Testicular Biometry for Breeding Soundness Evaluation of Sahiwal Males Reared under Tropical Conditions

Author(s):  
Muzamil Abdullah ◽  
T.K. Mohanty ◽  
M. Bhakat ◽  
A. Kumaresan ◽  
A. Singh ◽  
...  

Background: The testicular biometry such as scrotal circumference (SC) and scrotal volume, affect semen quality hence bull fertility and thus testicular biometry can be used for breeding soundness of bulls. Methods: The present study was carried out to study the testicular parameters of 77 Sahiwal males of different ages and to establish its importance for selection of breeding bulls during 2015-2016 at ICAR-NDRI, Karnal. The testicular biometry of each male was done at monthly intervals using Vernier calliper, cloth tap and ultrasonography. Result: The semen quality parameters were significantly (P less than 0.05) more in the group having higher scrotal circumference. The scrotal circumference follows the curvilinear manner of growth from 12 months to greater than 72 months (correlation value of 0.92 and R2 0.84). The paired testicular volume (PTV) differed significantly (P less than 0.05) between different age group males. Scrotal circumference and paired testicular volume are directly related to semen quality hence bull fertility. Thus, testicular biometry is an important criterion for breeding soundness for selecting Sahiwal males. 

2018 ◽  
pp. 7097-7103
Author(s):  
Liliana Chacón ◽  
Harvey Lozano Marquez ◽  
Jaumer Andrés Orozco Clavijo ◽  
Ariosto Ardila Silva

Objective. Aimed to describe the characteristics of the onset of puberty in males in Colombian hair ram lambs (Ovino de Pelo Colombiano, OPC), and their crosses with Katahdin and Santa Inés sheep in a farm located in Villavicencio, Meta. Materials and methods. 15 lambs of three biotypes: OPC x OPC, Santa Ines x OPC (SO) and Katahdin X OPC (KO) from four months of age, five per group. Ram lambs were grazing and they had supplementation with commercial salt and water ad libitum. Monthly body weight, testicular measurement, scrotal circumference (CE) were measured and serum testosterone were determined by Elisa test, an ultrasound of the two testicles was performed to determine the presence of the testicular mediastinum, also evaluating the presence and detachment of the urethral prolongation and then electro ejaculation was performed to determine the macroscopic and microscopic characteristics of the semen. Results. Genotype effect was significant for evolution of body development (BW, SC, TV) over time, Santa Ines and Katahdin crosses presented the highest values in BW during the investigation whereas OPC lambs maintained a lower value until the end of the trial. At least four evaluations were made in the males or until the presentation of a minimum concentration of 150 million of sperm per ml with 30% of individual progressive motility.  Conclusions.Around six months old is the puberty onset for ram lambs under tropical conditions, in a non-seasonal country, based on a diversity of variables including body development, semen quality, and testosterone levels among others.


2015 ◽  
Vol 3 (12) ◽  
pp. 677-684
Author(s):  
Md. Mahbubur Rashid ◽  
Md. Azharul Hoque ◽  
Khan Shahidul Huque ◽  
Abul Kashem Fazlul Haque Bhuiyan

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 31-32
Author(s):  
Jarret A Proctor ◽  
Nathan S Long ◽  
Connor K Biehler ◽  
Liesel G Schneider ◽  
Justin D Rhinehart ◽  
...  

Abstract Growing concern over breeding soundness examination failures has led to increased interest in identifying factors that may influence semen quality of developing bulls. An experiment was conducted to evaluate relationships between semen quality and scrotal circumference, adiposity, and rumen conditions of Angus bulls (n = 48; 332 ± 47 d of age) that were developed over an 84-d period. Bulls underwent breeding soundness examinations at each of four 21-d intervals. Backfat thickness was measured on d 0 and 84, and rumen pH and temperature were continuously monitored throughout the experiment via rumen boluses (eBolus; eCow, Devon, UK). Simple linear regression was conducted (JMP 13.0; SAS Institute Inc., Cary, NC) to determine independent relationships between semen characteristics and respective explanatory variables. Change in scrotal circumference between d 0 and 84 was directly related to change in percent morphologically-normal sperm (R2 = 0.12; P < 0.05), but no other semen quality characteristics (P ≥ 0.17). Change in backfat thickness throughout the experiment tended to be inversely related to change in percent morphologically-normal sperm (R2 = 0.06; P < 0.10), but was directly related to changes in semen volume (R2 = 0.10; P < 0.05) and total sperm per ejaculate (R2 = 0.12; P < 0.05), but not change in motility (P = 0.24). Mean rumen pH tended to be directly related to changes in semen volume (R2 = 0.07; P < 0.10) and total sperm per ejaculate (R2 = 0.07; P < 0.10), but not changes in motility (P = 0.28) or percent morphologically-normal sperm (P = 0.94). Mean rumen temperature was not related to any semen quality characteristics (P ≥ 0.15). Collectively, these results suggest that rumen pH, as well as changes in scrotal circumference and adiposity are related to and independently explain variation in semen quality of developing bulls.


2014 ◽  
Vol 54 (5) ◽  
pp. 569
Author(s):  
S. J. Liu ◽  
J. L. Li ◽  
H. Li

The objective of this study was to score reactions to artificial semen collection procedures and to develop an efficient method for gander selection in two original geese breeds. One-hundred and three Zi geese and 110 Rhin geese were used. Gander reaction scores to massage and semen quality parameters were investigated in 10 Zi and 10 Rhin ganders during a complete reproductive season. The relationships between average gander massage reaction score and semen quality were evaluated. After natural mating, egg fertility comparison between Zi ganders selected by massage reaction scores and those not subjected to selection were investigated. Ganders exhibited large variations in artificial semen collection and semen characteristics between breeds. Only 30.1% (31/103) of Zi geese and 46.3% (51/110) of Rhin geese had stable positive massage reactions with semen ejaculation (P < 0.05). Average massage reaction score, ejaculate volume, spermatozoa concentration, sperm motility and semen quality factor for single ejaculate were significantly higher in Zi than in Rhin geese. There was a high correlation between average massage reaction score and semen volume, being 0.87 for Zi geese (P < 0.05) and 0.82 for Rhin (P < 0.05), and this correlation enabled pre-selection of ganders at the beginning of the breeding season. Reproductive efficiency could be improved with massage-selected ganders in natural breeding flocks. Examination of gander massage reaction scores combined with laboratory assessment of semen quality early in the breeding season is a feasible method for predicting fertilising capability of ganders.


2010 ◽  
Vol 88 (4) ◽  
pp. 1215-1219 ◽  
Author(s):  
A. A. Boligon ◽  
J. A. V. Silva ◽  
R. C. Sesana ◽  
J. C. Sesana ◽  
J. B. Junqueira ◽  
...  

1999 ◽  
Vol 42 (5) ◽  
pp. 459-468
Author(s):  
P. Póti ◽  
S. Bedó ◽  
M. Mézes ◽  
J. Tózser

Abstract. Scrotal circumference, testosterone content of blood serum, libido and sperm quality parameters of Hungarian Merino breeding and young rams were examined according to seasons by the authors in a farm. In the case of matured rams a seasonal effect was observed both in scrotal circumference and testosterone content of blood serum (measured two hours later after exogenous GnRH treatment) and sperm quality. In the case of young rams no seasonal effect was observed. It was found that the scrotal circumference of young rams and the testosterone content of blood serum (two hours after exogenous GnRH treatment) are determined by the maturity of the rams. A medium connection could be observed between scrotal circumference and testosterone content of blood serum induced by GnRH treatment. In the case of matured rams there is a loose and not significant correlation between scrotal circumference and sperm quality (r = 0.39). They have come to the conclusion that libido shows medium correlation with scrotum circumference (r = 0.56), while the correlation with testosterone content of blood serum is tight (r = 0.78), and the correlation with sperm quality proves to be a loose one. According to the results scrotum circumference can be used as one ofthe possible markers for selection of rams for reproduetion purposes.


2018 ◽  
Vol 16 (2) ◽  
pp. e0403 ◽  
Author(s):  
Melissa Carvajal-Serna ◽  
Jaime A. Cardozo ◽  
Henry Grajales-Lombana ◽  
José A. Cebrián-Pérez ◽  
Teresa Muiño-Blanco

We tested the hypothesis that sheep breed can influence the sperm quality and seminal plasma (SP) composition and investigated any potential relationship between SP proteins and antioxidant enzyme activities (AO) with sperm quality. Ejaculates from twelve rams of three breeds were obtained during the rainy season at high altitudes, and sperm quality was automatically evaluated (CASA-Hamilton Thorne). The AO of superoxide dismutase, glutathione peroxide and glutathione reductase (GR) in SP was evaluated and total proteins were separated by SDS-PAGE. Comparative analyses of semen quality parameters between breeds revealed that Creole and Hampshire breeds had a higher sperm quality compared with Romney Marsh (p<0.05), although no difference in AO was found. GR activity was negatively correlated (p<0.05) with several kinematic variables and positively (p<0.05) with morphological abnormalities. The highest SP protein concentration was found in semen collected from Hampshire males compared with that from Creole and Romney Marsh (p<0.05). SDS-PAGE analysis showed the presence of 32 protein bands in SP with molecular weights between 334 and 10 kDa. Differences (p<0.05) between breeds in bands of 43, 25, 22 and 20 kDa were observed. These results evidence a relationship between the protective effect of the antioxidant enzyme system in SP of three ram types under high altitude and tropical conditions and semen quality. Our findings also suggest that the identified proteins might play an important role in sperm physiology and quality.


2013 ◽  
pp. 17-20
Author(s):  
Csilla Budai ◽  
János Oláh ◽  
István Egerszegi ◽  
András Jávor ◽  
András Kovacs

The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.


2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70


Sign in / Sign up

Export Citation Format

Share Document