Superior Effect of MD05, Beta-Tricalcium Phosphate Coated With Recombinant Human Growth/Differentiation Factor-5, Compared to Conventional Bone Substitutes in the Rat Calvarial Defect Model

2006 ◽  
Vol 77 (9) ◽  
pp. 1582-1590 ◽  
Author(s):  
Sylke Poehling ◽  
Susanne D. Pippig ◽  
Klaus Hellerbrand ◽  
Michael Siedler ◽  
Andreas Schütz ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Human Growth ◽  
Bone Substitutes ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
Differentiation Factor ◽  
Superior Effect ◽  
Rat Calvarial Defect ◽  
Growth Differentiation Factor 5

Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

2019 ◽  
Vol 7 (20) ◽  
pp. 3250-3259 ◽  
Author(s):  
Yali Miao ◽  
Yunhua Chen ◽  
Xiao Liu ◽  
Jingjing Diao ◽  
Naru Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
3D Printed ◽  
Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate

2010 ◽  
Vol 112 (3) ◽  
pp. 270-277 ◽  
Author(s):  
Nobuaki Shirasu ◽  
Takaaki Ueno ◽  
Yasuhisa Hirata ◽  
Azumi Hirata ◽  
Toshimasa Kagawa ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Tricalcium Phosphate ◽  
Autogenous Bone ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
Rat Calvarial Defect

scholarly journals Fabrication and Evaluation of Porous Beta-Tricalcium Phosphate/Hydroxyapatite (60/40) Composite as a Bone Graft Extender Using Rat Calvarial Bone Defect Model

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Jae Hyup Lee ◽  
Mi Young Ryu ◽  
Hae-Ri Baek ◽  
Kyung Mee Lee ◽  
Jun-Hyuk Seo ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Micro Ct ◽  
Porous Substrate ◽  
New Bone Formation ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
Bone Graft Extender

Beta-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) are widely used as bone graft extenders due to their osteoconductivity and high bioactivity. This study aims to evaluate the possibility of using porous substrate with composite ceramics (β-TCP: HA = 60% : 40%, 60TCP40HA) as a bone graft extender and comparing it with Bio-Oss. Interconnectivity and macroporosity ofβ-TCP porous substrate were 99.9% and 83%, respectively, and the macro-porosity of packed granule after crushing was 69%. Calvarial defect model with 8 mm diameter was generated with male Sprague-Dawley rats and 60TCP40HA was implanted. Bio-Oss was implanted for a control group and micro-CT and histology were performed at 4 and 8 weeks after implantation. The 60TCP40HA group showed better new bone formation than the Bio-Oss group and the bone formation at central area of bone defect was increased at 8 weeks in micro-CT and histology. The percent bone volume and trabecular number of the 60TCP40HA group were significantly higher than those of Bio-Oss group. This study confirms the usefulness of the porous 60TCP40HA composite as a bone graft extender by showing increased new bone formation in the calvarial defect model and improved bone formation both quantitatively and qualitatively when compared to Bio-Oss.

scholarly journals Controlled Release of Granulocyte Colony-Stimulating Factor Enhances Osteoconductive and Biodegradable Properties of Beta-Tricalcium Phosphate in a Rat Calvarial Defect Model

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Tomohiro Minagawa ◽  
Yasuhiko Tabata ◽  
Akihiko Oyama ◽  
Hiroshi Furukawa ◽  
Takeshi Yamao ◽  
...  
Keyword(s):  
Controlled Release ◽  
Calvarial Defect ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
Controlled Release System ◽  
Biodegradable Properties ◽  
Rat Calvarial Defect ◽  
Stimulating Factor

Autologous bone grafts remain the gold standard for the treatment of congenital craniofacial disorders; however, there are potential problems including donor site morbidity and limitations to the amount of bone that can be harvested. Recent studies suggest that granulocyte colony-stimulating factor (G-CSF) promotes fracture healing or osteogenesis. The purpose of the present study was to investigate whether topically applied G-CSF can stimulate the osteoconductive properties of beta-tricalcium phosphate (β-TCP) in a rat calvarial defect model. A total of 27 calvarial defects 5 mm in diameter were randomly divided into nine groups, which were treated with various combinations of aβ-TCP disc and G-CSF in solution form or controlled release system using gelatin hydrogel. Histologic and histomorphometric analyses were performed at eight weeks postoperatively. The controlled release of low-dose (1 μg and 5 μg) G-CSF significantly enhanced new bone formation when combined with aβ-TCP disc. Moreover, administration of 5 μg G-CSF using a controlled release system significantly promoted the biodegradable properties ofβ-TCP. In conclusion, the controlled release of 5 μg G-CSF significantly enhanced the osteoconductive and biodegradable properties ofβ-TCP. The combination of G-CSF slow-release andβ-TCP is a novel and promising approach for treating pediatric craniofacial bone defects.

scholarly journals Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model

2021 ◽  
Vol 11 (24) ◽  
pp. 11941
Author(s):  
Nobuhito Tsumano ◽  
Hirohito Kubo ◽  
Rie Imataki ◽  
Yoshitomo Honda ◽  
Yoshiya Hashimoto ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Electrostatic Interactions ◽  
Bone Defects ◽  
Gelatin Sponge ◽  
Calvarial Defect ◽  
Micro Computed Tomography ◽  
Defect Model ◽  
Rat Calvarial Defect ◽  
Dfat Cells

Mechanical and resorbable scaffolds are in high demand for stem cell-based regenerative medicine, to treat refractory bone defects in craniofacial abnormalities and injuries. Multipotent progenitor cells, such as dedifferentiated fat (DFAT) cells, are prospective sources for regenerative therapies. Herein, we aimed to demonstrate that a composite gelatin sponge (α-TCP/GS) of alfa-tricalcium phosphate (α-TCP) mixed with gelatin scaffolds (GS), with/without DFATs, induced bone regeneration in a rat calvarial defect model in vivo. α-TCP/GS was prepared by mixing α-TCP and 2% GS using vacuum-heated methods. α-TCP/GS samples with/without DFATs were transplanted into the model. After 4 weeks of implantation, the samples were subjected to micro-computed tomography (μ-CT) and histological analysis. α-TCP/GS possessed adequate mechanical strength; α-TCP did not convert to hydroxyapatite upon contact with water, as determined by X-ray diffraction. Moreover, stable α-TCP/GS was formed by electrostatic interactions, and verified based on the infrared peak shifts. μ-CT analyses showed that bone formation was higher in the α-TCP/GS+ DFAT group than in the α-TCP/GS group. Therefore, the implantation of α-TCP/GS comprising DFAT cells enhanced bone regeneration and vascularization, demonstrating the potential for healing critical-sized bone defects.

Sign in / Sign up

Export Citation Format

Share Document