Survey, collection and seed morphometric characterization of French bean (Phaseolus vulgaris L.) landraces of Himachal Pradesh

2017 ◽  
Author(s):  
Rajesh Kanwar ◽  
D. K. Mehta
Keyword(s):  
Hot Spot ◽  
Genetic Material ◽  
Coat Colour ◽  
French Bean ◽  
Himachal Pradesh ◽  
Seed Shape ◽  
Geographical Regions ◽  
Food And Nutrition ◽  
Cotyledon Colour ◽  
Breeding Programmes

The study was conducted to survey and collect various French bean genotypes from the main hot spot regions of Himachal Pradesh like Shimla, Chamba, Sirmour, Kullu, Kinnaur and some areas of Mandi and Solan district. Different bean genotypes were collected in a 15-day exploration trip from various geographical regions. The collected seed samples were evaluated and characterized for intra-specific seed morpho-metric characteristics like seed coat colour, hilum colour, seed shape, hypocotyls pigmentation, cotyledon colour pubescence on hypocotyls, 100 seed weight (g), seed length (mm) and seed width (mm). The evaluated genetic material was then deposited in the short term seed bank of Department of Seed Science and Technology UHF Nauni for future correspondence. The genetic diversity found among the landraces is of great importance in the utilization of the species for food and nutrition and more importantly for genetic improvement. The study would prove a basic primary step in germplasm recognisition activity for future breeding programmes and will project the local available genetic footprints to entire agro associated enterprises for necessary consideration.

Polymorphism of the СоІІ gene of honey bees in the western regions of Ukraine

2020 ◽  
Vol 12 (2) ◽  
pp. 174-179
Author(s):  
Oleksandr Cherevatov ◽  
Nadiia Roshka
Keyword(s):  
Molecular Markers ◽  
Apis Mellifera ◽  
Cytochrome Oxidase ◽  
Honey Bees ◽  
Molecular Taxonomy ◽  
Genetic Material ◽  
Gene Encoding ◽  
Geographical Regions ◽  
Coii Gene ◽  
Mitochondrial Cytochrome Oxidase

Due to active human intervention in natural genetic and population processes, survival and distribution areas of honey bees have been negatively affected. Preservation of the gene pool of aboriginal bees that are well adapted to local environmental conditions is an urgent problem that cannot be solved without the use of molecular methods to monitor the genetic composition of local populations. This type of research requires the use of state-of-the-art approaches based on application of molecular markers. In the molecular taxonomy of insects, mitochondrial cytochrome oxidase genes are widely used to identify closely related forms. The peculiarity of mtDNA is that it is maternally inherited, and the mitochondrial molecular markers are not separated by recombination. The СоІІ gene encoding the second subunit of cytochrome oxidase is widely used to descriminate the subspecies of honey bee. Therefore, to assess the distribution of different subspecies / breeds of Apis mellifera in Ukraine, the 5′-region of the СоІІ gene was sequenced and compared for bees from different geographical regions. In the CoII gene, base substitutions were found, which makes it possible to distinguish between honey bees of the Carpathian and Ukrainian steppe breeds, which are widespread in Ukraine. It has been revealed that the distribution of these breeds does not always correspond to the official zoning. The widespread practice in Ukraine of transporting the genetic material of Apis mellifera from different regions leads to uncontrolled hybridization and poses a threat to the preservation of aboriginal breeds of honey bees.

scholarly journals Evaluation of Red Raspberry Cultivars Used for Breeding and Commercial Growing in the Baltic Region

2017 ◽  
Vol 71 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Gunārs Lācis ◽  
Irita Kota-Dombrovska ◽  
Sarmīte Strautiņa
Keyword(s):  
Genetic Resources ◽  
Genetic Material ◽  
Climatic Conditions ◽  
Parent Material ◽  
Local Climate ◽  
Political Situation ◽  
Baltic Countries ◽  
Breeding Programmes ◽  
The Baltic ◽  
Local Advanced

Abstract The structure of raspberry cultivars and genetic resources in the Baltic countries have been influenced by the historical political situation in the 20th century and climatic conditions, especially winterhardiness. The genetic resources consist of some old European and American cultivars, but mostly of cultivars and hybrids bred in Russia. Currently, targeted breeding programmes are active only in Estonia and Latvia, which aim to develop winterhardy, disease-resistant cultivars, well adapted to the local climate. Therefore, parent material for hybridisation has been chosen from local advanced hybrids and introduced cultivars suitable to the regional climatic conditions. The aim of the study was to estimate the level of genetic diversity of Rubus germplasm and assess inter-specific and intra-specific relationships using phenotypical characterisation and molecular markers. Forty one Rubus genotypes were evaluated by 41 phenotypical traits and 15 previously described SSR markers. Both characterisation approaches discovered high correspondence with pedigree and a low level of diversity. A limited amount of the diversity of raspberry genetic material has been used in various breeding programmes, despite their broad geographical origin. The obtained results indicate the need for including local wild R. idaeus plant material into breeding programmes.

Oocytes from gilts of domestic pig (Sus scrofa domesticus) and wild boar (Sus scrofa) species exhibit similar developmental potential during in vitro maturation

1998 ◽  
Vol 1998 ◽  
pp. 178-178
Author(s):  
N.S. Prathalingam ◽  
K. Rust ◽  
M.E. Staines ◽  
G.J. McCallum ◽  
S.A. Edwards ◽  
...  
Keyword(s):  
Wild Boar ◽  
Sus Scrofa ◽  
In Vitro Maturation ◽  
Animal Species ◽  
Genetic Material ◽  
Developmental Potential ◽  
In Captivity ◽  
Breeding Programmes ◽  
Sus Scrofa Domesticus

In vitro embryo production strategies have been considered as possible means to protect wild and endangered animal species through assisted breeding programmes. They also offer the possibility to preserve genetic material from such stock or to facilitate breeding in captivity. The relevant technologies, however, have been developed to meet the needs of oocytes and embryos of domesticated animals and their suitability for wild species remains largely unknown. This study investigated the ability of in vitro maturation procedures, designed for oocytes of domestic pigs (Sus scrofa domesticus), to support the development of oocytes from wild boar (Sus scrofa)gilts.

scholarly journals Effect of biofertilizers on horticultural and yield traits in french bean var. Contender under dry temperate conditions of Kinnaur district of Himachal Pradesh

2018 ◽  
Vol 10 (1) ◽  
pp. 421-424
Author(s):  
Seema Thakur ◽  
Rajesh Thakur ◽  
Devinder Kumar Mehta
Keyword(s):  
Organic Matter ◽  
Block Design ◽  
French Bean ◽  
Himachal Pradesh ◽  
Research Station ◽  
Yield Traits ◽  
Calcium Ammonium Nitrate ◽  
Single Super Phosphate ◽  
Pod Length ◽  
Quality Production

Kinnaur district is known as the dry temperate zone of Himachal Pradesh and is known for off season and quality production of vegetables.In this district of Himachal Pradesh, Natural farming is mostly done with the minimum use of chemical fertilizers. Farmers are unaware of the judicious use of farm yard manure, and biofertilizers due to which yield of the french bean is very low (50-70 q/ha). French bean is one of the most important vegetables intercropped with apple in Kinnaur District. An experiment was conducted during the summer season of 2011 at the Experimental Farm of Vegetable Research Station, Kalpa, Kinnaur, Himachal Pradesh to study the effect of Rhizobium and Phosphorus Solublizing Bacteria (PSB) on the horticultural and yield traits in french bean var. Contender. Six treatments comprising seed treatments (with and without Rhizobium), seed treatment (with and without PSB) along with the combination of 60 % dose of recommended quantity of Calcium Ammonium Nitrate and 75 % dose of recommended quantity of Single Super Phosphate and organic matter were evaluated in a Randomized Complete Block Design (RCBD) with three replications. The results revealed that T5 treatment, i.e. Rhizobium+ PSB+ Organic matter resulted in more number of pods per plant (20), pod length (18 cm) and pod yield/ha (140 q/ha) .

Oocytes from gilts of domestic pig (Sus scrofa domesticus) and wild boar (Sus scrofa) species exhibit similar developmental potential during in vitro maturation

1998 ◽  
Vol 1998 ◽  
pp. 178-178
Author(s):  
N.S. Prathalingam ◽  
K. Rust ◽  
M.E. Staines ◽  
G.J. McCallum ◽  
S.A. Edwards ◽  
...  
Keyword(s):  
Wild Boar ◽  
Sus Scrofa ◽  
In Vitro Maturation ◽  
Animal Species ◽  
Genetic Material ◽  
Developmental Potential ◽  
In Captivity ◽  
Breeding Programmes ◽  
Sus Scrofa Domesticus

In vitro embryo production strategies have been considered as possible means to protect wild and endangered animal species through assisted breeding programmes. They also offer the possibility to preserve genetic material from such stock or to facilitate breeding in captivity. The relevant technologies, however, have been developed to meet the needs of oocytes and embryos of domesticated animals and their suitability for wild species remains largely unknown. This study investigated the ability of in vitro maturation procedures, designed for oocytes of domestic pigs (Sus scrofa domesticus), to support the development of oocytes from wild boar (Sus scrofa)gilts.

scholarly journals Identification and molecular characterization of Bean yellow mosaic virus infecting French bean in Himachal Pradesh

VirusDisease ◽  
2015 ◽  
Vol 26 (4) ◽  
pp. 315-318 ◽  
Author(s):  
P. N. Sharma ◽  
Vivek Sharma ◽  
Anuradha Sharma ◽  
Kajal Rajput ◽  
S. K. Sharma
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
French Bean ◽  
Himachal Pradesh ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

scholarly journals Mouse zygotes as recipients in embryo cloning

Reproduction ◽  
2006 ◽  
Vol 132 (5) ◽  
pp. 741-748 ◽  
Author(s):  
Pawel Gręda ◽  
Jolanta Karasiewicz ◽  
Jacek A Modliński
Keyword(s):  
Nuclear Membrane ◽  
Genetic Material ◽  
Coat Colour ◽  
New Technique ◽  
Glucose Phosphate Isomerase ◽  
Cell Stage ◽  
Glucose Phosphate ◽  
Mouse Zygotes ◽  
A New Technique ◽  
Embryo Cloning

Zygotes have not been recognized as nuclear recipients since enucleated zygotes receiving nuclei from beyond two-cell stage embryos are not able to form blastocysts. In the present study, a new technique of zygote enucleation is presented, which consists in selectively removing the nuclear membrane with genetic material of pronuclei, but leaving other pronuclear components in the cytoplasm. With selective enucleation it is possible – after transfer of eight-cell stage nuclei – to obtain 70.5 and 7.8% of preimplantation and full-term development respectively. Origin of cloned mice from introduced nuclei was confirmed by the coat colour and glucose phosphate isomerase (GPI) isozyme of the donor. We suggest that some pronuclear factors – taken away from the zygotes in the karyoplasts upon classical enucleation – are needed to reprogram the introduced nuclei.

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