scholarly journals Effect of NaHCO3, MgSO4, Sodium Ascorbate, Sodium Glutamate, Phosphate Buffer on Survival of Lactobacillus bulgaricus During Freeze-drying

2013 ◽  
Vol 5 (6) ◽  
pp. 771-774 ◽  
Author(s):  
He Chen ◽  
Juan Wang ◽  
Qian Luo ◽  
Guowei Shu
Keyword(s):  
Phosphate Buffer ◽  
Freeze Drying ◽  
Lactobacillus Bulgaricus ◽  
Sodium Ascorbate ◽  
Sodium Glutamate

scholarly journals Effects of Carbohydrates, Prebiotics and Salts on Survival of Saccharomyces boulardii During Freeze-Drying

2018 ◽  
Vol 22 (2) ◽  
pp. 59-66
Author(s):  
Guowei Shu ◽  
Xin Yang ◽  
Zhangteng Lei ◽  
Dan Huang ◽  
Yaling ZHAI
Keyword(s):  
Survival Rate ◽  
Phosphate Buffer ◽  
Freeze Drying ◽  
Saccharomyces Boulardii ◽  
Sodium Ascorbate ◽  
Sodium Glutamate ◽  
Viable Cells ◽  
Freeze Dried ◽  
Probiotic Yeast ◽  
Addition Amount

Abstract Saccharomyces boulardii, as a probiotic yeast, had been commonly used in food, medicine and feed to treat diarrhea in humans or livestock. However, there are few researches focusing on the preparation of its freeze-drying S.boulardii powder. In this study, the effect of carbohydrates (glucose, sucrose, maltose, fructose, lactose, mannose and trehalose), prebiotics (isomalto-oligosaccharide, xylo-oligosaccharide, raffinose, stachyose, inulin, galacto-oligosaccharide and fructo-oligosaccharide) and salts (NaHCO3, MgSO4, sodium glutamate, sodium ascorbate, and phosphate buffer) on the freeze-dried survival of S. boulardii were investigated to screen the cryoprotectant by using single factor experiments. As the result, trehalose and XOS had better protective effect, the survival rate was 23.72% and 20.70% respectively, the number of viable cells reached 0.91×1010 CFU/g and 0.85×1010 CFU/g respectively; the addition amount of NaHCO3 was 0.3%, the freeze-dried survival rate reached the maximum value of 12.92%. The phosphate buffer additive amount and the bacterial sludge weight were 0.8:1, the freeze-dried survival rate reached a maximum of 14.14%, the freeze-dried survival rate of sodium glutamate, sodium ascorbate and MgSO4 groups was increasing, reaching a maximum of 20.26%, 16.47% and 6.29% when the addition amount was 2%, 10%, 0.5%.

scholarly journals Stability of pseudorabies virus during freeze-drying and storage: effect of suspending media

1976 ◽  
Vol 4 (1) ◽  
pp. 1-5
Author(s):  
E M Scott ◽  
W Woodside
Keyword(s):  
Freeze Drying ◽  
Pseudorabies Virus ◽  
Storage Effect ◽  
Drying Process ◽  
Sodium Glutamate ◽  
Degree Of Protection ◽  
The Stability ◽  
Subsequent Storage ◽  
And Storage ◽  
Lactalbumin Hydrolysate

The effect of suspending media on the stability of pseudorabies virus upon freeze-drying and subsequent storage was studied. A variety of media was tested, including: sodium glutamate; sucrose; lactose; lactalbumin hydrolysate; peptone; a combination of sucrose, dextran, and glutamate; and various combinations of sucrose, glutamate, and potassium phosphates. Suspending media containing glutamate, either alone or in combination with sucrose and either dextran or phosphates, afforded the greatest degree of protection during the freeze-drying process and upon storage. Some possible functions of these additives in preventing injury to the virus during freezing and drying have been suggested.

Effect of K2HPO4, KH2PO4, NaHCO3, and Sodium Ascorbate on Survival of Lactobacillus acidophilus during Freeze-Drying

2012 ◽  
Vol 531 ◽  
pp. 284-287 ◽  
Author(s):  
Zhen Xing Ma ◽  
He Chen ◽  
Guo Wei Shu ◽  
Tao Qin
Keyword(s):  
Survival Rate ◽  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Sodium Ascorbate ◽  
Protective Agent ◽  
Survival Ratio ◽  
Protective Agents ◽  
Viable Cells ◽  
Key Factor

In the present study, the experiments were investigated with the effecting of NaHCO3, KH2PO4, K2HPO4 and Sodium ascorbate on survival of Lactobacillus acidophilus during freeze-drying. Designing different concentrations of protective agents, respectively. The survival ratio of Lactobacillus acidophilus and the number of viable cells will be measured after completing drying. In the process, there were major differences between the different concentrations of protective agents in survival during freeze-drying. As the result, the protective agent was playing a key factor affecting the survival of Lactobacillus acidophilus. At present in the study of the experiments, the highest survival rate was NaHCO3 as the protective agent, and when the protective agent as K2HPO4 the number of viable cells was largest.

scholarly journals Effect of suspending media on freeze-drying and preservation of vaccinia virus

1970 ◽  
Vol 68 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Masatoshi Suzuki
Keyword(s):  
Protective Effect ◽  
Vaccinia Virus ◽  
Carboxymethyl Cellulose ◽  
Freeze Drying ◽  
Chorioallantoic Membrane ◽  
Soluble Starch ◽  
Sodium Glutamate ◽  
Freeze Dried ◽  
Different Temperatures ◽  
Subsequent Storage

SummaryUnpurified and purified smallpox vaccines were prepared from calf dermal pulp, or chorioallantoic membrane (CAM) of hen eggs infected with vaccinia virus, and freeze-dried. The protective effect of various suspending media was investigated both in the course of the freeze-drying and in the period of subsequent storage of the dried product at different temperatures, including 100° C.Single media consisting of either sodium glutamate or peptone were effective in the preservation of both unpurified and purified vaccines prepared from calf dermal pulp or CAM. It was shown that there was an optimal concentration of sodium glutamate for the preservation of the vaccine preparations, especially of the purified vaccine.Combined media, consisting of soluble starch, polyvinylpyrrolidone or sodium carboxymethyl cellulose with sodium glutamate, were effective with the purified vaccine when the concentration of sodium glutamate exceeded the optimum necessary for preservation.

Effect of Four Materials Including Glycine, Arginine, Proline and Sodium Glutamate on Survival of Bifidobacterium bifidum during Freeze-Drying

2013 ◽  
Vol 676 ◽  
pp. 93-96
Author(s):  
Qi Ma ◽  
Tao Qin ◽  
He Chen ◽  
Guo Wei Shu
Keyword(s):  
Survival Rate ◽  
Freeze Drying ◽  
Bifidobacterium Bifidum ◽  
Sodium Glutamate ◽  
Viable Cells ◽  
Before And After

Effect of four materials including glycine, arginine, proline and sodium glutamate on survival of Bifidobacterium bifidum during freeze-drying was studied by measuring the viable counts of before and after freeze-drying. The addition of glycine, arginine, proline and sodium glutamate (%, w/v) were all 1.0%, 2.0%, 3.0%，4.0% and 5.0%. Results were as follows: addition of glycine, arginine, proline and sodium glutamate into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of glycine, arginine, proline and sodium glutamate were 4.0%, 4.0%, 3.0% and 2.0%, the survival of B. bifidum was 87.49%, 84.28%, 67.50% and 69.01%, respectively.

scholarly journals Some factors affecting preservability of freeze-dried bacteria

1961 ◽  
Vol 59 (1) ◽  
pp. 77-91 ◽  
Author(s):  
Yoji Obayashi ◽  
Sunao Ota ◽  
Shiro Arai
Keyword(s):  
Freeze Drying ◽  
Survival Rates ◽  
Heat Stability ◽  
Soluble Starch ◽  
Optimal Concentration ◽  
Sodium Glutamate ◽  
Sublimation Rate ◽  
Factors Affecting ◽  
Freeze Dried ◽  
Significant Difference

1. The use of sodium glutamate as a medium for freeze-drying Lact. bifidus significantly enhanced the stability of the dried product, especially the heat stability.2. The optimal concentration of sodium glutamate, as a drying medium, was found to depend on the bacilliary concentration; the higher the concentration of the bacilliary suspension used the higher was found to be the optimal concentration of the sodium glutamate.3. It was possible to classify various drying media according to the degree of sublimation of the product during the process of freeze-drying. Two types were observed; in the first, which included sodium glutamate and glucose, the sublimation rate diminished with the increasing concentration of the medium, whereas in the second group, which included polyvinyl pyrrolidon K 90 (PVP), soluble starch and dextran, no such diminution was observed when the concentration of the medium was increased.4. By the combination of sodium glutamate or glucose with a medium belonging to the second group sublimation was found to be promoted.5. The stabifity-conferring effect of sodium glutamate on dried Lact. bifidus was markedly enhanced when the glutamate was combined with a drying medium belonging to the second group. This effect is attributed to a desiccation-promoting property of the latter medium.6. Lact. bifidus as a freeze-dried preparation showed a somewhat better survival rate when sealed in air than when sealed in vacuo. In the case of other anaerobic organisms tested there was no significant difference in the survival rates under the two methods of storage.

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