Assessment of Morphological and Molecular Genetic Diversity in Pigeonpea [Cajanus cajan (L.) Millspaugh]

2021 ◽  
Author(s):  
Tuntun Naing ◽  
S.K. Verma ◽  
Harikant Yadav ◽  
Charupriya Chauhan ◽  
Ashish Gautam ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Block Design ◽  
Molecular Genetic ◽  
Germplasm Conservation ◽  
Morphological Diversity ◽  
Sufficient Information ◽  
Additional Information ◽  
Molecular Genetic Diversity ◽  
Morphological Studies

Background: The estimation of genetic diversity in pigeonpea is crucial for desiging breeding programmes and germplasm conservation. Morphological studies alone do not provide sufficient information to understand genetic diversity. Molecular analysis using SSRs can provide additional information on genetic diversity that can be used for selection of diverse parents. Methods: The experimental material for present study consisted of 50 diverse genotypes of pigeonpea. The fifty genotypes were planted in Randomized Complete Block Design consisted of three replications during the kharif 2020-21. The estimation of morphological genetic diversity was done by Mahalanobis D2 statistics. The molecular genetic diversity analysis was done by using 30 molecular markers in same genotypes. Result: The assessment of morphological diversity revealed that the fifty pigeonpea genotypes were grouped into four different clusters with cluster I as the largest cluster (33 genotypes). The molecular markers differentiated the 50 genotypes in five major clusters with cluster II as the largest cluster (24 genotypes). The results of present study suggested that morphological and molecular diversity in pigeonpea is different. On the basis of genetic distance, the genotypes RVSA 2014-1 and PA 406 were found most genetically distant and may be used in hybridization programme to create diverse progenies.

scholarly journals Analysis of Morphological and Molecular Genetic Diversity of Salicornia Iranica Akhani Wildlife Populations around Urmia Lake, Iran

2021 ◽  
Author(s):  
Mohammad Aghaei ◽  
Abbas Hassani ◽  
Hossein Nazemiyeh ◽  
Babak Abdollahi Mandoulakani ◽  
Mohammad Saadatian
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Morphological Traits ◽  
Molecular Genetic ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Issr Markers ◽  
Shannon Index ◽  
Urmia Lake ◽  
Molecular Genetic Diversity

Abstract Salicornia is a halophyte plant capable of being irrigated with seawater, which can be used as an alternative food. Given this, it is necessary to study the potentials of this plant's morphological diversity in the natural environment. In this study, 33 wild populations of Salicornia were collected from different geographical areas around Urmia Lake during the flowering stage, and some morphological traits and 25 ISSR loci of the plant were measured. Based on morphological traits and the cluster analysis, Salicornia populations were divided into four groups. Overall, the high percentage of polymorphic gene loci (65.69%), the average number of effective alleles per gene locus (1.63), and the Shannon data index (0.540) indicate that ISSR markers can be used in Identify genetic diversity to be used. Molecular data cluster analysis divided the studied populations into two main groups, which included 12.12% and 87.88% of the populations, respectively. Based on the effective analysis of the population's genetic structure and the precise classification of individuals into suitable sub-populations, the value of K = 2 was calculated. The research findings indicated that markers UBC823, B, A7, and K, and with the Shannon index, effective allele, and large heterozygosity values are markers with the highest effectiveness compared to other markers utilized, and they are used better than other compounds in genetic distance. The findings of this study will aid in parental selection studies for breeding programs of salicornia in future.

scholarly journals Molecular genetic diversity assessment of Citrus species grown in Iran revealed by SSR, ISSR and CAPS molecular markers

2017 ◽  
Vol 2 (8) ◽  
pp. 22
Author(s):  
Ata Allah Sharafi ◽  
Asad Asadi Abkenar ◽  
Ali Sharafi
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Molecular Genetic ◽  
Citrus Species ◽  
Molecular Genetic Diversity ◽  
Diversity Assessment

En este estudio, se analizó la diversidad genética en 19 cultivares de cítricos mediante la repetición de secuencia simple (SSR), la repetición de secuencia inter-simple (ISSR) y los marcadores de secuencia polimórfica amplificada segmentada (CAPS). Se utilizaron nueve cebadores para SSR, nueve cebadores ISSR y dos cebadores para CAPS para la puntuación del alelo. Se analizaron una región de ADN de cloroplasto (rbcL-ORF106) y una región de ADN mitocondrial (18S-5S) usando marcador de secuencia polimórfica amplificada escindida (CAPS) en 19 accesiones de cítricos cultivadas en Irán. En total, se detectaron 45 genes SSR y 131 ISSR polimórficos alelos y organelos del genoma del árbol. El análisis de conglomerados de los datos SSR y ISSR se realizó utilizando el método UPGMA y se basó en el coeficiente de Jaccard. El resultado de esta investigación mostró que los cebadores SSR e ISSR eran altamente informativos y eficientes para detectar la variabilidad genética y las relaciones de las accesiones de los cítricos. Y el análisis de marcadores de CAPS Los resultados mostraron que Bakraee y uno de tipo off cal mexicana tenían un patrón de bandas similar al Clementine Mandarin, mientras que Pummelo se consideraba como padre materno de otros genotipos estudiados Citron considerado como padre padre mostró un patrón de bandas definido entre 19 genotipos estudiados que confirmó Citoplasma herencia de orgánulos celulares de la madre.  

Study on molecular genetic diversity of native duck breeds in China

2006 ◽  
Vol 62 (04) ◽  
pp. 603-611
Author(s):  
H. LI ◽  
N. YANG ◽  
K. CHEN ◽  
G. CHEN ◽  
Q. TANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity

Study of molecular genetic diversity and evolutionary history of medicinally important endangered genus Chlorophytum using DNA barcodes

2016 ◽  
Vol 65 ◽  
pp. 245-252 ◽  
Author(s):  
Swapnil M. Patil ◽  
Niraj R. Rane ◽  
Avinash A. Adsul ◽  
Avinash R. Gholave ◽  
Shrirang R. Yadav ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Evolutionary History ◽  
Molecular Genetic ◽  
Dna Barcodes ◽  
Molecular Genetic Diversity ◽  
History Of

scholarly journals Molecular genetic diversity and maternal origin of Chinese black-bone chicken breeds

2014 ◽  
Vol 13 (2) ◽  
pp. 3275-3282 ◽  
Author(s):  
W.Q. Zhu ◽  
H.F. Li ◽  
J.Y. Wang ◽  
J.T. Shu ◽  
C.H. Zhu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Maternal Origin ◽  
Molecular Genetic Diversity ◽  
Chicken Breeds
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