Purification, Characterization and Bioefficacy of Legume Lectins against Mustard Aphid

2021 ◽  
Author(s):  
Deeksha . ◽  
Manjeet Kaur Sangha ◽  
Manju Bala ◽  
Sucheta Sharma
Keyword(s):  
Glycine Max ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Lens Culinaris ◽  
Carbohydrate Binding ◽  
Potential Candidate ◽  
Confidential Interval ◽  
Mustard Aphid ◽  
Lc50 Value ◽  
Lentil Lectin

Background: Lectins are carbohydrate binding proteins which perform diverse roles in plants. One important role is in plant defense. These proteins hold great potential as entomotoxic proteins as a part of integrated pest management. Methods: Lectins were purified and characterized from seeds of two legumes, Glycine max-Soybean and Lens culinaris-Lentil, employing ammonium sulfate fractionation and ion exchange chromatography. Bioefficacy of the purified lectins was evaluated against mustard aphid.Result: Lectins isolated from seeds of soybean (Glycine max agglutinin GMA-I, II) and lentil (Lens culinaris agglutinin LCA-I) were purified upto 9.30 (GMA-I), 4.60 (GMA-II) and 8.70 (LCA-I) fold, respectively. Lectin characterization revealed that soybean agglutinin and lentil agglutinin were specific towards D-Galactose and D-mannose, respectively. Insect bioassay was carried out with five different concentrations (10, 20, 30, 40, 50 µg/ml) of purified lectins of soybean and lentil against mustard aphid. The lethal concentration LC50 value for GMA-I was obtained as 32.1 µg/ml with a 95% confidential interval of 18.2 to 40.5 µg/ml and that of LCA-I was 19.1 µg/ml with a 95% confidential interval of 9.3 to 26.8 µg/ml. Lentil lectin (LCA-I) with lower LC50 value, was found to be the potential candidate for integrated pest management.

scholarly journals Effectiveness of Biocontrol Based IPM Modules Against Lipaphis Erysimi Kaltenbach (Hemiptera: Aphididae)

2008 ◽  
Vol 48 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Rakesh Pandey ◽  
Narendra Singh
Keyword(s):  
Integrated Pest Management ◽  
Pest Management ◽  
Environmental Values ◽  
Field Conditions ◽  
Aphid Population ◽  
Lipaphis Erysimi ◽  
Chrysoperla Carnea ◽  
Minimum Level ◽  
Mustard Aphid ◽  
Oilseed Crops

Effectiveness of Biocontrol Based IPM Modules Against Lipaphis Erysimi Kaltenbach (Hemiptera: Aphididae)Rapessed and mustard are important oilseed crops in India. The vulnerability of the crop to mustard aphid,Lipaphis erysimiKaltenbach is the main bottleneck in successful cultivation of this crop. In order to use insecticides at a minimum level, effectiveness of different Integrated Pest Management (IPM) modules were tested under field conditions. The module (NSKE +Chrysoperla carnea) proved most effective in reducing the aphid population in terms of socio-economic and environmental values.

Electron investigations of rhabdovirus-like particles in mexican bean beetles and crickets

1978 ◽  
Vol 36 (2) ◽  
pp. 378-379
Author(s):  
J. R. Adams ◽  
G. J Tompkins ◽  
A. M. Heimpel ◽  
E. Dougherty
Keyword(s):  
Biological Control ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Developmental Stages ◽  
Management Program ◽  
Acheta Domesticus ◽  
Epilachna Varivestis ◽  
House Cricket ◽  
Similar Morphology ◽  
Bean Beetle

As part of a continual search for potential pathogens of insects for use in biological control or on an integrated pest management program, two bacilliform virus-like particles (VLP) of similar morphology have been found in the Mexican bean beetle Epilachna varivestis Mulsant and the house cricket, Acheta domesticus (L. ).Tissues of diseased larvae and adults of E. varivestis and all developmental stages of A. domesticus were fixed according to procedures previously described. While the bean beetles displayed no external symptoms, the diseased crickets displayed a twitching and shaking of the metathoracic legs and a lowered rate of activity.Examinations of larvae and adult Mexican bean beetles collected in the field in 1976 and 1977 in Maryland and field collected specimens brought into the lab in the fall and reared through several generations revealed that specimens from each collection contained vesicles in the cytoplasm of the midgut filled with hundreds of these VLP's which were enveloped and measured approximately 16-25 nm x 55-110 nm, the shorter VLP's generally having the greater width (Fig. 1).

Prospects for Integrated Pest Management of Brown Marmorated Stink Bug in Washington Tree Fruits

2019 ◽  
Vol 30 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Elizabeth H. Beers ◽  
Adrian Marshall ◽  
Jim Hepler ◽  
Josh Milnes
Keyword(s):  
Integrated Pest Management ◽  
Pest Management ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Tree Fruits

Integrated Pest Management of Manifestations as Infestations: Angels are Insects

2004 ◽  
Vol 10 (3) ◽  
pp. 22-25
Author(s):  
Sally Y. Shelton ◽  
John E. Simmons ◽  
Tom J.K. Strang
Keyword(s):  
Integrated Pest Management ◽  
Pest Management

Development of REDCAHOR—a Vegetable Research and Development Network in Central America

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 531b-531
Author(s):  
J. Nienhuis
Keyword(s):  
Integrated Pest Management ◽  
Pest Management ◽  
Central America ◽  
Organic Production ◽  
Vegetable Production ◽  
Greenhouse Production ◽  
Short Course ◽  
Development Network ◽  
Breeding And Genetics ◽  
The Impact

REDCAHOR is the Spanish acronym for “Central American Vegetable Network.” Vegetables have traditionally been an important source of nutrients and vitamins in the diet in Central America. Vegetable production in this region is now changing as local consumers are demanding increased diversity and quality and international markets are expanding with “non-traditional” vegetable exports. The present restraints to expanded research and production of vegetables in the region include i) need for cultivars with increased insect and disease resistance, ii) poor and excessive use of pesticides, and iii) inadequate postharvest technology. In addition, there are few vegetable researchers in the region and response to their activities have not been coordinated. The goal of REDCAHOR is to develop a regional network of national institutions that can prioritize agendas and cooperate to maximize the impact of available resources. Establishment of a system of regional trials and cooperative regional programs in integrated pest management and plant breeding are currently under development. A series of regional workshops are planned, including integrated pest management, maintenance and use of genetic resources, organic production, and greenhouse production. In addition, REDCAHOR, in collaboration with the Escuela Agricola Panamerica in Honduras, will offer regional short-course training in vegetable breeding and genetics as well as vegetable production and management, including integrated pest management.

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