Prevalence of antibodies to Bovine Viral Diarrhea Virus (BVDV) in blood and milk serum in dairy cattle in Kars district of Turkey

2016 ◽  
Author(s):  
Volkan Yilmaz
Keyword(s):  
Blood Serum ◽  
Bovine Viral Diarrhea Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Viral Diarrhea ◽  
Dairy Herds ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Milk Serum ◽  
Viral Diarrhea Virus

The purpose of this study was to detect of antibodies against Bovine Viral Diarrhea Virus (BVDV) in blood and milk serum samples. With this aim, a total of 192 blood and milk samples were collected from unvaccinated Holstein cows in Kars district of Turkey. Blood and milk serum samples were tested to determine the presence of antibodies against BVDV by commercial indirect enzyme-linked immunosorbent assay (ELISA). In this study, while 172 of blood serum (89.58%) were found to be positive for the presence of BVDV antibodies, 161 of milk serum samples (83.85%) were positive. In addition, 150 (78.12%) both blood and milk serum samples of same cattle were positive for BVDV antibodies. Only 22 (11.45%) blood serum of cattle was detected positive for BVDV, while only 11 (5.73%) milk serum was seropositive. Data obtained from the study showed the presence of BVDV infection in dairy herds in the Kars region and demonstrate that blood serum and milk serum samples might be consistent with one another in the determination of BVDV seroprevalence.

scholarly journals Seroprevalence of Bovine Viral Diarrhea on Smallholder Farms on the Territory of Belgrade City

2021 ◽  
Vol 71 (3) ◽  
pp. 318-325
Author(s):  
Zorana Zurovac Sapundžić ◽  
Milan Ninković ◽  
Dimitrije Glišić ◽  
Bojan Milovanović ◽  
Jadranka Žutić ◽  
...  
Keyword(s):  
Blood Serum ◽  
Enzyme Immunoassay ◽  
Bovine Viral Diarrhea Virus ◽  
Viral Disease ◽  
Bovine Viral Diarrhea ◽  
Serum Samples ◽  
Smallholder Farms ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Abstract Bovine viral diarrhea (BVD) is a viral disease of cattle with a high economic impact. To estimate the seroprevalence of Bovine viral diarrhea virus (BVDV) infection of cattle on smallholder farms we included 78 smallholder farms in the Belgrade epizootiological area where 318 blood serum samples from cattle were collected, and subsequently tested. The samples were analyzed using a commercially available competitive enzyme immunoassay (ELISA) for the detection of antibodies against BVDV. The obtained results showed an overall seroprevalence of 3.8% whereas the seroprevalence on herd level varied from 0% to 80%. The obtained results showed a relatively low seroprevalence of BVD infection on smallholder farms on the territory of Belgrade city.

scholarly journals Is contamination of bovine-sourced material with bovine viral diarrhea virus still a problem in countries with ongoing eradication campaigns?

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Aleksandra Antos ◽  
Jerzy Rola ◽  
Michał Bednarski ◽  
Michał Konrad Krzysiak ◽  
Julia Kęsik-Maliszewska ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Genetic Material ◽  
Elisa Test ◽  
Bovine Viral Diarrhea ◽  
Rt Pcr ◽  
Serum Samples ◽  
Viral Pathogens ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

AbstractIn this report, we describe the detection of bovine viral diarrhea virus (BVDV) contamination in commercial animal-derived sera and vaccines against animal viral pathogens on the market in Poland. Antibodies against BVDV were detected in 4/45 sera samples (8.9%) using an ELISA test. The presence of BVDV antigen using ELISA was found using ELISA in 3/45 serum samples (6.6%) and 18/172 vaccine samples (10.5%). An RT-PCR was conducted using primers targeting two genome regions, the five prime untranslated region (5’UTR) and N-terminal protease (Npro). BVDV RNA was detected in 33/45 (73.3%) of sera, and 11/172 samples (6.4%) of collected vaccines, of which one vaccine did not declare BVDV strain in its composition. A single serum showed the presence of an infectious virus and only one was contaminated with all 3 species of BVDV. The most frequent species in sera was BVDV-3 (75.5%), whereas in vaccines only BVDV-1 was identified. Sequence analysis showed that the tested commercial sera and one vaccine were contaminated by six genotypes of BVDV: -1a, -1b, -1c, -1d, -2a, and -3. Identification of BVDV and its genetic material in animal-derived products is important due to the possibility of pestivirus transmission as well as the chance of falsifying the results of a diagnostic test. It also demonstrates the necessity of rigorous monitoring of the bioproducts used in the laboratory and industry level.

INVESTIGATION ON BOVINE VIRAL DIARRHEA VIRUS AND INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS IN MONGOLIAN DAIRY FARMS

2017 ◽  
Vol 19 (3) ◽  
pp. 10-15
Author(s):  
Dulam Purevtseren ◽  
Erdenechimeg Dashzevge ◽  
Zhou Wei Guan Guan
Keyword(s):  
Dairy Cows ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Infectious Bovine Rhinotracheitis ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
One Step ◽  
Viral Diarrhea Virus ◽  
Infectious Bovine Rhinotracheitis Virus

In this study, 168 blood sera were collected from dairy cows in Selenge and Tuv aimags during 2013 - 2014. The ELISA was carried out for serological detection of antibodies and antigens to Bovine viral diarrhea virus (BVDV), antibodies to Infectious bovine rhinotracheitis virus (IBRV) in dairy cows in Mongolia. The ELISA results of antibodies of BVDV and antigen of BVDV showed that 86.9% and 3.57%, respectively. The seroprevalence of antibodies against IBRV was found to be 60.7%. In order to confirm of BVDV, One Step RT-PCR was performed in ELISA positive cattle serum samples using specific primer for BVDV. The results showed that 294 bp fragment was successfully amplified. Phylogenetic analysis revealed that the nucleotide sequences 5'UTR gene of the isolates belonged to the BVDV1 subtype. Four isolated virus samples were closely related to China, the another isolate was closely related to the Slovenia BVDV1 isolate.

scholarly journals The effect of bovine viral diarrhea virus introduction on milk production of Dutch dairy herds

2020 ◽  
Author(s):  
Xiaomei Yue ◽  
Wilma Steeneveld ◽  
Mariska van der Voort ◽  
Gerdien van Schaik ◽  
Johannes C.M. Vernooij ◽  
...  
Keyword(s):  
Milk Production ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Dairy Herds ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus
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