Molecular characterization and polymorphism detection in HSPB6 gene in Sahiwal cattle

2015 ◽  
Author(s):  
Rakesh Kumar ◽  
I. D. Gupta ◽  
Archana Verma ◽  
Nishant Verma ◽  
Ankit Magotra ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Marker Assisted Selection ◽  
Genetic Improvement ◽  
Bos Taurus ◽  
Comparative Sequence Analysis ◽  
Allelic Variants ◽  
Exon 2 ◽  
Polymorphism Detection ◽  
Sahiwal Cattle ◽  
Amplicon Size

The present study was undertaken with the objectives of molecular characterization and detection of genetic polymorphisms in the HSPB6 gene of Sahiwal cattle. A total of 100 Sahiwal cattle maintained at LRC, NDRI, Karnal (Haryana) were included in the study. HSPB6 gene has been mapped on Bos taurus autosome 18 (BTA 18) and spans nearly 2620 bp comprising 3 exons and 2 introns. Four sets of forward and reverse gene-specific oligonucleotide primers of HSPB6 gene were designed using Primer3 software and amplicon size of 529 bp, 460 bp, 670 bp and 502 bp were ascertained. On the basis of comparative sequence analysis, total three polymorphic loci (SNPs) were detected at locus G436A, A2152G and A2417T in HSPB6 gene as compare to Bos taurus (NCBI GenBank AC_000175.1). Out of these three SNPs, two (A2152G & A2417T) were found in 3′-UTR region and third one (G436A) was detected in intronic region 1. However, monomorphic pattern was found in exon 2 of HSPB6 gene in Sahiwal cattle. Further research need be directed to find out the association of allelic variants in major heat shock protein genes to establish markers for the future genetic improvement of livestock through marker assisted selection.

Novel combined insulin-like 3 variations of a single nucleotide in cryptorchidism

2019 ◽  
Vol 32 (9) ◽  
pp. 987-994 ◽  
Author(s):  
Xenophon Sinopidis ◽  
Roza Mourelatou ◽  
Eirini Kostopoulou ◽  
Alexia Karvela ◽  
Andrea-Paola Rojas-Gil ◽  
...  
Keyword(s):  
Phenotypic Variability ◽  
Testicular Descent ◽  
Sequencing Analysis ◽  
Single Nucleotide ◽  
Allelic Variants ◽  
Exon 2 ◽  
Pcr Products ◽  
Exon 1 ◽  
Male Patients ◽  
Allelic Variations

Abstract Background Insulin-like 3 hormone (INSL3) is involved in the process of testicular descent, and has been thoroughly studied in cryptorchidism. However, INSL3 allelic variations found in the human genome were heterozygous and only a few of them were found exclusively in patients with cryptorchidism. Under this perspective, we aimed to study the presence of INSL3 allelic variations in a cohort of patients with cryptorchidism and to estimate their potential consequences. Methods Blood samples were collected from 46 male patients with non-syndromic cryptorchidism and from 43 age-matched controls. DNA extraction and polymerase chain reaction (PCR) were performed for exons 1 and 2 of the INSL3 gene in all subjects. Sequencing analysis was carried out on the PCR products. All data were grouped according to testicular location. Results Seven variations of a single nucleotide (SNVs) were identified both in patients with cryptorchidism and in controls: rs2286663 (c.27G > A), rs1047233 (c.126A > G) and rs6523 (c.178A > G) at exon 1, rs74531687 (c.191-30C > T) at the intron, rs121912556 (c.305G > A) at exon 2 and rs17750642 (c.*101C > A) and rs1003887 (c.*263G > A) at the untranslated region (UTR). The allelic variants rs74531687 and rs121912556 were found for the first time in the Greek population. The novel homozygotic combination of the three allelic variants rs1047233-rs6523-rs1003887 seemed to present a stronger correlation with more severe forms of cryptorchidism. Conclusions The combination of specific INSL3 SNVs rather than the existence of each one of them alone may offer a new insight into the involvement of allelic variants in phenotypic variability and severity.

Molecular characterization and SNP identification in HSPB1 gene in Murrah buffalo

2015 ◽  
Author(s):  
Ashwani Arya ◽  
Archana Verma ◽  
I. D. Gupta ◽  
Shahid A. Shergojry ◽  
Ankit Magotra ◽  
...  
Keyword(s):  
Heat Shock ◽  
Marker Assisted Selection ◽  
Bos Taurus ◽  
Nucleotide Position ◽  
Coding Region ◽  
Murrah Buffalo ◽  
Future Improvement ◽  
Pcr Products ◽  
Exon 1 ◽  
Heat Shock Protein Genes

Present study was conducted on 100 lactating Murrah buffaloes maintained at LRC, NDRI Karnal (Haryana) to characterize and to identify genetic polymorphisms in HSPB1 gene. Two sets of primers specific to coding sequence of HSPB1 gene were designed using Primer3 software and PCR products of 631 bp and 670 bp were obtained. Amplicons were custom sequenced and subjected to ClustalW analysis which revealed 8 nucleotide changes, 7 in non coding region and one in coding region in Murrah buffalo sequence as compared to Bos taurus. Only one SNP at nucleotide position G225A in exon 1 of HSPB1 gene was observed in Murrah buffalo, which resulted in two genotypes GG and AG with respective frequency of 0.84 and 0.16 indicating the existence of variability in the sampled population. The study has opened the possibility of identifying and using genetic variations in major heat shock protein genes, for the future improvement of livestock through marker assisted selection.

Phenotypic and genetic parameters for milk yield in traditional Nublang cattle (Bos indicus) of Bhutan

2015 ◽  
Vol 57 ◽  
pp. 25-30
Author(s):  
Jigme Dorji ◽  
Dhendup ◽  
Iona M. MacLeod
Keyword(s):  
Milk Yield ◽  
Genetic Improvement ◽  
Genetic Parameters ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Sustainable Utilization ◽  
Dual Purpose ◽  
Lactation Length ◽  
Daily Milk Yield ◽  
Daily Milk

SummaryThe Nublang is a unique dual-purpose breed ofBos indicuscattle found in Bhutan. They have been crossed with Mithun (Bos frontalis) andBos taurusbreeds in an effort to improve milk production leading to a decline in the population of purebred Nublang. An alternative to crossbreeding would be the genetic improvement of milk yield within purebred Nublang, thus allowing conservation and sustainable utilization of the pure breed. It is important to measure the genetic variability (heritability) of milk yield to assess the potential for genetic improvement within Nublang. Therefore this study estimated phenotypic and genetic parameters of milk yield using 2 052 test day milk yields from 66 cows recorded from 1997 to 2013 in the National Nublang Farm, Tashiyangphu. The average daily milk yield (DMY) was 2.12 ± 0.7 litres (N 2 052, range: 0.3–5.0 L, CV: 34 percent). Parity, month in milk and year all had a significant effect on DMY (P< 0.05). The average lactation milk yield (LMY) was 519.2 ± 151 L (N: 261, range: 115–881.7, CV: 29.1 percent) and average lactation length was 239 days. LMY was significantly influenced by parity (P< 0.05) and season of calving. The heritability of DMY and LMY was 0.22 ± 0.16 and 0.13 ± 0.20, respectively. The repeatability estimates were 0.45 ± 0.05 and 0.49 ± 0.08 for DMY and LMY, respectively. Overall the heritability and repeatability estimates of milk yields indicate potential for genetic improvement of milk yield in Nublang cattle through selection. However, it is recommended that a larger dataset is generated to enable more precise estimates of genetic parameters.

Bos indicus and Bos taurus crossbred dairy cattle in Australia. IV.* Progeny testing and expected rate of genetic improvement

1976 ◽  
Vol 27 (2) ◽  
pp. 309 ◽  
Author(s):  
IR Franklin ◽  
RH Hayman ◽  
RO Hewetson
Keyword(s):  
Dairy Cattle ◽  
Heat Tolerance ◽  
Genetic Improvement ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Progeny Testing ◽  
Dairy Farmers ◽  
Improvement Program ◽  
Tropical Environments ◽  
Tick Resistance

A dairy improvement program designed to develop a breed of cattle adapted to tropical environments is described. Each year young crossbred (Bos indicus x Bos taurus) bulls are screened for heat tolerance and tick resistance, and then progeny-tested in the herds of cooperating dairy farmers. Estimates of phenotypic and genetic means, variances and correlations are presented for production of milk and milk components, and the rate of genetic improvement is discussed. In particular the heritability of milk yield in the crossbred progeny is 0.27, and the theoretical rate of improvement is 2.6% per year. ____________________ *Part III, Aust. J. Agric. Res., 25: 1023 (1974).

Molecular characterization and SNP identification in HSPB6 gene in Karan Fries (Bos taurus x Bos indicus) cattle

2017 ◽  
Vol 49 (5) ◽  
pp. 1059-1063
Author(s):  
Rakesh Kumar ◽  
I. D. Gupta ◽  
Archana Verma ◽  
Ragini Kumari ◽  
Nishant Verma
Keyword(s):  
Molecular Characterization ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Karan Fries

Effect of Sex on the Body Weights of Pure Breed Sahiwal Calf under the High Humid Conditions of Assam

2020 ◽  
Author(s):  
Upasana Baruah ◽  
Purabi Kaushik ◽  
G.C. Das ◽  
D. Kalita ◽  
T.K. Amonge ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Genetic Improvement ◽  
The Body ◽  
Veterinary Science ◽  
Cattle Farm ◽  
North East India ◽  
North East ◽  
Humid Condition ◽  
Body Weights ◽  
Sahiwal Cattle

Background: Sahiwal is an outstanding milch breed of cattle originated from the Sahiwal area, Montgomery district of Punjab in Pakistan. Performance evaluation of this breed in the high humid condition of north-east India is very much limited. Assam Agricultural University is maintaining a pure herd of Sahiwal cattle in the College of Veterinary Science, Khanapara since August 2017 to conserve, propagate and bring about genetic improvement of this breed.Methods: The study was carried out in Sahiwal cattle farm, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati by utilizing 25 numbers of Sahiwal calves. Least squares analysis of variance technique (1975) was undertaken to analyze the body weights of calves at monthly intervals up to six months of age. Result: Overall mean for body weights of calves were found as 26.96 ± 0.27 kg, 32.03 ± 0.47 kg, 37.03 ± 0.62 kg, 42.91 ± 0.76 kg, 48.45 ± 0.77 kg, 55.22 ± 0.77 kg, and 62.08 ± 0.88 kg at the day of birth, 1st month, 2nd month, 3rd month, 4th month, 5th month and 6th month respectively. Analysis of variance revealed that the bodyweight of male calves was significantly heavier than that of the female calves ( less than 0.05).

Molecular characterization of Ovar-DRB1 exon2 gene in Garole sheep resilient to gastrointestinal nematodes

2015 ◽  
Author(s):  
C. Paswan ◽  
L. L.L. Prince ◽  
R. Kumar ◽  
C. P. Swarnkar ◽  
D. Singh ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Molecular Characterization ◽  
Molecular Mechanisms ◽  
Gastrointestinal Nematodes ◽  
Exon 2 ◽  
Pcr Rflp

Ovine MHC DRB1 exon 2 (Ovar-DRB1.2) gene is arguably one of the critical genes, responsible for disease resistance against parasite in animals. This study was carried out for indepth analysis of polymorphism in MHC DRB1.2 gene and to explore the underlying molecular mechanisms for the development of disease resistance in Garole. PCR-RFLP study revealed that the gene is polymorphic in nature. The frequency of allele ‘A’ for endonuclease SacI and Allele ‘B’ for endonuclease Hin1I were significantly higher in Garole population.

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