Genetic polymorphism in the promoter of prolactin gene in layer chicken

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
T. K. Bhattacharya ◽  
R. N. Chatterjee ◽  
U. Raj Kumar ◽  
K. S. Rajaravindra ◽  
M. Niranjan
Keyword(s):  
Genetic Polymorphism ◽  
White Leghorn ◽  
Promoter Fragment ◽  
White Leghorn Chicken ◽  
Layer Chicken ◽  
Prolactin Gene ◽  
Allelic Distribution ◽  
Layer Control

Genetic polymorphism of promoter (Fragment 1, 2 and 3) of prolactin gene in four strains of White Leghorn chicken layers i.e. IWH, IWI, IWK and layer control was identified. Two alleles (A and B) were found in all the promoter fragments of the gene. In above mentioned layer populations, A allele was predominant. The genotypic and allelic distribution was significantly differed among the populations. Different demographic and divergent parameters were estimated in all the lines. It is concluded that all 3 fragments of promoter of prolactin gene was polymorphic in four strains of White Leghorn layer populations.

scholarly journals PCR-RFLP Analysis of Single Nucleotide Polymorphism (SNP) C-2402T at the Promoter Region of Prolactin Gene and its Association with Positively Correlated Production Traits in White Leghorn Chicken

2021 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T.V. Aravindakshan
Keyword(s):  
Venous Blood ◽  
Rflp Analysis ◽  
Production Traits ◽  
White Leghorn ◽  
Coding Region ◽  
Single Nucleotide ◽  
White Leghorn Chicken ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Flanking Region

Background: The avian prolactin gene is highly conserved, located on chromosome number 2 and most sequence polymorphisms occurs in the 5’ flanking region, 3’ flanking region, and the coding region of signal peptide. The present study was aimed at the identification of SNP C-2402T of prolactin gene and its association with production traits in White Leghorn chicken. Methods: A total of 200 birds of White Leghorn were selected from All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy. Genomic DNA was isolated from venous blood. Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis was done to identify the SNP C-2402T of prolactin gene. Result: All the birds were observed with the same genotype CC and the frequency of the C allele was one.

scholarly journals Quantitative and Qualitative Protein Changes in the Developing Single Comb White Leghorn Chicken Embryo

1972 ◽  
Vol 51 (1) ◽  
pp. 313-320 ◽  
Author(s):  
J.A. Asmar ◽  
P.L. Pellett ◽  
Nur Hariri ◽  
M.D. Hariri
Keyword(s):  
Chicken Embryo ◽  
White Leghorn ◽  
White Leghorn Chicken ◽  
Single Comb

scholarly journals Genotypic confirmation of non-broody behaviour of white leghorn by 24bp indel(s) polymorphism at the promoter region of prolactin gene

2020 ◽  
Vol 8 (2) ◽  
pp. 2491-2493
Author(s):  
Azhaguraja Manoharan ◽  
S Sankaralingam ◽  
P Anitha ◽  
Binoj Chacko ◽  
TV Aravindakshan
Keyword(s):  
Promoter Region ◽  
White Leghorn ◽  
Prolactin Gene

The sclerotic plates of the White Leghorn chicken

1942 ◽  
Vol 84 (3) ◽  
pp. 295-306 ◽  
Author(s):  
Norman M. Nelson
Keyword(s):  
White Leghorn ◽  
White Leghorn Chicken

Genetic Polymorphism in Prolactin Gene and its Effect on Test Day Milk Production Traits in Sahiwal Cattle

2021 ◽  
Author(s):  
T. Karuthadurai ◽  
A.K. Chakravarty ◽  
A. Kumaresan ◽  
D.N. Das ◽  
A. Sakthivel Selvan ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Milk Production ◽  
Milk Yield ◽  
Early Stage ◽  
Direct Sequencing ◽  
Production Performance ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Sahiwal Cattle ◽  
Fat Yield

Background: The selection of genetically superior animals at an early stage of life, the molecular markers are used along with traditional selection. The study was carried out to identify the genetic polymorphism in the exon3 region of the Prolactin and enumerate its effect on milk production performance in Sahiwal cattle. Prolactin plays an imperative regulatory role in mammary gland development, milk emission and lactogenesis. Analysed the sequence of this gene to explore whether mutations in this sequence and it could be accountable for quantitative variations in milk production and its composition traits.Methods: Total DNA was isolated from the blood samples of 98 pedigreed Sahiwal population. Using PCR-RFLP method and direct sequencing, noticed a single-nucleotide polymorphism in exon3 region of the Prolactin gene in 156bp and also the effect of non- genetic factors on each trait was assessed by least-squares analysis for non-orthogonal data by a fixed model.Result: PCR-RFLP was done with RsaI restriction endonuclease for the identification of different genotypes. The frequency of G and A alleles of the Prolactin gene was evaluated as 0.575 and 0.425, whereas the frequencies of GG, GA and AA genotypes for the Prolactin gene were 0.45, 0.25 and 0.30, respectively. SNP (G55A) conferred an increase in test-day milk yield around 321.5g, in test day fat yield around 13.9g and in test day SNF yield increase was 19.4g, respectively. High correlation was perceived from test day (TD2) onwards between test day traits and lactation milk yield indicating that selection based on identified SNP in TD2 increased test day milk yield, fat yield and SNF yield by 1.1472 kg, 29.6gm and 45.4gm, respectively.

Genetic studies on egg production traits in IWN and IWP strains of White Leghorn chicken

2018 ◽  
Vol 53 (3) ◽  
pp. 247
Author(s):  
Snehal D. Patil ◽  
F.P. Savaliya ◽  
A.B. Patel ◽  
H.I. Paleja ◽  
R.S. Joshi ◽  
...  
Keyword(s):  
Egg Production ◽  
Production Traits ◽  
White Leghorn ◽  
Genetic Studies ◽  
White Leghorn Chicken

Modulation of arginine vasotocin secretion in chicks by dehydration during embryogenesis

1994 ◽  
Vol 142 (1) ◽  
pp. 153-160 ◽  
Author(s):  
S M Kisliuk ◽  
R Grossmann
Keyword(s):  
Relative Humidity ◽  
Environmental Temperature ◽  
Arginine Vasotocin ◽  
Plasma Sodium ◽  
White Leghorn ◽  
Control Groups ◽  
Additional Increase ◽  
Male And Female ◽  
White Leghorn Chicken ◽  
Low Humidity

Abstract The influence of low humidity during incubation (LHI) on arginine vasotocin (AVT) secretion in male and female White Leghorn chicken embryos and chicks was investigated. LHI embryos were incubated from day 8 (E8) to E18 at a relative humidity of 32–35%, while control embryos were incubated at 58–62%. In the control groups, plasma AVT gradually increased from E15 to E18, increased sharply during hatching and returned to the El8 level within 4 h. LHI did not alter the level of AVT until E18. LHI E19 males had concentrations of AVT twice as high as those of control males (39 ± 3·9 vs 15 ± 1·6 pmol/l, P<0·001). Augmentation persisted during hatching and 4 h afterwards. In LHI females additional elevation reached significant levels only at 4 h after hatching (58 ± 13 vs 16 ± 1·7 pmol/l, P<0·01). Deprivation of water caused an increase in plasma sodium concentrations in the 2-day-old chicks of the control groups, while no significant changes were observed in the LHI group. Deprivation of water together with a high environmental temperature caused an additional increase in AVT only in the LHI groups (42 ± 5·2 vs 22 ± 3·9 pmol/l, P<0·01 for males; 32 ± 3·4 vs 19 ± 2·8 pmol/l, P<0·01 for females). The results clearly showed that LHI leads to a more pronounced response of the AVT system on subsequent osmotic and non-osmotic challenges, especially in males. Embryonal activation of the AVT system probably helps the organism to cope with hatching and posthatching stresses. Journal of Endocrinology (1994) 142, 153–160

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