scholarly journals Effect of Salt Stress on Growth and Ion Distribution of Rape Genotypes (Brassica napus L.) Differing in Salt Tolerance

2016 ◽  
Vol 6 (20) ◽  
pp. 71-83
Author(s):  
V. Atlassi Pak ◽  
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Salt Tolerance ◽  
Ion Distribution ◽  
Brassica Napus L

scholarly journals Gradual Exposure to Salinity Improves Tolerance to Salt Stress in Rapeseed (Brassica napus L.)

Water ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1667 ◽  
Author(s):  
Michael Santangeli ◽  
Concetta Capo ◽  
Simone Beninati ◽  
Fabrizio Pietrini ◽  
Cinzia Forni
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Salt Tolerance ◽  
Soil Salinity ◽  
Stress Responses ◽  
Prolonged Exposure ◽  
Relative Reduction ◽  
Brassica Napus L ◽  
Salt Stress Response ◽  
Psii Photochemistry

Soil salinity is considered one of the most severe abiotic stresses in plants; plant acclimation to salinity could be a tool to improve salt tolerance even in a sensitive genotype. In this work we investigated the physiological mechanisms underneath the response to gradual and prolonged exposure to sodium chloride in cultivars of Brassica napus L. Fifteen days old seedlings of the cultivars Dynastie (salt tolerant) and SY Saveo (salt sensitive) were progressively exposed to increasing soil salinity conditions for 60 days. Salt exposed plants of both cultivars showed reductions of biomass, size and number of leaves. However, after 60 days the relative reduction in biomass was lower in sensitive cultivar as compared to tolerant ones. An increase of chlorophylls content was detected in both cultivars; the values of the quantum efficiency of PSII photochemistry (ΦPSII) and those of the electron transport rate (ETR) indicated that the photochemical activity was only partially reduced by NaCl treatments in both cultivars. Ascorbate peroxidase (APX) activity was higher in treated samples with respect to the controls, indicating its activation following salt exposure, and confirming its involvement in salt stress response. A gradual exposure to salt could elicit different salt stress responses, thus preserving plant vitality and conferring a certain degree of tolerance, even though the genotype was salt sensitive at the seed germination stage. An improvement of salt tolerance in B. napus could be obtained by acclimation to saline conditions.

scholarly journals Evaluation of Salt Tolerance in Some Canola (Brassica napus L.( Genotypes under Normal and Salt Stress Conditions

2019 ◽  
Vol 11 (30) ◽  
pp. 23-36
Author(s):  
Irandokht Mansouri ◽  
Hamid Najafi Zarini ◽  
Nadali Babeian Jelodar ◽  
Ali Pakdin ◽  
◽  
...  
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Salt Tolerance ◽  
Stress Conditions ◽  
Brassica Napus L

scholarly journals A Study of Proline Metabolism in Canola (Brassica napus L.) Seedlings under Salt Stress

Molecules ◽  
2012 ◽  
Vol 17 (5) ◽  
pp. 5803-5815 ◽  
Author(s):  
Mubshara Saadia ◽  
Amer Jamil ◽  
Nudrat Aisha Akram ◽  
Muhammad Ashraf
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Proline Metabolism ◽  
Brassica Napus L

scholarly journals Molecular response of canola to salt stress: insights on tolerance mechanisms

2018 ◽  
Author(s):  
Reza Shokri-Gharelo ◽  
Pouya Motie-Noparvar
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Edible Oils ◽  
Biodiesel Fuel ◽  
Molecular Response ◽  
Salt Tolerant ◽  
Tolerance Mechanisms ◽  
Brassica Napus L ◽  
Salt Response ◽  
The Many

Canola (Brassica napus L.) is widely cultivated around the world for the production of edible oils and biodiesel fuel. Despite many canola varieties being described as ‘salt-tolerant’, plant yield and growth decline drastically with increasing salinity. Although many studies have resulted in better understanding of the many important salt-response mechanisms that control salt signaling in plants, detoxification of ions, and synthesis of protective metabolites, the engineering of salt-tolerant crops has only progressed slowly. Genetic engineering has been considered as an efficient method for improving the salt tolerance of canola but there are many unknown or little-known aspects regarding canola response to salinity stress at the cellular and molecular level. In order to develop highly salt-tolerant canola, it is essential to improve knowledge of the salt-tolerance mechanisms, especially the key components of the plant salt-response network. In this review, we focus on studies of the molecular response of canola to salinity to unravel the different pieces of the salt response puzzle. The paper includes a comprehensive review of the latest studies, particularly of proteomic and transcriptomic analysis, including the most recently identified canola tolerance components under salt stress, and suggests where researchers should focus future studies.

scholarly journals Chlorophyll a fluorescence as indicative of the salt stress on Brassica napus L.

2011 ◽  
Vol 23 (4) ◽  
pp. 245-253 ◽  
Author(s):  
Marcos Antonio Bacarin ◽  
Sidnei Deuner ◽  
Fabio Sergio Paulino da Silva ◽  
Daniela Cassol ◽  
Diolina Moura Silva
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Brassica Napus L

scholarly journals Overexpression of a Novel ROP Gene from the Banana (MaROP5g) Confers Increased Salt Stress Tolerance

2018 ◽  
Vol 19 (10) ◽  
pp. 3108 ◽  
Author(s):  
Hongxia Miao ◽  
Peiguang Sun ◽  
Juhua Liu ◽  
Jingyi Wang ◽  
Biyu Xu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Survival Rates ◽  
Wild Type ◽  
Ion Distribution ◽  
Membrane Injury ◽  
Primary Roots

Rho-like GTPases from plants (ROPs) are plant-specific molecular switches that are crucial for plant survival when subjected to abiotic stress. We identified and characterized 17 novel ROP proteins from Musa acuminata (MaROPs) using genomic techniques. The identified MaROPs fell into three of the four previously described ROP groups (Groups II–IV), with MaROPs in each group having similar genetic structures and conserved motifs. Our transcriptomic analysis showed that the two banana genotypes tested, Fen Jiao and BaXi Jiao, had similar responses to abiotic stress: Six genes (MaROP-3b, -5a, -5c, -5f, -5g, and -6) were highly expressed in response to cold, salt, and drought stress conditions in both genotypes. Of these, MaROP5g was most highly expressed in response to salt stress. Co-localization experiments showed that the MaROP5g protein was localized at the plasma membrane. When subjected to salt stress, transgenic Arabidopsis thaliana overexpressing MaROP5g had longer primary roots and increased survival rates compared to wild-type A. thaliana. The increased salt tolerance conferred by MaROP5g might be related to reduced membrane injury and the increased cytosolic K+/Na+ ratio and Ca2+ concentration in the transgenic plants as compared to wild-type. The increased expression of salt overly sensitive (SOS)-pathway genes and calcium-signaling pathway genes in MaROP5g-overexpressing A. thaliana reflected the enhanced tolerance to salt stress by the transgenic lines in comparison to wild-type. Collectively, our results suggested that abiotic stress tolerance in banana plants might be regulated by multiple MaROPs, and that MaROP5g might enhance salt tolerance by increasing root length, improving membrane injury and ion distribution.

scholarly journals RNAseq Analysis Reveals Altered Expression of Key Ion Transporters Causing Differential Uptake of Selective Ions in Canola (Brassica napus L.) Grown under NaCl Stress

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 891 ◽  
Author(s):  
Mobina Ulfat ◽  
Habib-ur-Rehman Athar ◽  
Zaheerud-din Khan ◽  
Hazem M. Kalaji
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Differentially Expressed Genes ◽  
Nacl Stress ◽  
Utilization Efficiency ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Salt Tolerant ◽  
Brassica Napus L ◽  
Salt Tolerant Cultivars

Salinity is one of the major abiotic stresses prevailing throughout the world that severely limits crop establishment and production. Every crop has an intra-specific genetic variation that enables it to cope with variable environmental conditions. Hence, this genetic variability is a good tool to exploit germplasms in salt-affected areas. Further, the selected cultivars can be effectively used by plant breeders and molecular biologists for the improvement of salinity tolerance. In the present study, it was planned to identify differential expression of genes associated with selective uptake of different ions under salt stress in selected salt-tolerant canola (Brassica napus L.) cultivar. For the purpose, an experiment was carried out to evaluate the growth response of different salt-sensitive and salt-tolerant canola cultivars. Plants were subjected to 200 mM NaCl stress. Canola cultivars—Faisal Canola, DGL, Dunkled, and CON-II—had higher growth than in cvs Cyclone, Ac-EXcel, Legend, and Oscar. Salt-tolerant cultivars were better able to maintain plant water status probably through osmotic adjustment as compared to salt-sensitive cultivars. Although salt stress increased shoot Na+ and shoot Cl− contents in all canola cultivars, salt-tolerant cultivars had a lower accumulation of these toxic nutrients. Similarly, salt stress reduced shoot K+ and Ca2+ contents in all canola cultivars, while salt-tolerant cultivars had a higher accumulation of K+ and Ca2+ in leaves, thereby having greater shoot K+/Na+ and Ca2+/Na+ ratios. Nutrient utilization efficiency decreased significantly in all canola cultivars due to the imposition of salt stress; however, it was greater in salt-tolerant cultivars—Faisal Canola, DGL, and Dunkled. Among four salt-tolerant canola cultivars, cv Dunkled was maximal in physiological attributes, and thus differentially expressed genes (DEGs) were assessed in it by RNA-seq analysis using next-generation sequencing (NGS) techniques. The differentially expressed genes (DEG) in cv Dunkled under salt stress were found to be involved in the regulation of ionic concentration, photosynthesis, antioxidants, and hormonal metabolism. However, the most prominent upregulated DEGs included Na/K transporter, HKT1, potassium transporter, potassium channel, chloride channel, cation exchanger, Ca channel. The RNA-seq data were validated through qRT-PCR. It was thus concluded that genes related to the regulation of ionic concentrate are significantly upregulated and expressed under salt stress, in the cultivar Dunkled.

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