COMPARING THE SOLID PHASE AND SALINE EXTRACT MICROTOX® ASSAYS FOR TWO POLYCYCLIC AROMATIC HYDROCARBON–CONTAMINATED SOILS

2004 ◽  
Vol 23 (2) ◽  
pp. 245 ◽  
Author(s):  
Carolyn M. Acheson ◽  
Qin Zhou ◽  
Yonggui Shan ◽  
Gregory D. Sayles ◽  
Margaret J. Kupferle
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Contaminated Soils ◽  
Solid Phase ◽  
Saline Extract ◽  
Polycyclic Aromatic

Managing long-term polycyclic aromatic hydrocarbon contaminated soils: a risk-based approach

2013 ◽  
Vol 22 (12) ◽  
pp. 8927-8941 ◽  
Author(s):  
Luchun Duan ◽  
Ravi Naidu ◽  
Palanisami Thavamani ◽  
Jean Meaklim ◽  
Mallavarapu Megharaj
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Contaminated Soils ◽  
Polycyclic Aromatic

Comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soils

1997 ◽  
Vol 43 (4) ◽  
pp. 368-377 ◽  
Author(s):  
Fadi Dagher ◽  
Eric Déziel ◽  
Patricia Lirette ◽  
Gilles Paquette ◽  
Jean-Guy Bisaillon ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Contaminated Soils ◽  
Bacterial Strains ◽  
Chain Reaction ◽  
Pah Degradation ◽  
Catabolic Genes ◽  
Polycyclic Aromatic ◽  
Polymerase Chain

Five polycyclic aromatic hydrocarbon (PAH) degrading bacterial strains, Pseudomonas putida 34, Pseudomonas fluorescens 62, Pseudomonas aeruginosa 57, Sphingomonas sp. strain 107, and the unidentified strain PL1, were isolated from two contaminated soils and characterized for specific features regarding PAH degradation. Degradation efficiency was determined by the rapidity to form clearing zones around colonies when sprayed with different PAH solutions and the growth in liquid medium with different PAHs as sole source of carbon and energy. The presence of plasmids, the production of biosurfactants, the effect of salicylate on PAH degradation, the transformation of indole to indigo indicating the presence of an aromatic ring dioxygenase activity, and the hybridization with the SphAb probe representing a sequence highly homologous to the naphthalene dioxygenase ferredoxin gene nahAb were examined. The most efficient strain in terms of substrate specificity and rapidity to degrade different PAHs was Sphingomonas sp. strain 107, followed by strain PL1 and P. aeruginosa 57. The less efficient strains were P. putida 34 and P. fluorescens 62. Each strain transformed indole to indigo, except strain PL1. Biosurfactants were produced by P. aeruginosa 57 and P. putida 34, and a bioemulsifier was produced by Sphingomonas sp. strain 107. The presence of salicylate in solid medium has accelerated the formation of clearing zones and the transformation of indole by Sphingomonas sp. strain 107 and P. aeruginosa 57 colonies. Plasmids were found in Sphingomonas sp. strain 107 and strain PL1. The SphAb probe hybridized with DNA extracted from each strain. However, hybridization signals were detected only in the plasmidic fraction of Sphingomonas sp. strain 107 and strain PL1. Using a polymerase chain reaction (PCR) approach, we determined that several genes encoding enzymes involved in the upper catabolic pathway of naphthalene were present in each strain. Sequencing of PCR DNA fragments revealed that, for all the five strains, these genes are highly homologous with respective genes found in the pah, dox, and nah opérons, and are arranged in a polycistronic operon. Results suggest that these genes are ordered in the five selected strains like the pah, nah, and dox opérons.Key words: polycyclic aromatic hydrocarbons, biodegradation, polymerase chain reaction, naphthalene catabolic genes.

scholarly journals Comparison of plants with C3 and C4 carbon fixation pathways for remediation of polycyclic aromatic hydrocarbon contaminated soils

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Anithadevi Kenday Sivaram ◽  
Panneerselvan Logeshwaran ◽  
Suresh R. Subashchandrabose ◽  
Robin Lockington ◽  
Ravi Naidu ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Contaminated Soils ◽  
Carbon Fixation ◽  
Polycyclic Aromatic
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