Effect of rhizobial nod factors (lipo­chitooligosaccharide) on seedling growth of blackgram under salt stress

Author(s):  
D. Udhaya Nandhini ◽  
E. Somasundaram ◽  
M. Mohamed Amanullah

A study was carried out to examine the performance of blackgram seedlings under varying levels of salinity and to ascertain the remedial effect of lipo chitooligosaccharide (LCO) against salinity stress. Higher germination percentage, root and shoot length, vigour index and salt tolerance index and lower phytotoxicity of roots and shoots (0) were observed in control (no salinity) treatment irrespective of priming. Salinity negatively influenced the seedling growth and as the salinity increased the seedling growth characters were drastically reduced. The nod factor treated blackgram seedlings had relatively higher germination percentage , root and shoot length, vigour index and salt tolerance index and lower phytotoxicity of roots and shoots. Seed priming with nod factor was found to have significant effect on eliminating the effects of salinity.

Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.


2021 ◽  
pp. 146-149
Author(s):  
V. Sangeetha ◽  
P.S. Sharavannan

The present study is aimed to investigate the effect of tannery effluent on different varieties of Sorghum bicolor. The disparate concentrations of tannery effluent viz., 5, 25, 50, 75 and 100% were tested for its impacts on six varieties of Sorghum bicolor (L.) Moench (TNAU CO 5, TNAU CO 30, CO (S) 28, BSR 1, K Tall and Paiyur 1). The experimentation was carried out in a completely randomized design (CRD) with five replications. The impact of tannery effluent on the germination percentage, shoot length (cm), root length (cm), seedling weight (g), vigour index, tolerance index and phytotoxicity were recorded. The outcome revealed that the parameters escalated in 5% effluent concentration and declined after 25% concentration of tannery effluent.


2018 ◽  
Vol 20 (2) ◽  
pp. 53-66
Author(s):  
E Sultana ◽  
MA Hasan ◽  
S Sikder ◽  
MS Rana ◽  
F Alam

An experiment was conducted to evaluate seedling growth of wheat under saline condition. Thirty wheat genotypes were grown in trays containing different levels of salinity (Control, 6 and 12 dSm-1) during October to December, 2013. Seedling emergence index, shoot and root length, shoot and root dry weight were found to be reduced with the increases of soil salinity level but the degree of reduction were not similar for all wheat genotypes. Salt tolerance index (STI) also indicated a wide difference in salt tolerance among the wheat genotypes. Sourav, Gourav, Shatabdi, BAW 1185, BAW 1186, BAW 1187, BAW 1189 and BAW 1193 were more salt tolerance while BAW 1177, BAW 1190 and BAW 1198 showed greater salt sensitivity than the other wheat genotypes at 6 dS m-1. However, at 12 dS m-1, Sourav, Gourav, Shatabdi, Sufi and BAW 1184 showed more salt tolerance and BAW 1183, BAW 1190, BAW 1192, BAW 1194 and BAW 97 provided greater stress sensitivity among the testing wheat genotypes. Considering both saline stress Sourav, Gourav and Shatabdi were found to be salt tolerant and BAW 1190 was saline sensitive wheat genotypes.Bangladesh Agron. J. 2017, 20(2): 53-66


Agropedology ◽  
2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Ranbeer Singh Chauhan ◽  
◽  
J.S. Chauhan ◽  
A.S. Rawat ◽  
Dinesh Singh Rawat ◽  
...  

Germination ability of seeds varies significantly amongst different varieties of same crop when exposed to the stress. Paddy (Oryza sativa L.) is known to be highly sensitive to salinity during germination and young seedling stages. Present study was aimed to observe the effects of six salinity concentrations (0%, 0.1%, 0.5%, 1%, 1.5% and 2%) on the germination behaviour of two paddy landraces (Chenaphool and Gyasu), frequently grown in Chakrata area of district Dehradun, Uttarakhand. Salinity (>0.1%) was inversely related to final germination percentage, germination energy, plumule dry weight, plumule length, radical dry weight, radical length and speed of germination. Chenaphool landrace showed higher germination and growth (plumule and radical) at 0% salinity (control), while Gyasu landrace at 0.1% salinity level.Dry weight percentage reduction increased with increasing salinity level, while negative correlation was observed between salinity and salt tolerance index and seed vigour index for both the varieties. The study concluded that the long grained, irrigated landrace Chenaphool was more sensitive to salinity in comparison to short grained, non-irrigated landrace Gyassu. Lower salinity conditions have no adverse effects on the germination behaviour of Gyasu landrace.


2010 ◽  
Vol 2 (3) ◽  
pp. 91-95 ◽  
Author(s):  
Alireza PIRZAD ◽  
Vahid GHASEMIAN ◽  
Reza DARVISHZADEH ◽  
Mohammad SEDGHI ◽  
Abbas HASSANI ◽  
...  

A bioassay run was carried out in the incubator to evaluate possible allelopathic effects of water extracts of sage and white wormwood on germination and seedling growth of purslane. Results showed that the type of extract and its concentration (0, 5, 10, 15 and 20%) significantly influenced final germination percentage, germination rate and index, root and shoot length, root/shoot ratio, fresh and dry weight of seedling of purslane. The interaction between these two experimental factors was always significant, producing different results according to the different combination levels. The statistical comparison of means indicated that the maximum germination percentage and germination rate (respectively 68% and 11.4% d-1) were obtained from the untreated control (0% extract), while the lowest values for the same two characters occurred with 15% of sage (respectively 45% and 6.4% d-1) and 20% of white wormwood (38% and 7.5% d-1). Concerning germination indexes, the highest (55) and the lowest (32) values were observed respectively on the control and with 15% of sage extract or 20% of white wormwood extract. The longest (4.5 cm) and the shortest (1.3 cm) root of purslane were obtained with 5% of white wormwood and 20% of sage extracts, respectively. Changes in shoot length with sage and white wormwood extracts were similar to those in root length, even though shoot length was less affected by the concentration of extracts. Root and shoot length changes brought to maximum (22.0) and minimum (8.9) values for the root/shoot length ratio, respectively with 5% of white wormwood and 20% of sage extracts. The maximum fresh (2.111 g) and dry (0.338 g) seedling weight of purslane were obtained from untreated control, producing the same seedling weight with 5% of sage, 5 and 10% of white wormwood extract. While the minimum fresh (0.692 g) and dry (0.111 g) seedling weight were obtained from 15% of white wormwood extract, so that there is no significant differences between this value and the seedling weight produced under 10, 15 and 20% of sage and 20% of white wormwood extract.


2016 ◽  
Vol 44 (1) ◽  
pp. 264-271 ◽  
Author(s):  
Behrooz SARABI ◽  
Sahebali BOLANDNAZAR ◽  
Nasser GHADERI ◽  
Seyed Jalal TABATABAEI

Although salt stress affects all growth stages of a plant, seed germination and seedling growth stages are known to be more sensitive for most plant species. The current research was carried out to find the most effective indicators when screening melons for salt tolerance. For this purpose, a factorial experiment as a completely randomized design was performed in order to investigate the effects of salinity (0, 20, 40, 60 and 80 mM NaCl) on seed germination parameters, hydrogen peroxide content, lipid peroxidation and some antioxidant enzymes (guaiacol peroxidase, ascorbate peroxidase and superoxide dismutase) in five Iranian melon landraces and one melon cultivar ‘Galia’ F1 at early seedling growth stage. The ‘Galia’ F1 was included for comparison as a salt-tolerant cultivar. The data collected from all salinity treatments across six landraces were used for analysis of variance and principal component analysis (PCA) using SPSS software as well as for obtaining Pearson’s correlation coefficients (r) among all physiological parameters. Obtained results indicated that averaged all landraces across all treatments, increased NaCl significantly affected all the parameters measured. In all landraces, salt tolerance index (STI) decreased as the salt concentrations increased. The principal component analysis revealed variations among the traits and determined four main factors that explained 97.53% of the total variance. Simple correlation coefficient analyses showed the existence of significant positive and negative correlations among characteristics. Our study suggests that the salt tolerance index and principal component analysis can be used as accurate and easy indicators when screening melons for salt tolerance.


Author(s):  
C. R. Mohanty ◽  
A. Mohanty ◽  
R. Parhi

A field experiment on the effect of planting dates and pinching on seed production in African marigold cv. Sirakole was carried out during November, 2007 to June, 2008 with four planting dates and three levels of pinching. November planting gave more number of seeds (191.89) and weight of seed per head (0.32g), seed yield per plot (272.24 g), 1000 seed weight (1.65g) as well as seed yield (1008.29 kg) per hectare. In addition to these quality traits like germination percentage of seed, root and shoot length of seedlings and vigour index also witnessed significant improvement under this date of planting. Shoot pinching at 30 days after planting was effective in bringing significant improvement in number (173.01) and weight (0.25g) of seeds per head as well as seed yield (309.77 kg) per hectare. Interaction effect of November planting and single pinching improved germination percentage (90.44) and seed vigour index (2098).


2016 ◽  
Vol 35 (3) ◽  
pp. 213-223 ◽  
Author(s):  
Taoufik El Rasafi ◽  
Mohamed Nouri ◽  
Said Bouda ◽  
Abdelmajid Haddioui

AbstractThis study was conducted to evaluate the effect of metals on wheat and bean species. The method uses seed germination and early seedling growth of these plants in the presence of various levels (10, 50, 100, 250, 500, 750 and 1000 mg/L) of Cadmium (Cd), Iron (Fe) and Zinc (Zn). The inhibition caused by these metals was depending on the concentration used, the metal itself and the plant species. The species had reduced seed germination, root and shoot lengths, tolerance index and percentphyto-toxicity with increasing concentrations of metals. Cadmium was determined to be the most inhibitory metal on these parameters. This metal affected significantly the germination, root and shoot length of the species tested, as well as the tolerance index and percentphytotoxicity starting from 50 mg Cd/l. Under the Iron stress, in general, the inhibition of germination and root length of wheat was reduced from 500 mg Fe/l. The results showed also that the inhibitory effect of increase of Zn levels was seen in root, shoot and tolerance indices. The findings also revealed that the metal toxicity was as follow: Cd > Fe > Zn. Regarding species, the results showed that bean seemed to be more tolerant to the increase of the three metals than wheat.


2020 ◽  
Vol 26 (1) ◽  
Author(s):  
A.C Vikhe ◽  
J. N. Nehul

The present experimental work has been done on Vigna radiata, Cultivar Naval. The effects of Chemicals and Physical Mutagens were studied on seedling development. The EMS, SA and Gamma Radiation affected the seedling growth at the time of development on its root and shoot. Seeds were treated with various concentrations of Chemical Mutagens and Gamma Radiation. The result showed variation in Root and Shoot Length, Vigour index (Length, Mass), Dry weight as compared to control.


2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.


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