Unravelling the Role of Glomus mosseae in the Alleviation of Salinity Stress in Mungbean [Vigna radiata (L.) Wilczek]

2021 ◽  
Author(s):  
Moushree Sarkar ◽  
Sabyasachi Kundagrami
Keyword(s):  
Nutrient Uptake ◽  
Salinity Stress ◽  
Glomus Mosseae ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Fold Increase ◽  
Remarkable Recovery ◽  
Efficient Protection ◽  
Use Efficiency ◽  
The Impact

Background: Salinity stress remains a chronic threat to pulses productivity in India. Arbuscular mycorrhizal (AM) fungi play a major role which influences plant growth, nutrient uptake and contributes to ecosystem processes under salt stress. The present study aims, to demonstrate the impact of Glomus mosseae (Gm), on physio-biochemical attributes of mungbean exposed to salinity. Methods: Two highly tolerant, two moderately susceptible and two highly susceptible mungbean lines were subjected to salinity stress alone and in presence of Gm under greenhouse. Result: Results revealed that Gm alleviates the salinity stress related alterations by improving the nutrient uptake and by balancing the ratio between K:Na, which impact directly the osmoregulation of the plants. Mycorrhiza inoculation also increased the proline content (23%), water-use efficiency (38%) and activity of different antioxidant enzymes in a significant manner providing efficient protection against salinity stress. All these positive impacts of Gm were duly reflected in a significant increase in grain yield (more than 2 fold increase) in mungbean. Interestingly, salt-induced retarded growth and decline in other biochemical parameters in susceptible lines recorded remarkable recovery following Gm inoculation.

scholarly journals Tracking of Zinc Ferrite Nanoparticle Effects on Pea (Pisum sativum L.) Plant Growth, Pigments, Mineral Content and Arbuscular Mycorrhizal Colonization

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 583
Author(s):  
Reda E. Abdelhameed ◽  
Nagwa I. Abu-Elsaad ◽  
Arafat Abdel Hamed Abdel Latef ◽  
Rabab A. Metwally
Keyword(s):  
Pisum Sativum ◽  
Plant Growth ◽  
Zinc Ferrite ◽  
Crop Improvement ◽  
Nanocrystalline Structure ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Transmission Electron ◽  
Agricultural Applications ◽  
The Impact

Important gaps in knowledge remain regarding the potential of nanoparticles (NPs) for plants, particularly the existence of helpful microorganisms, for instance, arbuscular mycorrhizal (AM) fungi present in the soil. Hence, more profound studies are required to distinguish the impact of NPs on plant growth inoculated with AM fungi and their role in NP uptake to develop smart nanotechnology implementations in crop improvement. Zinc ferrite (ZnFe2O4) NPs are prepared via the citrate technique and defined by X-ray diffraction (XRD) as well as transmission electron microscopy for several physical properties. The analysis of the XRD pattern confirmed the creation of a nanocrystalline structure with a crystallite size equal to 25.4 nm. The effects of ZnFe2O4 NP on AM fungi, growth and pigment content as well as nutrient uptake of pea (Pisum sativum) plants were assessed. ZnFe2O4 NP application caused a slight decrease in root colonization. However, its application showed an augmentation of 74.36% and 91.89% in AM pea plant shoots and roots’ fresh weights, respectively, compared to the control. Moreover, the synthesized ZnFe2O4 NP uptake by plant roots and their contents were enhanced by AM fungi. These findings suggest the safe use of ZnFe2O4 NPs in nano-agricultural applications for plant development with AM fungi.

scholarly journals Effects of Temperature and Grafting on Yield, Nutrient Uptake, and Water Use Efficiency of a Hydroponic Sweet Pepper Crop

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 110 ◽  
Author(s):  
Andreas Ropokis ◽  
Georgia Ntatsi ◽  
Constantinos Kittas ◽  
Nikolaos Katsoulas ◽  
Dimitrios Savvas
Keyword(s):  
Water Use Efficiency ◽  
Nutrient Uptake ◽  
Water Use ◽  
Total Yield ◽  
Sweet Pepper ◽  
Winter Climate ◽  
Mild Winter ◽  
Yield Increase ◽  
Use Efficiency ◽  
The Impact

In areas characterized by mild winter climate, pepper is frequently cultivated in unheated greenhouses in which the temperature during the winter may drop to suboptimal levels. Under low temperature (LT) conditions, the uptake of nutrients may be altered in a different manner than that of the water and thus their uptake ratio, known as uptake concentration, may be different than in greenhouses with standard temperature (ST) conditions. In the present study, pepper plants of the cultivars “Sammy” and “Orangery”, self-grafted or grafted onto two commercial rootstocks (“Robusto” and “Terrano”), were cultivated in a greenhouse under either ST or LT temperature conditions. The aim of the study was to test the impact of grafting and greenhouse temperature on total yield, water use efficiency, and nutrient uptake. The LT regime reduced the yield by about 50% in “Sammy” and 33% in “Orangery”, irrespective of the grafting combination. Grafting of “Sammy” onto both “Robusto” and “Terrano” increased the total fruit yield by 39% and 34% compared with the self-grafted control, while grafting of “Orangery” increased the yield only when the rootstock was “Terrano”. The yield increase resulted exclusively from enhancement of the fruit number per plant. Both the water consumption and the water use efficiency were negatively affected by the LT regime, however the temperature effect interacted with the rootstock/scion combination. The LT increased the uptake concentrations (UC) of K, Ca, Mg, N, and Mn, while it decreased strongly that of P and slightly the UC of Fe and Zn. The UC of K and Mg were influenced by the rootstock/scion combination, however this effect interacted with the temperature regime. In contrast, the Ca, N, and P concentrations were not influenced by the grafting combination. The results of the present study show that the impact of grafting on yield and nutrient uptake in pepper depend not merely on the rootstock genotype, however on the rootstock/scion combination.

scholarly journals Detection of Arbuscular Mycorrhizal Fungi in the Roots of Strawberry Plants Fertilized with Organic Bioproducts

2012 ◽  
Vol 77 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Anna Lisek ◽  
Lidia Sas Paszt ◽  
Beata Sumorok
Keyword(s):  
Plant Growth ◽  
Arbuscular Mycorrhizal Fungi ◽  
Nested Pcr ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Universal Primers ◽  
Mineral Fertilizers ◽  
Reaction Products ◽  
The Impact

Summary In organic farming, mineral fertilizers are replaced by various preparations to stimulate plant growth and development. Introduction of new biopreparations into horticultural production requires an assessment of their effects on the growth and yielding of plants. Among the important indicators of the impact on plants of beneficial microorganisms contained in bioproducts is determination of their effectiveness in stimulating the growth and yielding of plants. Moreover, confirmation of the presence of arbuscular mycorrhizal (AM) fungi in the roots and plant growth promoting rhizobacteria (PGPR) in the rhizosphere is also necessary. In addition to conventional methods, molecular biology techniques are increasingly used to allow detection and identification of AM fungi in plant roots. The aim of this study was identification and initial taxonomic classification of AM fungi in the roots of ‘Elkat’ strawberry plants fertilized with various biopreparations using the technique of nested PCR. Tests were performed on DNA obtained from the roots of ‘Elkat’ strawberry plants: not fertilized, treated with 10 different biopreparations, or fertilized with NPK. Amplification of the large subunit of ribosomal gene (LSU rDNA) was carried out using universal primers, and then, in the nested PCR reaction, primers specific for the fungi of the genera Glomus, Acaulospora, and Scutellospora were used. Colonization of strawberry roots by arbuscular mycorrhizal fungi was determined on the basis of the presence of DNA fragments of a size corresponding to the types of the fungi tested for. As a result of the analyses, the most reaction products characterizing AM fungi were found in the roots of plants treated with the preparation Florovit Eko. The least fragments characteristic of AM fungi were detected in the roots of plants fertilized with NPK, which confirms the negative impact of mineral fertilizers on the occurrence of mycorrhizal fungi in the roots of strawberry plants. The roots of plants fertilized with Tytanit differed from the control plants by the presence of one of the clusters of fungi of the genus Glomus and by the absence of a cluster of fungi of the genus Scutellospora. In the roots of plants treated with other biopreparations there were reaction products indicating the presence of fungi of the genera Glomus, Scutellospora and Acaulospora, like in the roots of the control plants. The results will be used to assess the suitability of microbiologically enriched biopreparations in horticultural production.

scholarly journals Tillage, Glyphosate and Beneficial Arbuscular Mycorrhizal Fungi: Optimising Crop Management for Plant–Fungal Symbiosis

Agriculture ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 520 ◽  
Author(s):  
Thomas I. Wilkes ◽  
Douglas J. Warner ◽  
Keith G. Davies ◽  
Veronica Edmonds-Brown
Keyword(s):  
Mycorrhizal Fungi ◽  
Fungal Biomass ◽  
Negative Impact ◽  
Cropping Systems ◽  
Fungal Growth ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Soil Microbiome ◽  
Epsp Synthase ◽  
The Impact

Zero till cropping systems typically apply broad-spectrum herbicides such as glyphosate as an alternative weed control strategy to the physical inversion of the soil provided by cultivation. Glyphosate targets 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in plants. There is growing evidence that this may have a detrimental impact on non-target organisms such as those present in the soil microbiome. Species of commercial importance, such as arbuscular mycorrhizal (AM) fungi that form a symbiotic relationship with plant roots are an important example. This study investigates the impact of soil cultivation and glyphosate application associated with conventional tillage (CT) and zero tillage (ZT) respectively on AM fungi populations under field and glasshouse conditions. Topsoil (<10 cm) was extracted from CT and ZT fields cropped with winter wheat, plus non-cropped control plots within the same field boundary, throughout the cropping year. Glyphosate was applied in glasshouse experiments at rates between 0 and 350 g L−1. Ergosterol, an indicator of fungal biomass, was measured using high performance liquid chromatography before and after glyphosate application. Fungal root arbuscules, an indicator of AM fungi–root symbiosis, were quantified from the roots of wheat plants. Under glasshouse conditions root arbuscules were consistently higher in wheat grown in ZT field extracted soils (P = 0.01) compared to CT. Glyphosate application however inhibited fungal biomass in both the ZT (P < 0.00001) and CT (P < 0.001) treatments. In the absence of glyphosate, the number of stained root arbuscules increased significantly. Ergosterol levels, used as a proxy for fungal biomass, remained lower in the soil post glyphosate application. The results suggest that CT has a greater negative impact on AM fungal growth than ZT and glyphosate, but that glyphosate is also detrimental to AM fungal growth and hinders subsequent population recovery.

Differential contribution of arbuscular mycorrhizal fungi to plant nitrate uptake (15N) under increasing N supply to the soil

2001 ◽  
Vol 79 (10) ◽  
pp. 1175-1180 ◽  
Author(s):  
R Azcón ◽  
J M Ruiz-Lozano ◽  
R Rodríguez
Keyword(s):  
Mycorrhizal Fungi ◽  
Nitrate Uptake ◽  
Glomus Mosseae ◽  
Arbuscular Mycorrhizae ◽  
Nitrogen Nutrition ◽  
Nitrate Assimilation ◽  
Growth Period ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Mycorrhizal Symbiosis

The objective of this study was to determine how the uptake and transport of nitrate by two species of arbuscular mycorrhizal (AM) fungi is affected by its concentration in the medium and by the age of the AM symbiosis. Tracer amounts of15N nitrate were applied at two plant growth periods to mycorrhizal or nonmycorrhizal lettuce plants, which had been grown in soil supplied with nitrate to provide a total of 84, 168, or 252 mg N/kg. At both injection times, Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe and Glomus fasciculatum (Thaxter sensu Gerd.) Gerd. and Trappe reached the highest values of nitrogen derived from the fertilizer (NdfF) at 84 mg N/kg. Glomus mosseae also reached the highest values of labeled fertilizer N utilization at 84 mg N/kg, whereas G. fasciculatum reached the highest values at 168 mg N/kg in the medium. The highest N level in the medium (252 mg N/kg) had a negative effect on % NdfF and % labeled fertilizer utilization for all mycorrhizal plants. Regarding the time of15N fertilizer application, G. fasciculatum-colonized plants had a minimum change in % NdfF and % labeled fertilizer utilization during the growth period (60 days application vs. 30 days application). In contrast, G. mosseae-colonized plants growing at 168 mg N/kg in the medium, decreased these two values in the latest application. The present results confirm that mycorrhizal symbiosis may be particularly important for nitrogen nutrition in plants growing in neutral-alkaline soils.Key words: arbuscular mycorrhizae, nitrate assimilation, nitrate uptake,15N-labeled fertilizer.

Contribution of arbuscular mycorrhizal (AM) fungi and hedgerow trees to the yield and nutrient uptake of cassava in an alley-cropping system

1998 ◽  
Vol 131 (1) ◽  
pp. 79-85 ◽  
Author(s):  
O. FAGBOLA ◽  
O. OSONUBI ◽  
K. MULONGOY
Keyword(s):  
Nutrient Uptake ◽  
Alley Cropping ◽  
Cropping System ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Weight ◽  
P Uptake ◽  
Root Competition ◽  
Woody Legumes ◽  
Woody Legume

A field trial on alley-cropping was conducted at the University of Ibadan research farm in the 1990/91 cropping season to assess the contributions of arbuscular mycorrhizal (AM) fungi and hedgerow woody legumes to the yield and nutrient uptake of cassava (Manihot esculenta Crantz) as an intercrop in an infertile soil. The trial also investigated the influence of AM fungi on the interplanting of a non-nodulating woody legume Senna siamea (syn. Cassia siamea) with a nodulating woody legume (Leucaena leucocephala).AM contributions to cassava were greater than the hedgerow contributions, which demonstrated that AM associations are an essential component in the nutrition of cassava. In contrast to cassava, AM inoculation only influenced the leaf dry weight and uptake of nutrients of non-interplanted woody legumes but not the above-ground biomass and P uptake of interplanted woody legumes. However, non-inoculated interplanted Leucaena benefited more from indigenous AM fungi than the competing Senna. The negative contributions to the nutrient uptake (K, Ca and Mg) of cassava by hedgerows and the lack of response to AM inoculation in interplanted hedgerow woody legumes could be attributed to root competition among the different plant species growing in close proximity to each other. The present results show that cassava benefits more from AM association than Leucaena which in turn benefits more than Senna in an alley-cropping system.

scholarly journals A New Standard Area Diagram Set for Assessment of Severity of Soybean Rust Improves Accuracy of Estimates and Optimizes Resource Use

2020 ◽  
Author(s):  
Emerson Medeiros Del Ponte ◽  
Kaique Santos Alves
Keyword(s):  
Resource Use ◽  
Fold Increase ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Resource Use Efficiency ◽  
Severe Reduction ◽  
New Knowledge ◽  
Disease Reduction ◽  
Use Efficiency ◽  
The Impact

Soybean rust (SBR), caused by Phakopsora pachyrhizi, is the most important yield- damaging fungal disease of soybean due to severe reduction in healthy leaf area and acceleration of leaf fall. In experimental research, SBR severity is estimated visually aided/trained by a standard area diagram (SAD) developed and validated during the mid- 2000s (Old SAD). In this study, we propose a new SAD set for SBR with six true-colour diagrams following linear increments (c.15% increments) amended with four additional diagrams at low (&lt;10%) severities, totaling 10 diagrams (0.2%, 1%, 3%, 5%, 10%, 25%, 40%, 55%, 70%, and 84%). For evaluation, 37 raters were split into two groups. Each assessed severity in a 50-image sample (0.25% to 84%), first unaided and then using either the Old SAD or the New SAD. Accuracy, precision, and reliability of estimates improved significantly relative to unaided estimates only when aided by the New SAD (accuracy &gt;0.95). Low precision (&lt;0.78) and a trend of underestimation with an increase in severity were the main issues with the Old SAD, which did not differ from unaided estimates. Simulation to evaluate the impact of the errors by different methods on hypothesis tests, showed that the new SAD was more powerful for detecting the smallest difference in mean control (e.g., 70% vs. 65% disease reduction) than the Old SAD; the latter required a 2-fold increase in sample size to achieve the same power. There is a need to improve some SADs, taking advantage of new knowledge and technology to increase accuracy of the estimates, and to optimize both resource use efficiency and management decisions.

scholarly journals Effect of greenhouse microclimate and water quality on nutrient uptake in pepper hydroponic crop

2021 ◽  
Author(s):  
Ανδρέας Ροπόκης
Keyword(s):  
Water Use Efficiency ◽  
Nutrient Uptake ◽  
Water Use ◽  
Water Uptake ◽  
Irrigation Water ◽  
Sweet Pepper ◽  
Climatic Conditions ◽  
Fruit Yield ◽  
Use Efficiency ◽  
The Impact

The nutrient to water uptake ratios, henceforth termed “uptake concentrations” (UC), remain relatively constant over time under similar climatic conditions for a particular plant species and developmental stage. Under greenhouses with low temperature (LT) conditions, the uptake of nutrients may be altered in a different manner than that of the water and thus their UC may be different than in greenhouses with standard temperature (ST) conditions. In the Mediterranean regions, sweet pepper is frequently cultivated in unheated greenhouses in which the temperature during the winter may drop to suboptimal or even lower levels. In these areas, the available irrigation water frequently contains sodium chloride but also calcium bicarbonate, which at excessively high concentrations in closed hydroponic crops can impose Ca accumulation in the recycled NS and concomitantly negatively affect fruit yield and quality of the produce.Taking the above into consideration there were established three studies:In the first study, pepper plants of the cultivars ‘Sammy’ and ‘Orangery’, self-grafted or grafted onto two commercial rootstocks ('Robusto' and 'Terrano'), were cultivated in a greenhouse under either ST or LT conditions. The aim of the study was to test the impact of grafting and greenhouse temperature on total yield, water use efficiency and nutrient uptake. The LT regime reduced yield by about 50% in ‘Sammy’ and 33% in ‘Orangery’, irrespective of the grafting combination. Grafting of ‘Sammy’ onto both 'Robusto' and 'Terrano' increased the total fruit yield by 39% and 34% compared with the self-grafted control, while grafting of ‘Orangery’ increased yield only when the rootstock was ‘Terrano’. The yield increase resulted exclusively from enhancement of the fruit number per plant. Both the water consumption and the water use efficiency were suppressed by the LT regime but the temperature effect interacted with the rootstock/scion combination. The LT increased the UC of K, Ca, Mg, N, and Mn, while it decreased strongly that of P and slightly the UC of Fe, and Zn. The UC of K and Mg were influenced by the rootstock/scion combination but this effect interacted with the temperature regime. In contrast, the Ca, N, and P concentrations were not influenced by the grafting combination. The results of the present study show that the impact of grafting on yield and nutrient uptake in pepper depend not merely on the rootstock genotype but on the rootstock/scion combination.In the second study, mean UC of macro- and micronutrients were determined during five developmental stages in different pepper cultivars grown in a closed hydroponic system by measuring the water uptake and the nutrient removal from the RNS. The experiment was conducted in a Mediterranean environment and the tested cultivars were ‘Orangery’, ‘Bellisa’, ‘Sondela’, ‘Sammy’ self-grafted and ‘Sammy’ grafted onto the commercial rootstock `RS10'. ‘Sondela’ exhibited significantly higher ΝΟ3, Μg, Ca and B UC in comparison with all other cultivars, while Bellisa exhibited higher K UC. The UC of all nutrients were similar in the grafted and the non-grafted ‘Sammy’ plants. The UC of macronutrients estimated in the second study (mmol L-1) ranged from 2.4 to 3.7 for Ca, 1.0 to 1.5 for Mg, 6.2 to 9.0 for K, 11.7 to 13.7 for N, and 0.7 to 1.1 for P. The UC of N, K, Ca and Mg were appreciably higher than the corresponding values found under Dutch climatic conditions, while that of P was similar in both environments during the vegetative stage and higher thereafter. The UC of Fe, Zn and B tended to decrease with time, while that of Mn increased initially and subsequently decreased slightly during the reproductive developmental stage.In the third study, irrigation water containing 1.5, 3.0, 4.5 and 6.0 mM was used to prepare NS in a closed hydroponic crop of sweet pepper cultivated in RNS. The aim of the study was to determine maximum Ca levels that do not harm the crop and to simulate the pattern of Ca accumulation when the Ca concentration in the irrigation water is excessive. At 1.5 mM Ca, no Ca accumulation was observed in the RNS, while at 3.0, 4.5 and 6.0 mM the Ca concentration in the RNS, and concomitantly in the root environment, increased to 17, 28 and 37 mM, corresponding to 6.4, 9.0 and 10.8 dS m-1. The accumulation of Ca in the RNS affected both tissue nutrient concentrations and UC of Ca, S and Mg, but this was not the case for N and K. Growth, yield and plant water uptake were restricted at moderate and high external Ca levels. Our results showed that in soilless sweet pepper crops with zero discharge of fertigation effluents, the Ca concentration in the irrigation water should be lower than 3.0 mM to avoid yield restrictions due to salinity.

scholarly journals Evaluate the association of arbuscular mycorrhizal fungi in some medicinal plants grown in noyal river bed, tiruppur district, tamil nadu

2020 ◽  
Vol 7 (1) ◽  
pp. 54-62
Author(s):  
Venkatachalapathi A ◽  
Abdul Kaffoor H ◽  
Nagarajan N
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Tamil Nadu ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Qualitative Assessment ◽  
Polluted Soils ◽  
River Bed ◽  
Physico Chemical ◽  
The Impact

To evaluate the rhizosphere soils and ten medicinal herbs polluted soils were tested for the association of arbuscular mycorrhizal fungi and determined the impact of the physico chemical factors in relation to the quantitative and qualitative assessment of AM fungi in polluted soils. Forty species of AMF belonging to five genera such as Glomus, Acaulospora, Gigaspora, Sclerocystis and Scutellospora were recorded and identified. Glomus fistulosum was noticed as the moist dominant in the polluted. In the non-polluted soils,all the plant species were colonized with AM fungi. Where as in polluted soils, eight herb species only were colonized and the percentage of root colonization was less.

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