scholarly journals Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?

2017 ◽  
Vol 14 (1) ◽  
pp. 22-38 ◽  
Author(s):  
Daniel Brandhorst ◽  
Heide Brandhorst ◽  
Paul R.V. Johnson
Keyword(s):  
Human Islet ◽  
Islet Isolation ◽  
Enzyme Development

scholarly journals Evaluation of Viable β-Cell Mass is Useful for Selecting Collagenase for Human Islet Isolation: Comparison of Collagenase NB1 and Liberase HI

2012 ◽  
Vol 21 (1) ◽  
pp. 39-47 ◽  
Author(s):  
R. Misawa ◽  
C. Ricordi ◽  
A. Miki ◽  
S. Barker ◽  
R. D. Molano ◽  
...  
Keyword(s):  
Cell Mass ◽  
Human Islet ◽  
Islet Isolation ◽  
Β Cell

Efficacy of Human Islet Isolation From the Tail Section of the Pancreas for the Possibility of Living Donor Islet Transplantation

2004 ◽  
Vol 78 (6) ◽  
pp. 839-843 ◽  
Author(s):  
Shinichi Matsumoto ◽  
Koichi Tanaka ◽  
D Michael Strong ◽  
Jo Anna Reems
Keyword(s):  
Islet Transplantation ◽  
Living Donor ◽  
Human Islet ◽  
Islet Isolation

scholarly journals Clostripain, the Missing Link in the Enzyme Blend for Efficient Human Islet Isolation

2015 ◽  
Vol 1 (5) ◽  
pp. 1-6 ◽  
Author(s):  
Magnus Ståhle ◽  
Aksel Foss ◽  
Bengt Gustafsson ◽  
Marko Lempinen ◽  
Torbjörn Lundgren ◽  
...  
Keyword(s):  
Human Islet ◽  
Islet Isolation ◽  
Missing Link

scholarly journals Human Islet Isolation Media Preparation v1 (protocols.io.sfyebpw)

protocols.io ◽  
2018 ◽  
Author(s):  
James Lyon ◽  
Patrick MacDonald ◽  
Jocelyn Manning
Keyword(s):  
Human Islet ◽  
Islet Isolation ◽  
Isolation Media

scholarly journals Research-Focused Isolation of Human Islets From Donors With and Without Diabetes at the Alberta Diabetes Institute IsletCore

Endocrinology ◽  
2015 ◽  
Vol 157 (2) ◽  
pp. 560-569 ◽  
Author(s):  
James Lyon ◽  
Jocelyn E. Manning Fox ◽  
Aliya F. Spigelman ◽  
Ryekjang Kim ◽  
Nancy Smith ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Glycated Hemoglobin ◽  
Organ Procurement ◽  
Human Islets ◽  
Human Islet ◽  
Islet Function ◽  
Small Contribution ◽  
Islet Isolation ◽  
Diabetes Status

Abstract Recent years have seen an increased focus on human islet biology, and exciting findings in the stem cell and genomic arenas highlight the need to define the key features of mature human islets and β-cells. Donor and organ procurement parameters impact human islet yield, although for research purposes islet yield may be secondary in importance to islet function. We examined the feasibility of a research-only human islet isolation, distribution, and biobanking program and whether key criteria such as cold ischemia time (CIT) and metabolic status may be relaxed and still allow successful research-focused isolations, including from donors with type 1 diabetes and type 2 diabetes. Through 142 isolations over approximately 5 years, we confirm that CIT and glycated hemoglobin each have a weak negative impacts on isolation purity and yield, and extending CIT beyond the typical clinical isolation cutoff of 12 hours (to ≥ 18 h) had only a modest impact on islet function. Age and glycated hemoglobin/type 2 diabetes status negatively impacted secretory function; however, these and other biological (sex, body mass index) and procurement/isolation variables (CIT, time in culture) appear to make only a small contribution to the heterogeneity of human islet function. This work demonstrates the feasibility of extending acceptable CIT for research-focused human islet isolation and highlights the biological variation in function of human islets from donors with and without diabetes.

A NEW ENZYME PREPRARATION FOR HUMAN ISLET ISOLATION

2003 ◽  
Vol 76 (Supplement) ◽  
pp. S59
Author(s):  
P Bucher ◽  
Z Mathe ◽  
D Bosco ◽  
A. Andres ◽  
M. Kurfuerst ◽  
...  
Keyword(s):  
Human Islet ◽  
Islet Isolation

THE INFLUENCE OF PANCREAS PRESERVATION ON HUMAN ISLET ISOLATION OUTCOMES: IMPACT OF THE TWO-LAYER METHOD

2004 ◽  
Vol 78 ◽  
pp. 352
Author(s):  
T Tsujimura ◽  
Y Kuroda ◽  
J Avila ◽  
T Kin ◽  
A M.J. Shapiro ◽  
...  
Keyword(s):  
Human Islet ◽  
Islet Isolation ◽  
Layer Method ◽  
Pancreas Preservation

scholarly journals Successful Human Islet Isolation Utilizing Recombinant Collagenase

Diabetes ◽  
2003 ◽  
Vol 52 (5) ◽  
pp. 1143-1146 ◽  
Author(s):  
H. Brandhorst ◽  
D. Brandhorst ◽  
F. Hesse ◽  
D. Ambrosius ◽  
M. Brendel ◽  
...  
Keyword(s):  
Human Islet ◽  
Islet Isolation

Donor and Isolation Variables Predicting Human Islet Isolation Success

2008 ◽  
Vol 85 (7) ◽  
pp. 950-955 ◽  
Author(s):  
Stephen C. Hanley ◽  
Steven Paraskevas ◽  
Lawrence Rosenberg
Keyword(s):  
Human Islet ◽  
Islet Isolation

scholarly journals Factors That Affect Human Islet Isolation

2008 ◽  
Vol 40 (2) ◽  
pp. 343-345 ◽  
Author(s):  
Y. Sakuma ◽  
C. Ricordi ◽  
A. Miki ◽  
T. Yamamoto ◽  
A. Pileggi ◽  
...  
Keyword(s):  
Human Islet ◽  
Islet Isolation
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