Characterization and antibiogram of enteropathogenic Escherichia coli isolated from diarrhoeic and non-diarrhoeic dogs in South Bengal, India

2015 ◽  
Author(s):  
A. Banik ◽  
D. P. Isore ◽  
S. N. Joardar ◽  
K. Batabyal ◽  
S. Dey
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
West Bengal ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Enteropathogenic Escherichia Coli ◽  
Highly Pathogenic ◽  
E Coli ◽  
Sheep Blood ◽  
Common Serotypes

Diarrhoea in canines is mainly caused by Escherichia coli which can be fatal also. To understand the depth of this infection, a study was undertaken to detect E. coli isolates from diarrhoeic and non-diarrhoeic dogs in Southern part of West Bengal. A total of 112 canine samples were tested during May to September 2012 revealing approx 63.4% (71) samples positive for Escherichia coli. The most common serotypes were O8 (23.9%) followed by O157 (19.7%), O101 (16.9%), O26 (15.5%), O153 (12.7%) and O6 (11.3%). Among these O6, O8, O26 and O157 were highly pathogenic to mice causing almost 100% mortality within 24hrs of inoculation and were also detected to be haemolytic on sheep blood agar plates except serotype O8. These isolates were mostly sensitive to nalidixic acid (80.29%), cotrimoxazole (78.88%), ciprofloxacin (74.65%), colistin and ceftriaxone (both 71.83%) but were resistant to amikacin (97.18%), kanamycin (95.78%), cephalexin (92.96%) and enrofloxacin (84.51%).

scholarly journals Mechanistic insights into synergy between nalidixic acid and tetracycline against clinical isolates of Acinetobacter baumannii and Escherichia coli

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Amit Gaurav ◽  
Varsha Gupta ◽  
Sandeep K. Shrivastava ◽  
Ranjana Pathania
Keyword(s):  
Escherichia Coli ◽  
Acinetobacter Baumannii ◽  
Nalidixic Acid ◽  
Bacterial Infections ◽  
Clinical Isolates ◽  
Hospital Environment ◽  
Infection Model ◽  
Drug Resistant ◽  
E Coli

AbstractThe increasing prevalence of antimicrobial resistance has become a global health problem. Acinetobacter baumannii is an important nosocomial pathogen due to its capacity to persist in the hospital environment. It has a high mortality rate and few treatment options. Antibiotic combinations can help to fight multi-drug resistant (MDR) bacterial infections, but they are rarely used in the clinics and mostly unexplored. The interaction between bacteriostatic and bactericidal antibiotics are mostly reported as antagonism based on the results obtained in the susceptible model laboratory strain Escherichia coli. However, in the present study, we report a synergistic interaction between nalidixic acid and tetracycline against clinical multi-drug resistant A. baumannii and E. coli. Here we provide mechanistic insight into this dichotomy. The synergistic combination was studied by checkerboard assay and time-kill curve analysis. We also elucidate the mechanism behind this synergy using several techniques such as fluorescence spectroscopy, flow cytometry, fluorescence microscopy, morphometric analysis, and real-time polymerase chain reaction. Nalidixic acid and tetracycline combination displayed synergy against most of the MDR clinical isolates of A. baumannii and E. coli but not against susceptible isolates. Finally, we demonstrate that this combination is also effective in vivo in an A. baumannii/Caenorhabditis elegans infection model (p < 0.001)

Spheroplasts of enteropathogenic Escherichia coli. I. Biological characteristics

1968 ◽  
Vol 14 (6) ◽  
pp. 675-678 ◽  
Author(s):  
B. Diena ◽  
R. Wallace ◽  
L. Greenberg
Keyword(s):  
Escherichia Coli ◽  
Tissue Culture ◽  
Biological Characteristics ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
O Antigen ◽  
Pathogenic Serotypes

The properties of glycine-induced spheroplasts of six pathogenic serotypes of E. coli were investigated. Fimbriae and flagella appeared to be only partially synthesized as was the somatic O antigen. Cytopathogenicity of these spheroplasts for tissue culture was reduced and the infection of the monolayers was retarded as compared with the normal bacillary forms. Sensitivity to phage was almost completely lost, suggesting that glycine had either interfered with the synthesis of phage receptors or had altered the mucopeptide layerwhich is the substrate for phage enzymes. Alternatively, the phage may become a prophage inside the spheroplast with the loss of virulence.

Comparison of colony and stool blots for detection of enteropathogens by DNA probes

1991 ◽  
Vol 37 (5) ◽  
pp. 407-410
Author(s):  
Mônica A. M. Vieira ◽  
Beatriz E. C. Guth ◽  
Tânia A. T. Gomes
Keyword(s):  
Escherichia Coli ◽  
Key Words ◽  
Sensitivity And Specificity ◽  
Dna Probes ◽  
Type I ◽  
Enteropathogenic Escherichia Coli ◽  
Key Words Dna ◽  
E Coli ◽  
Heat Stable

DNA probes that identify genes coding for heat-labile type I (LT-I) and heat-stable type 1 (ST-I) enterotoxins, enteropathogenic Escherichia coli adherence factor (EAF), and Shigella-like, invasiveness (INV) are used to evaluate the sensitivity and specificity of stool blots in comparison with the sensitivity and specificity of colony blots in detecting enteropathoghens. The sensitivities of the probes in stool blots are 91.7% for the LT-I probe, 76.9% for the ST-I probes, 78.9% for the EAF probe, and 45.5% for the INV probe. The specificity of all probes is higher than 95%. In general, the stool blot method identifies as many if not more LT-I-, ST-I-, and EAF-producing E. coli infections than the colony blots. Key words: DNA probes, stool blots, enteropathogens, diagnosis.

Rapid Streptococcal Tests

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 288-288
Author(s):  
S. DUBOSE RAVENEL ◽  
GREGORY CARL ELLIS ◽  
WILLIAM N. MICHAL
Keyword(s):  
Blood Culture ◽  
Sensitivity And Specificity ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Practice Setting ◽  
Throat Culture ◽  
Culture Techniques ◽  
Sheep Blood ◽  
Office Practice ◽  
Important Study

Roddey et al have reported an important study on the sensitivity and specificity of the Strep A OIA test compared with two culture techniques—5% sheep blood agar and Todd-Hewitt broth—in an office practice setting. They found the sensitivity and specificity of OIA as compared with sheep blood culture to be 91.4% and 95.6%, and compared with the broth method, 90.4% and 94.1%, respectively. They conclude that the OIA method is preferable for the majority of their patients, but recommend a throat culture be performed in cases with a negative OIA test.

scholarly journals Novel Hybrid of Typical Enteropathogenic Escherichia coli and Shiga-Toxin-Producing E. coli (tEPEC/STEC) Emerging From Pet Birds

2018 ◽  
Vol 9 ◽  
Author(s):  
Rosely Martins Gioia-Di Chiacchio ◽  
Marcos Paulo Vieira Cunha ◽  
Lilian Rose Marques de Sá ◽  
Yamê Minieiro Davies ◽  
Camila Bueno Pacheco Pereira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Pet Birds

scholarly journals Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin

2015 ◽  
Vol 45 (7) ◽  
pp. 1249-1255 ◽  
Author(s):  
Daiane Carvalho ◽  
Fabrine Finkler ◽  
Tiela Trapp Grassotti ◽  
Hiran Castagnino Kunert Filho ◽  
Francisco Esmaile de Sales Lima ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Pathogenic Potential ◽  
Highly Pathogenic ◽  
E Coli ◽  
Intermediate Degree

The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (&gt;75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates were classified as highly pathogenic (2.7%), intermediate (10.1%), low (42.2%) and apathogenic (45%). It was observed a wide variation in the susceptibility profile of isolates in relation to antimicrobials. It was also found that most of the samples had pathogenic potential (55%), thus being considered as APEC (avian pathogenic E. coli). No relationship between pathogenicity and antimicrobial susceptibility (P≤0.05) was observed.

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