Single Marker Analysis in Groundnut for Tolerance to in vitro Seed Colonization by Aspergillus flavus and Aflatoxin Contamination

2020 ◽  
Author(s):  
Hasanali Nadaf ◽  
B.N. Harish Babu ◽  
G. Chandrashekhara ◽  
D.L. Savithramma ◽  
Manjunath K. Naik
Keyword(s):  
Ssr Markers ◽  
Aspergillus Flavus ◽  
Phenotypic Variation ◽  
Aflatoxin Contamination ◽  
Health Concern ◽  
Single Marker Analysis ◽  
Marker Analysis ◽  
Single Marker ◽  
Seed Colonization

Background: Aflatoxin contamination in groundnut is a serious health concern for both humans and ruminants. Genetic resistance is a viable, cost-effective and eco-friendly approach to manage aflatoxin contamination. Molecular markers particularly SSRs have been proved to be very effective in Marker Assisted Selection.Methods: In this study, single marker analysis using 30 SSR markers in 66 groundnut genotypes was executed to know if any selected SSRs were linked to in vitro seed colonization by Aspergillus flavus (IVSCAF) and/or aflatoxin contamination.Result: Single marker analysis revealed significant association of few SSR markers with tolerance to IVSCAF and/or aflatoxin contamination. Four markers viz., GM-1954, GM-1883, pPGPseq-2F05 and S-03 were found to be associated with in vitro seed colonization by A. flavus. The marker GM-1954 has shown a maximum R2 value of 14.07 indicating that 14.07 per cent phenotypic variation for IVSCAF has been explained by this marker (F=0.002**). Further, three markers viz., S-21, S-80 and GM-1954 were found to be associated with tolerance to aflatoxin contamination. It is evident from the results that, the marker GM 1954 has shown association with both IVSCAF as well as aflatoxin contamination. However, the R2 value of GM 1954, which explains the phenotypic variation for aflatoxin contamination, was less (6.21) as compared to that of IVSCAF (14.07).

scholarly journals Identification of Putative Molecular Markers Associated with Nut Weight and Kernel Weight and Four Growth Traits in Cashew

2020 ◽  
pp. 262-274
Author(s):  
E. Eradasappa ◽  
G. S. Mohana ◽  
J. D. Adiga ◽  
Siddanna Savadi
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Growth Traits ◽  
Tree Height ◽  
Kernel Weight ◽  
Growth And Yield ◽  
Phenotypic Variance ◽  
Single Marker Analysis ◽  
Marker Analysis ◽  
Single Marker

Aim: To identify putative molecular markers linked to some useful traits in cashew using SSR markers in the F1 population. Place and Duration of the Study: Study conducted at ICAR-Directorate of Cashew Research, Puttur, Karnataka, India during 2013-17. Methodology: Population for the study comprised of 83 F1 plants developed through crossing tall variety Ullal-3 as female parent with dwarf accession NRC-492 as male parent which was planted in the field at 6m x 6m spacing during the year 2009. It was phenotyped along with parents during the year 2017 for the 13 growth and yield related traits and genotyped using 32 polymorphic SSR markers from cashew, almond, pistachio and mango. Single marker analysis was deployed to identify the markers linked to traits by SPSS software. Results: results revealed three markers such as AL 29, IM 31 and IM 28 to be significantly associated with some traits. Marker AL 29 from Almond species was linked to traits stem girth, nut weight and kernel weight with an explained phenotypic variance of 7.9%, 5.6% and 5.4%, respectively. Marker IM 31 from mango species was associated with stem girth, kernel weight, tree height, mean tree spread, nut weight and presented phenotypic variance of 17.6%, 5.7%, 8.2%, 4.5, 4.2% in that order. While the marker IM 28 from mango species showed linked with stem girth and intermodal length with phenotypic variance of 7.6%, 7.5%, 10.4% correspondingly. Conclusion: The present study has identified three markers AL 29  (from Almond) , IM 28 and IM  31 (from Mango) linked to six traits viz., stem girth, tree height, mean tree spread, intermodal length, nut weight and kernel weight with the limited polymorphic markers using Single Marker Analysis. However, further studies are aimed to validate the linked markers prior to using them in marker assisted selection in cashew.

scholarly journals Resistance of Peanut Genotypes to Seed Infection by Aspergillus Flavus in Field Trials in India1

1987 ◽  
Vol 14 (1) ◽  
pp. 17-21 ◽  
Author(s):  
V. K. Mehan ◽  
D. McDonald ◽  
K. Rajagopalan
Keyword(s):  
Aspergillus Flavus ◽  
Macrophomina Phaseolina ◽  
Field Trials ◽  
Field Resistance ◽  
Aflatoxin Contamination ◽  
Seed Infection ◽  
Seed Colonization ◽  
Positive Correlations ◽  
Resistant Genotypes

Abstract Eleven peanut genotypes, six resistant and five susceptible to in vitro seed colonization by Aspergillus flavus Link (IVSCAF), were evaluated for field resistance to seed infection by A. flavus and other soil fungi, and for aflatoxin contamination, in seven environments in southern India. Five of the IVSCAF-resistant genotypes had significantly greater resistance to infection of seed by A. flavus in the field and had lower aflatoxin contamination than the IVSCAF-susceptible genotypes. Resistance to field infection of seed by A. flavus was stable across the seven environments. Significant interactions were found between environments and IVSCAF-susceptible genotypes for infection by A. flavus, Aspergillus niger van Tiegh, and Macrophomina phaseolina (Tassi.) Goid. Genotypes with field resistance to A. flavus also had significantly less seed infection by A. niger, M. phaseolina, and Fusarium spp. than had the A. flavus-susceptible genotypes. Significant positive correlations were found between IVSCAF-resistance and field resistance to A. flavus seed infection, and between the seed infection and aflatoxin B1 contamination. The field resistant genotypes J 11, Ah 7223, UF 71513, U 4–7–47 have yield levels and pod and seed characters acceptable in India.

scholarly journals Identifikasi Penanda SSR yang Berasosiasi dengan Bobot Tandan Buah Kelapa Sawit (Elaeis guineensis Jacq.)

2017 ◽  
Vol 45 (1) ◽  
pp. 79-85
Author(s):  
Dwi Yono ◽  
Yudiwanti Wahyu ◽  
Sobir , ◽  
Dan Nurita Toruan-Mathius
Keyword(s):  
Ssr Markers ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Allelic Diversity ◽  
Complex Trait ◽  
Molecular Data ◽  
Breeding Cycle ◽  
Single Marker Analysis ◽  
Marker Analysis ◽  
Single Marker

Oil palm is a perennial oil crop that contribute the most important source of vegetable oil in the world. Oil palm breeding cycle take a long period, therefore molecular marker-assisted selection (MAS) is required to shorten the selection time. This MAS require the associated marker to desired trait, particularly for yield and the component that as a complex trait and depend on genetic background. The aim of this study was to obtain SSR marker that associated with average of bunch weight (ABW). Plant material was used in this study is Tenera population derived from Deli Dura x AVROS Pisifera. Seven SSR markers from public database were associated to ABW trait. Molecular data analysed for SSR markers profile which consisted of allelic diversity, heterozygosity level, and polymorphism information content (PIC). Association between SSR markers to ABW traits was performed with single marker analysis using one way analysis of variance. The results showed that SSR markers were able to amplify DNA with two to three number of alleles with the average of 2.3 alleles per locus. ABW trait for this population was significantly associated with mEgCIR3428 marker. This marker may be used in the selection of Deli Dura and AVROS Pisifera palm on the next selection cycle.Keywords: Average of bunch weight, AVROS Pisifera, Deli Dura, single marker analysis

scholarly journals A Novel Niosome-Encapsulated Essential Oil Formulation to Prevent Aspergillus flavus Growth and Aflatoxin Contamination of Maize Grains During Storage

Toxins ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 646 ◽  
Author(s):  
García-Díaz ◽  
Patiño ◽  
Vázquez ◽  
Gil-Serna
Keyword(s):  
Aspergillus Flavus ◽  
Fungal Growth ◽  
Storage Conditions ◽  
Aflatoxin Contamination ◽  
Small Scale ◽  
Low Concentrations ◽  
Oil Formulation ◽  
Scale Test ◽  
Encapsulation Method

Aflatoxin (AF) contamination of maize is a major concern for food safety. The use of chemical fungicides is controversial, and it is necessary to develop new effective methods to control Aspergillus flavus growth and, therefore, to avoid the presence of AFs in grains. In this work, we tested in vitro the effect of six essential oils (EOs) extracted from aromatic plants. We selected those from Satureja montana and Origanum virens because they show high levels of antifungal and antitoxigenic activity at low concentrations against A. flavus. EOs are highly volatile compounds and we have developed a new niosome-based encapsulation method to extend their shelf life and activity. These new formulations have been successfully applied to reduce fungal growth and AF accumulation in maize grains in a small-scale test, as well as placing the maize into polypropylene woven bags to simulate common storage conditions. In this latter case, the antifungal properties lasted up to 75 days after the first application.

scholarly journals In Vitro Activity of Balanites aegyptiaca and Tamarindus indica Fruit Extracts on Growth and Aflatoxigenicity of Aspergillus flavus and A. parasiticus

2013 ◽  
Vol 2 (4) ◽  
pp. 68 ◽  
Author(s):  
Saifeldin Ahmed El-nagerabi ◽  
Abdulkadir E. Elshafie ◽  
Mohamed R. Elamin
Keyword(s):  
Aspergillus Flavus ◽  
Fungal Growth ◽  
Aflatoxin Contamination ◽  
Tamarindus Indica ◽  
Balanites Aegyptiaca ◽  
Total Aflatoxin ◽  
Animal Products ◽  
Fruit Extracts ◽  
Aflatoxin B

<p>Aflatoxin and especially aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) is a carcinogenic secondary metabolite synthesized by certain <em>Aspergillus </em>species. They contaminate natural and processed agricultural and animal products which render them unfit for consumption. The aim of this study was to evaluate the <em>in vitro</em> effects of <em>Balanites aegyptiaca</em> and <em>Tamarindus indica</em> fruit extracts on the growth and aflatoxin secretion of <em>Aspergillus flavus</em> (SQU21) and <em>A. parasiticus </em>(CBS921.7) strains. The two fruit extracts significantly (<em>P </em>&lt; 0.05) reduced aflatoxin and did not inhibit mycelial dry weights of the two <em>Aspergillus </em>strains. At different concentrations of balanites (2.5-10%), the inhibition of total aflatoxin was 49.9-84.8% for <em>A. flavus</em> (SQU21) and 32.1-84.4% for <em>A. parasiticus</em> (CBS921.7), whereas the inhibition of aflatoxin Bwas 38.2-81.4% and 32.8-80.6% for the two strains. Tamarind fruit extract (2.5-7.5%) caused 28.8-84.2% and 40.7-85.5% reductions in total aflatoxin and 37.1-83.5% and 33.9-85.9% in aflatoxin B for the two strains, respectively. None of these extracts inhibited the fungal growth or detoxified synthetic aflatoxin B<sub>1</sub>. We have concluded that these fruits contain various inhibitors to aflatoxin biosynthesis and secretion. Therefore, they can be used in combination as safe green biopreservatives to combat aflatoxin contamination of food.</p>

Potential SNPs related to microspore culture in Raphanus sativus based on a single-marker analysis

2018 ◽  
Vol 98 (5) ◽  
pp. 1072-1083 ◽  
Author(s):  
Yong Suk Chung ◽  
Yun Gyeong Lee ◽  
Renato Rodrigues Silva ◽  
Suhyoung Park ◽  
Min Young Park ◽  
...  
Keyword(s):  
Raphanus Sativus ◽  
Microspore Culture ◽  
P Value ◽  
Nucleotide Polymorphisms ◽  
Single Marker Analysis ◽  
Regeneration Rate ◽  
Marker Analysis ◽  
Wide Range ◽  
Single Marker ◽  
High Regeneration

Radish (Raphanus sativus) is an economically important crop grown for its edible roots and leaves. It is a self-incompatible, outcrossing species, making the production of homozygous lines and the development of breeding populations difficult. However, this can be overcome with haploids production techniques using isolated microspores, providing the rapid production of homozygous lines for breeding. Thus, it would be useful to identify radishes with a high regeneration rate from microspore culture. In the current study, 96 radish cultivars or germplasms were evaluated for high regeneration rates. Also, a single-marker analysis (SMA) was applied to detect single nucleotide polymorphisms (SNPs) potentially associated with this trait using genotype-by-sequencing (GBS) technology. The regeneration rate from microspore culture of 96 lines showed a wide range, from 0% to 269.5%. From the SMA, 52 markers were detected at a p value of 0.001 and a total of 11 physically nearby genes with high levels of similarity in various species were identified as candidates for high regeneration rates. This result could be used for clarifying the genetic basis underlying these traits and developing molecular markers associated with regeneration rates and would be beneficial for generating homozygous inbred lines.

scholarly journals Resistance to Aspergillus flavus in Corn Kernels Is Associated with a 14-kDa Protein

1998 ◽  
Vol 88 (4) ◽  
pp. 276-281 ◽  
Author(s):  
Z.-Y. Chen ◽  
R. L. Brown ◽  
A. R. Lax ◽  
B. Z. Guo ◽  
T. E. Cleveland ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Trypsin Inhibitor ◽  
Aflatoxin Contamination ◽  
Inhibitor Protein ◽  
Trypsin Activity ◽  
Terminal Sequence ◽  
High Concentration ◽  
Resistant Varieties ◽  
Corn Kernels

Corn genotypes resistant or susceptible to Aspergillus flavus were extracted for protein analysis using a pH 2.8 buffer. The profile of protein extracts revealed that a 14-kDa protein is present in relatively high concentration in kernels of seven resistant corn genotypes, but is absent or present only in low concentration in kernels of six susceptible ones. The N-terminal sequence of this 14-kDa protein showed 100% homology to a corn trypsin inhibitor. The 14-kDa protein purified from resistant varieties also demonstrated in vitro inhibition of both trypsin activity and the growth of A. flavus. This is the first demonstration of antifungal activity of a corn 14-kDa trypsin inhibitor protein. The expression of this protein among tested genotypes may be related to their difference in resistance to A. flavus infection and subsequent aflatoxin contamination.

scholarly journals Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Spurthi N Nayak ◽  
Gaurav Agarwal ◽  
Manish K Pandey ◽  
Hari K Sudini ◽  
Ashwin S Jayale ◽  
...  
Keyword(s):  
Immune Responses ◽  
Aspergillus Flavus ◽  
Host Pathogen ◽  
Seed Colonization
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