasi sorbitol pada SMAC (colorless) diidentifikasi dengan uji aglutinasi lateks O157 dan antisera H7. Identifikasi bakteri dari sampel susu menunjukkan 7,41% (2/27) sampel teridentifikasi E. coli O157. Susu tersebut berasal dari ambing sapi dan milk can peternak. Bakteri E. coli O157 yang teridentifikasi dari sampel lingkungan (sampel pakan) sebanyak 2% (1/50). Hasil uji aglutinasi antisera terhadap tiga sampel positif O157 menunjukkan bahwa ketiganya tidak memiliki antigen H7 dan disimpulkan bahwa tidak ada sampelsusu dan lingkungan yang tercemar E. coli O157:H7.

scholarly journals Deteksi Fenotipik Escherichia coli Penghasil Extended Spectrum Beta-lactamases (ESBLs) pada Sampel Makanan di Krian Sidoarjo

Life Science ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 95-105
Author(s):  
Yulianto Ade Prasetya ◽  
Ike Yuyun Winarsih ◽  
Kharisma Aprilia Pratiwi ◽  
Merinsa Chorry Hartono ◽  
Dita Nur Rochimah
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Food Samples ◽  
Beta Lactamases ◽  
E Coli ◽  
Extended Spectrum ◽  
Confirmation Test ◽  
Extended Spectrum Beta Lactamases ◽  
Fried Foods ◽  
Synergy Test

Escherichia coli is a group of Enterobacteriaceae bacteria that often contaminate food so that it can cause diarrhea. These bacteria are very difficult to treat if they are able to produce the Extended-Spectrum Beta-Lactamases (ESBLs) enzyme. The purpose of this study was to identify ESBLs-producing E. coli in food samples in Krian Sidoarjo. Food samples (fried foods, cilok tempura and chili sauce) were collected from ten different places. The sample was then grown on Eosin Methylene Blue (EMB) medium and purified by the 16 streak method, as well as biochemical character tests. The ESBLs phenotypic E. coli method was carried out by screening test and confirmation test using a Double Disk Synergy Test (DDST). Thirty colonies were able to grow on EMB media, but after microscopic identification and biochemistry testing only four samples were E. coli positive and were able to produce ESBLs from the phenotypic test that had been carried out. ESBLs-producing E. coli testing is important not only for nosocomial infections but also for the community so it needs attention to the spread of ESBLs resistance among microorganism species.   Escherichia coli termasuk kelompok bakteri Enterobacteriaceae yang sering mengkontaminasi makanan sehingga dapat menyebabkan diare. Bakteri ini sangat sulit diobati apabila mampu memproduksi enzim Extended Spectrum Beta-Lactamases (ESBLs). Tujuan penelitian ini adalah untuk mengindetifikasi E. coli penghasil ESBLs pada sampel makanan di Krian Sidoarjo. Sampel makanan (gorengan, cilok, tempura, dan saus sambal) dikumpulkan dari sepuluh tempat berbeda. Sampel kemudian ditumbuhkan pada medium Eosin Metilen Blue (EMB) dan dimurnikan dengan metode streak 16, serta dilakukan karakteristik uji biokimia. Metode fenotipik E. coli penghasil ESBLs dilakukan dengan uji skrining dan uji konfirmasi menggunakan double disk synergy test (DDST). Sebanyak tiga puluh koloni mampu tumbuh pada media EMB, namun setelah diidentifikasi mikroskopis dan uji biokiomia hanya empat sampel positif E. coli dan mampu menghasilkan ESBLs dari uji fenotipik yang telah dilakukan. Pengujian E. coli penghasil ESBLs penting dilakukan bukan hanya pada infeksi nosokomial, tetapi juga pada komunitas sehingga perlu mendapat perhatian terhadap penyebaran resistensi ESBLs diantara spesies mikroorganisme.

Isolation of Shiga Toxin–Producing Escherichia coli from Fresh Produce Using STEC Heart Infusion Washed Blood Agar with Mitomycin-C†

2012 ◽  
Vol 75 (11) ◽  
pp. 2028-2030 ◽  
Author(s):  
ANDREW LIN ◽  
LAM NGUYEN ◽  
LAURIE M. CLOTILDE ◽  
JULIE A. KASE ◽  
INSOOK SON ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Mitomycin C ◽  
Shiga Toxin ◽  
Fresh Produce ◽  
Reference Method ◽  
Blood Agar ◽  
Romaine Lettuce ◽  
E Coli ◽  
Outbreak Investigations

The ability to detect and isolate Shiga toxin–producing Escherichia coli (STEC) remains a major challenge for food microbiologists. Although methods based on nucleic acids and antibodies have improved detection of STECs in foods, isolation of these bacteria remains arduous. STEC isolation is necessary for matching food, environmental, and clinical isolates during outbreak investigations and for distinguishing between pathogenic and nonpathogenic organisms. STEC heart infusion washed blood agar with mitomycin-C (SHIBAM) is a modification of washed sheep blood agar prepared by adding mitomycin-C and optimizing both the washed blood and base agar to better isolate STECs. Most STEC isolates produce a zone of hemolysis on SHIBAM plates and are easily distinguishable from background microbiota. Here, we present data supporting the use of SHIBAM to isolate STECs from fresh produce. SHIBAM was tested for accuracy in identifying STECs (365 of 410 STEC strains were hemolytic, and 63 of 73 E. coli strains that did not produce Shiga toxin were not hemolytic) and for recovery from artificially inoculated fresh produce (11 of 24 romaine lettuce samples and 6 of 24 tomato samples). STEC recovery with SHIBAM agar was greatly improved when compared with recovery on Levine's eosin–methylene blue agar as a reference method.

Resistencia bacteriana de Escherichia coli uropatogénica en población nativa amerindia Kichwa de Ecuador

2017 ◽  
Vol 42 (1) ◽  
pp. 36-45 ◽  
Author(s):  
William M. Guamán ◽  
Víctor R. Tamayo ◽  
José E. Villacís ◽  
Jorge A. Reyes ◽  
Olga S. Munoz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
E Coli ◽  
Vitek 2 ◽  
Para Determinar

Contexto: Escherichia coli uropatógena (ECUP) se presenta como uno de los principales agentes etiológicos en infecciones del tracto urinario (ITUs) no complicadas (70-95%). El objetivo del tratamiento de ITUs no complicadas es obtener curación clínica y microbiológica. Para ello, es de particular importancia el conocimiento de las tasas de resistencia antibiótica local.Objetivo: identificar los perfiles de resistencia a antibióticos de primera línea para ITUs no complicadas en poblaciones nativas amerindias Kichwas ecuatorianas, en donde el tratamiento empírico se basa en trimetoprim/sulfametoxazol, ampicilina, y ciprofloxacina mayoritariamente.Métodos: se analizaron 335 muestras de orina procedentes de las poblaciones de Zumbahua, Colta y Guamote, en un periodo de 4 meses (febrero-mayo 2016). Las muestras fueron incubadas por 24 y 48 horas en agar Eosin Methylene Blue (EMB), para luego ser identificadas en género y especie por pruebasbioquímicas. Para determinar la susceptibilidad antibiótica, se realizó la técnica de difusión en disco de Kirby-Bauer. Para la Concentración Inhibitoria Mínima (CIM), se utilizó la técnica de microdilución en caldo (Vitek 2). El método de doble disco fue la técnica utilizada para la detección de betalactamasas deespectro extendido (BLEE).Resultados: noventa (26,9%) muestras mostraron un recuento significativo de ≥105 (ufc)/ml, compatibles con ITUs. El microorganismo identificado con mayor frecuencia fue E. coli (n=75; 83,3%). La resistencia antibiótica encontrada para los aislados de E. coli fue de 56,7% a trimetoprim/sulfametoxazol, 52,5% aampicilina, 43.3% a ácido nalidíxico, 32.5% a ciprofloxacina, 28.3% a norfloxacina, 25% a levofloxacina, 15.85% a cefazolina, 17.5% a cefoxitina, 15% a cefuroxima, 15% a ceftazidima, cefotaxima, y ceftriaxona, 15% a cefepima, 7,5% a nitrofurantoina y 1,7% a fosfomicina. Se identificaron 7 aislados productores de betalactamasas de espectro extendido (BLEE).Conclusión: con los resultados obtenidos se recomienda no utilizar ampicilina, trimetoprim/sulfametoxazol, ni quinolonas en la zona estudiada como terapia empírica. Se sugiere instaurar tratamiento empírico con fosfomicina o nitrofurantoina para ITUs no complicadas

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