Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek

2018 ◽  
Author(s):  
Prince Lekhi ◽  
R. K. Gill ◽  
Satinder Kaur ◽  
T. S. Bains
Keyword(s):  
Microsatellite Markers ◽  
Mosaic Virus ◽  
Vigna Radiata ◽  
Female Parent ◽  
Germination Percentage ◽  
Yellow Mosaic Virus ◽  
F1 Hybrids ◽  
Polymorphic Markers ◽  
Vigna Species ◽  
Mungbean Yellow Mosaic Virus

Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.

scholarly journals Breeding for Enhancing Legumovirus Resistance in Mungbean: Current Understanding and Future Directions

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 622 ◽  
Author(s):  
Chandra Mohan Singh ◽  
Poornima Singh ◽  
Aditya Pratap ◽  
Rakesh Pandey ◽  
Shalini Purwar ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Seed Quality ◽  
Soil Health ◽  
Mosaic Disease ◽  
Bipartite Begomoviruses ◽  
Yellow Mosaic Virus ◽  
Symbiotic Association ◽  
Detection Techniques ◽  
Vigna Species ◽  
Mungbean Yellow Mosaic Virus

Yellow mosaic disease (YMD) affects several types of leguminous crops, including the Vigna species, which comprises a number of commercially important pulse crops. YMD is characterized by the formation of a bright yellow mosaic pattern on the leaves; in severe forms, this pattern can also be seen on stems and pods. This disease leads to tremendous yield losses, even up to 100%, in addition to deterioration in seed quality. Symptoms of this disease are similar among affected plants; YMD is not limited to mungbean (Vigna radiata L. Wilczek) and also affects other collateral and alternate hosts. In the last decade, rapid advancements in molecular detection techniques have been made, leading to an improved understanding of YMD-causing viruses. Three distinct bipartite begomoviruses, namely, Mungbean Yellow Mosaic India Virus (MYMIV), Mungbean Yellow Mosaic Virus (MYMV), and Horsegram Yellow Mosaic Virus (HgYMV), are known to cause YMD in Vigna spp. Vigna crops serve as an excellent protein source for vegetarians worldwide; moreover, they aid in improving soil health by fixing atmospheric nitrogen through a symbiotic association with Rhizobium bacteria. The loss in the yield of these short-duration crops due to YMD, thus, needs to be checked. This review highlights the discoveries that have been made regarding various aspects of YMD affecting mungbean, including the determination of YMD-causing viruses and strategies used to develop high-yielding YMD-resistant mungbean varieties that harness the potential of related Vigna species through the use of different omics approaches.

Detection of QTLs associated with mungbean yellow mosaic virus (MYMV) resistance using the interspecific cross of Vigna radiata × Vigna umbellata

2019 ◽  
Vol 60 (3-4) ◽  
pp. 255-268 ◽  
Author(s):  
Mayalagu Kanimoli Mathivathana ◽  
Jayakodi Murukarthick ◽  
Adhimoolam Karthikeyan ◽  
Woojong Jang ◽  
Manickam Dhasarathan ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Vigna Radiata ◽  
Interspecific Cross ◽  
Yellow Mosaic Virus ◽  
Vigna Umbellata ◽  
Mungbean Yellow Mosaic Virus

Genetic analysis of gene‐specific resistance to mungbean yellow mosaic virus in mungbean ( Vigna radiata )

2019 ◽  
Vol 138 (2) ◽  
pp. 202-206 ◽  
Author(s):  
Akhouri Nishant Bhanu ◽  
Mahendra Narain Singh ◽  
Kartikeya Srivastava
Keyword(s):  
Genetic Analysis ◽  
Mosaic Virus ◽  
Vigna Radiata ◽  
Specific Resistance ◽  
Yellow Mosaic Virus ◽  
Mungbean Yellow Mosaic Virus

Understanding the inheritance of mungbean yellow mosaic virus (MYMV) resistance in mungbean (Vigna radiata L. Wilczek)

2017 ◽  
Vol 37 (5) ◽  
Author(s):  
Cayalvizhi B. Sai ◽  
Nagarajan P ◽  
Raveendran M ◽  
Rabindran R ◽  
Kannan Bapu J. R ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Vigna Radiata ◽  
Yellow Mosaic Virus ◽  
Mungbean Yellow Mosaic Virus

Search for Vigna species conferring resistance to Mungbean yellow mosaic virus in mungbean

2014 ◽  
Vol 13 (2) ◽  
pp. 162-167 ◽  
Author(s):  
M. Sudha ◽  
A. Karthikeyan ◽  
V. G. Shobhana ◽  
P. Nagarajan ◽  
M. Raveendran ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Pcr Analysis ◽  
Quantitative Detection ◽  
Interspecific Crosses ◽  
Rt Pcr ◽  
Vigna Species ◽  
Resistant Varieties ◽  
Mungbean Yellow Mosaic Virus ◽  
Polymerase Chain

Mungbean yellow mosaic virus (MYMV) is a disastrous pathogen of mungbean. It is widespread in most of southern India and no complete resistance has been identified among its commercial cultivars. Two isolates of MYMV, representing its diversity, were used to assess and characterize the susceptibility reaction of all the three species of Vigna. The seeds were agroinoculated with the virus and the presence of the viral DNA was confirmed after 12 d by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis after which the plants were monitored for the expression of symptoms. All of the 20 accessions of Vigna radiata and ten accessions of Vigna mungo were systemically infected with MYMV, and they all produced typical symptoms. On the other hand, the 24 accessions of Vigna umbellata were found to be resistant to both the isolates. For additional affirmation, three representative accessions of V. radiata and V. mungo and all the accessions of V. umbellata were agroinoculated, and quantitative RT-PCR was performed for the quantitative detection of the MYMV. The mRNA transcripts of MYMV were detected in V. radiata and V. mungo plants but not in the V. umbellata plants. Researching the molecular basis of the resistance in V. umbellate against MYMV might definitely be very constructive for developing resistant varieties of mungbean on a commercial scale. This genetic quality offering resistance to MYMV could also be incorporated into V. radiata/V. mungo by means of interspecific crosses.

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