The Impact of Smoking Status on Antioxidant Enzyme Activity and Malondialdehyde Levels in Chronic Periodontitis

2011 ◽  
Vol 82 (9) ◽  
pp. 1320-1328 ◽  
Author(s):  
Mine Öztürk Tonguç ◽  
Önder Öztürk ◽  
Recep Sütçü ◽  
Betül Mermi Ceyhan ◽  
Gizem Kılınç ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Chronic Periodontitis ◽  
Smoking Status ◽  
Antioxidant Enzyme Activity ◽  
The Impact

scholarly journals The Impact of a Diet Containing Sucrose and Systematically Repeated Starvation on the Oxidative Status of the Uterus and Ovary of Rats

Nutrients ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1544 ◽  
Author(s):  
Joanna Sadowska ◽  
Wioleta Dudzińska ◽  
Ewa Skotnicka ◽  
Katarzyna Sielatycka ◽  
Izabela Daniel
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Oxidative Status ◽  
Antioxidant Enzyme Activity ◽  
Female Rats ◽  
Egg Cell ◽  
Starvation Period ◽  
Fertility Disorders ◽  
The Impact ◽  
Cell Implantation

The effect of a sucrose diet and repeated one-day starvation on oxidative status in the ovary and uterus is still unknown. Our analysis focused on carbohydrate-lipid metabolism parameters and the changes in red blood cells, ovary and uterus superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities and malonylodialdehyde (MDA) concentration in rats fed with a diet containing 16% of sucrose and subjected to systematic one-day starvation when using such a diet. It was found that a diet with 16% sucrose contributed to the increase of antioxidant enzyme activity in the blood (GPx and CAT) and uterus (SOD), without changes in MDA concentrations, which indicates an increase in reactive oxygen species (ROS) concentration in these tissues, being balanced by an increase in antioxidant enzyme activity. The introduction of a regular one-day starvation period into the diet intensified oxidative stress and led to a redox imbalance in the reproductive tissues of female rats. This was manifested by higher GPx activity, lower CAT activity and higher MDA concentration in the uterus and lower GPx and CAT activities and lower MDA concentration in the ovaries. The observed changes may be the cause of fertility disorders and possible problems with fertilised egg cell implantation into the uterine tissue.

Influence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle

1994 ◽  
Vol 266 (2) ◽  
pp. R375-R380 ◽  
Author(s):  
S. K. Powers ◽  
D. Criswell ◽  
J. Lawler ◽  
L. L. Ji ◽  
D. Martin ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Exercise Training ◽  
Antioxidant Enzyme ◽  
Exercise Intensity ◽  
Fiber Type ◽  
Exercise Duration ◽  
Antioxidant Enzyme Activity ◽  
Induced Changes ◽  
The Impact

These experiments examined the influence of exercise intensity and duration on antioxidant enzyme activity in locomotor muscles differing in fiber type composition. Nine groups of female Sprague-Dawley rats (age 120 days) exercised 4 days/wk on a motor-driven treadmill for 10 wk. The impact of three levels of exercise intensity (low, moderate, and high: approximately 55, approximately 65, and approximately 75% of maximal oxygen consumption, respectively) and exercise duration (30, 60, and 90 min/day) was assessed. Sedentary animals served as controls. Oxidative capacity in the soleus and white and red gastrocnemius was assessed by measurement of citrate synthase (CS) activity, and antioxidant capacity was evaluated by assay of total superoxide dismutase, catalase, and total glutathione peroxidase (GPX) activities. In all muscles, CS activity increased as a function of exercise duration. Furthermore, in the soleus and white gastrocnemius, the magnitude of the training-induced increase in CS activity was directly related to exercise intensity. In contrast, the peak increase in CS activity in the red gastrocnemius was relatively independent of exercise intensity. Catalase activity was not increased (P > 0.05) in any muscle with training. Training-induced changes in superoxide dismutase and GPX activities were muscle specific; specifically, exercise training significantly (P < 0.05) increased superoxide dismutase activity in the soleus as a function of exercise duration up to 60 min/day. Conversely, training-induced significant (P < 0.05) increases in GPX activity occurred in red gastrocnemius only; the magnitude of the GPX increase was directly related to exercise duration but relatively independent of intensity. These data demonstrate that exercise training-induced changes in muscle antioxidant enzymes are muscle specific.

scholarly journals Modified Atmospheric Packaging of Fresh-Cut Amaranth (Amaranthus tricolor L.) for Extending Shelf Life

Agriculture ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1016
Author(s):  
Siyuan Jin ◽  
Zhaoyang Ding ◽  
Jing Xie
Keyword(s):  
Enzyme Activity ◽  
Shelf Life ◽  
Antioxidant Enzyme ◽  
Ascorbic Acid Content ◽  
Antioxidant Enzyme Activity ◽  
Physiological And Biochemical Characteristics ◽  
Modified Atmospheric Packaging ◽  
Fresh Cut ◽  
The Impact ◽  
Physiological And Biochemical

Fresh-cut vegetables are prone to microbiological contamination and oxygenation during handling and storage. In this study, fresh-cut amaranth was subjected to various gas ratios (5–15% O2, 5–15% CO2, 80% N2) for 12 days. Chlorophyll content, ascorbic acid content, antioxidant enzyme activity, microbial population, and physiological and biochemical indicators were measured to evaluate the impact of atmospheric packaging. Suitable atmospheric packaging could slow the respiration of amaranth, delay the decline in physiological and biochemical characteristics, maintain the antioxidant enzyme activity, promote the sensorics, and prolong the shelf life by 2 days. According to the analysis of the results, modified atmospheric packaging (10% O2, 10% CO2, 80% N2) retarded the decline in fresh-cut amaranth quality, provided effective antioxidative browning, and inhibited Pseudomonas fluorescens development.

Nitric Oxide Enhances Salt Tolerance through Regulating Antioxidant Enzyme Activity and Nutrient Uptake in Pea

2020 ◽  
Author(s):  
Esin Dadasoglu ◽  
Melek Ekinci ◽  
Raziye Kul ◽  
Mostafakamal Shams ◽  
Metin Turan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Enzyme Activity ◽  
Salt Stress ◽  
Nutrient Uptake ◽  
Antioxidant Enzyme ◽  
Crop Production ◽  
Electrical Conductance ◽  
Antioxidant Enzyme Activity ◽  
Stress Factors ◽  
The Impact

Background: Salinity is one of the environmental stress factors that restrict the crop production by endangering agricultural areas. Nitric oxide (NO) protects plants from damage caused by oxidative stress conditions in various biological ways. Methods: In this greenhouse investigation during 2018, pea plants were irrigated with three levels of NaCl (0, 50 and 100 mM) solutions. NO solutions were prepared with three different doses (0, 75 and 100 µM SNP). These solutions were applied to the seeds before sowing and then to the leaves of the pea cultivars. The study was conducted to analyze the impact of NO on growth, malondialdehyde (MDA), hydrogen peroxide (H2O2), antioxidant enzyme activity and nutrient uptake in two pea cultivars under salinity conditions. Result: Salinity reduced fresh-dry weight, relative water content (RWC), and chlorophyll a and b content of pea. However, NO enhanced these parameters under salt stress. Salinity increased tissue electrical conductance (TEC), H2O2 and MDA content, which were decreased by combined application of NaCl and NO. Salinity caused an increase in antioxidant enzyme activity in pea and NO made a significant improvement in their activities under salinity conditions. Salinity treatments decreased the ratio of K+/Na+ and Ca2+/Na+ in both cultivars, and application of NO elevated them as compared to the control under salt stress. In conclude, exogenous NO treatment could help pea to tolerate salinity stress by increasing the chlorophyll content and regulating antioxidant enzyme activity and nutrient uptake.

Impaired terpenoid backbone biosynthesis reduces saponin accumulation in Panax notoginseng under Cd stress

2019 ◽  
Vol 46 (1) ◽  
pp. 56 ◽  
Author(s):  
Peiran Liao ◽  
Yue Shi ◽  
Ziwei Li ◽  
Qi Chen ◽  
Tian-Rui Xu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Panax Notoginseng ◽  
Antioxidant Enzyme Activity ◽  
Sequencing Analysis ◽  
Rna Seq ◽  
Cd Stress ◽  
Real Time Quantitative Pcr ◽  
Regulation Mechanisms ◽  
The Impact

Panax notoginseng saponins (PNS) are major secondary metabolite of Panax notoginseng (Burk.) F.H. Chen. Previous studies identified that P. notoginseng planting soil usually with high content of Cd. However, the effects of Cd stress on the accumulation of PNS and the corresponding regulation mechanisms have yet to be reported. In the present study, the impact of Cd stress on the PNS accumulation of P. notoginseng was studied in pot culture experiments. The effect of Cd stress on antioxidant enzyme activity was studied using hydroponics. In addition, transcriptase sequencing analysis was used to study the effect of Cd stress on the expression of PNS metabolism transcripts in hydroponic experiments. Cd treatments significantly decreased the accumulation of PNS in the rhizome and main root. The sensitive concentration of antioxidant enzyme activity for both leaf and stem was 2.5μM, whereas the sensitive concentration for the root was 5.0μM. Transcriptome analysis showed that 5132 genes (2930 up- and 2202 downregulated) were regulated by 5.0μM Cd stress in the root of P. notoginseng. Among them, six upregulated differentially expressed genes (DEGs) were related to the methylerythritol 4-phosphate (MEP) pathway, whereas three of the downregulated DEGs were mevalonate kinase (MVK), phosphomevalonate kinase (PMK), and geranylgeranyl diphosphate synthase (type II, GGPS). Of the 15 transcripts selected for real-time quantitative-PCR, 13 were expressed in the same manner as identified using RNA-seq. In conclusion, Cd stress inhibited the accumulation of PNS in the root of P. notoginseng by reducing the expression of MVK, PMK, and GGPS in the terpenoid backbone biosynthesis pathway, and also caused by the removal of reactive oxygen species.

Antioxidant Enzyme Levels Inversely Covary with Hearing Loss After Amikacin Treatment

2003 ◽  
Vol 14 (03) ◽  
pp. 134-143 ◽  
Author(s):  
James J. Klemens ◽  
Robert P. Meech ◽  
Larry F. Hughes ◽  
Satu Somani ◽  
Kathleen C.M. Campbell
Keyword(s):  
Hearing Loss ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Guinea Pigs ◽  
Auditory Brainstem ◽  
Antioxidant Enzyme Activity ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Brainstem Response ◽  
The Difference

This study's purpose was to determine if a correlation exists between cochlear antioxidant activity changes and auditory function after induction of aminoglycoside (AG) ototoxicity. Two groups of five 250-350 g albino guinea pigs served as subjects. For 28 days, albino guinea pigs were administered either 200 mg/kg/day amikacin, or saline subcutaneously. Auditory brainstem response testing was performed prior to the first injection and again before sacrifice, 28 days later. Cochleae were harvested and superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase activities and malondialdehyde levels were measured. All antioxidant enzymes had significantly lower activity in the amikacin group (p ≤ 0.05) than in the control group. The difference in cochlear antioxidant enzyme activity between groups inversely correlated significantly with the change in ABR thresholds. The greatest correlation was for the high frequencies, which are most affected by aminoglycosides. This study demonstrates that antioxidant enzyme activity and amikacin-induced hearing loss significantly covary.

Sign in / Sign up

Export Citation Format

Share Document