Optimized Platelet-Rich Fibrin With the Low-Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response

2017 ◽  
Vol 88 (1) ◽  
pp. 112-121 ◽  
Author(s):  
Masako Fujioka-Kobayashi ◽  
Richard J. Miron ◽  
Maria Hernandez ◽  
Umadevi Kandalam ◽  
Yufeng Zhang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cellular Response ◽  
Platelet Rich Fibrin ◽  
Low Speed ◽  
Growth Factor Release ◽  
Factor Release

scholarly journals Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

2020 ◽  
Vol 24 (10) ◽  
pp. 3425-3436 ◽  
Author(s):  
Sebastian Blatt ◽  
Valentin Burkhardt ◽  
Peer W. Kämmerer ◽  
Andreas M. Pabst ◽  
Keyvan Sagheb ◽  
...  
Keyword(s):  
Growth Factor ◽  
Immunohistochemical Staining ◽  
Collagen Matrices ◽  
Platelet Rich Fibrin ◽  
Angiogenic Potential ◽  
Branching Points ◽  
Growth Factor Release ◽  
Factor Release

Abstract Objectives Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. Materials and methods Three different CM (mucoderm, jason, collprotect) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. Results Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). Conclusions PRF improved the angiogenesis of CM in vitro and in vivo. Clinical relevance Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.

scholarly journals Hyaluronic Acid Accelerates VEGF and PDGF Release from Advance Platelet Rich Fibrin in Diabetic Foot Ulcer

2021 ◽  
Vol 13 (3) ◽  
pp. 332-6
Author(s):  
Ronald Winardi Kartika ◽  
Idrus Alwi ◽  
Franciscus Dhyanagiri Suyatna ◽  
Ferry Sandra ◽  
Em Yunir ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Growth Factor ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Vascular Endothelial ◽  
Platelet Rich Fibrin ◽  
Growth Factor Release ◽  
Endothelial Growth Factor ◽  
Factor Release

BACKGROUND: Hyaluronic acid (HA) is an essential component of extracellular matrix and mediates signaling in wound healing. HA could induce growth factor release from Advanced Platelet Rich Fibrin (A-PRF), including Vascular Endothelial Growth Factor (VEGF) and Platelet-derived Growth Factor (PDGF). However, concentrations of the released-VEGF and PDGF have not been clearly disclosed. Therefore, current study was conducted to measure the release of these growth factors in HA + A-PRF gel of diabetic foot ulcer (DFU) subjects.METHODS: Twenty DFU subjects were included in the study and treated with A-PRF or HA+A-PRF. A-PRF was derived from autologous peripheral blood and processed with low-speed centrifugation. HA was added with a ratio of 1:0.6. A-PRF or HA + A-PRF was applied topically on DFU. Upper tips of A-PRF or HA + A-PRF gels were collected on day 0, 3 and 7 for measurements of VEGF and PDGF concentrations with Enzyme-linked Immune-sorbent Assay (ELISA) methods.RESULTS: On day-3, both VEGF and PDGF concentrations of HA + A-PRF group were significantly higher than the VEGF (p=0.000) and PDGF (p=0.019) concentrations of A-PRF group. The VEGF and PDGF concentrations were continuously and significantly increased on day-7 of HA + A-PRF group, compared to the VEGF (p=0.000) and PDGF (p=0.004) concentrations of A-PRF group.CONCLUSION: Combination HA+A-PRF induces VEGF and PDGF release from A-PRF. A mixture of A-PRF and HA could be more effective than A-PRF alone for treatment of DFU.KEYWORDS: hyaluronic acid, advanced platelet rich fibrin, PRF, growth factor, VEGF, PDGF, diabetic foot ulcer

Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols

Cytokine ◽  
2017 ◽  
Vol 97 ◽  
pp. 149-155 ◽  
Author(s):  
Román F. Jiménez-Aristizabal ◽  
Catalina López ◽  
María E. Álvarez ◽  
Carlos Giraldo ◽  
Marta Prades ◽  
...  
Keyword(s):  
Growth Factor ◽  
Platelet Rich Fibrin ◽  
Growth Factor Release ◽  
Factor Release ◽  
Fibrin Clots
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