Semen quality and fertility of Bama miniature pig (Sus scrofa domestica)

Author(s):  
Shibin Zhang ◽  
Kuonan Guo ◽  
Deying Kong ◽  
Yu Liu ◽  
Haitao Shang ◽  
...  

Semen quality and fertility of Bama miniature pig and the effect of season on these parameters were determined in this study. Results were as follows: the overall mean ejaculate volume, 55.71 ± 15.21 ml; sperm concentration, 0.66 ± 0.22 × 108/ml; sperm motility, 65.39% ± 16.45%; abnormal sperm percentage, 12.16% ± 7.70%; farrowing rate, 76.44%; litter size, 7.74 ± 2.46. It showed that the semen quality and fertility of Bama miniature pigs were lower than the normal level of the larger commercial and local breeds. In terms of the effect of season, we noted that the semen quality and fertility of Bama miniature pigs were not worst in summer. This may indicate that Bama miniature pig is a relative heat-tolerant breed in comparison to larger commercial and local breeds. These findings in this study could provide reference material for improve management and husbandry in practice and for using Bama miniature pigs as animal model especially in male fertility studies.

Author(s):  
Thea Emily Benson ◽  
Anne Gaml-Sørensen ◽  
Andreas Ernst ◽  
Nis Brix ◽  
Karin Sørig Hougaard ◽  
...  

Bisphenol A (BPA) is considered an endocrine disruptor and has been associated with deleterious effects on spermatogenesis and male fertility. Bisphenol F (BPF) and S (BPS) are structurally similar to BPA, but knowledge of their effects on male fertility remains limited. In this cross–sectional study, we investigated the associations between exposure to BPA, BPF, and BPS and semen quality in 556 men 18–20 years of age from the Fetal Programming of Semen Quality (FEPOS) cohort. A urine sample was collected from each participant for determination of BPA, BPF, and BPS concentrations while a semen sample was collected to determine ejaculate volume, sperm concentration, total sperm count, sperm motility, and sperm morphology. Associations between urinary bisphenol levels (continuous and quartile–divided) and semen characteristics were estimated using a negative binomial regression model adjusting for urine creatinine concentration, alcohol intake, smoking status, body mass index (BMI), fever, sexual abstinence time, maternal pre–pregnancy BMI, and first trimester smoking, and highest parental education during first trimester. We found no associations between urinary bisphenol of semen quality in a sample of young men from the general Danish population.


2019 ◽  
Vol 15 (2-1) ◽  
pp. 303-306
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Ma’aruf Bashir Sani

The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal and abnormal sperm, all for semen characteristics. The results showed a significant difference (P ≤ 0.05) between mass motility, progressive motility, sperm concentration and head defects of 4.85 ± 0.27 to 4.37 ± 0.19, 95.13 ± 0.43 to 81.63 ± 1.15%, 4.93 ± 1.84 to 3.40 ± 1.07×109/ml and 2.96 ± 0.17 to 3.44 ± 0.12% for indigenous and Amo breeds of cockerel, respectively. There were no significant differences observed as semen colour, ejaculate volume, semen pH, live / dead normal sperm neck (mid-piece), tail defects and sperm total abnormalities were found to be 2.85 ± 0.07 to 2.00 ± 0.090.21 ± 0.17 to 0.20 ± 0.02 /ml, 88.85 ± 0.58 to 72.70 ± 0.54% /ml, 11.14 ± 0.58 to 27.29 ± 0.54%, 81.00 ± 0.78 to 66.22 ± 0.61%,9.03 ± 0.42 to 13.96 ± 0.47%, 9.70 ±  to 13.00 ± 0.30 and 21.70 ± 0.59 to 30.40 ± 0.53% for the indigenous and Amo breed groups of cockerel, respectively. It was concluded that semen quality characteristics could be differed between genetically improved (Amo strain) and indigenous breed of cockerels.


2019 ◽  
Vol 7 (1) ◽  
pp. 88-95 ◽  
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95


Lab Animal ◽  
2013 ◽  
Vol 42 (3) ◽  
pp. 86-91 ◽  
Author(s):  
Piren Chen ◽  
Jiafa Hou ◽  
Dezhong Ding ◽  
Xiuguo Hua ◽  
Zhibiao Yang ◽  
...  

2018 ◽  
Vol 35 ◽  
pp. 9-22
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi

The present study was undertaken to determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara, Kaski district. Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3- 4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan 2017). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0. Sperm concentration (0.551 ±0.004 to 1.084 ± 0.014x109), post- thaw motility (42.44±0.11 to 51.72±0.03 %), live sperm (45.62 ± 0.13 to 51.23±0.15 %), and abnormal sperm (11.57±0.12 to 19.59±0.24%) were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. On the other hand, volume (3.98±0.09 to 6.72±0.06 ml), initial motility (72.68±0.02 to 78.64±0.15 %) and pre-filling motility (71.34±0.17 to 77.62±0.02 %) did not differ significantly among different age intervals. Significantly, higher value for semen volume (3.98±0.12 to 6.02±0.14 ml), sperm concentration (0.572±0.119 to 1.095±0.015) and post- thaw motility (40.34±0.21 to 48.54±0.06% was obtained on second collection. However, initial motility (70.58±0.06 to 75.64±0.13 %), prefilling motility (68.11±0.12 to 71.62±0.02 %), live sperm (53.27±0.54 to 56.17±0.02 %) and abnormal sperm (12.31±0.12 to 14.3±0.47 %) did not differ significantly between the frequencies of collection. Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentage were higher in 5-7 years old bulls and in second collection of semen.


Author(s):  
Q. H. Wang ◽  
J. Y. Chen ◽  
K. N. Guo ◽  
X. B. Zheng ◽  
Q. Wu ◽  
...  

The Bama miniature pig (Sus scrofa domestica), endemic in China,which is characterized by its small size and specific meat flavor. The evaluation of pork quality is closely related to boar taint. However, there are no published studies of boar taint in Bama miniature pigs. In this study, the correlation between mRNA expression of boar taint-related genes,boar taint substances (androstenone and skatole) and sex hormones (testosterone, estradiol) of Bama miniature pigs at consecutive ages were examined. We found a gradual increase of mRNA (CYP2E1, CYP2A19, CYP2C34, and COUP-TF1) expression in the livers of pigs aged from birth to 6 months of age. The mRNA expression of COUP-TF1 and CYP2C34 peaked at 12 months old. However, the mRNA expression of CYP2E1 and CYP2A19 declined after 6 months age. The mRNA expression of CYP11A1, CYP17A1, StAR, and COUP-TF1 was stable over time in the testicle of Bama miniature pig. However, a substantial increase in their relative expression levels was observed before sexual maturity. Boar age was positively correlated to sex hormone (testosterone, estradiol) and boar taint substances (androstenone, skatole). There is in vivo gene regulation in boars that controls the relationship between boar taint substances and sex hormones. In addition, CYP11A1, CYP17A1, StAR, and sex hormones (testosterone, estradiol) can be used as markers for low boar taint study. Our study elucidated the correlation of boar taint-related genes,boar taint substances and sex hormones. These results have provided reference for applied research using Bama miniature pigs as a model for livestock production.


2020 ◽  
Vol 46 (5) ◽  
Author(s):  
F. U. Udeh ◽  
I. J. Ohagenyi ◽  
N. E. Ikeh ◽  
M. O. Onodugo ◽  
V. C. Udeh ◽  
...  

The study was conducted to evaluate the semen quality characteristics of turkey parthenotes and parent toms. A total of twenty-four turkeys were used for the study. These comprised of twelve parthenotes toms and twelve parent toms. Parthenotes are male turkeys produced through parthenogenesis. The toms were subjected to abdominal massage techniques for semen collection. Semen collection was done twice per week and analyzed for volume, motility, sperm concentration, like sperm, abnormal sperm, normal sperm, dead sperm, total sperm/ejaculates and total motile sperm cells. The results showed that there were significant differences (p<0.05) in semen volume, sperm concentration, normal sperm cell, abnormal sperm and total motile sperm with mean values being 0.35mL, 1.74x109/mL, 97.80%, 2.20%, 1.68x1012 and 0.41ml, 1.50x109/mL, 94.00%, 6.00% and 1.42x1012, respectively for both parthenotes and parent toms. However, semen parameters such as motility, live sperm cell, dead sperm cell and total sperm/ejaculate showed no significant difference (p>0.05) with values of 96.7%, 97.7%, 2.30% and 6.07x109 for parthenotes toms and 93.20%, 98.50%, 1.50% and 6.23x109 for parent toms. It was observed from the results that turkey parthenotes possess good quality semen characteristic and therefore, recommended for use in poultry industry for turkey production.


2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Pacey

Abstract text Since the very early days of the COVID-19 pandemic, concern has been raised about the possibility of damage to the male reproductive system in those men who are infected with SARS-CoV-2. This was first raised by the early observation (January 2020) that the route of infection was via the Angiotensin Converting Enzyme 2 (ACE-2) receptor present on host cells. ACE-2 was first described in the year 2000 and subsequently shown in 2004 to be selectively expressed in the Leydig cells of the adult testis. This opens up the possibility that although COVID-19 is primarily a respiratory infection, it may also infect the male reproductive system. The authors of several review articles have proposed that male fertility may be theoretically impacted by SARS-CoV-2 in a number of ways. This includes alteration of: (i) testicular architecture; (ii) reproductive hormone profiles (LH/FSH); (iii) spermatogenesis as evidenced by changes to ejaculate quality; (iv) sperm function (e.g., DNA damage); (v) sexual/erectile function; or a combination of all five. Clearly each of these individually may impact on the chance of pregnancy or live birth either in natural or medically assisted reproduction. There is also the possibility that SARS-CoV-2 may be transmitted sexually if sufficient numbers of SARS-CoV-2 are found in semen. Reassuringly, of 14 studies published to date, there is little evidence to suggest that SARS-CoV-2 is present in semen and so the possibility of sexual transmission in patient or donor samples can probably be discounted. However, there is currently an incomplete picture of whether semen quality is affected by SARS-CoV-2 infection as studies are often limited by the fact that no pre-infection control samples are available for direct comparison or they are too short to identify any long-term effects. Nevertheless, the few case-controlled studies published which compare semen quality infected with non-infected (control) individuals suggest that there may be a statistically significant alteration in sperm concentration and motility, although it is not clear whether this is linked to infection by the SARS-CoV-2 virus or simply a consequence of febrile illness and fever (or medication given to combat the infection). There is currently a lack of long-term data on any impact of COVID-19 on male reproductive hormones (although much speculation about the role that testosterone might play in the severity of disease). There are also emerging reports of increased risk of erectile dysfunction in men following SARS-CoV-2. In terms of birth rates, it is simply too early to tell whether these have been affected by the pandemic, given the possibility of lockdown affecting sexual behaviour in fertile couples and IVF clinic closures in infertile couples. In conclusion, although the COVID-19 pandemic has infected over 130 million people worldwide we still know too little about the impact of SARS-CoV-2 on the male reproductive system. Given the incidence of long-COVID, and the asymptomatic nature of the infection for some, it is important to commission and conduct long-term studies which can monitor the reproductive outcomes of young men who have survived a SARS-CoV-2 infection.


2021 ◽  
Vol 22 (2) ◽  
pp. 108-112
Author(s):  
U. Herbert ◽  
C. O. Acha

A total of sixteen rabbits sourced from two locations in Southeastern Nigeria were used for this study. Semen was collected from all the rabbits twice weekly for eight weeks using an artificial vagina while observation on the effect of time of collection on semen quality were also made. The results obtained indicated that time of collection had no significant effect (P > 0.05) on all the seminal characteristics measured. However, source of the animals showed significant effect (P < 0.05) on semen volume, sperm concentration, total sperm per ejaculate and proportion of abnormal sperm. Average semen volume, sperm concentration and total sperm per ejaculate were: 0.69±0.007 ml, 104.0±1.44 x 106/m1 and 71.40±0.90x106 respectively for the rabbits sourced from Port Harcourt. The corresponding values for rabbits sourced from Owerri were: 0.66±0.008m1, 96.02 ±1.63x106iml and 63.49±1.50 x 106 respectively. Abnormal sperm proportions were 11.05±0.22% and 1216 ± 032% for the rabbits sourced from Port Harcourt and Owerri respectively. There were no significant (P> 0.05) differences observed between locations in live sperm concentration and sperm motility. Live sperm concentration and sperm motility were 87.75±1.09% and 71.62±0.74% for the Port Harcourt group, while the values obtained for the Owerri group were 88.06±034% and 71.19±0.89% respectively.  


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