Comparative Study of Chinese Hamster Ovary Cell VersusEscherichia coli-Derived Bone Morphogenetic Protein-2 Using the Critical-Size Supraalveolar Peri-Implant Defect Model

2013 ◽  
Vol 84 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Jaebum Lee ◽  
Eui Nam Lee ◽  
James Yoon ◽  
Sung-Min Chung ◽  
Hari Prasad ◽  
...  
Keyword(s):  
Comparative Study ◽  
Bone Morphogenetic Protein ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Critical Size ◽  
Chinese Hamster ◽  
Bone Morphogenetic Protein 2 ◽  
Ovary Cell ◽  
Defect Model ◽  
Morphogenetic Protein

Expression and Characterization of Bone Morphogenetic Protein-2 in Chinese Hamster Ovary Cells

1992 ◽  
Vol 7 (2) ◽  
pp. 139-150 ◽  
Author(s):  
David I. Israel ◽  
John Nove ◽  
Kelvin M. Kerns ◽  
Ioannis K. Moutsatsos ◽  
Randal J. Kaufman
Keyword(s):  
Bone Morphogenetic Protein ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Bone Morphogenetic Protein 2 ◽  
Ovary Cells ◽  
Morphogenetic Protein

Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model

2021 ◽  
pp. 1-9
Author(s):  
Shintaro Shoji ◽  
Kentaro Uchida ◽  
Ryo Tazawa ◽  
Wataru Saito ◽  
Akiyoshi Kuroda ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Bone Defect ◽  
Bone Repair ◽  
Critical Size ◽  
Bone Morphogenetic Protein 2 ◽  
Micro Computed Tomography ◽  
Defect Model ◽  
Morphogenetic Protein

BACKGROUND: An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHGs). IFHGs may also be ideal carrier materials for bone repair, although their ability to carry bone morphogenetic protein-2 (BMP2) has yet to be examined. OBJECTIVE: We examined the effectiveness of an IFHG made of hyaluronan (IFHG-HA) containing BMP2 for promoting bone formation in a mouse critical size bone defect model. METHODS: C57/BL6J mice received a 2-mm femoral critical-sized bone defect before being randomly assigned to one of the following treatment groups (n = 6): control (no treatment), IFHG-HA only, PBS with BMP2, and IFHG-HA with BMP2. X-ray radiographs were utilized to track new bone formation, and micro-computed tomography and histological examination were performed on new bone formed at the bone defect site two weeks after surgery. RESULTS: Mice treated with PBS with BMP2 and IFHG-HA with BMP2 had greater bone volume (BV) and bone mineral content (BMC) than those receiving control, and successfully achieved consolidation. Mice treated with IFHG-HA with BMP2 had significantly higher BV and BMC than those treated with PBS with BMP2. CONCLUSIONS: IFHG-HA may be an effective carrier for BMP2 to enable delivery for bone defect repair.

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