Comparison of Biochemical Responses of Common Vetch (Vicia sativa L.) Seedling Organs to Salinity

2021 ◽  
Author(s):  
Ramazan Beyaz
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Proline Accumulation ◽  
Root Tissue ◽  
Antioxidant Enzyme Activities ◽  
Common Vetch ◽  
Essential Information ◽  
Mda Content ◽  
Antioxidant Enzymes Activities ◽  
Root Tissues

Background: Shoots and roots are autotrophic and heterotrophic organs of plants with different physiological and biochemical functions under stress conditions. The metabolites involved in tolerance enhancement differed between roots and shoots. In this study, the biochemical changes occurring in shoot and root organs under salt stress and the level of these changes were investigated. However, these changes in shoot and root organs were compared.Methods: Seeds of common vetch were sown and subjected to 14 days of salt stress in basal MS medium containing 100 mM NaCl. In shoot and root tissue, biochemical parameters such as antioxidant enzymes activities (GR, APX, SOD and CAT), malondialdehyde (MDA) content and proline accumulation were determined.Result: Results of the study indicated that the activities of antioxidant enzymes (SOD, CAT (except in shoot), GR and APX), MDA and proline accumulation enhanced by salt stress in both organs. On the other hand, morphological parameters decreased in both tissues. It seemed that antioxidant enzyme activities more active in root tissues. However, proline accumulation was found higher in shoot tissues than root tissue, while MDA content was higher in root tissue than shoot tissue. The present investigation provides essential information for the antioxidant components of the shoot and root organs of vetch seedlings under salt stress.

scholarly journals Impacts of drought stress on growth, protein, proline, pigment content and antioxidant enzyme activities in rice (Oryza sativa L. var. BRRI dhan-24)

2020 ◽  
Vol 29 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Shamima Nasrin ◽  
Shukanta Saha ◽  
Hasna Hena Begum ◽  
Rifat Samad
Keyword(s):  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Oryza Sativa L ◽  
Proline Accumulation ◽  
The Other ◽  
Antioxidant Enzyme Activities ◽  
Total Chlorophyll ◽  
Enzymes Activities ◽  
Pigment Contents ◽  
Antioxidant Enzymes Activities

One of the major problems associated with rice cultivation and production is the deficiency of water resources. In here the effect of drought stress on growth, protein-, proline-, pigment contents and antioxidant enzymes activities in rice var. BRRI Dhan-24 was determined and analyzed under drought and wellwatered condition. Drought stress caused the decrease of growth and pigment contents - chlorophyll-a, b, a/b, total chlorophyll and carotenoids content of leaves of rice plants. On the other hand, the accumulation of protein, proline contents and antioxidant enzymes activities was increased under drought stress. It may be suggested that antioxidant enzymes activities and proline accumulation were associated with the growth of the plant and consequently with the mechanisms of drought tolerance in rice. Dhaka Univ. J. Biol. Sci. 29(1): 117-123, 2020 (January)

scholarly journals Biochemical Responses of Shoot and Root Tissues of Sainfoin (Onobrychis viciifolia Scop.) to NaCl-Salt Stress Under İn Vitro Conditions

2019 ◽  
Vol 7 (1) ◽  
pp. 110
Author(s):  
Ramazan Beyaz
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Root Tissue ◽  
Ms Medium ◽  
Forage Crops ◽  
Sod Activity ◽  
Onobrychis Viciifolia ◽  
Biochemical Responses ◽  
Root Tissues

Sainfoin (Fabaceae) is one of the most critical animal forage crops. However, the tolerance of sainfoin is low against to salinity. This study aims to investigate biochemical responses of the shoot and root tissue of sainfoin seedlings to moderate salt stress under in vitro conditions. For this aim, the seed of sainfoin were sown MS medium containing 100 mM NaCl. Antioxidant enzymes (CAT, SOD, APX, and GR), proline and malondialdehyde (MDA) contents were measured in shoot and root tissue of 35-day-old seedlings of sainfoin. A significantly higher constitutive catalase (CAT) and superoxide dismutase (SOD) activity was observed in shoot tissues when compared to root tissues. Overall, salt stress caused significant more enhancement in the activity of antioxidant enzymes (CAT, SOD, APX, and GR) in shoot tissues than root tissue. On the other hand, among the antioxidant enzymes, SOD seems to be more active in both tissues of sainfoin. Interestingly, the activity of GR reduced in both tissue under salt stress. The content of proline and MDA has been increased under salt stress and this increase has been more in the root tissue. This study has revealed biochemical responses to salt stress in different organs of sainfoin.

Biochemical responses of sainfoin shoot and root tissues to drought stress in In vitro Culture

2019 ◽  
Author(s):  
Ramazan Beyaz
Keyword(s):  
Drought Stress ◽  
Critical Role ◽  
Root Tissue ◽  
Antioxidant Enzyme Activities ◽  
Chlorophyll Contents ◽  
Biochemical Responses ◽  
Shoot Tissue ◽  
Mda Content ◽  
Root Tissues

This study was conducted to investigate the biochemical responses of the shoot and root tissues of sainfoin to drought stress under in vitro conditions. Seeds of sainfoin were cultured on MS (Murashige and Skoog) medium with addition of concentrations of PEG-6000 (50, 100, and 150 g/l). Biochemical analyzes (CAT, SOD, GR, and APX enzyme activity; proline, malondialdehyde (MDA) and chlorophyll contents) were carried out on the 35-day-old seedlings. The principal results of the study were that CAT and SOD antioxidant enzymes seemed to play a critical role in oxidative stress in both tissues of sainfoin seedlings. On the other hand, a significant decrease in GR activity and no change in APX activity detected in both tissues under stress. The contents of proline and MDA increased in both tissues while the chlorophyll contents decreased in the shoot tissue. Antioxidant enzyme activities seemed to be more active in the root tissue than the shoot tissue. Accumulation of proline was higher in the root tissue, while the MDA content was higher in the shoot tissue of the seedlings.

scholarly journals Melatonin Application Improves Salt Tolerance of Alfalfa (Medicago sativa L.) by Enhancing Antioxidant Capacity

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 220 ◽  
Author(s):  
Huifang Cen ◽  
Tingting Wang ◽  
Huayue Liu ◽  
Danyang Tian ◽  
Yunwei Zhang
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Medicago Sativa ◽  
Salt Resistance ◽  
Primary Role ◽  
Waterlogging Tolerance ◽  
Alfalfa Seed ◽  
Mda Content ◽  
Medicago Sativa L

Alfalfa (Medicago sativa L.) is an important and widely cultivated forage grass. The productivity and forage quality of alfalfa are severely affected by salt stress. Melatonin is a bioactive molecule with versatile physiological functions and plays important roles in response to various biotic and abiotic stresses. Melatonin has been proven efficient in improving alfalfa drought and waterlogging tolerance in recent studies. In our reports, we applied melatonin exogenously to explore the effects of melatonin on alfalfa growth and salt resistance. The results demonstrated that melatonin application promoted alfalfa seed germination and seedling growth, and reduced oxidative damage under salt stress. Further application research found that melatonin alleviated salt injury in alfalfa plants under salt stress. The electrolyte leakage, malondialdehyde (MDA) content and H2O2 content were significantly reduced, and the activities of catalase (CAT), peroxidase (POD), and Cu/Zn superoxide dismutase (Cu/Zn-SOD) were increased with melatonin pretreatment compared to control plants under salt stress with the upregulation of genes related to melatonin and antioxidant enzymes biosynthesis. Melatonin was also involved in reducing Na+ accumulation in alfalfa plants. Our study indicates that melatonin plays a primary role as an antioxidant in scavenging H2O2 and enhancing activities of antioxidant enzymes to improve the salt tolerance of alfalfa plants.

scholarly journals Effects of drought stress on pigment and protein contents and antioxidant enzyme activities in five varieties of rice (Oryza sativa L.)

2020 ◽  
Vol 49 (4) ◽  
pp. 997-1002
Author(s):  
Shukanta Saha ◽  
Hasna Hena Begum ◽  
Shamima Nasrin ◽  
Rifat Samad
Keyword(s):  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Protein Content ◽  
Chlorophyll A ◽  
Pigment Content ◽  
Antioxidant Enzyme Activities ◽  
Rice Varieties ◽  
Enzymes Activities ◽  
Drought Tolerant ◽  
Antioxidant Enzymes Activities

Drought stress caused the decrease of pigment contents - chlorophyll a, chlorophyll b, chlorophyll a/b ratio, total chlorophyll content and carotenoids content of leaves of five rice varieties (var. BRRI Dhan-30, BRRI Dhan-32, BRRI Dhan-34, BRRI Dhan-38 and BRRI Dhan-56). Among five rice varieties, BRRI Dhan- 56 showed the least decrease of pigment content under stress. It caused the decrease of protein content of leaves of five rice varieties whereas BRRI Dhan-56 showed the least decrease (3.64%) of protein content under stress. On the other hand, drought stress increased CAT and SOD activities in the leaf of five varieties of rice and BRRI Dhan-56 showed the highest increase of CAT (37.67%) and SOD (94.17%) activity under stress. It may be assumed that higher antioxidant enzymes activities (CAT and SOD) and less reduction of protein content were related to the mechanisms of drought tolerance in rice. It is indicated that BRRI Dhan- 56 may be drought tolerant while BRRI Dhan-30, BRRI Dhan-32, BRRI Dhan-34 and BRRI Dhan-38 were drought sensitive rice varieties. Drought tolerant rice variety is selected based on pigment content, protein content and antioxidant enzymes activities (CAT and SOD).

scholarly journals Effects of ABA on Physiological Characteristics of Tomato under Waterlogging

2020 ◽  
Vol 189 ◽  
pp. 02007
Author(s):  
Wei Gao ◽  
Dongmei Yin ◽  
Zhuqing Han
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Antioxidant Enzyme Activities ◽  
Deficient Mutant ◽  
Waterlogging Tolerance ◽  
New Yorker ◽  
Waterlogging Stress ◽  
Mda Content ◽  
Waterlogging Treatment ◽  
Pot Plants

The effects of waterlogging on antioxidant enzyme activities responses in five different lines, i.e. ABA-deficient mutant (0673) and its control (0535), two ABA over-production transgenic rd29A:NCED1 lines (#2, #7) and Mill. L. cv. New Yorker (WT) were investigated. The waterlogging was mimicked by treating pot plants with flooding. The malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity in the leaves were evaluated in all treatments. The results showed that after 7 days waterlogging treatment, the malondialdehyde (MDA) was increased in all plants, especially in LA0673. Compared with the LA0673, #2 and #7 significantly increase the activities of SOD, POD and CAT under waterlogging stress. Therefore, ABA could improve the waterlogging tolerance of tomato by increasing the activities of antioxidant enzymes under waterlogging stress.

scholarly journals Alleviative effects of Fara-darmani Consciousness Field on Triticum aestivum L. under salinity stress

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1089 ◽  
Author(s):  
Sara Torabi ◽  
Mohammad Ali Taheri ◽  
Farid Semsarha
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Scientific Evidence ◽  
Intervention Strategy ◽  
Triticum Aestivum L ◽  
Antioxidant Enzyme Activities ◽  
Total Chlorophyll ◽  
Controlled Experiments ◽  
Mda Content ◽  
Chlorophyll Forms

Background: The Fara-darmani Consciousness Field was founded by Mohammad Ali Taheri. It is a novel field and is described similarly to the field of gravity, or the electromagnetic field. This field is neither matter nor energy, and therefore does not possess a quantity. Even though there is no direct scientific evidence for the Consciousness Field, it is possible to investigate its effects on objects through controlled experiments. The aim of the present work was to study the alleviative effects of the Fara-darmani Consciousness Field on common wheat Triticum aestivum L. var Star under salt stress. Methods: Plants were grown under 0 mM NaCl (control) and 150 mM NaCl with or without the influence of Fara-darmani Consciousness Field for 3 weeks. Chlorophyll, hydrogen peroxide (H2O2), malondialdehyde (MDA) content and activity of antioxidant enzymes such as superoxide dismutase (SOD),polyphenol oxidase (PPO), and peroxidase (POX) were measured in all groups of plants. Results: In the salt-treated plants under the influence of the Fara-darmani Consciousness Field, the contents of total chlorophyll, as well as a and b chlorophyll forms, were elevated compared with the salt-treated plants without Fara-darmani CF (34.8%, 17.8%, and 169% respectively). Additionally, Fara-darmani increased H2O2 (57%) and the activity of SOD and PPO by 220% and 168%, respectively, under salinity compared with the salt-treated plants without Fara-darmani CF. MDA content and activity of peroxidase were decreased by 12.5% and 34%, respectively. Conclusion: These results suggest the Fara-darmani Consciousness Field as a qualitative intervention strategy to withstand salt stress in plants, by increasing the contents of chlorophyll, antioxidant enzyme activities, and decreasing MDA content under salinity.

Biological variability of superoxide dismutase, glutathione peroxidase, and catalase in blood

1991 ◽  
Vol 37 (11) ◽  
pp. 1932-1937 ◽  
Author(s):  
L Guemouri ◽  
Y Artur ◽  
B Herbeth ◽  
C Jeandel ◽  
G Cuny ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
The Elderly ◽  
Reference Intervals ◽  
Antioxidant Enzyme Activities ◽  
Biological Variability ◽  
Sod Activity ◽  
Antioxidant Enzymes Activities ◽  
Automated Methods

Abstract We studied the biological variability of blood superoxide dismutase (SOD; EC 1.15.1.1), glutathione peroxidase (GPX; EC 1.11.1.9), and catalase (CAT; EC 1.11.1.6) in a sample of 1836 apparently health subjects, ages 4-97 years. SOD and GPX activities were assayed in plasma (P) and erythrocytes (E) by automated methods, and CAT was measured in erythrocytes by a manual technique. No statistically significant variation of these antioxidant enzyme activities according to gender was demonstrated, except for E-GPX, which was slightly but significantly higher in women than in men (P less than 0.001). Activities appear rather stable in adults less than 65 years old, but decrease for most enzymes in the elderly. There is no evidence that weight, blood pressure, or menopause influences the antioxidant enzymes' activities. In girls ages 10-14 years, E-SOD activity is reduced by 16% (P less than 0.05) after menarche. Variations related to smoking and alcohol consumption are slight and concern only P-SOD and P-GPX, respectively. Conversely, intake of some drugs (e.g., anti-inflammatory agents, antidepressants, and thyroid hormones) modifies activity of some of the three enzymes. E-SOD positively correlates with P-SOD (r = 0.216, P less than 0.001) and E-CAT (r = 0.123, P less than 0.001), and E-GPX with P-GPX (r = 0.218, P less than 0.001). Finally, we propose reference intervals for activities of the three antioxidant enzymes in blood in individuals less than 65 years old.

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