scholarly journals Evaluation of oxidative stress indices after exposure to malathion and protective effects of ascorbic acid in ovarian tissue of adult female rats

10.19082/6789 ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 6789-6795 ◽  
Author(s):  
Somayyeh Abbasabad Arab ◽  
Mohammad Reza Nikravesh ◽  
Mahdi Jalali ◽  
AliReza Fazel
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Adult Female ◽  
Ovarian Tissue ◽  
Female Rats ◽  
Protective Effects ◽  
Stress Indices

Effect of Exposure to Mixture of Heavy Metals Arsenic, Cadmium and Lead on Reproductive Function and Oxidative Stress in Female Rats

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Choudhuri D. ◽  
Bhattacharjee T.
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Ascorbic Acid ◽  
Reproductive Function ◽  
Reproductive Organs ◽  
Female Rats ◽  
Corpora Lutea ◽  
Oestrus Cycle ◽  
Implantation Sites ◽  
Live Fetus

Background : Toxicological consequences arising from exposure to mixtures of heavy metals especially at low, chronic and environmentally relevant doses are poorly recognised. In the present study, we evaluated effects of chronic exposure to combinations of three metals arsenic (As), cadmium (Cd) and lead (Pb) present frequently in drinking water on reproductive function and oxidative damage caused to reproductive organs of female rats. Method : Female rats were exposed to mixture of metals (As, Cdand Pb) for 90 consecutive days. The gain in body weight and weight of reproductive organs were recorded following autopsy on 91 stday. The oestrus cycle were monitored during entire treatment period. Numbers of corpora lutea, implantation sites, live fetus and survival of the fetus were evaluated in rats mated successfully with untreated male after completion of their respective treatment. Ovarian cholesterol, protein, ascorbic acid and enzyme Δ 5 -3β HSD levels were estimated. Serum levels of steroid hormones oestrogen and progesterone were estimated. Histopathological picture of both ovary and uterus were assessed. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidise (GPX) activity, amount of reduced glutathione (GSH) and malondyaldehyde (MDA) in blood, ovary and uterus were measured as biomarkers of oxidative stress. Results : The treated rats showed reduced body weight gain and reduction in the weight of ovary and uterus. Oestrus cycle was disrupted with continuous diestrous in treated animals. Number of corpora lutea, implantation sites and live fetus and the survival of fetus evaluate were reduced significantly in treated groups. The levels of ovarian cholesterol and ascorbic acid increased in treated rats with decrease Δ5 -3β HSD level. There was reduction in serum level of both the ovarian steroid hormones oestrogen and progesterone. The protein levels did not differ between the groups. There was a significant increase in levels of MDA and decrease in levels of all the antioxidant enzymes in treated group. Conclusion : The results revealed there was disruption to reproductive functions with decrease in stereoidogenic activity and associated oxidative stress in female rats treated with combination of mixture of metals (Cd, As and Pb) at low dose for 90 consecutive days.

Eugenol improves tissue damage and oxidative stress in adult female rats after ovarian torsion/detorsion

2021 ◽  
pp. 1-6
Author(s):  
Bita Barghi ◽  
Majid Shokoohi ◽  
Amir Afshin Khaki ◽  
Arash Khaki ◽  
Maryam Moghimian ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Tissue Damage ◽  
Ovarian Torsion ◽  
Female Rats ◽  
And Oxidative Stress

scholarly journals Propolis Protective Effects Against Doxorubicin-Induced Multi-Organ Toxicity via Suppression of Oxidative Stress, Inflammation, Apoptosis, and Histopathological Alterations in Female Albino Rats

2021 ◽  
Vol 12 (2) ◽  
pp. 1762-1777
Keyword(s):  
Oxidative Stress ◽  
Large Scale ◽  
Protective Efficacy ◽  
Biological Activities ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Protective Effects ◽  
Treatment Protocols

Doxorubicin (DOX) is effective chemotherapy in several malignancies, but large-scale toxicities limit its clinical usefulness. Propolis has been reported to exhibit a broad spectrum of biological activities. We aim to assess the protective efficacy of propolis against DOX-induced multi-toxicity in female rats. Forty female rats were divided into four groups: control group; Group (P) were administrated oral propolis (100 mg/kg once daily for 28 days); Group (P+DOX) were injected with a single intraperitoneal dose of DOX (20 mg/kg i.p at 24th day after the propolis administration) and group (DOX) were injected with doxorubicin only. Estimation of cardiac, renal and hepatic injury markers, apoptosis and pro-inflammatory cytokines were done using sera. Also, liver and heart tissue samples were collected to determine GSH and MDA as oxidative stress markers. In addition to histopathological and immunohistochemical examination of Cytochrome-C and Connexin43 on lysed myocardium, liver, kidney and lung tissues. Doxorubicin toxicity caused marked deteriorations of measured parameters through the different mechanisms in different body organs. However, pre-treatment with propolis significantly ameliorated these alterations. Thus propolis can ameliorate the DOX-induced experimental multi-toxicity as cardiomyopathy, hepatotoxicity, nephritis and pneumonia. Thus, it could be a promising protective agent in DOX treatment protocols.

scholarly journals Perinatal taurine depletion increases oxidative stress in adult female rats

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Wichaporn Lerdweeraphon ◽  
James Michael Wyss ◽  
Thidarut Boonmars ◽  
Sanya Roysommuti
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Female Rats

scholarly journals Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

2014 ◽  
Vol 3 (3) ◽  
pp. 18 ◽  
Author(s):  
Mbong Angie M-A ◽  
Djiokeng Paka G. ◽  
Ntentie F. R. ◽  
Dimodi H. ◽  
Ngondi J. L. ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intraperitoneal Administration ◽  
Female Rats ◽  
Protective Effects ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Significant Toxicity ◽  
Cyclophosphamide Treatment ◽  
Hydroethanolic Extract ◽  
Antioxidant Mechanisms

<p>The aim of this study was to evaluate the protective effects of hydroethanolic extracts of <em>S.</em> <em>scabrum</em> and <em>C. verticillata</em> against cyclophosphamide induced toxicity. In this light, female albino wistar rats were treated by intraperitoneal administration of 100 mg/kg BW of cyclophosphamide or distilled water every other day for 7 days associated with oral gavage using hydroethanolic extract of <em>C. verticillata</em>/<em>S. scabrum</em> at a dose of 200 or 400 mg/kg BW or not every day for the same 7 days. On the 8th day, blood and organs (liver, heart and kidney) were collected for analyses of toxicity-related and oxidative stress markers. Cyclophosphamide treatment induced significant toxicity as shown by liver enzymes, urea and creatinine levels. The administration of extracts helped reduce the levels of these markers. The antioxidant effect of these extracts also helped or not to ameliorate oxidative stress markers (MDA, NO, hydroperoxides, catalase, thiols, GPx) depending on the extract and on the dose administered. These results suggest that administration of hydroethanolic extracts of <em>S.</em> <em>scabrum</em> and <em>C. verticillata</em> can help prevent or reduce toxicity that is brought about by treatment with cyclophosphamide due to their ability to upregulate antioxidant mechanisms.</p>

Hepatoprotective Effects of Algerian Crataegus oxyacantha Leaves

2019 ◽  
Vol 10 (1) ◽  
pp. 70-75
Author(s):  
Amira Mecheri ◽  
Wassila Benabderrahmane ◽  
Amel Amrani ◽  
Nassima Boubekri ◽  
Fadila Benayache ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Female Rats ◽  
Protective Effects ◽  
Edible Plant ◽  
Traditional Use ◽  
Butanol Extract ◽  
Hepatic Diseases ◽  
Pharmaceutical Industries ◽  
Hepatoprotective Effects

Background: Hawthorn (C. oxyacantha), a common edible plant, is widely used for the preparation of a different foodstuff and is also used in traditional medicine to treat heart problems and gastrointestinal ailments. Recently, a few patents of Crataegus preparation for protective effects (prevention of cardiovascular and hepatic diseases) have been developed. </P><P> Objective: The current study aimed to explore the antioxidant and hepatoprotective effects of nbutanol extract of Crataegus oxyacantha leaves in acute liver damage induced by Doxorubicin (DOX). </P><P> Methods: Crataegus oxyacantha (100 mg/kg body weight) or vitamin E as a standard antioxidant (100 mg/kg body weight) were administered orally to female rats for 10 days, in the presence or absence of hepatotoxicity induced by a single intraperitoneal (i.p.) injection of DOX (15 mg/kg on the 8th day). On day 11, blood and liver samples were analyzed for biomarker levels and histopathological changes. Liver homogenates were used for determination of oxidative stress parameters that include Malondialdehyde (MDA), Glutathione (GSH) level and Glutathione Peroxidase (GPx) activity. </P><P> Results: Treatment with n-butanol extract of C. oxyacantha leaves significantly improved the altered liver enzyme activities and oxidative stress markers. The histopathological observations confirm the results of biochemical parameters. </P><P> Conclusion: The obtained results support the traditional use of C. oxyacantha to cure gastrointestinal ailments and highlighted its possible use in the food and pharmaceutical industries as a source of natural antioxidant.

Effect of imidacloprid on antioxidant status and histopathological changes in ovary and uterus of adult female wistar rats

2018 ◽  
Author(s):  
Archana Lohiya ◽  
Vinod Kumar ◽  
J.S. Punia
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Wistar Rats ◽  
Control Programme ◽  
Female Rats ◽  
Histopathological Changes ◽  
Gum Acacia ◽  
Flea Control ◽  
Female Wistar Rats ◽  
Neonicotinoid Insecticide

Imidacloprid is a neonicotinoid insecticide and has been extensively used as a crop pest and in pet flea control programme. In the present study, the effects of imidacloprid on ovary and uteri tissue was analyzed in adult female Wistar rats at two dose levels (19 and 38 mg/kg/day) administered orally for 10, 20 and 30 days. Effects were compared with respective control animals administered daily with 2% gum acacia. Parameters undertaken were organ weight, levels of cytoplasmic and membrane proteins, oxidative stress parameters viz. activities of SOD, GPx and levels of GSH and MDA and histopathological changes. IMI (38 mg/kg, 30 days) reduced cytoplamic proteins in both ovaries and uteri whereas this dose reduced membrane protein in ovaries only. IMI (38 mg/kg, 20 and 30 days) decreased SOD enzyme in both ovaries and uteri and GSH-Px levels in ovaries only. The GSH-Px levels were also seen with decreased levels in uteri with IMI (38 mg/kg) for 30 days. IMI (38 mg/kg, 20 and 30 days) induces degenerative changes in ovaries of rats. Hence, it is concluded from the present studies that administration of higher doses (38 mg/kg/day) of IMI for 20 and 30 days generated oxidative stress in ovaries and uteri of female rats.

Effects of ascorbic acid on oxidative stress in ovarian tissue cryopreservation of zebrafish

Cryobiology ◽  
2018 ◽  
Vol 80 ◽  
pp. 182
Author(s):  
Lis Marques ◽  
Shimelly Rocha ◽  
Marcio Hoshiba ◽  
Francielli Santos ◽  
Rajla Simonetti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Ovarian Tissue ◽  
Ovarian Tissue Cryopreservation ◽  
Tissue Cryopreservation

scholarly journals Ginseng Gintonin Attenuates Lead-Induced Rat Cerebellar Impairments during Gestation and Lactation

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 385 ◽  
Author(s):  
Sung Min Nam ◽  
Sun-Hye Choi ◽  
Hee-Jung Cho ◽  
Jin Seok Seo ◽  
Minsuk Choi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Purkinje Cells ◽  
Female Rats ◽  
Protective Effects ◽  
Brain Functions ◽  
Pregnancy And Lactation ◽  
Pb Exposure ◽  
Gaba Transporter 1 ◽  
Developing Cerebellum ◽  
Potential Use

Gintonin, a novel ginseng-derived lysophosphatidic acid receptor ligand, improves brain functions and protects neurons from oxidative stress. However, little is known about the effects of gintonin against Pb-induced brain maldevelopment. We investigated the protective effects of gintonin on the developing cerebellum after prenatal and postnatal Pb exposure. Pregnant female rats were randomly divided into three groups: control, Pb (0.3% Pb acetate in drinking water), and Pb plus gintonin (100 mg/kg, p.o.). Blood Pb was increased in dams and pups; gintonin treatment significantly decreased blood Pb. On postnatal day 21, the number of degenerating Purkinje cells was remarkably increased while the number of calbindin-, GAD67-, NMDAR1-, LPAR1-immunoreactive intact Purkinje cells, and GABA transporter 1-immunoreactive pinceau structures were significantly reduced in Pb-exposed offspring. Following Pb exposure, gintonin ameliorated cerebellar degenerative effects, restored increased pro-apoptotic Bax, and decreased anti-apoptotic Bcl2. Gintonin treatment attenuated Pb-induced accumulation of oxidative stress (Nrf2 and Mn-SOD) and inflammation (IL-1β and TNFα,), restoring the decreased cerebellar BDNF and Sirt1. Gintonin ameliorated Pb-induced impairment of myelin basic protein-immunoreactive myelinated fibers of Purkinje cells. Gintonin attenuated Pb-induced locomotor dysfunctions. The present study revealed the ameliorating effects of gintonin against Pb, suggesting the potential use of gintonin as a preventive agent in Pb poisoning during pregnancy and lactation.

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