In Vitro Investigation of Biopriming on Seed Quality Parameters in Green Gram [Vigna radiata (L.)]

Author(s):  
Ashok S Sajjan ◽  
Sujaya Waddinakatti ◽  
R B Jolli ◽  
Geetha D Goudar

The in vitro investigation was conducted during the kharif 2017 in the Department of Seed Science and Technology, College of Agriculture, Vijayapura. The experiment comprised of nine biopriming treatments along with control. The experiment was laid out in CRD with three replications. The seeds were subjected to in vitro studies and data was analysed statistically. The seed bioprimed with Rhizobium leguminosarum @ 20 per cent recorded higher seed germination percentage (90.50 %), shoot length (19.27 cm), root length (12.38 cm), seedling length (31.65 cm), seedling dry weight (373.0 mg), seedling vigour index I (2864), seedling vigour index II (33.75), dehydrogenase enzyme activity (1.010 OD value) and lower electrical conductivity (0.107 dS m-1) followed by Pseudomonas fluorescens @ 20 per cent, Fluorescent pseudomonad sp. @ 20 per cent as compared to control.

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.


2017 ◽  
Vol 9 (4) ◽  
pp. 2338-2343
Author(s):  
Arpana D. Vaja ◽  
J. B. Patel ◽  
R.N. Daki ◽  
Shital A. Chauhan

The present investigation on effect of nitrogen [N0 (Control), N1 (50 kg N/ha), N2 (100 kg N/ha), and N3 (150 kg N/ ha)] and plant growth regulators [G0 (Control), G1 (50 ppm GA3), G2 (50 ppm NAA) and G3 (500 ppm Cycocel)] on seed yield per plant and seed quality parameters in brinjal (Solanum melongena L.) cv. GJB 3 was carried out at the Instructional Farm, Department of Agronomy, Junagadh Agricultural University, Junagadh during kharif 2015-16. The experiment was laid out in field as per randomized block design (Factorial) with three replications. The seed harvested from 16 different treatments combinations replicated thrice from the field were analyzed in the laboratory following completely randomized design (factorial) for various seed quality parameters. Application of nitrogen @ 150 kg N/ha recorded significantly (P<0.05) highest seed yield per plant, shoot fresh weight, root dry weight, shoot dry weight and vigour index – 2 (mass), while application of 100 kg N/ha resulted in significantly (P<0.05) highest germination percentage, root length, shoot length, root fresh weight and vigour index – 1 (length). Application of GA3 at 50 ppm recorded significantly the highest seed yield per plant, germination percentage, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, vigour index – 1 (length) and vigour index – 2 (mass). Among the 16 different treatment combinations, nitrogen @ 150 kg/ha and GA3 50 ppm noted the maximum seed yield per plant, shoot dry weight and vigour index – 2 (mass), while significantly the maxi-mum germination percentage, root length, shoot length, root fresh weight, shoot fresh weight and vigour index – 1 (length) were registered in treatment combination nitrogen 100 kg N/ha and GA3 @ 50 ppm. Therefore, it is advised that application of 100-150 kg of N/ha as a nitrogenous fertilizer and spray GA3 @ 50 ppm (G1) at 45 days after transplanting helps in increasing the seed yield per plant and seed quality parameters in brinjal seed production.


2016 ◽  
Vol 8 (1) ◽  
pp. 340-345
Author(s):  
N. Amruta ◽  
P. J. Devaraju ◽  
Mangalagowri Mangalagowri ◽  
S. P. Kiran ◽  
H. P. Ranjitha ◽  
...  

A field experiment was conducted to assess the response of nutrient levels and spacing on seed quality attributes of black gram cv. LBG-625 (Rashmi). Experimental results revealed that fertilizer application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- Bacillus megaterium (250 g ha-1) recorded highest test weight (39.27 g), germination (90.60%), root length (15.77 cm), shoot length (13.43 cm), mean seedling length (29.20 cm), mean seedling dry weight (57.99 mg), seedling vigour index-I (2656), seedling vigour index-II (525), total dehydrogenase activity (0.998), protein content (23.16%), field emergence (86.56 %) lowest electrical conductivity (0.813 dSm-1) were superior over other fertilizer treatments. Among the planting geometry 60 x 10 cm recorded more germination per cent (90.48 %) compared to 45 x 10 cm (87.64 %) and 30 x 10 cm (86.91 %). Interaction of nutrient levels and spacing through the application of 50:100:100 + black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm recorded more mean seedling length (34.40 cm), mean seedling dry weight (58.30 mg),and field emergence (90.24 %) lowest electrical conductivity (0.776 dSm-1) compared to control. The application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm were considered as seed quality improvement approach in blackgram, therefore conjunctive use of inorganic fertilizers and biofertilizer may be suggested for higher seed quality parameters along with overall betterment of crop.


2017 ◽  
Vol 51 (05) ◽  
Author(s):  
P.R. Arvind Kumar ◽  
B. C. Channakeshava ◽  
R. Siddaraju

An experiment was carried to find out the effect of sowing dates and cutting time on seed yield and quality of alfalfa cv. RL-88. The results of the experiment revealed that, the significantly higher seed yield per plant (1.491 g), seed yield per plot (161.07 g) and seed yield (355.00 kg ha-1) was noticed in 15th August sowing and the cutting carried out at 60 days after sowing. Whereas, the seed quality parameters of the resultant seeds viz., highest seed germination (95.00 %), seedling length (13.90 cm), seedling dry weight (412.69 mg) and seedling vigour index (1320) found highest in15th August sowing and the cutting followed at 60 days after sowing.


Author(s):  
C. H. Sai Kiran ◽  
Arun Kumar Chaurasia ◽  
Pradeep Kumar Shukla ◽  
Krishna Sai Thammineni ◽  
P. V. Raghunath Reddy

Quinoa is a saline-tolerant crop and highly nutritious compared with rice, wheat, and oats. Quinoa cultivar EC507704 were used to check the performance of seed invigoration with organic treatments under different salinity levels current experiment was conducted in 2021 at the seed testing laboratory department of Genetics and Plant Breeding, Naini Agriculture Institute, Prayagraj (U. P.). Experimentation was carried out by using Complete Randomized Design with four replications by using top of the paper method. The salinity levels were obtained by dissolving 0mM, 100mM, and 200mM NaCl in one liter of distilled water. Filter papers were supplied with a salt solution to place the treated seeds. The treatments used in this experiment are T0 distilled water as Control, T1 and T2 Seaweed 5% and 10%, T3,T4,T5 Panchagavya 4%,6% and 10%, T6,T7,T8 and T9 Beejamrutha 25%,50%,75% and 100%. The results indicated the superiority with the interaction of treatment and salinity of T9S0 Beejamrutha 100% in 0mM NaCl shows 92% in germination percentage, 3.60cm in shoot length, 6.85cm in root length, 10.45cm in seedling length, 0.25g in dry weight, 961.32 in Seedling vigour index compared with control. The better performance of Beejamrutha increases the seed quality parameters due to the presence of beneficial microorganisms, nutrients, and growth-inducing hormones.


Author(s):  
Pramod Sharma ◽  
Arun Bhatt ◽  
Rajesh Kanwar

The present investigation was carried out in the Department of Seed Science and Technology, College of Forestry, Ranichauri, Tehri Garhwal, Uttarakhand during 2015. A laboratory experiment was conducted to find out the influence of bio-priming on seed germination and seedling vigour. Seed germination was reported maximum for Psf-173 (93.66%) while minimum was reported for control (65.66%). Seed vigour parameter viz. root length (11.01 cm), shoot length (7.96 cm), seedling length (18.98 cm), fresh weight (8.00 g) and dry weight (1.31 g), vigour index I (1666.80) and vigour index II (115.56) was found higher for PSB treatment. Shilajeet variety had recorded significant higher values for most of the germination and vigour parameters. Shilajeet bio-primed with PSB attained higher values for majority of the quality parameters. Under laboratory condition results suggested that seed germination and seed vigour were greatly affected by different bio-agents. Among all the bio-agents, PSB showed positive influence on most of the quality parameter. However, other bio-agents had also showed significant effect over control.


2016 ◽  
Vol 8 (3) ◽  
pp. 1623-1628
Author(s):  
Pavan Shinde ◽  
S. R. Doddagoudar ◽  
S. N. Vasudevan

A laboratory experiment was carried out in the Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur to study the effect of seed polymer coating with micronutrients and foliar spray on the resultant seed quality parameters of chickpea. Among the seventeen different treatments, resultant chickpea seeds obtained from treatment polymer coated seeds (each@ 6 ml/kg of seed) along with the combination of micronutrients viz.,ZnSO4 + Boron + Ammonium molybdate + FeSO4 ( each @ 2 g/kg of seed ) and two foliar sprays (0.5 % + 0.2 % + 0.1% + 0.5 %, respectively, except ZnSO4 and FeSO4 in EDTA form) at an interval of 10 days during flowering stage (50 and 60 days after sowing) recorded significantly highest hundred seed weight (25.9 g), germination (97.00 %), speed of germination (18.50), shoot length (8.43 cm), root length (19.80 cm), seedling dry weight (43.30 mg) and seedling vigour index (2738) compared to all other treatments and control (23.6 g, 92.05 %, 13.74, 5.48 cm, 16.30 cm, 34.00 mg and 2004, respectively) and hence above treatment combinations can be used in order to produce good quality seeds.


Author(s):  
J. B. Maruthi ◽  
R. Paramesh

A laboratory experiment was conducted at the Department of Seed Science and Technology, GKVK, University of Agricultural Sciences, Bangalore. The results revealed that, application of recommended dose of NPK (30:80: 37.5 kg ha-1) + Recommended dose of FYM (10 t ha-1) +Brady rhizobium (250 g ha-1)+PSB (250 g ha-1) significantly improved the seed quality parameters viz., 100 seed weight (35.51g), seed size (0.76 cm<sup>3</sup>), seed density (1.55 g/cc), total dehydrogenase activity (4.80), germination (92.00 %), seedling length (30.69 cm), seedling dry weight (90.75 mg), seedling vigour index- I and II (2820 and 836 respectively), protein content (36.30 %) and field emergence (89.33 %) with lowest electrical conductivity (0.49 mScm-1) as compared to control and other treatments. Further, artificially aged seeds through accelerated ageing method also recorded higher seed quality parameters in the same treatment at the end of six days of ageing period.


Author(s):  
Shivasharanappa . ◽  
S. Patil ◽  
S. R. Doddagoudar ◽  
Vijay Kumar Kurnalliker ◽  
Rakesh . ◽  
...  

An attempt was made to predict the relative storability of seeds of soybean varieties (JS-335 and DSB-21) through accelerated ageing technique in comparison with that of natural ageing where both the varieties had similar initial germination (93 %). The seeds were subjected to accelerated ageing conditions at 41 oC temperature and 95 ± 5 per cent relative humidity up to 180 hours. While, for natural ageing, the seeds were packed in cloth bag with the initial moisture of 9.5 per cent and stored under ambient conditions. The experimental results revealed that with the subsequent increase in accelerated ageing duration from 24 hours onwards up to 180 hours, the seed quality parameters viz., seed germination percentage, mean germination time, seedling vigour index and dehydrogenase enzyme activity decreased significantly. While the electrical conductivity increased significantly. When the accelerated ageing results were compared with that of natural ageing, accelerated ageing for 132 hours (67 % germination) was nearly equal to nine months of natural ageing (69 % germination) in case of JS-335 whereas in DSB-21, 144 hours of accelerated ageing (70 % germination) was equal to nine months of natural ageing (70 % germination). Hence, the accelerated ageing test can be used to predict the relative storability of soybean seeds. Hence, the seed producers can take decision regarding which seed lot can be stored for longer time and which one should be immediately sold to the market without any compromise in seed quality. It also helps in taking other management decisions.


Author(s):  
M. , C. Muthu ◽  
Amlan Sushree ◽  
Rajkamal Srivastava

A field and laboratory experiment was conducted at Department of Seed Science and Technology, GKVK, UAS, Bangalore during 2014 to assess the response of fertilizer levels and spacing on seed quality parameters of green gram (Vigna radiata) cv. KKM-3. Experimental results revealed that fertilizer application of 50 :100 :100 NPK kg ha-1 + Green gram rhizobium (200gm) + PSB-Bacillus megaterium (200gm) recorded highest seed density (1.69g/cc), Test weight (46.2g), EC (0.750 dsm-1) TDH activity (0.999), germination percent age (97.25%), shoot length (21.98cm), rootlength (18.61cm), seedling dry weight (252g), seedlingvigour index I and II (3947 and 2450), protein percent (23.8%) and fieldemergence (90%) superior over other fertilizer treatments.Among the planting geometry 60×10cm recorded highest germination percentage (96.12%) as compared to 45×10cm (94.77%) and 30×10cm (93.82%). Interaction of fertilizerlevels and spacingthrough the application of 50 :100 :100 NPK kg ha-1 + green gram rhizobium (200gm) + PSB-Bacillus megaterium (200gm) withplantinggeometry 60×10cm recorded higher seed quality parameters viz Test weight (48.3 g), protein per cent (24.5 %), germination percentage (98%), EC (0.73dsm-1), TDH activity (1.001), seedling dry weight (265 mg), shoot length (23.65 cm), root length (19.36 cm) and seedling vigour index I and II (4224 and 2603) and field emergence( 93%) were observed.


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