Effects of Super Absorbent Polymers and Gibberellin on Germination and Growth of alfalfa

2020 ◽  
Author(s):  
Wenxu Zhang ◽  
Jing Chen ◽  
Jinghe Tao ◽  
Peng Wang ◽  
Qian Liu ◽  
...  
Keyword(s):  
Survival Rate ◽  
Water Supply ◽  
Germination Rate ◽  
Dry Weight ◽  
Shoot Length ◽  
Fresh Weight ◽  
Promoting Effect ◽  
Super Absorbent Polymer ◽  
Absorbent Polymer ◽  
Germination And Growth

For proven effects of eco-friendly super absorbent polymer and gibberellin on germination and growth of alfalfa, taking the germination and growth test in the artificial climate box by adding the different amount of super absorbent polymer and gibberellin. Observed the germination potential, germination rate, shoot length, root length, fresh weight and dry weight, under the different experiment conditions. The test showed that the super absorbent polymer had a promoting effect on alfalfa. The property of slowly release water, water supply on super absorbent polymer could keep plants prolonging the survival rate in the case of insufficient water supply. It could improve the germination rate of the seed of alfalfa. Adding super absorbent polymer and gibberellin not only improved the survival rate, but also promoted the growth of root and shoot on alfalfa.

scholarly journals Selenyum Uygulaması ile Yetiştirilen Adaçayı Bitkisinden Elde Edilen Hidrosollerin Çemen ve Kişniş Tohumlarının Çimlenmesi Üzerine Etkisi

2019 ◽  
Vol 7 (sp2) ◽  
pp. 36
Author(s):  
Ferit Özen ◽  
Gülsüm Yaldız ◽  
Mahmut Çamlıca ◽  
Halit Aşkın ◽  
Abdurrahman Başol
Keyword(s):  
Root Length ◽  
Germination Rate ◽  
Dry Weight ◽  
Shoot Length ◽  
Fresh Weight ◽  
Fenugreek Seeds ◽  
Mean Values ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
The Mean

This research was carried out in the climate chamber in order to determine the effect of hydrosols obtained from sage grown with selenium application on germination of fenugreek and coriander seeds. Different concentrations of sage hydrosol (2.5, 5, 7.5 and 10 ml) and purified water used as control were applied to the fenugreek and coriander seeds. Germination rate, fresh weight, dry weight, root length, shoot length and root / shoot ratio of fenugreek and coriander seeds were applied. Variance analysis was performed according to the split plot design and the mean values were compared with LSD test. According to the results of the research; germination rate of fenugreek seeds 38.35-51.00%, fresh weight 0.04-0.12 g, dry weight 0.001-0.035 g, root length 0.71-2.02 cm, shoot length 1.27-3.20 cm, root/shoot ratio varied between 0.34-0.82, germination rate of coriander seeds 18.61-39.21%, fresh weight 0.0011-0.034 g, dry weight 0.004-0.030 g, root length 1.5-4.58 cm, shoot length 1.08-3.69 cm, root/shoot ratio 0.47-2.51 were found. The highest germination rate was obtained in 2.5 ml dose of sage hydrosol (51.00%) in fenugreek seeds and the highest germination rate was found in 7.5 ml dose of sage hydrosol (39.21%) in coriander seeds.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

scholarly journals The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Principal Component ◽  
Dry Weight ◽  
Seed Priming ◽  
Nacl Stress ◽  
Future Research ◽  
Fresh Weight ◽  
Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

scholarly journals Effect of N-methyl-N-nitro-N-nitrosoguanidine (NTG) on callus induction and survival rate on mature embryos of beans (Phaseolus vulgaris)

2009 ◽  
Vol 3 (2) ◽  
pp. 91-98
Author(s):  
Sattar A. Shlahi ◽  
Zahra N. Hashim Al- Hattab
Keyword(s):  
Survival Rate ◽  
Callus Induction ◽  
Indole Acetic Acid ◽  
Casein Hydrolysate ◽  
Dry Weight ◽  
Ms Medium ◽  
Fresh Weight ◽  
Benzyl Adenine ◽  
Mature Embryos ◽  
Survival Percentage

This research was conducted to study the effect of the chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine on the percentage of callus induction and survival from mature beans embryos harvester cultivar. Seeds were treated with (0.2 or 0.4) millimolar of the mutagen NTG in combination with 0.0, 4 or 8% of ethanol, pH 5 ±2 0. for 24 h. Calli were induced on mature embryos by using MS medium with 0.5 mg/l of Benzyl adenine (BA), 1 mg/l Indole acetic acid (IAA) and 100 mg/l from each of Casein hydrolysate, Glycine, Asparagine, Tyrosine, and Myo-Inositol. Results showed that the hypocotyl surpassed the radical and the plume significantly in terms of survival reached 56.3%. Mutagen treatments showed asignificant effect on calli survival. Treatment with 8% Ethanol was lethal for all explants. While treatment with 0.4 mM NTG without Ethanol gaved the highest survival rate. The interaction between the treatments and the explants showed that the lowest survival percentage was which 8.8% that was for shoots treated with 0.2 mM of 4% Ethanol. Calli induced on hypocotyls treated with 0.4 mM NTG without Ethanol gave the highest fresh weight (347.2) mg while the lowest was (60) mg for calli induced on the radical treated with 0.4 mM NTG with 4% Ethanol. Moreover the highest dry weight was 22.5 mg for calli induced from hypocotyls treated with 0.4 millimolar NTG without Ethanol that was higher than the control 17.2 mg.The lowest dry weight obtained from calli induced on the radical treated with 0.4 mM NTG with 4% Ethanol was 3 mg. In conclusion the results showed that 0.4 mM NTG without Ethanol gave the highest survival rate and the highest fresh and dry weight for calli induced on the hypocotyl.

The effect of two audible sound frequencies on the germination and growth of a spring and winter wheat

1968 ◽  
Vol 46 (9) ◽  
pp. 1151-1158 ◽  
Author(s):  
Pearl Weinberger ◽  
Mary Measures
Keyword(s):  
Growth Response ◽  
Germination Rate ◽  
Dry Weight ◽  
Continuous Exposure ◽  
Subsequent Growth ◽  
Sound Exposure ◽  
Audible Sound ◽  
Total Plant ◽  
Accelerated Growth ◽  
Germination And Growth

The effect of sound of 5 kc/s and 12 kc/s on the germination and growth of grains and seedlings of vernalized spring (Marquis) and winter (Rideau) wheats was investigated. Untreated controls were simultaneously followed, and all experimental series were replicated at least three times. Growth cabinets were systematically interchanged with the replications to eliminate other variables. Sound at both frequencies significantly stimulated the germination of Marquis grains maintained at 2° and 10 °C. At 25 °C the rate of germination was unchanged. An indication of a slightly elevated germination rate was observed in Rideau grains held at 2° and exposed to 5 kc/s. Neither frequency significantly affected germination at 10° or 25 °C.Growth of plants derived from fully vernalized grains was followed by measuring total plant height, wet and dry weight of "tops", number of roots, and their fresh and dry weights. Rideau wheat grew more vigorously at both sound frequencies. Continuous exposure to 5 kc/s both during vernalization and subsequent growth resulted in a 200% or greater increase in all parameters followed. The growth response of Marquis wheat was dependent upon treatment. Some conditions of sound exposure accelerated growth, whereas in others growth was depressed.

scholarly journals In vitro screening of rice genotypes using polyethylene glycol under drought stress

2016 ◽  
Vol 27 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J Akte ◽  
S Yasmin ◽  
MJH Bhuiyan ◽  
F Khatun ◽  
J Roy ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Shoot Length ◽  
Fresh Weight ◽  
Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

scholarly journals Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hassan O. Shaikhaldein ◽  
Fahad Al-Qurainy ◽  
Mohammad Nadeem ◽  
Salim Khan ◽  
Mohamed Tarroum ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Chlorophyll Content ◽  
Total Protein ◽  
Dry Weight ◽  
Shoot Length ◽  
Antioxidant Enzyme Activities ◽  
Fresh Weight ◽  
Wide Range ◽  
Shoot Number

Abstract Silver nanoparticles (AgNPs) are presently the most commonly generated engineered nanomaterials and are found in a wide range of agro-commercial products. The present study was designed to synthesize AgNPs biologically using Ochradenus arabicus leaves and investigate their effect on the morphophysiological properties of Maerua oblongifolia raised in vitro. Physicochemical methods (ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy were performed for characterization and for obtaining microphotographs of the AgNPs. Shoots of M. oblongifolia (2–3 cm) grown in Murashige and Skoog medium supplemented with different concentrations of AgNPs (0, 10, 20, 30, 40, or 50 mg L−1) were used. Following 6 weeks of in vitro shoot regeneration, the shoot number, shoot length, leaf number, fresh weight, dry weight, chlorophyll content, total protein, proline level, and antioxidant enzyme activities of the plants were quantified. We found that 20 mg L−1 AgNPs increased the shoot number, shoot length, fresh weight, dry weight, and chlorophyll content of the plants. The maximum total protein was recorded in plants that were administered the lowest dose of AgNPs (10 mg L−1), while high concentrations of AgNPs (40 and 50 mg L−1) increased the levels of proline and the enzymes superoxide dismutase and catalase. Our results indicate that green-synthesized AgNPs may be of agricultural and medicinal interest owing to their effects on plants in vitro.

scholarly journals Effect of Water Extract Originated from Different Part of Russian Knapweed (Acroptilon repens L.) on Growth of Purslane (Portulaca oleracea L.)

2012 ◽  
Vol 4 (2) ◽  
pp. 108-111
Author(s):  
Alireza PIRZAD ◽  
Mousa JAMALI ◽  
Mohammad Amin ZAREH ◽  
Fahime SHOKRANI
Keyword(s):  
Aerial Part ◽  
Water Extract ◽  
Dry Weight ◽  
Shoot Length ◽  
Portulaca Oleracea ◽  
Control Treatment ◽  
Fresh Weight ◽  
Extract Concentration ◽  
Russian Knapweed ◽  
Seedling Length

To evaluate probable allelopathic effect of water extract originated from different parts of russian knapweed (Acroptilon repens L.) on growth of purslane (Portulaca oleracea L.), a factorial experiment was conducted based on randomized complete block design with three replications at greenhouse condition in 2011. Treatments were water extract concentrations (0, 5, 10, 15 and 20 percent) obtained from different part of russian knapweed (aerial part, flower and root). Results showed the significant effect of extract type on seedling length, and extract concentration on shoot length, root length and seedling length. Interaction effect between extract type and concentration on the ratio of root/shoot length, seedling fresh weight, seedling dry weight and germination percentage was significant, too. Means comparison indicated that the longest root (3.55 cm), shoot (4.65 cm) and seedling (8.20 cm) were obtained from control treatment, reducing with higher concentration of extract. The longest shoot (6.95 cm) belonged to extract originated from russian knapweed flowers. The highest ratio of root/shoot length (0.73), seedling fresh weight (0.61g) and seedling dry weight (0.044 g) belonged to control treatment. In general, increasing of extract concentration caused in reduction of germination and seedling traits. Meanwhile, extracts of aerial part had more sever reducing effect than root and flower extracts.

scholarly journals Selection Criteria for Drought-Tolerant Bread Wheat Genotypes at Seedling Stage

2019 ◽  
Vol 11 (9) ◽  
pp. 2584 ◽  
Author(s):  
Hafiz Ahmed ◽  
Muhammad Sajjad ◽  
Mingju Li ◽  
Muhammad Azmat ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Selection Criteria ◽  
Dry Weight ◽  
Shoot Length ◽  
Seedling Stage ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Drought Tolerant ◽  
Selection For ◽  
Thermo Stability

Diminishing water resources as a result of excessive use of water for irrigation and climate change posture a severe global threat to food security. Herein, an experiment was conducted to determine the selection criteria for drought-tolerant bread wheat genotypes at the seedling stage using morphological and photosynthetic pigmentation-related traits. A panel of 105 wheat landraces, historical Pakistani varieties, and advance breeding lines were evaluated under normal and drought stress using factorial completely randomized design. The root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b were positively correlated among themselves under both normal and stress conditions. Hence, selection of any one of these traits enhances the performance of other traits. The shoot length was non-significant and negatively associated with all other studied characters except relative water content. The results suggested that selection for shoot length could not improve genetic gain for drought tolerance. Out of 10 principal components (PCs), the first three PCs were showed significant genetic variation under both conditions. The first three PCs showed 74.6% and 76% cumulative genetic variation under normal and drought conditions, respectively. Based on PCA, 10 drought-tolerant and five drought-susceptible genotypes were identified. Overall results suggested that selection for root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b at the seedling stage would improve genetic gain for drought tolerance. The outperforming genotypes under drought stress conditions can be useful in future wheat breeding programs, and early selection for the traits recommended in this study will be effective for developing high-yielding and drought-tolerant wheat varieties.

scholarly journals Salinity Tolerance of Mungbean Genotypes at Seedling Stage

2018 ◽  
Vol 10 (2) ◽  
pp. 409-416
Author(s):  
Ratri Tri Hapsari ◽  
Trustinah Trustinah
Keyword(s):  
Germination Rate ◽  
Lateral Roots ◽  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seedling Stage ◽  
Fresh Weight ◽  
Vigor Index ◽  
Normal Seedling ◽  
Two Factors

Salinity is a major abiotic stress limiting mungbean production worldwide including Indonesia. Since mungbean plant is very sensitive to salt condition, selection of salinity tolerant genotypes becomes important for mungbean improvement. The objective of this study was to evaluate the tolerance of eight mungbean genotypes to salinity at seedling stage under different levels. The experiment was arranged in a randomized complete block design with two factors (mungbean genotypes and salinity levels) and triplicates. Observation variables were germination percentage, vigor index, germination rate, hypocotyls length, epicotyls length, root length, number of root, seedling fresh weight, and seedling dry weight. The result showed that increasing level of salinity concentration inhibited the speed of germination, germination percentage, vigor index, normal seedling fresh weight, and number of lateral roots. Murai and Vima 1 were identified as tolerant genotypes, while Vima-2 and MLGV 0180 were identified as salinity sensitive genotypes at seedling stage. Currently, mungbean varieties with special characters, such as saline-tolerant is not yet available. The availability of saline-tolerant variety of mungbean is a cheaper and easier technology for farmers to anticipate the expansion of the saline area. The tolerant genotypes may be further tested at the later stage to obtain promising genotype tolerant to salinity that effectively assist mungbean breeding program.

Sign in / Sign up

Export Citation Format

Share Document