Influence of Boron Priming on the Antioxidant Ability of Alfalfa Seeds

2020 ◽  
Author(s):  
F. S. Xia ◽  
F. Wang ◽  
Y. C. Wang ◽  
C. C. Wang ◽  
R. Tian ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Antioxidant Ability ◽  
Sensitive Site ◽  
Borax Solution ◽  
The Relationship ◽  
Seeds Germination ◽  
Alfalfa Seeds

This experiment was designed to determine the relationship between the antioxidant capacity and the vigour of alfalfa seeds during boron priming. Alfalfa seeds were primed with 1.8 % (W/V) concentration of borax solution for 0, 3, 6, 12 and 24 h at 20°C. The results showed that the vigour of alfalfa seeds declined with the extension of priming time, which was closely related to the accumulation of hydrogen peroxide and malonaldehyde contents and the decrease of superoxide dismutase, catalase and peroxidase. The radicle might be the most sensitive site to boron priming in alfalfa seeds, but the boron-induced damage might be alleviated during alfalfa seeds’ germination.

Inhibition of Phagocytosis-Associated Chemiluminescence by Superoxide Dismutase

1974 ◽  
Vol 9 (6) ◽  
pp. 1051-1056
Author(s):  
Lawrence S. Webb ◽  
Bernard B. Keele ◽  
Richard B. Johnston
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Hydroxyl Radical ◽  
Bactericidal Activity ◽  
Liquid Scintillation ◽  
Superoxide Anions ◽  
Liquid Scintillation Spectrometer ◽  
Human Leukocytes ◽  
Enzyme Superoxide Dismutase ◽  
The Relationship

During the process of phagocytosis, human leukocytes emit a burst of luminescence which can be measured in a liquid scintillation spectrometer. The enzyme superoxide dismutase, which removes superoxide anions (O · ), inhibited this chemiluminescence by 70% at a concentration of 100 μg/ml. The enzyme did not inhibit phagocytosis. These results support other studies indicating that O · is elaborated by phagocytizing leukocytes. They also indicate that O · plays a major role in phagocytosis-associated chemiluminescence, though not necessarily as the luminescing agent. Catalase and benzoate inhibited the chemiluminescence of phagocytosis to a slight extent, suggesting that hydrogen peroxide and hydroxyl radical, respectively, might also be involved in this phenomenon. The relationship between the mediators of chemiluminescence and those responsible for phagocytic bactericidal activity remains to be defined.

Effect of Val 73 → Trp Mutation on the Reaction of “Cambialistic” Superoxide Dismutase fromPropionibacterium shermaniiwith Hydrogen Peroxide

1997 ◽  
Vol 345 (1) ◽  
pp. 156-159 ◽  
Author(s):  
R. Gabbianelli ◽  
A. Battistoni ◽  
C. Capo ◽  
F. Polticelli ◽  
G. Rotilio ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase

scholarly journals Antioxidant Capacity and Superoxide Dismutase Activity in Adrenoleukodystrophy

2017 ◽  
Vol 74 (5) ◽  
pp. 519 ◽  
Author(s):  
Bela R. Turk ◽  
Benjamin E. Theisen ◽  
Christina L. Nemeth ◽  
Joel S. Marx ◽  
Xiaohai Shi ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Superoxide Dismutase Activity

scholarly journals Cromoglycate and nedocromil enhanced the reactive oxygen species-dependent suppressions with, but not without, dexamethasone in ischaemic and histamine paw oedema of mice

1997 ◽  
Vol 6 (5-6) ◽  
pp. 369-374
Author(s):  
Y. Oyanagui
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Glucocorticoid Receptor ◽  
Low Dose ◽  
Natural Antioxidant ◽  
Target Cells ◽  
Oxygen Species ◽  
Paw Oedema ◽  
Β Carotene

Anti-inflammatory actions of two anti-allergic drugs, alone or with dexamethasone (Dex) were examined in two models, because inflammation is claimed to be important for allergic events, especially for asthma. Cromoglycate and nedocromil were tested in ischaemic- and histamineinduced paw oedema models of mice. These antiallergic drugs (1–100 mg/kg, i.p.) failed to suppress these oedemata, but enhanced the suppressions by a low dose of dexamethasone (0.1 mg/kg, s.c.) at 3–8 h after Dex injection. The mode of effects by anti-allergic drugs resembled that of a natural antioxidant (α-tocopherol, β-carotene etc.), and was different from that of an immunosuppressant like FK506. The enhancing potencies of the two anti-allergic drugs were similar at 6 h after Dex in both oedemata, and were diminished by superoxide dismutase (SOD) or catalase (i.p.). Cycloheximide completely abolished suppressions. Nedocromil, but not cromoglycate, inhibits inflammatory events. Therefore, there are common unknown actions by which the two anti-allergics enhance suppression by Dex. A possible mechanism of this action was supposed to enhance the superoxide and/or hydrogen peroxide-dependent glucocorticoid receptor (GR) signalling in the target cells.

Catalase released from beneficial and plant-pathogenic pseudomonads by water and chloroform treatments

1994 ◽  
Vol 40 (8) ◽  
pp. 630-636
Author(s):  
J. I. Pounder ◽  
A. J. Anderson
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Water Treatment ◽  
Pseudomonas Syringae ◽  
Small Proportion ◽  
Plant Colonization ◽  
Periplasmic Proteins ◽  
Specific Activities ◽  
Leaf Pathogen ◽  
Glucose 6 Phosphate Dehydrogenase

Survival of pseudomonads during plant colonization may involve bacterial catalases to degrade the hydrogen peroxide produced by the plant. The specific activities of catalases in lysates from two saprophytic isolates of Pseudomonas putida and Pseudomonas fluorescens and three races of Pseudomonas syringae pv. glycinea were similar. To explore the location of the bacterial catalases, cells of the pathogenic and saprophytic pseudomonads were treated with chloroform, which is reported to release periplasmic proteins. Although catalase was released by chloroform treatment, the cytoplasmic enzymes isocitrate dehydrogenase, superoxide dismutase, and glucose-6-phosphate dehydrogenase were also detected. These proteins may have come from lysis of a small proportion of the cells rather than the periplasm. Water treatment of cells also released amounts of protein similar to those derived from chloroform treatment. Similar responses were found from both pathogenic and saprophytic strains. The release of catalase and proteins from the leaf pathogen P. syringae pv. glycinea race 0 and the root-associated saprophyte P. putida decreased as the cultures aged. With P. putida and P. syringae pv. glycinea race 0, the single isozyme of catalase released by water and chloroform treatment also was detected in lysates. Additional catalase isozymes were present in lysates as the cultures aged.Key words: periplasmic proteins, survival.

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