IMPACT OF PERFLUOROOCTANOIC ACID ON FATHEAD MINNOW (PIMEPHALES PROMELAS) FATTY ACYL-COA OXIDASE ACTIVITY, CIRCULATING STEROIDS, AND REPRODUCTION IN OUTDOOR MICROCOSMS

2004 ◽  
Vol 23 (8) ◽  
pp. 1912 ◽  
Author(s):  
Ken D. Oakes ◽  
Paul K. Sibley ◽  
Keith R. Solomon ◽  
Scott A. Mabury ◽  
Glen J. Van Der Kraak
Keyword(s):  
Oxidase Activity ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Perfluorooctanoic Acid ◽  
Fatty Acyl ◽  
Acyl Coa Oxidase

scholarly journals Detection of peroxisomal fatty acyl-coenzyme A oxidase activity

1979 ◽  
Vol 182 (3) ◽  
pp. 779-788 ◽  
Author(s):  
N C Inestrosa ◽  
M Bronfman ◽  
F Leighton
Keyword(s):  
Fatty Acid ◽  
Oxidase Activity ◽  
Subcellular Fractionation ◽  
Fatty Acyl ◽  
O2 Consumption ◽  
H2o2 Generation ◽  
Acyl Coa Oxidase ◽  
Acyl Coenzyme A ◽  
Rate Limiting ◽  
Peroxisomal Fatty Acid

It has been postulated that the peroxisomal fatty acid-oxidizing system [Lazarow & de Duve (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 2043–2046; Lazarow (1978) J. Biol. Chem. 253, 1522–1528] resembles that of mitochondria, except for the first oxidative reaction. In this step, O2 would be directly reduced to H2O2 by an oxidase. Two specific procedures developed to detect the activity of the characteristic enzyme fatty acyl-CoA oxidase are presented, namely polarographic detection of palmitoyl-CoA-dependent cyanide-insensitive O2 consumption and palmitoyl-CoA-dependent H2O2 generation coupled to the peroxidation of methanol in an antimycin A-insensitive reaction. Fatty acyl-CoA oxidase activity is stimulated by FAD, which supports the flavoprotein nature postulated for this enzyme. Its activity increases 7-fold per g wet wt. of liver in rats treated with nafenopin, a hypolipidaemic drug. Subcellular fractionation of livers from normal and nafenopin-treated animals provides evidence for its peroxisomal localization. The stoicheiometry for palmitoyl-CoA-dependent O2 consumption, H2O2 generation and NAD+ reduction is 1 : 1 : 1. This suggests that fatty acyl-CoA oxidase is the rate-limiting enzyme of the peroxisomal fatty acid-oxidizing system.

scholarly journals A sensitive spectrophotometric assay for peroxisomal acyl-CoA oxidase

1985 ◽  
Vol 227 (1) ◽  
pp. 205-210 ◽  
Author(s):  
G M Small ◽  
K Burdett ◽  
M J Connock
Keyword(s):  
Oxidase Activity ◽  
Mouse Liver ◽  
Enzyme Concentration ◽  
Fatty Acyl ◽  
Spectrophotometric Assay ◽  
Acid Oxidase ◽  
Rat Intestine ◽  
Amino Acid Oxidase ◽  
Acyl Coa Oxidase ◽  
First Time

A simple spectrophotometric assay was developed for peroxisomal fatty acyl-CoA oxidase activity. The assay, based on the H2O2-dependent oxidation of leuco-dichlorofluorescein catalysed by exogenous peroxidase, is more sensitive than methods previously described. By using mouse liver samples, cofactor requirements were assessed and a linear relationship was demonstrated between dye oxidation and enzyme concentration. By using this assay on subcellular fractions, palmitoyl-CoA oxidase activity was localized for the first time in microperoxisomes of rat intestine. The assay was also adapted to measure D-amino acid oxidase activity, demonstrating the versatility of this method for measuring activity of other H2O2-producing oxidases.

Responses in a Fathead Minnow (Pimephales promelas) Lifecycle Test and in Wild White Sucker (Catostomus commersoni) Exposed to a Canadian Bleached Kraft Mill Effluent

2010 ◽  
Vol 45 (2) ◽  
pp. 187-200 ◽  
Author(s):  
Joanne L. Parrott ◽  
L. Mark Hewitt ◽  
Tibor G. Kovacs ◽  
Deborah L. MacLatchy ◽  
Pierre H. Martel ◽  
...  
Keyword(s):  
Egg Production ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Fish Reproduction ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Liver Size ◽  
Paper Mill Effluent ◽  
Kraft Mill Effluent ◽  
Kraft Mill

Abstract To evaluate currently available bioassays for their use in investigating the causes of pulp and paper mill effluent effects on fish reproduction, the responses of wild white sucker (Catostomus commersoni) collected from the receiving environment at the bleached kraft mill at La Tuque, Quebec, were compared with responses of fathead minnow (Pimephales promelas) exposed to effluent in a laboratory lifecycle test. White sucker collected at effluent exposed sites had increased liver size but none of the reproductive effects that had been documented in earlier field studies at this site. Exposure to 1, 3, 10, 30, and 100% bleached kraft mill effluent (BKME) in the lab led to significantly decreased length, but increased weight and liver size in male fathead minnow. Female length was also decreased and liver size was increased at high effluent exposures. Most effluent concentrations (1 to 30%) significantly increased egg production compared with controls. The fathead minnow lifecycle assay mirrored the effects seen in wild fish captured downstream of the BKME discharge. These results will be used to select short-term fish tests for investigating the causes of and solutions to the effects of mill effluents on fish reproduction.

scholarly journals Identification and purification of a peroxisomal branched chain fatty acyl-CoA oxidase.

1991 ◽  
Vol 266 (36) ◽  
pp. 24676-24683
Author(s):  
P.P. Van Veldhoven ◽  
G. Vanhove ◽  
F. Vanhoutte ◽  
G. Dacremont ◽  
G. Parmentier ◽  
...  
Keyword(s):  
Fatty Acyl ◽  
Acyl Coa Oxidase ◽  
Branched Chain

scholarly journals nLethal and sublethal effects of diluted bitumen and conventional oil on fathead minnow (Pimephales promelas) larvae exposed during their early development

2021 ◽  
pp. 105884
Author(s):  
Roxanne Bérubé ◽  
Charles Gauthier ◽  
Thibault Bourdin ◽  
Marilou Bouffard ◽  
Gaëlle Triffault-Bouchet ◽  
...  
Keyword(s):  
Early Development ◽  
Fathead Minnow ◽  
Sublethal Effects ◽  
Pimephales Promelas ◽  
Conventional Oil
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