Identification of Source of Resistance to Horse Gram Yellow Mosaic Disease (HgYMD) in French Bean (Phaseolus vulgaris L.)

2021 ◽  
Author(s):  
Arindam Das ◽  
T.S. Aghora ◽  
M. Krishna Reddy ◽  
P. Nandeesha ◽  
R. Venugopalan
Keyword(s):  
Average Rate ◽  
Block Design ◽  
Economic Loss ◽  
French Bean ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Limiting Factor ◽  
Yellow Mosaic Disease ◽  
Horse Gram ◽  
Progress Curve

Background: Horse gram yellow mosaic disease (HgYMD) is one of the major limiting factor for legumes production in India as it can cause economic loss up to 100%. Till date, horse gram yellow mosaic virus was rare to infect french bean, but now-a-days, it has become the major causal agent for yellow mosaic epidemics in the southern part of India. Few bush type varieties, resistant to yellow mosaic disease are released but not specifically resistant to HgYMD. So, systemic breeding work has been initiated to identify the resistant source for HgYMD in french bean. Methods: Seventyone genotypes of french bean were screened in a randomized complete block design (RCBD) with three replications under natural epiphytotic as well as artificial condition during Summer, 2018 at ICAR-IIHR, Bengaluru, India. Result: The genotypes varied for percent disease index (PDI), area under disease progress curve (AUDPC) and the apparent rate of infection per unit time period (r). Two varieties i.e., Jade-5058 and Arka Arjun were showing less PDI of 13.33 and 14.44, least average rate of infection of 0.64 and 0.65 and lowest AUDPC value of 350.54 and 359.16 respectively, compared to other genotypes. Average PDI of Arka Arjun and Jade-5058 were 13.33 and 12.22 under artificial screening, which were much lesser than susceptible check Arka Komal (PDI 92.22) and Arka Anoop (PDI 90.00). Resistant genotypes identified from this experiment can be used for breeding HgYMD resistant french bean lines.

Coat protein and movement protein based characterization of Lima bean yellow mosaic virus disease in Karnataka (India)

2016 ◽  
Author(s):  
M. Bhagyashree ◽  
S. Basavaraj ◽  
H. A. Prameela ◽  
G. Jyoti ◽  
Manjunath S. Hurakadli ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Lima Bean ◽  
French Bean ◽  
Mosaic Disease ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nucleotide Identity ◽  
Viral Gene ◽  
Yellow Mosaic Disease ◽  
Pcr Product

Yellow mosaic disease caused by a Begomovirus has become an important production constraint in lima bean cultivation in Karnataka. The begomovirus causes Lima bean Yellow Mosaic Virus (LYMV), was characterized by sequencing the CP and MP genes. Distinct viral gene specific PCR product corresponding to CP and MP gene ~1000 bp and ~900 bp was obtained. The PCR product were sequenced and compared with the reference Begomoviruses sequences from the NCBI blast database. LYMV (GKVK isolate) shared highest nucleotide identity (98%) with Horsegram yellow mosaic virus (HgYMV) of French bean isolate (Accession No. AM932425.1). Phylogenetic analysis of CP and MP gene sequences of LYMV-GKVK isolate showed that LYMV-GKVK clustered with isolates of HgYMV Horsegram and French bean isolates of Tamil Nadu, Srilanka, Karnataka. Based on Nucleotide Identity begomovirus infecting lima bean at Bangalore is to be considered as strain of HgYMV. The disease was also successfully transmitted to French bean and Horsegram when experimentally transmitted using whitefly vector. Therefore, the begomovirus causing yellow mosaic disease of lima bean is considered as strain of HgYMV. Lima bean is infected by Mungbean Yellow Mosaic India Virus (MYMIV) according to earlier reports whereas our study the reports the infection of HgYMV on Lima bean from Karnataka. Thus it is concluded that Lima bean is infected by both MYMIV and HgYMV in different geographical locations.

​RCA-based Detection of Begomoviruses in Weed Ggenera Associated with Legumes in Southern Karnataka

2021 ◽  
Author(s):  
Sudeep Pandey ◽  
T.R. Girish ◽  
S. Basavaraj ◽  
A.S. Padmaja ◽  
N. Nagaraju
Keyword(s):  
Mosaic Virus ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Insect Vector ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Mungbean Yellow Mosaic Virus ◽  
Legume Crops

Background: Yellow mosaic disease (YMD) caused by begomoviruses transmitted through the insect vector Bemisia tabaci poses a serious threat to the production of legume crops. Methods: Season-long surveys were carried out for YMD occurrence in six different legume crops and associated natural weeds both symptomatic and asymptomatic across the districts of southern Karnataka, India. The samples were analyzed through RCA PCR using specific primer pairs. Result: Up to 94.1 per cent YMD incidence was recorded and nine weed species were commonly found associated with legume crops. The weeds viz., Ageratum conyzoides, Alternanthera sessilis, Commelina benghalensis and Euphorbia geniculata were abundantly found in the surveyed regions. The weeds were both symptomatic and asymptomatic. Rolling circle amplification coupled polymerase chain reaction method was employed to detect yellow mosaic virus in asymptomatic weeds. Phylogenetic analysis based on the sequences of PCR amplified products of weeds and symptomatic legumes revealed a close clustering of the weed samples with horsegram yellow mosaic virus, legume yellow mosaic virus and mungbean yellow mosaic virus. Overall, our data suggests the role of weed species associated with legume crops as alternative/collateral hosts of begomoviruses and their role in the epidemiology of yellow mosaic disease.

Survey for Yellow Mosaic Disease Occurring on Blackgram [Vigna mungo (L.) Hepper] and Weed Hosts in Four Major Blackgram Growing Districts of Andhra Pradesh and Characterization of Associated Viruses using Coat Protein Gene

2021 ◽  
Author(s):  
Vallabhaneni Tilak Chowdary ◽  
V. Manoj Kumar ◽  
P. Kishore Varma ◽  
B. Sreekanth ◽  
V. Srinivasa Rao
Keyword(s):  
Coat Protein ◽  
Andhra Pradesh ◽  
Disease Incidence ◽  
Pcr Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Weed Plants ◽  
Weed Hosts

Background: Yellow mosaic disease (YMD) caused by Yellow mosaic virus is one of the major constraints in the pulse production in Andhra Pradesh (A.P.) due to fast evolution of strains, like Mungbean yellow mosaic India virus (MYMIV). Keeping this in view, a survey was undertaken in the major blackgram growing districts of A.P. to know the YMD incidence in blackgram and weed hosts and were characterized based on genetic features by comparing with other YMV isolates from different hosts and locations across the world. Methods: Roving survey was conducted during rabi 2019-20 in major blackgram growing districts of A.P. viz., Krishna, Guntur, West Godavari and Prakasam districts for YMD incidence. Blackgram plants showing characteristic symptoms were collected as representative samples from each mandal along with the suspected weed plants and were subjected to amplification using coat protein (CP) specific primers followed by molecular characterization. Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny. Result: Out of the four districts surveyed, the highest disease incidence was recorded at Machavaram village of Prakasam district (43.22%), whereas least disease incidence was recorded at Chinaganjam village of Praksam district (2.4%). Six weeds viz., Ageratum conyzoides, Amaranthus viridis, Parthenium hysterophporus, Vigna trilobata, Abelmoscus moschatus, Desmodium laxiflorum have showed positive result in PCR amplification with MYMIV specific coat protein primers. Four isolates from blackgram samples and two from weed plants shared 94.85 to 99.58% nucleotide identity among themselves.

scholarly journals High-resolution mapping of Rym14Hb, a wild relative resistance gene to barley yellow mosaic disease

2020 ◽  
Author(s):  
Hélène Pidon ◽  
Neele Wendler ◽  
Antje Habekuβ ◽  
Anja Maasberg ◽  
Brigitte Ruge-Wehling ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Mechanism ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Linkage Drag ◽  
Resistance Locus ◽  
Wild Relative ◽  
Yellow Mosaic Disease ◽  
Barley Breeding

Abstract Key message We mapped the Rym14Hb resistance locus to barley yellow mosaic disease in a 2Mbp interval. The co-segregating markers will be instrumental for marker-assisted selection in barley breeding. Abstract Barley yellow mosaic disease is caused by Barley yellow mosaic virus and Barley mild mosaic virus and leads to severe yield losses in barley (Hordeum vulgare) in Central Europe and East-Asia. Several resistance loci are used in barley breeding. However, cases of resistance-breaking viral strains are known, raising concerns about the durability of those genes. Rym14Hb is a dominant major resistance gene on chromosome 6HS, originating from barley’s secondary genepool wild relative Hordeum bulbosum. As such, the resistance mechanism may represent a case of non-host resistance, which could enhance its durability. A susceptible barley variety and a resistant H. bulbosum introgression line were crossed to produce a large F2 mapping population (n = 7500), to compensate for a ten-fold reduction in recombination rate compared to intraspecific barley crosses. After high-throughput genotyping, the Rym14Hb locus was assigned to a 2Mbp telomeric interval on chromosome 6HS. The co-segregating markers developed in this study can be used for marker-assisted introgression of this locus into barley elite germplasm with a minimum of linkage drag.

scholarly journals Management of Yellow Mosaic Disease of Mungbean Against Mungbean Yellow Mosaic Virus (MYMV) in Bangladesh

2018 ◽  
Vol 1 (2) ◽  
pp. p100
Author(s):  
Md. S. Islam ◽  
Md. B. Hossain ◽  
Saleh A. Shahriar ◽  
Fatema Begum ◽  
Md. N. H. Sani
Keyword(s):  
Mosaic Virus ◽  
Disease Severity ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Economic Damage ◽  
Yellow Mosaic Disease ◽  
Yield Attributes ◽  
Mungbean Yellow Mosaic Virus

The prime aim of the study was to manage of Yellow mosaic disease of mungbean against Mungbean yellow mosaic virus (MYMV) by using one newly release botanical nutrient and through three selected insecticides. BARI (Bangladesh Agricultural Research Institute) released variety BARI mung-5, three insecticides (Imidacloprid, Acmix and Sobicron) and one botanical nutrient PPN (Peak performance nutrients) were used in the experiment. The plants were grown for pulse production and natural inoculums were relied upon for the infection of MYMV. Growth parameters, yield attributes and physiological features were significantly influenced by the application of selected insecticides and PPN combinations. Disease incidence and disease severity of MYMV were significantly varied among the treatments. Application of Imidacloprid with PPN combination gave the lowest disease incidence (3.13, 5.24 and 6.24% per plot and 14.33, 15.49 and 21.87% per plant) at 30, 40 and 50 DAS, respectively while the highest disease incidence (7.77, 13.70 and 19.24% per plot and 39.33, 48.20 and 56.63% per plant) were found in control at 30, 40 and 50 DAS, respectively. Application of Imidacloprid with PPN also gave the lowest disease severity (5.00, 6.00 and 13.33% at 30, 40 and 50 DAS, respectively while the highest disease severity (27.33, 35.00 and 45.00%) at 30, 40 and 50 DAS, respectively were measured in control treatment when no insecticides and PPN was used. If the disease is established once in the field then it is difficult to manage. As the disease is transmitted by vector (whitefly), the growers are suggested to control the vector populations before reaching economic damage and severe disease infection.

Deployment of yellow mosaic disease resistance in mungbean [Vigna radiata (L.) Wilczek] through interspecific hybridization

2019 ◽  
Author(s):  
Navkiran Kaur Ludhar ◽  
Asmita Sirari ◽  
T.S. Bains ◽  
Abhishek Sharma ◽  
S.A.H. Patel ◽  
...  
Keyword(s):  
Interspecific Cross ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
New Delhi ◽  
Yellow Mosaic Disease ◽  
Rice Bean ◽  
Kharif Season ◽  
Mungbean Yellow Mosaic Virus ◽  
Interspecific Derivatives ◽  
Favourable Environment

Mungbean yellow mosaic virus [Urdbean: New Delhi: 2011] Accession no. JQ398669.1, was found to be prevalent in both summer and kharif season causing Yellow Mosaic Disease (YMD) of mungbean. Since similar strain was observed in both the seasons, the short duration interspecific derivative lines of mungbean suitable for summer season were evaluated for YMD resistance in kharif season- a highly favourable environment for YMD epidemics during 2014-2016. Out of fourteen lines showing stable resistant reaction, few lines promising for other economically important traits were selected and again evaluated by whitefly mediated artificial inoculation technique for confirmation of resistance during 2017. The selected promising interspecific derivative lines showed no YMD symptoms except SML 1829. SML 1827 derived from interspecific cross between mungbean and rice bean showed highest yield among all interspecific derivatives. Further, rice bean specific segments were also identified in SML 1827 with SSR markers.

Status of mungbean yellow mosaic disease of french bean in different agro-climatic zones of Karnataka

2017 ◽  
Vol 54 (1) ◽  
pp. 141
Author(s):  
T C Archith ◽  
V Devappa ◽  
B Fakrudin ◽  
Amrutha S Bhut ◽  
Vishnuvardhana
Keyword(s):  
French Bean ◽  
Mosaic Disease ◽  
Yellow Mosaic Disease ◽  
Climatic Zones
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