Effect of milking behavior on circulatory hormones and milk production in lactating Murrah buffaloes

2016 ◽  
Vol 50 (1) ◽  
Author(s):  
Mahendra Singh ◽  
Anjali Aggarwal ◽  
S. Mallick

The effect of milking temperament on plasma hormones, metabolites, milk yield and composition in lactating Murrah buffaloes was studied. Animals were selected from the institute herd and grouped as docile and nervous buffaloes, based on temperament score before and during milking. Blood sampling times were; before milking (-20 min.), after concentrate intake, after teat massage, during milking and 20 min. post- milking. Plasma prolactin (PRL), growth hormone (GH) and cortisol (CORT) concentrations were measured by enzyme-immunoassay procedures. Milk yields of individual buffaloes were recorded and milk composition (fat, protein, lactose, SNF, SCC) determined. Milk yield was significantly lower (P<0.05) in nervous buffaloes in comparison to docile buffaloes. Mean fat, protein, lactose and SNF varied non-significantly between the groups. Milk SCC and plasma NEFA concentration was more (P<0.05) in nervous buffaloes than in docile buffaloes. Milking temperament significantly influenced plasma PRL (P<0.01) and CORT release (P<0.05) but GH was unaffected. Concentrate feeding prior to milking induced release of plasma PRL and CORT without affecting GH and oxytocin levels. Teat stimulus further enhanced release of PRL, oxytocin and CORT hormone in both the groups. Plasma PRL, cortisol, oxytocin and GH varied significantly (P<0.01) before, during and after milking (P<0.01). Basal PRL, GH and CORT were attained 20 minutes after the completion of milking in both the groups. The results show that the behavior of buffaloes at milking influenced release of galactopoietic hormones (PRL, CORT, GH and oxytocin), milk yield and SCC, while milk fat, protein, lactose and SNF remain unaffected.

Author(s):  
A Nagarjuna Reddy ◽  
Ch Venkata Seshiah ◽  
K Sudhakar ◽  
D Srinivasa Kumar ◽  
P. Ravi Kanth Reddy

The objective of the present study was to determine the effect of the duration of the dry period (DP) on milk yield, milk composition, and reproductive performance of Murrah buffaloes in the subsequent lactation. High yielding Murrah buffaloes (n=48) were assigned to either shortened (30 to 45 d (n=16); and 45 to 60 d (n=16)) or traditional (>60 days (n=16)) dry period lengths. The buffaloes were fed individually according to the production status. The buffaloes in >60, 45 and 30 d dry period groups had similar (P>0.05) milk yield, 6% Fat corrected milk (FCM), milk fat, and total solids; however, the former groups had an increased (P less then 0.05) 6% FCM yield change, and SNF percentage of milk. The mean service period (days) was least (P less then0.05) with the higher number of services required for conception in buffaloes allocated to traditional dry period length compared to those assigned to shortened dry periods. Further, 6% FCM had a negative correlation with Serum Glucose (SG) values at 30 d postpartum, followed by a significant positive (P less then 0.01) correlation at 60 or 90 d postpartum. It is concluded that extended DP of more than 60 days is not advantageous and would be a costly affair for the farmers in both productive and reproductive backdrop.


1984 ◽  
Vol 64 (1) ◽  
pp. 81-91 ◽  
Author(s):  
G. R. VICENTE ◽  
J. A. SHELFORD ◽  
R. G. PETERSON ◽  
C. R. KRISHNAMURTI

Fifteen Holsteins and 12 Ayrshires were randomly assigned within breed and lactation category to one of three isonitrogenous concentrates containing 0% protected tallow (C), 8% soybean-meal-protected-tallow (SPT), or 8% canola-meal-protected-tallow (CPT) which they received during the first 15 wk of lactation. Concentrates and alfalfa hay cubes were offered ad libitum in the ratio 70:30. Statistical analyses compared the effects of diets containing protected tallow (PT) versus the control and secondly the effects of the SPT diet versus the CPT diet. When compared to the control, protected tallow diets significantly increased (P < 0.05) the yields of milk and milk fat and the gross efficiencies of conversion of energy and protein. Plasma NEFA concentration also was significantly higher with PT diets (P < 0.05) than in the control. PT diets significantly reduced milk protein percentage (P < 0.05). There were no significant differences between the SPT and CPT diets for any parameters measured except apparent digestibility of ether extract which was significantly higher for the canola-meal-protected-tallow diet (P < 0.01). Key words: Dairy, cow, milk, composition, protected, lipid, canola


2016 ◽  
Vol 19 (1-2) ◽  
pp. 50-65
Author(s):  
MA Baset ◽  
KS Huque ◽  
NR Sarker ◽  
MM Hossain ◽  
MN Islam

A total of 160 cows, 10 cows in each of native (local cow) and crossbred (local × Holstein Friesian) origins differing in lactation were used in 2×2×2×2 factorial experiment using Randomized Block Design (RBD) to evaluate milk yield and composition of cows considering regions (good & poor feed base region), seasons (dry: Nov.–Feb. 2009 & wet: Jun.–Oct. 2009), genotypes and lactation. A “good and/or poor feed base” region was classified based on the availability of quantity and quality roughages throughout the year. The study revealed that the daily milk yield and 4% FCM of cows under good feed base condition were 6.76 and 6.49 kg, respectively and under poor feed base condition were 3.67 and 3.31 kg, respectively. Feed base region did not affect on milk fat and it was observed that the milk protein, lactose, solids-not-fat (SNF), minerals and total solids under good feed base condition were 37.9, 54.9, 100.9, 6.3 and 140.6 g/kg, respectively, whereas, under poor feed base condition the values were 36.3, 52.9, 98.0, 6.1 and 135.2 g/kg, respectively. Season did not affect milk yield and composition except minerals (6.5 g/kg vs. 5.9 g/kg). Genotypes significantly (p?0.01) influenced daily milk yield, the milk protein and minerals. Lactation did not affect milk yield and the milk protein, but influenced the fat, lactose, SNF, minerals and total solids. The interaction of feed base regions and seasons significantly (p?0.01) influenced milk yield and the milk fat and SNF. The milk protein and lactose was influenced by the interaction of feed bases region, seasons and lactation. Milk yield negatively correlated with fat per cent. The percentage of fat significantly (p?0.01) correlated with protein, lactose, SNF, and minerals %. The percentage protein correlated with lactose, SNF and minerals. Lactose % significantly (p?0.01) correlated with SNF%. It may be concluded that milk yield and composition depends on feed base region, genotype and lactation of cows. Season did not influence milk yield and the composition. Milk yield negatively correlated with the percentage of fat, protein, lactose, SNF and milk composition strongly correlated with each other.Bangladesh J. of Livestock Res. 19(1-2): 50-65, Jan-Dec 2012


1997 ◽  
Vol 77 (1) ◽  
pp. 133-140 ◽  
Author(s):  
A. F. Mustafa ◽  
D. A. Christensen ◽  
J. J. McKinnon

Two studies were undertaken to evaluate the nutritive value of high fiber canola meal (HFCM) relative to regular canola meal for ruminants. In the first study, 21 growing lambs were utilized in a randomized complete block design to determine the total tract digestion coefficients of dry matter (DMD), crude protein (CPD), gross energy (GED), neutral (NDFD) and acid (ADFD) detergent fiber and the digestible energy (DE) contents of seven dehydrated alfalfa-based diets containing 0, 250, 500 and 750 g kg−1 HFCM or canola meal. In the second study, the effect of feeding HFCM as a protein supplement to dairy cows relative to canola meal and soybean meal (SBM) was examined in a triple 3 × 3 Latin square design. Results of the first study showed that HFCM had lower (P < 0.05) DMD, CPD, GED and DE contents than canola meal. The estimated DMD, CPD and GED and DE contents for HFCM were 674 and 795 g kg−1, 695 kJ MJ−1 and 13.68 MJ kg−1, respectively. The corresponding values for canola meal were 707 and 841 g kg−1, 727 kJ MJ−1 and 14.10 MJ kg−1, respectively. Results of the second study showed that protein supplement source had no effect on DM intake, milk yield, milk fat g kg−1, lactose g kg−1 and total solid g kg−1. However, cows fed HFCM- and canola meal-based diets produced milk with lower (P < 0.05) protein content than those fed the SBM-based diet. It was concluded that HFCM had lower total tract nutrient digestion coefficients and DE content compared with canola meal. Incorporation of HFCM in dairy rations up to 100 g kg−1 of the ration had no adverse effect on milk yield or milk composition compared with canola meal. Key words: High fiber, canola meal, total tract digestibility, lactation


2020 ◽  
Vol 87 (3) ◽  
pp. 334-340
Author(s):  
Elisa Manzocchi ◽  
Werner Hengartner ◽  
Michael Kreuzer ◽  
Katrin Giller

AbstractThis research paper addresses the hypotheses (1) that milk produced from hay-fed cows differs from that of silage-fed cows and (2) that silage type has an important impact, too. Four diets differing in forage type but with equal estimated milk production potential and a forage:concentrate ratio of 0.85 : 0.15 were compared regarding their effect on feed intake, milk yield and milk properties. The forages tested were hay, grass silage, conventional short-chopped and long-chopped maize silage subjected to a novel processing technology (Shredlage®). Twenty-four dairy cows were fed two of the four diets in two consecutive runs in an incomplete (4 × 2) Latin-square design (n = 12 per diet). Each experimental period lasted 22 d, with 12 d of adaptation and 10 d of sampling. During sampling, feed intake and milk yield were recorded daily, milk composition and coagulation properties were determined four times. The composition of the diet ingredients was analysed weekly. Data were analysed with a mixed model considering feed, period and their interaction as fixed effects. Stage of lactation, milk yield and milk composition from the pre-experimental period were used as covariates in the model. Dry matter intake was lower with the long-chopped processed maize silage compared to the other three groups. There were some diet differences in intakes of net energy for lactation and absorbable protein in the duodenum, but this did not result in changes in milk yield. The milk fat content was higher with the grassland-based diets compared to the maize silage diets. No treatment effect on milk acidity and rennet coagulation properties was observed. In conclusion, there were no indications for specific physico-chemical properties of milk from a hay-based diet, and maize processing technology was not of large effect either. Future investigations should focus on sensory differentiation of the milk produced with different forages.


Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1379
Author(s):  
Robert Kupczyński ◽  
Antoni Szumny ◽  
Katarzyna Wujcikowska ◽  
Natalia Pachura

The aim of this paper is to review and systematize the current state of knowledge on glycol metabolism in cattle. Glycerol, derived from biodiesel production, must be purified in order to be a useful product for feeding livestock. The use of glycerol in the feeding of ruminants can be justified for several reasons: (i) it is a source of energy in the ration, (ii) it is a glucogenic precursor, and (iii) it may have an effect on milk composition. The high energy value of glycerol provides the opportunity to use this raw material as a partial grain substitute in cattle feed rations. Dietary supplementation of glycerol is associated with increased propionate, butyrate, valerate, and isovalerate concentrations in the rumen. Glycerol can be used at up to 10%–15% of the dietary dry matter (DM) and is well-established as a treatment for ketosis in cows. Glycerol increases plasma glucose and may reduce non-esterified fatty acids and β-hydroxybutyrate levels. The use of glycerol does not have a clear effect on DM intake, milk yield, or milk composition. However, some authors have reported an increase in milk yield after glycerol supplementation associated with decreased milk fat concentration. It is also possible that the concentration in the milk of odd-chain fatty acids and cis-9, trans-11 conjugated linoleic acid may increase after glycerol application.


2003 ◽  
Vol 2003 ◽  
pp. 107-107
Author(s):  
M. H. Fathi ◽  
A. Nikkhah

Cereal grains can provide the major source of energy in diets in order to meet the nutrient requirements of high producing dairy cows. However the amount of starch that can be included in the diets of dairy cows is limited particularly if starch is rapidly fermented such as barley starch. Reduction of feed intake, rumen pH, milk fat test, microbial growth and other metabolic disorders are expected if ruminally degradable starch is fed in amount that cant be efficiently metabolized by rumen microbs. Various techniques for processing barley grain have been developed to decrease the degradability of dry matter in rumen without reducing its extent of digestion. McNiven (1995) showed roasting of barley is more effective treatment. The objective of this experiment was to study of effects the roasting and ammoniation of barley grain on rumen pH, feces pH, milk yield and milk composition in dairy cows.


1985 ◽  
Vol 105 (2) ◽  
pp. 189-196 ◽  
Author(s):  
I. C. Hart ◽  
P. M. E. Chadwick ◽  
S. James ◽  
A. D. Simmonds

ABSTRACT Although it is well known that exogenous bovine GH (bGH) increases milk yield in ruminants it has not been possible to determine whether an increase in endogenous GH secretion has the same effect. The recent isolation of human pancreatic GH-releasing factor (hpGRF-44) has enabled this comparison of the effects of bGH and hpGRF-44 on milk production in sheep. Three pairs of Dorset ewes underwent three 4-day treatments according to a Latin square design. Treatment 1 involved: 2-hourly i.v. injections (∼ 3·0 ml) of bGH (15 μg/kg; 1·8 units/mg); treatment 2: 2-hourly i.v. injections (∼3·0 ml) of hpGRF-44 (0·6 μg/kg); treatment 3: 2-hourly i.v. injections (3·0 ml) of the vehicle. Treatment periods were separated by 10 days. Sheep were milked twice daily and the milk was analysed for fat, protein and lactose. Blood samples (5·0 ml) were taken before and at 15, 45, 75 and 100 min after every third injection throughout the 4 days. Plasma was analysed for insulin, glucose, urea and non-esterified fatty acids (NEFA). The changes in plasma GH stimulated by hpGRF-44 were consistent and repeatable throughout the 4 days of treatment. In comparison to the controls, treatment with hpGRF-44 and bGH significantly increased average plasma GH (μg/l) for the 4 days (control, 5·7±0·2 (s.e.m.); hpGRF-44, 12·3±0·4, P<0·001; bGH, 14·5±0·5, P<0·001), and this was accompanied by similar increases in milk yield (hpGRF-44, 26·7±2·6%, P<0·001; bGH, 30·7±2·9%, P<0·001) and the concentration of milk fat (g/kg) (control, 28·6±0·5; hpGRF-44, 33·2±0·5; bGH 34·8±1·5, P<0·01 for both treatments). The post-treatment decline in milk yield was much slower after hpGRF-44 than after bGH injections. Both treatments stimulated significant increases in the average concentrations of plasma insulin, glucose and NEFA, and plasma urea was significantly reduced by treatment with bGH only. It is concluded that raising endogenous GH secretion is equally as effective as exogenous bGH in stimulating milk production in sheep. J. Endocr. (1985) 105, 189–196


2005 ◽  
Vol 72 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Chirathalattu S Thomas ◽  
Rupert M Bruckmaier ◽  
Karin Östensson ◽  
Kerstin Svennersten-Sjaunja

Milking-related release of oxytocin, prolactin, and cortisol was studied following three pre-milking treatments. Six Murrah buffaloes were treated with direct application of milking cluster (O), a 1-min pre-stimulation (M), and combined feeding and pre-stimulation (MF). Machine milk yield, stripping yield and milk composition were recorded. Milk ejection occurred significantly earlier with MF than M and O (P<0·05; 2·50, 5·10 and 6·33 min, respectively). In all treatments, milk ejection occurred with small increases >3–5 ng/l in oxytocin concentration. Increase in oxytocin concentration over a threshold level and milk ejection occurred simultaneously and were closely correlated (r=0·83, P<0·05). There was a positive correlation between total time oxytocin concentration remained elevated over threshold levels and machine yield (r=0·86, P<0·05). For treatment O, milk ejection was inhibited during machine milking, while a marked increase in oxytocin occurred during hand stripping (6 and 16 ng/l, respectively). For treatment M, mean oxytocin concentrations remained unchanged during pre-stimulation but increased during subsequent machine milking and hand stripping (6·38, 18·06 and 12·36 ng/l, respectively). For treatment MF, although there was a 3·6-fold increase during pre-stimulation, oxytocin increased by 10-fold and 3-fold during machine milking and hand stripping, and was significant for machine milking (P<0·05, 17·32, 47·86, 18·13 ng/l, respectively). Milk-ejection-related cortisol release was visible only in treatment MF. For treatments O and M, prolactin concentration increased prior to the increase in oxytocin. The stripping yield was higher, and fat content in the stripping yield significantly lower, for treatment O indicating incomplete milking. Thus buffaloes are easily disturbed even by small changes in milking routines.


2006 ◽  
Vol 57 (4) ◽  
pp. 377 ◽  
Author(s):  
J. E. Morgan ◽  
N. M. Fogarty ◽  
S. Nielsen ◽  
A. R. Gilmour

Milk production and milk composition were studied in 520 primiparous Merino crossbred ewes that were the progeny of 30 sires from several maternal crossing breeds including Border Leicester, Coopworth, Finnsheep and East Friesian. The ewes were born in 3 years (1997, 1998, and 1999), with 3 sires used in every year to provide genetic links. The crossbred ewe lambs were randomly assigned to autumn or spring joining groups and mated to Poll Dorset rams to lamb at 12 or 19 months of age. Ewes were milked on 3 days during their first lactation at approximately 3, 4, and 12 weeks after lambing. On each milking day, each ewe was initially injected with oxytocin, milked out by machine, and then hand-stripped. This procedure was repeated approximately 4 h later, with the milk weight and time recorded to extrapolate to daily milk yield, and composition of the milk was determined. Daily milk yield was analysed using REML mixed models procedures and 3 measures of milk production were predicted: peak milk yield at 21 days of lactation, total milk yield from 21 to 90 days of lactation, and length of lactation (days for daily yield to decline to 600 g/day). Milk composition traits (%fat, %protein, %lactose) and proportion of machine milk yield were analysed with similar models. Sire breed, number of lambs born and suckled, season, and ewe weight gain from mid-pregnancy until lamb weaning were all significant (P < 0.01) for daily yield, which declined from 2.1 kg/day at 21 days to 0.7 kg/day at 90 days of lactation. The Finnsheep-cross ewes had lower peak milk yield (1.84 ± 0.08 v. 2.09–2.19 ± 0.1 kg/day) and lower total yield of lactation (21–90 days) than all the other breed-cross ewes (78 ± 3 v. 92–107 ± 5 kg). The East Friesian-cross ewes had significantly longer lactations (128.8 ± 10.9 days) than the Border Leicester (98.1 ± 4.4 days) and Coopworth (93.7 ± 4.2 days), with the Finnsheep-cross ewes the shortest (80.1 ± 3.1 days). The East Friesian-cross ewes had the greatest and the Finnsheep-cross ewes the least total yield of lactation (107 ± 5 and 78 ± 3 kg, respectively). There were significant differences in peak milk yield for ewes bearing and suckling single lambs, twin-born and single-raised lambs, and twin-born and raised lambs (1.86 ± 0.04, 2.03 ± 0.09, and 2.37 ± 0.07 kg/day, respectively). There was a significant decline in daily milk yield with increasing ewe weight gain from mid-pregnancy to lamb weaning (–18.1 ± 4.2 g/kg). All the components of milk composition changed from the peak of milk production at 3 weeks to the end of lactation. The East Friesian-cross ewes had significantly lower milk %fat (by approx. 1 percentage point) at both 21 days and 90 days of lactation than ewes by all other sire breeds. The 30 sire progeny mean deviations for daily milk yield ranged from –292 to +276 g/day, with considerable ranges between sires within the sire breeds. The implications for lamb production and breeding programs are discussed.


Sign in / Sign up

Export Citation Format

Share Document