Assessment of zinc and lead concentration in water and muscles of Labeo rohita collected from wild and local fish farms of Pakistan

Author(s):  
Khalid Mahmood Anjum ◽  
Atif Yaqub ◽  
Ehsan Mehmood Bhatti ◽  
Muhamad Yameen ◽  
Muhammad Sharif Mughal ◽  
...  

Reasonable evidences suggest that heavy metal pollutants have been added by factories and other industries to our local water bodies. The present study was conducted to determine the heavy metal (zinc and lead) concentrations in muscle tissues of Labeo rohita inhabiting local water bodies as well as those being raised at farms. Sampling was performed to collect Labeo rohita from two different stations along river Ravi, viz. Lahore Siphon (upstream) and Balloki Headworks (downstream). Additionally, samples were also obtained from the government operated as well as private fish farms located in Lahore District, Pakistan. The fish were dissected; their muscles were digested, filtered, and finally analyzed for concentrations of Zn and Pb. Significant variation of heavy metal ions were observed between two sites along river Ravi as well as government operated and private fish farms. Significantly high mean concentrations of Zn (10.7±1.268) and Pb (0.61±0.082) were observed at Balloki Head-works; whereas, the lowest Zn (1.58±0.235) and Pb (0.16±0.008) heavy metal levels were recorded in the muscle samples collected from private fish farms. It was also observed that the heavy metal concentration in the muscles of Labeo rohita, collected from Balloki Head-works was much higher than the permissible limits laid down by WHO/FAO. Further the recorded highest level of heavy metals in water samples collected from River Ravi indicates that the bioaccumulation of heavy metals is severely affecting the aquatic life of fresh water and industries should not be allowed to release their effluent directly into the river Ravi in their vicinity.

2009 ◽  
Vol 3 (2) ◽  
pp. 48-64
Author(s):  
Kadhim M. Ibrahim ◽  
Shaimaa A. Yousir

Several experiments were carried out to study heavy metal tolerance in tissue cultures or whole plants of S. grandiflora., Callus was induced and maintained on modified Murashige and Skoog, 1962 medium (MS) supplemented with (0.5)mg/l benzyl adenine and (2)mg/l 2,4-phenoxy acetic acid . Heavy metals (Cd, Co, Cu, Cr or Zn) were added to the culture medium at different concentrations as contamination agents. In order to asses the effect of these heavy metals on seed germination; seeds were sown in soil contaminated with different concentrations of heavy metals for 3 weeks. Atomic Absorption Spectrophotometer was used for analysis of samples taken from whole plants and callus cultures. Results showed that callus fresh weight decreased with increasing heavy metal concentration in cultural medium. Germination percentages and plant heights increased over time. However, a reduction occurred in these parameters with increasing heavy metal levels. Percentages of metals accumulated in calli were (0.001, 0.011, 0.012 and 0.013%) at (0.0, 0.05, 0.075 and 0.1)mg/l Cd respectively; (0.001, 0.008, 0.016 and 0.006%) at (0.0, 0.1, 0.25 and 0.5)mg/l Co respectively; (0.001, 0.020, 0.034 and 0.015%) at (0.0, 0.075, 0.2 and 0.5)mg/l Cu respectively; (0.001, 0.013, 0.012 and 0.010%) at (0.0, 0.25, 0.4 and 0.5)mg/l Cr respectively and (0.027, 0.051, 0.059 and 0.056%) at (0.0 , 0.75, 1.0 and 1.5)mg/l Zn respectively. Percentages of metals accumulated in whole plants were (0.08, 0.55, 1.11, 0.83 and 0.44%) at (0.0, 1.0, 2.0, 3.0 and 4.0)mg/Kg soil Cd respectively; (0.11, 0.22, 0.55, 0.47 and 0.44%) at (0.0, 15.0, 30.0 45.0 and 60.0)mg/Kg soil Co respectively; (0.01, 0.10, 0.57, 0.58 and 0.72%) at (0.0, 25.0, 50.0, 75.0 and 100.0)mg/Kg soil Cu respectively. (0.08, 0.80, 1.28, 1.31 and 0.88%) at (0.0, 25.0, 50.0, 75.0 and 100.0)mg/Kg soil Cr respectively and (0.06, 1.11, 1.20, 1.83 and 2.22%) at (0.0, 100.0, 200.0, 300.0 and 400.0)mg/Kg soil Zn respectively.


Author(s):  
D. M. Abbey ◽  
M. E. Abbey ◽  
T. C. Ogunyemi ◽  
M. E. Abbey

Evaluation of physicochemical parameters and heavy metal levels in surface water of the Miniokoro stream, Port Harcourt, Nigeria were investigated to determine its potability and pollution status at various stations along the stream.  The physicochemical parameters analyzed in the water samples were: pH, Temperature, Salinity, Electrical conductivity, TDS, DO, BOD, Alkalinity, Chloride, NH4+, NO3-, PO43-, SO42- while heavy metals such as Nickel, lead, Iron, Zinc and Chromium were analyzed using atomic absorption spectrophotometer. The results from the research showed an average concentration level of the physicochemical parameters such as pH (5.79-7.01), EC (136.43 - 960.67µS/cm), TDS (90.17 - 1517.67) and the spatial distribution of heavy metals: zinc (0.07 - 1.09mg/l), chromium (0.03 - 0.09mg/l), nickel (0.07 - 0.08mg/l) and iron (12.29 - 39.95mg/l). Water samples from various stations depicts the varying level of pollution along the stream when compared with national and international permissible limit. Thus, it suggests that the government should develop and implement an effective waste management plans due to environmental and other anthropogenic impacts on the stream.


2017 ◽  
Vol 10 (1) ◽  
pp. 21-24
Author(s):  
MS Islam ◽  
SC Shil ◽  
MH Kabir ◽  
ME Hoq

The study was conducted to investigate the heavy metal contamination in fishes and shrimps from the Passur River near the world famous Sundarban mangroves located in the southwestern part of Bangladesh, during the period from January to June 2013. The fish (Liza parse) and shrimp (Penaeus monodon) samples were collected from two stations as St-1 (Katakhali-1 km upper from port area- upstream) and St-2 (Amtola-1 km lower from port area- downstream) monthly for three months in January (winter), March (pre monsoon) and May (Monsoon). Before analysis, a bit of muscle, the whole liver and two gill arches from each fish and shrimp were removed. Samples of tissues from each fish and shrimp were removed, weighed and dried. A microwave digestion system was used to prepare the samples for analysis. After digestion residue diluted with 25 ml 2.5% HNO3. The water used was deionized and distilled. The metal analyses of samples (Cd, Cr, Cu, Pb and Zn) were carried out by using UNICAM-929 atomic absorption spectrophotometer (AAS) in SGS Bangladesh Limited, Dhaka-1205, Bangladesh. Among the heavy metals studied Cd, Cr, Cu, Pb and Zn were detected in fish and shrimp species were within acceptable limits by FAO standards. The results of this study indicated that a general absence of serious pollution in the Passur River is due to heavy metals; whereas the concentrations of elements found could mainly be attributed to geological sources. In this regard, to maintain the water and sediment qualities as well as the sound and safe aquatic life of the river need proper monitoring and management by the relevant authorities of the Government of Bangladesh.J. Environ. Sci. & Natural Resources, 10(1): 21-24 2017


F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1239 ◽  
Author(s):  
Asus Maizar Suryanto Hertika ◽  
Kusriani Kusriani ◽  
Erlinda Indrayani ◽  
Rahmi Nurdiani ◽  
Renanda B. D. S. Putra

Background: The objective of this study was to compare the levels of heavy metals (Pb, Hg, and Cd) and metallothionein (MT) in the gills and stomach of two species of mussels (Crassostrea iredalei and Crassostrea glomerata), and to observe the ability of the mussels to absorb the heavy metals Pb, Hg and Cd present in the water. Methods: The mussels were obtained from Mayangan, Kenjeran and Gresik ports, East Java, Indonesia. MT levels were determined using ELISA. Heavy metal levels of Pb, Hg and Cd were assayed using atomic absorption spectrophotometry. Results: The levels of Pb and Cd in water were below the maximum permissible levels for local water quality standards. By contrast, the level of Hg in the water was above the maximum permissible levels for water quality standards. At Mayangan Port (Station 1), the level of Pb was higher than Hg and Cd. Levels of MT and heavy metals varied greatly among of C. iredalei and C. glomerata individuals, but were always higher in the gills than in the stomach. The highest MT level (160,250 ng/g) was observed at Kenjeran Port (Station 2). MT levels were shown to be significantly associated with heavy metal level (P<0.0001). Conclusions: This result indicates that MT may be responsible for the sequestration of these heavy metals, as has already been observed in terrestrial animals.


2020 ◽  
Vol 12 (2) ◽  
pp. 114-122
Author(s):  
Wahyu Irawati ◽  
Aaron Hasthosaputro ◽  
Lucia Kusumawati

The increasing industrial activity in Indonesia, that is not equipped with appropriate waste treatment, has caused an increase of heavy metal contaminants in water bodies. Heavy metals contamination such as copper (Cu), mercury (Hg), cadmium (Cd), and lead (Pb) contamination in water bodies have endangered aquatic life and public health. For this reason, it is urgently important to lower down the concentration of heavy metal pollutants in the water bodies surrounding industrial areas. Compared to chemical remediation, bioremediation of heavy metal by using indigenous bacteria is more effective and economical, since it can be applied in situ directly and be used repeatedly. Acinetobacter sp. IrC2, used in this study, is Indonesian indigenous bacteria isolated from the industrial waste treatment facility in Rungkut, Surabaya. This study aims, firstly, to investigate the heavy metal multiresistance of Acinetobacter sp. IrC2 against mercury, cadmium, and lead. Secondly, this study intends to examine its bioaccumulation capacity for single and heavy metal alloys. The heavy metal multiresistance test was carried out by measuring the minimum heavy metal concentrations that inhibit bacterial growth (Minimum Inhibitory Concentration/MIC). The bioaccumulation capacity was measured using an atomic absorption spectrophotometer (AAS).  It is shown that Acinetobacter sp. IrC2 has high multiresistance to mercury, cadmium, and lead with MIC values of 12 mM, 8 mM, and 18 mM, respectively. Furthermore,  it is also resistant to  heavy metal mixture of 4.5 mM.  The mechanism of bacterial resistance in response to heavy metal toxicity, in general, is by accumulating heavy metals in the cells. The highest amount of accumulated heavy metals identified, from bacteria grown in the medium contains a mixture of heavy metals, were 0.023 mg, 0.084 mg, 0.684 mg, and 1.476 mg per gram of cell dry weight for copper, mercury, cadmium and lead respectively.  In conclusion, Acinetobacter sp. IrC2 is a promising heavy metal bioremediation agent due to its heavy metal multiresistance and accumulator characteristics.  Key words: Acinetobacter sp. IrC2; cadmium; copper; lead; merkuri


Author(s):  
Ngo The Cuong ◽  
Tran Hoan Quoc ◽  
Svetlana Vasilievna Zolotokopova

The article focuses on the study of change of containing heavy metals (zinc, copper, iron, cadmium, lead, arsenic) in the abiotic and biotic components of the Serepok river (Vietman) influenced by wastewater discharge from industrial areas. Heavy metal content was determined in the river water and bottom sediments in the four zones: above and within the boundaries of industrial regions Xoa Phu and Tam Thang and in two water reservoirs situated below the boundaries of those industrial areas. Tilapia Galilean ( Sarotherodon galilaeus ), Hemibagrus ( Hemibagrus ), and sazan ( Cyprinus carpio ) caught in these areas were the hydrobionts under study in which liver, gills, skeleton and muscles accumulation of heavy metals was detected. In the organs of fish caught in the river within industrial region, heavy metals concentration was 3-7 times higher. The greatest concentration of heavy metals was found in the liver and gills of fish caught in the boundaries of industrial regions, the least concentration was in the muscles. In most cases, significant correlation between heavy metal concentration in organs of fishes and in river water, bottom sediments has been revealed.


Author(s):  
MdDidarul Islam, Ashiqur Rahaman, Aboni Afrose

This study was based on determining concentration of essential and toxic heavy metal in coconut water available at a local Hazaribagh area in Dhaka, Bangladesh. All essential minerals, if present in the drinking water at high concentration or very low concentration, it has negative actions. In this study, fifteen samples and eight heavy metals were analyzed by Atomic Absorption Spectroscopy (AAS) method which was followed by wet ashing digestion method. The concentration obtained in mg/l were in the range of 0.3 to 1.5, 7.77 to 21.2, 0 to 0.71, 0 to 0.9, 0 to 0.2, 0.9 to 17.3, 0.1 to 0.9, 0 to 0.9 and 0 to 0.7 for Fe, Ni, Cu, Cd, Cr, Zn, Pb and Se respectively. From this data it was concluded that any toxic heavy metals like Cd, Cr, Pb and Ni exceed their toxicity level and some essential nutrients were in low concentration in those samples. 


2018 ◽  
Vol 69 (7) ◽  
pp. 1695-1698
Author(s):  
Marin Rusanescu ◽  
Carmen Otilia Rusanescu ◽  
Gheorghe Voicu ◽  
Mihaela Begea

A calcium bentonite from Orasu Nou deposit (Satu Mare Romania) was used as raw material. We have conducted laboratory experiments to determine the influence of bentonite on the degree of heavy metal retention. It has been observed that the rate of retention increases as the heavy metal concentration decreases. Experimental studies have been carried out on metal retention ( Zn) in bentonite. In this paper, we realized laboratory experiments for determining the influence of metal (Zn) on the growth and development of two types of plants (Pelargonium domesticum and Kalanchoe) and the effect of bentonite on the absorption of pollutants. These flowers were planted in unpolluted soil, in heavy metal polluted soil and in heavy metal polluted soil to which bentonite was added to observe the positive effect of bentonite. It has been noticed that the flowers planted in unpolluted soil and polluted with heavy metals to which bentonite has been added, the flowers have flourished, the leaves are still green and the plants whose soils have been polluted with heavy metals began to dry after 6 days, three weeks have yellowish leaves and flowers have dried. Experiments have demonstrated the essential role of bentonite for the removal of heavy metals polluted soil.


2016 ◽  
Vol 5 (10) ◽  
pp. 4933
Author(s):  
Sabia Sultana ◽  
A. K. M. Nur Alam Siddiki ◽  
Md. Rokonujjaman ◽  
M. Niamul Naser ◽  
Abdus Salam ◽  
...  

The heavy metal concentration (e.g., Mn, Zn, Pb and Ni) were determined in soft tissues and shells of freshwater mussels (Lamellidens marginalis) at the various sites of Dhanmondi lake, Dhaka, Bangladesh between the period April, 2010 and March 2011. The heavy metal concentrations in shells and soft tissues of freshwater mussels were tended to vary significantly among sampling points and seasons in Dhanmondi Lake. Distribution of heavy metals in shell and soft tissue of Lamellidens marginalis followed the order Mn>Zn>Pb>Ni, respectively. According to the t-test, level of manganese, zinc and lead under investigation between shell and tissue showed statistically significant differences [Mn: t=-11.387; df=16; P=0.000; Zn: t=-2.590; df=16; P=0.020 and Pb: t=-2.8679; df=16; P=0.011].


2021 ◽  
Vol 11 (15) ◽  
pp. 7099
Author(s):  
Inkyeong Moon ◽  
Honghyun Kim ◽  
Sangjo Jeong ◽  
Hyungjin Choi ◽  
Jungtae Park ◽  
...  

In this study, the geochemical properties of heavy metal-contaminated soils from a Korean military shooting range were analyzed. The chemical behavior of heavy metals was determined by analyzing the soil pH, heavy metal concentration, mineral composition, and Pb isotopes. In total, 24 soil samples were collected from a Korean military shooting range. The soil samples consist of quartz, albite, microcline, muscovite/illite, kaolinite, chlorite, and calcite. Lead minerals, such as hydrocerussite and anglesite, which are indicative of a transformation into secondary mineral phases, were not observed. All soils were strongly contaminated with Pb with minor concentrations of Cu, Ni, Cd, and Zn. Arsenic was rarely detected. The obtained results are indicated that the soils from the shooting range are contaminated with heavy metals and have evidences of different degree of anthropogenic Pb sources. This study is crucial for the evaluation of heavy metal-contaminated soils in shooting ranges and their environmental effect as well as for the establishment of management strategies for the mitigation of environmental risks.


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