Role of p38 Mitogen-Activated Protein Kinase Pathway inPorphyromonas gingivalisLipopolysaccharide–Induced VCAM-1 Expression in Human Aortic Endothelial Cells

2012 ◽  
Vol 83 (7) ◽  
pp. 955-962 ◽  
Author(s):  
Bin Liu ◽  
Lan Cheng ◽  
DaLi Liu ◽  
Jia Wang ◽  
XiuLi Zhang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Protein Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Aortic Endothelial Cells ◽  
Mitogen Activated Protein ◽  
Human Aortic Endothelial Cells ◽  
Kinase Pathway ◽  
Aortic Endothelial

scholarly journals Glyoxal Damages Human Aortic Endothelial Cells by Perturbing the Glutathione, Mitochondrial Membrane Potential, and Mitogen-Activated Protein Kinase Pathways

2021 ◽  
Author(s):  
Ming-Zhang Xie ◽  
Chun Guo ◽  
Jia-Qi Dong. BA ◽  
Jie Zhang ◽  
Ke-Tao Sun ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Membrane Potential ◽  
Protein Kinase ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Mitogen Activated Protein Kinase ◽  
Aortic Endothelial Cells ◽  
Mitogen Activated Protein ◽  
Human Aortic Endothelial Cells ◽  
Quantitative Fluorescence

Abstract Background: Exposure to glyoxal, the smallest dialdehyde, is associated with several diseases; humans are routinely exposed to glyoxal because of its ubiquitous presence in foods and the environment. The aim of this study was to examine the damage caused by glyoxal in human aortic endothelial cells. Methods: Cell survival assays and quantitative fluorescence assays were performed to measure DNA damage; oxidative stress was detected by colorimetric assays and quantitative fluorescence, and the mitogen-activated protein kinase pathways were assessed using western blotting. Results: Exposure to glyoxal was found to be linked to abnormal glutathione activity, the collapse of mitochondrial membrane potential, and the activation of mitogen-activated protein kinase pathways. However, DNA damage and thioredoxin oxidation were not induced by dialdehydes. Conclusions: Intracellular glutathione, members of the mitogen-activated protein kinase pathways, and the mitochondrial membrane potential are all critical targets of glyoxal. These findings provide novel insights into the molecular mechanisms perturbed by glyoxal and may facilitate the development of new therapeutics and diagnostic markers for cardiovascular diseases.

The Role of Rho/Rho-Kinase Pathway in the Expression of ICAM-1 by Linoleic Acid in Human Aortic Endothelial Cells

Inflammation ◽  
2011 ◽  
Vol 35 (3) ◽  
pp. 1041-1048 ◽  
Author(s):  
Chang Hee Jung ◽  
Woo Je Lee ◽  
Jenie Yoonoo Hwang ◽  
So Mi Seol ◽  
Yun Mi Kim ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Linoleic Acid ◽  
Rho Kinase ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Kinase Pathway ◽  
Aortic Endothelial

scholarly journals Glyoxal damages human aortic endothelial cells by perturbing the glutathione, mitochondrial membrane potential, and mitogen-activated protein kinase pathways

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ming-Zhang Xie ◽  
Chun Guo ◽  
Jia-Qi Dong ◽  
Jie Zhang ◽  
Ke-Tao Sun ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Membrane Potential ◽  
Protein Kinase ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Mitogen Activated Protein Kinase ◽  
Aortic Endothelial Cells ◽  
Mitogen Activated Protein ◽  
Human Aortic Endothelial Cells ◽  
Quantitative Fluorescence

Abstract Background Exposure to glyoxal, the smallest dialdehyde, is associated with several diseases; humans are routinely exposed to glyoxal because of its ubiquitous presence in foods and the environment. The aim of this study was to examine the damage caused by glyoxal in human aortic endothelial cells. Methods Cell survival assays and quantitative fluorescence assays were performed to measure DNA damage; oxidative stress was detected by colorimetric assays and quantitative fluorescence, and the mitogen-activated protein kinase pathways were assessed using western blotting. Results Exposure to glyoxal was found to be linked to abnormal glutathione activity, the collapse of mitochondrial membrane potential, and the activation of mitogen-activated protein kinase pathways. However, DNA damage and thioredoxin oxidation were not induced by dialdehydes. Conclusions Intracellular glutathione, members of the mitogen-activated protein kinase pathways, and the mitochondrial membrane potential are all critical targets of glyoxal. These findings provide novel insights into the molecular mechanisms perturbed by glyoxal, and may facilitate the development of new therapeutics and diagnostic markers for cardiovascular diseases.

Sign in / Sign up

Export Citation Format

Share Document