Salicylic Acid Induced Resistance against Mungbean Yellow Mosaic Virus (MYMV) and Enhanced Seed Yield in Resistant and Susceptible Urdbean [Vigna mungo (L.) Heper] Genotypes

2021 ◽  
Author(s):  
Sangita Sahni ◽  
Bishun Deo Prasad
Keyword(s):  
Salicylic Acid ◽  
Antioxidant Enzymes ◽  
Disease Resistance ◽  
Mosaic Virus ◽  
Seed Yield ◽  
Management Strategies ◽  
Field Conditions ◽  
Yellow Mosaic Virus ◽  
Long Period ◽  
Mungbean Yellow Mosaic Virus

Background: Urdbean’s low productivity is largely due to its susceptibility against whitefly-transmitted mungbean yellow mosaic virus (MYMV) disease. The effect of Salicylic acid (SA) on MYMV disease resistance and its impact on seed yield under field conditions on diverse genotypes is largely unknown. Therefore, in present investigation, we have analysed the effect of SA on induction of antioxidant enzymes leading to MYMV resistance and enhanced seed yield in urdbean genotypes. Methods: Different concentrations of SA were sprayed on 3 week-old susceptible urdbean genotype (LBG 623) and induction of antioxidant enzymes was analysed. A pot experiment was conducted to see the effect of SA on initial induction of antioxidant enzymes maintained over long period of time in 39 urdbean genotypes. Under field conditions, the effect of SA treatment on MYMV disease resistance and seed yield was assessed. Result: Rise in antioxidant enzyme production was observed in SA treated urdbean plants challenged with MYMV. The field experiment revealed that exogenous SA application significantly reduced MYMV incidence and increased seed yield in all 39 urdbean genotypes tested. The ability to confer MYMV resistance along with the increase in seed yield suggests the incorporation of SA in effective MYMV management strategies in urdbean.

Virus Vector Relationship of Yellow Mosaic Virus and Whitefly, Bemisia tabaci (Gennadius) in Soybean

2021 ◽  
Author(s):  
M. Swathi ◽  
Neeta Gaur ◽  
Kamendra Singh
Keyword(s):  
Mosaic Virus ◽  
Virus Disease ◽  
Management Strategies ◽  
Virus Transmission ◽  
Virus Vector ◽  
Yellow Mosaic Virus ◽  
Starvation Period ◽  
Mungbean Yellow Mosaic Virus ◽  
Biological Relationship ◽  
Relationship Of

Background: Whitefly is one of the most destructive sucking pest in the tropical and subtropical regions of the world and causing significant crop losses directly by sucking sap from the plants and indirectly through the transmission of viral diseases specifically caused by the genus Begomovirus. The Begomovirus species viz., Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) are causing yellow mosaic virus disease in soybean, which is transmitted by whiteflies. The disease accounts to 30-70 per cent yield loss and increases up to 80 - 100 per cent during severe incidence. Hence, there is a need for development of integrated pest management strategies against disease and whiteflies, for this the knowledge on virus-vector relationship is required. But, the studies on biological relationship of yellow mosaic virus disease and whitefly in soybean are scarce. At this juncture, considering the importance of disease in soybean, the present investigation was carried out to know the virus -vector relationship of the YMV and whitefly in soybean.Methods: The experiment on virus-vector relationship of yellow mosaic virus and whitefly in soybean was conducted at Department of Entomology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand during 2016-17. The data on number of whiteflies per plant, acquisition and inoculation access feeding period and pre and post starvation period required for effective transmission of virus was recorded.Result: A single viruliferous whitefly was able to transmit virus and ten viruliferous whiteflies per plant were required for cent per cent transmission of virus. The minimum acquisition access and inoculation access feeding periods required for virus transmission was 0.25h (15 min) each; while the 100 per cent virus transmission was recorded with acquisition and inoculation period of 12h, each. The per cent transmission was increased with the increase of acquisition and inoculation periods. The rate of transmission was positively correlated with pre-acquisition starvation period and negatively correlated with post- acquisition starvation period.

scholarly journals Screening of Urdbean Germplasm for High Seed Yield Coupled with Resistance to Mungbean yellow mosaic virus (MYMV)

2020 ◽  
pp. 100-106 ◽  
Author(s):  
Sunita Kumari ◽  
Sangita Sahni ◽  
Bishun Deo Prasad
Keyword(s):  
Mosaic Virus ◽  
Seed Yield ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Sources Of Resistance ◽  
Production Constraints ◽  
Mungbean Yellow Mosaic Virus ◽  
Disease Free ◽  
High Seed Yield

Yellow mosaic disease (YMD) caused by Mungbean yellow mosaic virus (MYMV) is one of the most destructive biotic production constraints in urdbean. Development and introduction of resistant cultivars with high seed yield are considered as the most economical and eco-friendly option to manage YMD, for which availability of stable sources of resistance with high seed yield is a pre-requisite. A set of one hundred twenty eight genotypes of urdbean including a susceptible check were evaluated against MYMV in the field for two consecutive years during summer and kharif 2015-2016 under natural condition of disease incidence. There was considerable variation among the genotypes with respect to disease reaction. Out of 128 genotypes tested, only five genotype namely KU 96-3, NDU 12-1,  NIRB 002,  NIRB 003 & NIRB 004 were found to be disease free, nineteen genotypes (IPU 10-23,  IPU 11-01, KPU 34, KUG 540,  KUG 586, Mash-338, NDU 12-2, NDU 12-300, NDUZ 14-21,OBG 35,  PU 09-35, Shekhar 3,  UH 07-06, Uttara, VBG 10-008, VBG 11-053,VBN (BG) 3, VBN 6 & Vijay) found to be highly resistant and twenty two genotypes (IGKU 02-1, Kopergaon,  KPU 12, KPU 13, KPU 14, KPU 16, KPU 33, KPU 7, KPU 8, KU 363,  NDU 11-01,Palampur 93, Pant U 19,  PU 08-05, PU 20, PU 22, RUG-44, Sekhar 2 , TU 67, UG 218, VBG 09-005 & WBU 108) showed highly resistance or resistance consistently in both the seasons. However, eight genotypes i.e. Uttara, PU-31, KU-363, KUG 540, UH 07-06, KUG 503, WBU 108 & Shekhar 33 were found to be superior for seed yield as well as resistant to MYMV. Among these lines, UH 07-06 and KUG 503 give highest seed yield in Kharif and summer.

Inheritance of mungbean yellow mosaic virus (MYMV) disease resistance in mungbean under natural infection conditions

2020 ◽  
Vol 18 (3) ◽  
pp. 196-199
Author(s):  
G. Basanagouda ◽  
S. Ramesh ◽  
N. Nagaraju ◽  
Nagaraj ◽  
A.S. Padmaja
Keyword(s):  
Disease Resistance ◽  
Mosaic Virus ◽  
Mode Of Action ◽  
Natural Infection ◽  
Yellow Mosaic Virus ◽  
Susceptible Genotype ◽  
Resistant Cultivars ◽  
Mungbean Yellow Mosaic Virus ◽  
Recessive Genes ◽  
Resistant Genotypes

AbstractMungbean yellow mosaic virus (MYMV) disease is one of the most devastating biotic constraints of mungbean production in India. Dependable knowledge on the number and mode of action of genes controlling resistance to MYMV disease is one of the keys to develop resistant cultivars. The F1s of four crosses derived from four MYMV resistant genotypes × one highly susceptible genotype, their parents, F2s and F3s along with a susceptible check were screened for responses to MYMV disease following the infector-row technique under natural infection conditions. A good fit of F2 population segregation to the hypothesized ratio of 15 susceptible:1 resistant and that of F3 population segregation to the expected ratio of 55 susceptible:9 resistant at 55 days after planting confirmed the involvement of two recessive genes in imparting resistance to MYMV disease.

scholarly journals Inheritance and Gene Effect of Resistance to Mungbean Yellow Mosaic Virus (MYMV) in Mungbean [Vigna radiata (L.) Wilczek]

2020 ◽  
Author(s):  
K.S. Win ◽  
S. Win ◽  
T.M. Htun ◽  
N.K.K. Win ◽  
K.S. Oo
Keyword(s):  
Disease Resistance ◽  
Mosaic Virus ◽  
Recessive Gene ◽  
Yellow Mosaic Virus ◽  
Inheritance Pattern ◽  
Gene Effects ◽  
Mungbean Yellow Mosaic Virus ◽  
Production Knowledge ◽  
Resistant Genotypes ◽  
Mode Of Inheritance

Background: Mungbean Yellow Mosaic Virus is one of the major constraints in mungbean production. Knowledge of mode of inheritance and gene effects of MYMV resistance is very useful and effective for the development of genotypes resistant to disease or incorporation of resistance into the desirable promising genotypes whichlack of disease resistance. Methods: In order to estimate inheritance pattern of MYMV disease resistance in mungbean, the study was conducted in summer season (2019) under natural condition. Six generations such as Pl, P2, Fl, BC1, BC2 and F2 of six combinations [two resistant genotypes (7639 and 10266) and three susceptible genotypes (7621, 10257 and R-021018)] were studied inheritance pattern of resistance to MYMV in segregation population. Result: Based on the result from mode of inheritance pattern of MYMV resistance, it can be concluded that single recessive gene is controlled the resistance of MYMV and susceptible behavior indicated as dominant over resistant. Additive gene action was the major role for the selection of MYMV resistance. Some differences in the expression of gene contributing for MYMV resistance from others findings might be attributed due to the sources of resistant genotypes which have different nature of resistant gene used in this investigation. 

Identification and Validation of Quantitative Trait Loci of Mungbean Yellow Mosaic Virus Disease Resistance in Blackgram [Vigna mungo (L). Hepper]

2021 ◽  
Author(s):  
K. Vadivel ◽  
N. Manivannan ◽  
A. Mahalingam ◽  
V.K. Satya ◽  
C. Vanniarajan ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Quantitative Trait Loci ◽  
Mosaic Virus ◽  
Phenotypic Variation ◽  
Quantitative Trait ◽  
Tamil Nadu ◽  
Virus Disease ◽  
Yellow Mosaic Virus ◽  
Disease Score ◽  
Mungbean Yellow Mosaic Virus

Background: Blackgram [Vigna mungo (L.) Hepper] is an important food legume crop of India. Mungbean yellow mosaic virus (MYMV) disease is the major problem in blackgram. The disease can reduce seed yield upto 100% or even kill a plant infected at an early vegetative stage. The most effective way to prevent the occurrence of this disease is to develop genetically resistant cultivars of blackgram. However, a major difficulty in breeding MYMV disease resistant in blackgram is field screening for the virus disease. Hence identification of QTL followed by Marker-assisted selection (MAS) is highly useful for genetic improvement of crops. With this background, a study was made for identification as well as validation of quantitative trait loci (QTL) for MYMV disease resistance in blackgram.Methods: A total of 112 F2:3 lines were evaluated for MYMV disease resistance along with parents viz., MDU 1 (MYMV disease susceptible) and Mash 1008 (MYMV disease resistant) at the National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, Tamil Nadu during July-September 2018 under Augmented Design in the field. Each line was sown in one row of 3 m in length with a spacing of 30 cm as between row and 10 cm as within row. Susceptible genotypes CO 5 and MDU 1 were sown as disease spreader rows after every eight rows and also around the plots. The MYMV disease score was recorded on 60 DAS, by using phenotype rating scale from 1 (resistant) to 9 (highly susceptible), as suggested by Singh et al. (1995). The mean of each progeny was calculated and used as phenotypic data. A total of 525 SSR primers were used to test polymorphism between parents MDU 1 and Mash 1008. Genotyping was carried out for 112 F2:3 RILs of the cross MDU 1 x Mash 1008 with 35 polymorphic SSR markers. Linkage and QTL analyses were performed using QTL IciMapping (version 4.1.0.0) (Wang et al. 2016) and QGene 4.4.0 (Joehanes and Nelson 2008) respectively. Two mapping populations MDU 1 x Mash 114 and CO 5 x VBN 6 in F2:3 and F2 generations respectively were used in this study to validate the identified QTL regions.Result: QTL study indicated the presence of two major QTLs for MYMV disease score in LG 2 and LG 10 at 60 DAS with 20.90 and 24.90% of phenotypic variation respectively. Validation of these QTLs in two other mapping population indicated that QTL on LG 10 was validated with high phenotypic variation of 45.40-46.00%. Hence it may conclude that qmymv10_60 may be utilized for MAS/MABC with assured improvement on MYMV disease resistance in blackgram.

Inheritance Pattern of Mungbean Yellow Mosaic Virus (MYMV) Disease Resistance in Blackgram [Vigna mungo (L.) Hepper]

2021 ◽  
Author(s):  
K. Vadivel ◽  
N. Manivannan ◽  
A. Mahalingam ◽  
V.K. Satya ◽  
S. Ragul
Keyword(s):  
Disease Resistance ◽  
Mosaic Virus ◽  
Tamil Nadu ◽  
Gene Action ◽  
Disease Incidence ◽  
Yellow Mosaic Virus ◽  
Inheritance Pattern ◽  
Complementary Gene ◽  
Mungbean Yellow Mosaic Virus ◽  
Complementary Gene Action

Background: Mungbean yellow mosaic virus (MYMV) disease is the most destructive disease in blackgram. Development of MYMV resistant varieties is one of the best possible solutions to avoid the yield reduction in blackgram. There are conflicting reports on the genetics of resistance to MYMV disease claiming that it is controlled by both dominant and recessive genes. Hence the present study was aimed to understand the inheritance pattern of the MYMV disease resistance in eight crosses of blackgram.Methods: Parents, F1 and F2 generation of eight cross combinations were raised during July - Sep, 2018 at National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, Tamil Nadu. An infector row of CO 5 was raised to intensify the MYMV disease pressure after every eight rows. Based on disease incidence on 60th day after sowing, two phenotypic classes were formed among F2 plants with the scales of (1 to 3) as resistant phenotype and (4 to 9) as susceptible phenotype. The goodness of fit to Mendelian segregation ratio for MYMV disease resistance in the segregating population was tested by Chi square test (Stansfield, 1991).Result: The MYMV disease incidence was tri-genically controlled with inhibitory gene action in four crosses viz., MDU 1 x Mash 114, CO5 x Mash 114, MDU 1 x VBN 6 and CO 5 x VBN 6. Complementary gene action with two genes was observed in four crosses viz., MDU 1 x Mash 1008, CO 5 x Mash 1008, MDU 1 x VBN 8 and CO 5 x VBN 8. Differences in number of genes were observed due to the presence of recessive inhibitory gene in both male and female parents of the crosses which had complementary gene action for MYMV disease. The putative gene symbols assigned for the six genotypes viz., S1S1S2S2ii (MDU 1 and CO 5), s1s1s2s2II (Mash 114 and VBN 6) and s1s1s2s2ii (Mash 1008 and VBN 8), respectively. 

Sign in / Sign up

Export Citation Format

Share Document