Induced mutations in soybean (Glycine max L.)

Author(s):  
G. C. Shekar ◽  
Pushpendra .

Frequency of chlorophyll and viable mutations have been studied following seed treatment of soybean cv.PK 1029 with gamma rays, EMS and their combinations at varying doses. Mutation rate indicated that EMS @ 0.10 % was most potent in inducing the chlorophyll mutations whereas 20 kR was most efficient in inducing the viable mutations. The frequency and spectrum of the induced chlorophyll mutations was in the order of xantha, albino, chlorina and viridis. The frequency and spectrum of the induced viable mutations was in the order of plant type, leaf type, seed type and pod type. Combination of gamma rays and EMS treatment showed high lethality in inducing both chlorophyll and viable mutations.

Agriculture ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 248 ◽  
Author(s):  
Zhen Wang ◽  
Masoume Amirkhani ◽  
Suemar A.G. Avelar ◽  
Daibin Yang ◽  
Alan G. Taylor

Systemic seed treatment uptake was investigated in seeds and seedlings using fluorescent tracers to mimic systemic agrochemicals. Soybean was used as the model as soybean has the permeable seed coat characteristic to both charged and noncharged molecules. The purpose of the paper is to (1) screen 32 fluorescent tracers and then use optimal tracers for seed and seedling uptake, (2) investigate varietal differences in seed uptake, (3) examine the distribution of tracer uptake into 14-day-old seedlings, and (4) study the relationship between seed treatment lipophilicity, measured as log P on seed and root uptake. The major chemical families that displayed both seed and seedling uptake were coumarins and xanthenes. Seed uptake of coumarin 120 ranged from 1.1% to 4.8% of the applied seed treatment tracer from 15 yellow-seeded varieties. Rhodamine B, a xanthene compound uptake in seedlings, showed translocation from the applied seed treatment to all seedling tissues. Most of the tracer was measured in the hypocotyl and root, with lesser amounts in the epicotyl and true leaves. Log P is well documented in the literature to model systemic uptake by roots, but log P of the tracers were not related to seed uptake.


2021 ◽  
Vol 7 (2) ◽  
pp. 18674-18679
Author(s):  
Pablo Frezato ◽  
Artur Alves de Oliveira Braga ◽  
Maria Aparecida da Fonseca Sorace ◽  
Conceição Aparecida Cossa ◽  
Carlos Eduardo Monteiro Pires ◽  
...  

Agrikultura ◽  
2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Endah Yulia ◽  
Tarkus Suganda ◽  
Fitri Widiantini ◽  
Rangga Irawan Prasetyo

ABSTRACTAntifungal Effect of Aqueous Extract of Galangal (Alpinia galanga [L] Willd.) Rhizomeas Seed Treatment to Control Colletotrichum spp. of Soybean (Glycine max L.)Colletotrichum is one of the most important seed-borne pathogens of soybean which is usuallycontrolled with synthetic fungicide seed treatment. However, it is believed that the use ofsynthetic fungicide can cause a variety of negative impacts to the environment and humanhealth. Galangal rhizome extract has been widely reported to have antifungal and antibacterialproperties. The aim of the study was to investigate the effectiveness of galangal rhizomeaqueous extract as antifungal for pre-planting seed treatment to control Colletotrichum spp. insoybean. Laboratory and glasshouse experiments were carried out at the Department of PlantPests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The method used was anexperimental method to test the effectiveness of galangal rhizome aqueous extract against theemergence and spore germination suppression of Colletotrichum spp., and to test the seedviability and plant growth of soybean. Galangal rhizome extract with concentrations of 10%,30%, 50%, 70%, 90% and 100% as well as a metalaxyl fungicide (0.5 g/l) were applied as aseed treatment. The results showed galangal rhizome aqueous extract at concentration of100% reduced the presence of Colletotrichum spp. in seeds up to 100% after the treatment andsuppressed the spore germination by 76.20% as well as increased the seed viability and thegrowth of soybean plants.Keywords: Alpinia galanga, aqueous extract, seed treatment, soybean, Colletotrichum spp.ABSTRAKUmumnya pengendalian penyakit tular benih pada kedelai dilakukan melalui perlakuan benihdengan menggunakan fungisida sintetik yang diakui dapat menimbulkan berbagai dampak negatifpada lingkungan maupun kesehatan manusia. Penggunaan ekstrak air rimpang lengkuas untukperlakuan benih dapat diterapkan sebagai alternatif pengendalian penyakit tular benih padakedelai. Lengkuas telah banyak dilaporkan memiliki sifat antijamur dan antibakteri. Penelitian inidilaksanakan dengan tujuan untuk mengetahui keefektifan ekstrak air rimpang lengkuas sebagaiantijamur untuk perlakuan benih dalam mengendalikan penyakit tular benih pada kedelai.Percobaan dilaksanakan di Laboraturium Fitopatologi dan rumah kaca Departemen Hama danPenyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran. Metode penelitian yangdigunakan adalah metode eksperimen dengan pengujian keefektifan air persaan rimpang lengkuasterhadap kemunculan, penekanan perkecambahan konidia, uji pertumbuhan benih, dan viabilitasbenih jamur Colletotrichum spp. pada kedelai. Ekstrak air rimpang lengkuas dengan konsentrasi10%; 30%; 50%; 70%; 90%; dan 100% serta fungsida berbahan aktif metalaksil (0,5 g/l)diaplikasikan sebagai perlakuan benih kedelai. Hasil percobaan menunjukkan ekstrak air rimpanglengkuas konsentrasi 100% dapat menekan sampai 100% kemunculan Colletotrichum spp. setelah perlakuan benih, menekan perkecambahan konidia sebesar 76,20% serta meningkatkanpertumbuhan dan viabilitas benih kedelai.Kata kunci: engkuas, ekstrak air, perlakuan benih, kedelai, Colletotrichum spp.


Author(s):  
A.M. Mahalle ◽  
N.J. Chikhale ◽  
M.N. Mishra ◽  
S.K. Burghate
Keyword(s):  

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