Determination of fish host use by wild populations of rare freshwater mussels using a molecular identification key to identify glochidia

2008 ◽  
Vol 27 (1) ◽  
pp. 150-160 ◽  
Author(s):  
Stephen C. Kneeland ◽  
Judith M. Rhymer
Keyword(s):  
Molecular Identification ◽  
Freshwater Mussels ◽  
Fish Host ◽  
Identification Key ◽  
Host Use ◽  
Wild Populations

scholarly journals Determination of the species among mitis group of genus Streptococus using a molecular identification key

2012 ◽  
pp. 17-24 ◽  
Author(s):  
Istvan Kanjo
Keyword(s):  
Ribosomal Protein ◽  
Molecular Identification ◽  
Gene Order ◽  
Trna Synthetase ◽  
Identification Key ◽  
Conserved Synteny ◽  
Synteny Blocks ◽  
Gene Order Conservation ◽  
Dipeptidyl Aminopeptidase

We analyzed the gene order conservation among the species of genus Streptococcus and based on this we developed a molecular identification key. The identification key was not made up based on the genes? amino acid or nucleotide sequences but on the gene order of certain genome parts, and in this order the genes were marked with their symbols. First, we created a key to determine the groups within the genus Streptococcus using conserved synteny blocks in genomes. According to this, within those species that have a fully known genome sequence, the mitis group can be divided into two subgroups: mitis I where (S. pneumoniae, S mitis and S. oralis) and mitis II (S. gordonii, S. sanguinis and S. parasanguinis). Then we made an identification key to determine the species within the mitis subgroups. For determination of the species we chose genome parts which contain such a gene that is found in all species but the genome parts around this special gene are different in various species. Three genome parts were sufficient to determine the species correctly. These sequences were around the following genes: Xaa-pro dipeptidyl-peptidase (x-prolyl-dipeptidyl aminopeptidase) - pepX, a leucyl-tRNA synthetase - leuS and a 50S ribosomal protein L13 and 30S ribosomal protein S9 - rplM and rpsI (these are always found beside one another).

scholarly journals Analysis of Genetic Variability in Farmed and Wild Populations of Raccoon Dog (Nyctereutes Procyonoides) Using Microsatellite Sequences

2015 ◽  
Vol 15 (4) ◽  
pp. 889-901 ◽  
Author(s):  
Kornel Kasperek ◽  
Beata Horecka ◽  
Andrzej Jakubczak ◽  
Brygida Ślaska ◽  
Magdalena Gryzińska ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Microsatellite Loci ◽  
Nyctereutes Procyonoides ◽  
Wild Animals ◽  
Raccoon Dog ◽  
Wild Populations ◽  
Kaliningrad Region ◽  
Raccoon Dogs

Abstract The aim of this study was to detect possible differences between farmed and wild-living raccoon dogs. Analysis of polymorphism in 15 microsatellite sequences led to the conclusion that raccoon dogs raised on Polish farms and wild raccoon dogs living in Poland are two genetically distinct groups of animals. Wild Polish raccoon dogs are genetically more similar to the population of wild animals from the Kaliningrad Region than to farmed animals. The analysis of microsatellite loci showed clear genetic differences between farmed and wild-living populations of raccoon dog, despite only 50 years of isolation of the two groups of animals. The farmed population was characterized by higher genetic variation than the wild-living population. On the basis of the analyses three microsatellite loci (INU014, Ren13J22 and Ren41D20) were proposed for determination of the origin of animals that have escaped from farms.

scholarly journals Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen

2009 ◽  
Vol 7 (4) ◽  
pp. 629-634 ◽  
Author(s):  
Josiane Ribolli ◽  
Evoy Zaniboni-Filho
Keyword(s):  
Genetic Variability ◽  
Natural Environments ◽  
Wild Populations ◽  
Genetic Composition ◽  
River Damming ◽  
Rhamdia Quelen ◽  
Individual Contributions ◽  
The Individual ◽  
Dna Microsatellite

Supplementary stocking of fish in natural environments is a way to mitigate or compensate for the changes imposed on wild populations by river damming. Since little is known about the genetic composition of the supplementary stocks obtained by pooled-milt fertilization, the aim of this study was to determine the individual contributions of male jundiá Rhamdia quelen to offspring. Sperm from four males were mixed using equal volume of sperm from each of the males to fertilize eggs from only one female, kept in three blend with six males and three females. The proportions of larvae sired by the different males were quantified using five polymorphic DNA microsatellite loci. Analysis of these loci allowed paternal determination of 84% of the progeny, at a 0.972 combined exclusion probability. Broodstock milt had good fertilizing capacity when used alone, but when pooled the fertilizing capacities, its fertilizing possibility varied from 4 to 65%. Results show that milt pools favor gametes of some males over others, thus reducing the progeny's genetic variability.

scholarly journals Prevalence and Antimicrobial Resistance of ThermophilicCampylobacterIsolated from Chicken in Côte d’Ivoire

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Goualié Gblossi Bernadette ◽  
Akpa Eric Essoh ◽  
Kakou-N'Gazoa Elise Solange ◽  
Guessennd Natalie ◽  
Bakayoko Souleymane ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Identification ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Poultry Meat ◽  
Campylobacter Coli ◽  
Cote D’Ivoire ◽  
Filtration Method ◽  
Côte D'ivoire

ThermophilicCampylobactersare major causes of gastroenteritis in human. The main risk factor of infection is consumption of contaminated or by cross-contaminated poultry meat. In Côte d’Ivoire, gastroenteritis is usually observed but no case of human campylobacteriosis has been formally reported to date. The aims of this study were to determine prevalence and antimicrobial resistance ofCampylobacter jejuniandCampylobacter coliisolated from chickens ceaca in commercial slaughter in Abidjan. Between May and November 2009, one hundred and nineteen (119) chicken caeca samples were collected and analyzed by passive filtration method followed by molecular identification (PCR). From these 119 samples, 76 (63.8%) were positive toCampylobactertests. Among the positive colonies, 51.3% wereC. jejuniand 48.7% wereC. coli. Of the 39C. jejuniisolates, 79.5%, 38.5%, 17.9%, 10.3%, and 7.7% were, respectively, resistant, to nalidixic acid, ciprofloxacin, amoxicillin, erythromycin, and gentamicin. Among the 37 isolates ofC. coli, 78.4%, 43.2%, 13.5%, 8.1%, and 0% were resistant, respectively, to the same antibiotics. In conclusion, we reported in this study the presence of highCampylobactercontamination of the studied chickens. Molecular identification of the bacteria was performed and determination of high resistance to antimicrobials of the fluoroquinolone family was revealed.

Molecular Determination of Mixed Infections of Dermatophytes and Nondermatophyte Molds in Individuals with Onychomycosis

2014 ◽  
Vol 104 (4) ◽  
pp. 330-336 ◽  
Author(s):  
Aditya K. Gupta ◽  
Kerry-Ann Nakrieko
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Identification ◽  
Mixed Infection ◽  
Traditional Culture ◽  
Mixed Infections ◽  
Molecular Analyses ◽  
Scopulariopsis Brevicaulis ◽  
Infecting Organisms ◽  
Molecular Determination

Background Reports of mixed infections with nondermatophyte molds (NDMs) and dermatophytes in onychomycosis are rare, possibly owing to the inhibition of NDM growth during traditional culture. We sought to determine the prevalence of mixed infections in onychomycosis using molecular identification. Methods Molecular analyses were used to identify infecting organisms directly from at least two serial great toenail samples from each of the 44 patients. Results Mixed infections were present in 41% of the patients (18 of 44). A single coinfecting NDM was the most common mixed infection and was detected in 34% of patients with onychomycosis (15 of 44), with Fusarium oxysporum present in 14% (6 of 44), Scopulariopsis brevicaulis in 9% (4 of 44), Acremonium spp in 2% (1 of 44), Aspergillus spp in 4.5% (2 of 44), and Scytalidium spp in 4.5% (2 of 44). Mixed infections with two NDMs were found in 7% of patients (3 of 44). Conclusions Mixed onychomycosis infections may be more prevalent than previously reported.

Molecular Identification Key for Pest Species of Scirtothrips (Thysanoptera: Thripidae)

2006 ◽  
Vol 99 (5) ◽  
pp. 1813-1819 ◽  
Author(s):  
Paul F. Rugman-Jones ◽  
Mark S. Hoddle ◽  
Laurence A. Mound ◽  
Richard Stouthamer
Keyword(s):  
Molecular Identification ◽  
Identification Key ◽  
Pest Species
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