Seroprevalence and risk factors of bovine brucellosis in the province of Djelfa (ALGERIA)

2018 ◽  
Author(s):  
A. Yahia ◽  
K. Hamrat ◽  
K. Saidani ◽  
R. Kaidi
Keyword(s):  
Risk Factors ◽  
Epidemiological Study ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Age Groups ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Factors Affecting ◽  
Significant Difference

In order to determine the prevalence of bovine brucellosis and the factors affecting its persistence in the province of Djelfa (Algeria), a sero-epidemiological study was carried out on a total of 10827 cattle (7346 females and 3481 males) during the decade from 2004 to 2013. The sera were screened by card agglutination test, and the positive samples were confirmed by the complement fixation test. Some factors (year, age, breed and sex) were considered. Results found an average prevalence of brucellosis during these ten years of 1.4% with a highly significant difference according to year and sex (P Lass Than 0.05). A prevalence of 1.58% for females against a prevalence of 1.03% for males was observed. No significant difference was observed for the different age groups (P>0.05) in the seroprevalence of brucellosis. A higher prevalence of infection (1.53%) was found in imported breeds compared to local and cross breeds with (1.39%) and (1.35%) respectively. The effect of the breed is highly significant (P Lass Than 0.05). In conclusion the bovine brucellosis persists in the province of Djelfa and it is necessary to implement a scheme to fight and prevent this infection.

scholarly journals Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan
Keyword(s):  
Specific Antibody ◽  
Brucella Abortus ◽  
Bovine Serum ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Serum Agglutination

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

Studies on resistance of sheep to infestation with Haemonchus contortus and Trichostrongylus spp. and on the immunological reactions of sheep exposed to infestation. I. The preparation of antigens for the complement fixation test and the reactivity of the biochemical fractions of H. contortus

1950 ◽  
Vol 1 (3) ◽  
pp. 285 ◽  
Author(s):  
DF Stewart
Keyword(s):  
Haemonchus Contortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Mature Adult ◽  
Infective Larvae ◽  
Lipid Fractions ◽  
Significant Difference ◽  
Third Stage ◽  
Free Material

All antigen was developed to detect circulating antibodies by means of the complement fixation test in sheep infested with Haemonchus contortus and Trichostrongylus spp. Extraction of worm material at 100°C. for 10 minutes was found to be the most satisfactory method for the preparation of antigens. Potent antigens were prepared from young adult H. contortus, from third-stage infective larvae, and from the eggs. Old mature adult H. contortus yielded antigens of low potency. No significant difference was found between the potency of antigens prepared from male and female adult H. contortus collected from the same sheep. Both adult Trichostrongylus spp. and third-stage infective larvae consistently yielded antigens of high potency. No significant difference was found in the results obtained with larval or adult H. contortus antigens, adjusted to the same potency and tested with natural H. contortus antisera. H. contortus and Trichostrongylus spp. absorbed the antiserum to each other. It was shown that a lipid was an essential constituent of the boiled antigen in the complement fixation reaction with natural antisera. Lipid-free antigens from H. contortus failed to react with natural antisera. The lipid was not antigenic when injected into rabbits. The carbohydrate fraction of H. contortus did not fix complement in the presence of natural antisera and was not antigenic when injected into rabbits. The lipid fraction of a variety of nematode parasites reacted with natural antisera to H. contortus infestation. Similar lipid fractions of two species of trematodes did not react with natural antisera to H. contortus infestation. Normal saline suspensions of the lipid-free material from a variety of helminths showed a greater degree of specificity when tested with artificially prepared antisera than did the lipid fractions.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

1983 ◽  
Vol 90 (1) ◽  
pp. 67-70 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Supporting Evidence ◽  
Bovine Brucellosis ◽  
Eradication Programme ◽  
Female Cattle ◽  
The Rose ◽  
Supplementary Test ◽  
Haemolysis Test

SUMMARYSera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with Brucella abortus strain 19 was compulsory. Of 4583 sera positive by the Rose Bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. These figures, and supporting evidence from the eradication programme, suggest that the radioimmunoassay may be a useful supplementary test in problem herds.

scholarly journals Detection of brucellosis in cattle and evaluation of risk factors associated with the disease in workers slaughterhouse

2021 ◽  
Vol 10 (3) ◽  
pp. e39710313248
Author(s):  
Thaise Marques Alves ◽  
Poliana de Castro Melo ◽  
Lilia Marcia Paulin Silva ◽  
Nathana Kyolla Santos de Carvalho ◽  
Amora Ferreira Menezes Rios ◽  
...  
Keyword(s):  
Risk Factors ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
State Inspection ◽  
Agglutination Test ◽  
The State ◽  
Antigen Test ◽  
Factors Associated ◽  
Inspection Service ◽  
Smooth Strain

Brucellosis is anthropozoonosis caused by Brucella spp. Among the zoonotic species, B. abortus is the main species affecting cattle and can easily be transmitted to humans. The purpose of this study was to investigate, through epidemiological inquiry and serological analysis,  animal and human health as related to smooth strain Brucella spp. in a slaughterhouse located in the southern region of the state of Bahia. For this purpose, blood samples were collected from workers and animals at a slaughterhouse together with the State Inspection Service. Then, the Buffered Acidified Plate Antigen test was performed for animals and humans, the Slow Agglutination Test was performed for humans only; and the Complement Fixation Test and the 2-Mercaptoethanol Brucella Agglutination test (2ME) were performed for animals only. In addition, an epidemiological inquiry was applied to workers in order to assess risk factors for the disease. After data analysis, it was concluded that infection by smooth strains of Brucella spp. was detected in 14.0% of the cattle. Additionally, one worker out 41 tested reactive to the disease.

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