Genetic characterization of dahlem red layers by PCR-RAPD

2016 ◽  
Author(s):  
Ch. Shivaprasad ◽  
B. Rarmesh Gupta ◽  
R. N. Chatterjee
Keyword(s):  
Genetic Distance ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Genetic Characterization ◽  
Dna Polymorphisms ◽  
Randomly Amplified Polymorphic Dna ◽  
Band Frequency

A total of one hundred Randomly Amplified Polymorphic DNA (RAPD) markers were utilized to detect the DNA polymorphisms in a total of 48 full sib mated, half sib mated and non-in bred groups of Dahlem Red birds. Twenty one per cent of the primers tested yielded distinct polymorphic RAPD profiles. Out of 341 amplified bands 204 bands (59.82 %) were found to be polymorphic. The genetic similarity ranged from 83.94 to 87.90 % and, 81.14 to 85.25 % with in group based on band sharing and frequency respectively. Full sib group showed higher genetic similarity then non-in bred group. The genetic similarity based on band frequency ranged from 88.07 to 90.18 %, based on band frequency varied from 72.78 to 79.53 percent. The full sib and half sib groups showed maximum genetic similarity. The genetic distance between full sib and non-inbred groups was found to be the maximum (0.3176), while it was minimum between full sib and half sib groups.

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

Molecular Diversity Analysis of Lentil (Lens culinaris Medik.) through RAPD Markers

2012 ◽  
Vol 22 (1) ◽  
pp. 51-58 ◽  
Author(s):  
M.E. Hoque ◽  
M.M. Hasan
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Group Method ◽  
Diversity Analysis ◽  
Pair Group ◽  
Molecular Genetic Diversity

Random Amplified Polymorphic DNA (RAPD) markers were used to study the molecular genetic diversity analysis among six BARI released lentil varieties viz. BARI masur-1, BARI masur-2, BARI masur-3, BARI masur-4, BARI masur-5 and BARI masur-6. PCR amplified products were visualized on 1.0% agarose gel and the band for each primer were scored. Ten RAPD markers were used in this study. Out of them 7 primers showed amplification of 53 DNA fragments with 60.37% of them being polymorphic. The highest number of polymorphic loci was noticed in the variety BARI masur-3. The same variety also showed maximum Nei’s gene diversity value (0.0552). The highest Nei’s genetic distance (0.5002) was observed in BARI masur-1 vs. BARI masur-5 whereas, the lowest genetic distance (0.0692) was found in BARI masur-1 vs. BARI masur-2. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei’s genetic distance grouped the six cultivars into two main clusters. BARI masur-1, BARI masur-2 and BARI masur-3 were in cluster I and BARI masur-4, BARI masur-5 and BARI masur-6 were in cluster II. The cultivar BARI masur-4 was closest to the cultivar BARI masur-6 with the lowest genetic distance (0.0972) and the highest genetic distance (0.5002) was found between BARI masur-1 and BARI masur-5. The RAPD markers were found to be useful in molecular characterization of lentil varieties which could be utilized by the breeders for the improvement of lentil cultivars. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11260 Plant Tissue Cult. & Biotech. 22(1): 51-58, 2012 (June)

scholarly journals Genetic Characterization of Cleveland Bay Horse Breed

Diversity ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 174 ◽  
Author(s):  
Khanshour ◽  
Hempsey ◽  
Juras ◽  
Cothran
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Genetic Resource ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Similarity Measures ◽  
Horse Breed ◽  
The Usa

The Cleveland Bay (CB) is the United Kingdom’s oldest established horse breed. In this study we analyzed the genetic variability in CB horses and investigated its genetic relationships with other horse breeds. We examined the genetic variability among 90 CB horses sampled in the USA compared to a total of 3447 horses from 59 other breeds. Analysis of the genetic diversity and population structure was carried out using 15 microsatellite loci. We found that genetic diversity in CB horses was less than that for the majority of other tested breeds. The genetic similarity measures showed no direct relationship between the CB and Thoroughbred but suggested the Turkman horses (likely in the lineage of ancestors of the Thoroughbred) as a possible ancestor. Our findings reveal the genetic uniqueness of the CB breed and indicate its need to be preserved as a genetic resource.

scholarly journals Characterization of DNA polymorphisms in Caryocar brasiliense (Camb.) in populations with and without thorn at the endocarp by RAPD markers

2010 ◽  
Vol 82 (3) ◽  
pp. 779-789 ◽  
Author(s):  
Luciana N. Londe ◽  
Carlos Ueira-Vieira ◽  
Warwick E. Kerr ◽  
Ana Maria Bonetti
Keyword(s):  
Rapd Markers ◽  
Dna Polymorphisms ◽  
Ferric Reductase ◽  
Iron And Steel ◽  
Popular Medicine ◽  
Brazilian Savannah ◽  
Species Analysis ◽  
Its Gene ◽  
Caryocar Brasiliense

Caryocar brasiliense (pequi), is one of the main species at the biome of the Brazilian savannah due to its use in culinary, popular medicine, industry in general, and iron and steel industry. At São José do Xingu (MT), a tree of C. brasiliense without thorn at the endocarp was found, which enables the improvement of C. brasiliense not only for consumption but also to the high appreciation it already has. To detect the existing differences between the pequi with and without the thorn at the endocarp, RADP markers were used. The generated polymorphisms were cloned and sequenced in order to identify the sequences that are responsible for the fenotypical alteration. It was observed that the pequi without thorn is genetically isolated from the other populations of pequi with thorn at the endocarp, proving that this characteristic is related to the genetic divergence of the species. Analysis in BLASTn evidenced the similarity of the Dof1 genes of Zea mays to its gene of phosphinotricin acetyl transferase. In the analysis of BLASTx, the similarity was verified to the proteins responsible for the deficiency in ferric reductase 4, and catalase.

scholarly journals Genetic characterization of Italian honeybees,Apis mellifera ligustica, based on microsatellite DNA polymorphisms

Apidologie ◽  
2007 ◽  
Vol 38 (2) ◽  
pp. 207-217 ◽  
Author(s):  
Raffaele Dall'Olio ◽  
Alberto Marino ◽  
Marco Lodesani ◽  
Robin F.A Moritz
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Dna ◽  
Genetic Characterization ◽  
Dna Polymorphisms ◽  
Apis Mellifera Ligustica

scholarly journals Genetic characterization of the Moxotó goat breed using RAPD markers

2005 ◽  
Vol 40 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Rejane Rodrigues de Oliveira ◽  
Andréa Alves do Egito ◽  
Maria Norma Ribeiro ◽  
Samuel Rezende Paiva ◽  
Maria do Socorro Maués Albuquerque ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Rapd Markers ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Goat Breed ◽  
Breeding Programs ◽  
Analysis Of Molecular Variance ◽  
Polymorphic Bands

The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.

scholarly journals Randomly Amplified Polymorphic DNA Analysis in the Genus Hosta

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1243-1245 ◽  
Author(s):  
Roger J. Sauve ◽  
Suping Zhou ◽  
Yingchun Yu ◽  
Wolfram George Schmid
Keyword(s):  
Phylogenetic Relationships ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
The Other ◽  
Randomly Amplified Polymorphic Dna ◽  
Genetic Traits ◽  
Phylogenetic Clustering ◽  
Single Primer ◽  
Cluster 2

A randomly amplified polymorphic DNA (RAPD) technique was used to identify and determine the phylogenetic relationships of 37 hosta accessions representing the major subgenera, sections and groups in the genus Hosta. Results of this study show that RAPD markers were able to differentiate not only the main groups, whose plants shared many genetic traits, but also cultivars within a species. Some accessions were identified by a single primer while others had high intercross linkage and required many markers for their separation. The phylogenetic clustering showed that H. plantaginea, the only night-blooming species, and H. ventricosa, the only known natural tetraploid, are unique and should be classified separately. The four species in the subgenus Bryocles, section Lamellatae H. venusta, H. minor, H. capitata, and H. nakaiana have very low genetic similarity since they do not share many amplified fragments. The other accessions were classified into four main clusters; cluster 1: H. venusta, H. tardiva, H. pycnophylla, H. tsushimensis `Ogon', H. montana, H. tibae, H. montana f. macrophylla, H. kikutii `Kikutii', H. longissima `Longifolia', H. rectifolia `Rectifolia', H. takahashii and H.`Undulata'; cluster 2: H. laevigata, H. sieboldiana, H. pycnophylla × H. longipes f. latifolia, H. longipes `Urajiro' and H. ibukiensis; cluster 3: H. capitata, H. kikutii `Polyneuron', H. nigrescens, H. kikutii `Yakusimensis', H. pachyscapa, H. kikutii `Caput-Avis', H. longipes f. latifolia, H. hypoleuca, H. okamotoi, H. densa and H. takiensis; and cluster 4: H. aequinoctiiantha, H. rupifraga, H. `Amanuma', H. minor and H. kikutii `Densa'.

scholarly journals Genetic Similarity of Commerson’s Anchovy across Segara Anakan Cilacap Assessed Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Jurnal Biota ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Muhammad Khoerol Anam ◽  
Adi Amurwanto ◽  
Kusbiyanto Kusbiyanto ◽  
Hendro Pramono ◽  
M Husein Sastranegara ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Differences ◽  
Population Genetic Study ◽  
Randomly Amplified Polymorphic Dna ◽  
High Genetic Diversity ◽  
Impressive Result

Segara Anakan areas can be divided into three different regions according to their salinity. Salinity differences suggested that Commerson’s anchovy population in that area can be divided into three subpopulations due to genetic differences. Genetic differences among subpopulation can be assessed through a population genetic study using random amplified polymorphic DNA. This study aims to evaluate the genetic variation and differences of Commerson's anchovy (Stolephorus commersonnii) collected at three different water salinities in Segara Anakan estuary Cilacap Indonesia. Total genomic DNA was isolated using the Chelex method. Genetic diversity and differences were assessed using RAPD markers and were analyzed statistically using an analysis of molecular variance, as implemented in Arlequin software.  The results showed that high genetic diversity was observed within the subpopulations. However, no significant genetic differences were observed among subpopulations which indicate genetic similarity. A high number of offspring are likely to cause high genetic variation within subpopulations.  Adult and larvae migration is the cause of genetics similarity across Segara Anakan. Another impressive result is that water salinity did not affect the genetic characteristic of Commerson,s anchovy. Genetic similarity of Commerson’s anchovy indicates that Segara Anakan forms a single genetic conservation unit.

scholarly journals KARAKTER GENETIK POPULASI IKAN MAS, C yprinus carpio HASIL PERSILANGAN

2006 ◽  
Vol 1 (2) ◽  
pp. 227
Author(s):  
Didik Ariyanto ◽  
Estu Nugroho ◽  
Subagyo Subagyo
Keyword(s):  
Genetic Structure ◽  
Genetic Distance ◽  
Genetic Characterization ◽  
Potato Starch ◽  
Protein Electrophoresis ◽  
Fish Breeding ◽  
Starch Gel ◽  
Electrophoresis Method ◽  
Genetic Characters

Evaluasi karakter genetik populasi ikan mas hasil persilangan telah dilakukan dengan menggunakan metode elektroforesis protein secara horisontal. Sampel ikan sebanyak 120 ekor yang mewakili 6 populasi ikan mas adalah hasil persilangan resiprokal antara 3 strain ikan mas unggul yaitu strain majalaya, rajadanu dan sutisna.  Bagian tubuh yang diambil untuk pengamatan karakter genetik adalah jaringan otot sedangkan sebagai media elektroforesis digunakan gel pati.  Pada penelitian ini digunakan 12 sistem enzim untuk analisis.  Hasil analisis menunjukkan bahwa 3 lokus polimorfik dari 7 lokus yang teridentifikasi, yaitu Ldh-1, Mdh-1 dan Me. Heterozigositas rata-rata sebesar 0,23 (kategori rendah). Nilai jarak genetik paling dekat sebesar 0 diperoleh antara populasi rajadanu dengan sutisna dan nilai terjauh sebesar 0,0018 antara populasi rajadanu dan sutisna dengan majalaya. Populasi rajadanu dan sutisna mempunyai pengaruh kuat terhadap struktur genetik populasi keturunan hasil persilangan dibandingkan dengan populasi majalaya.Evaluation of genetic characterization of the hybrid populations of common carp using protein electrophoresis method was conducted.  The samples of 120 fishes were taken from Research Institute for Fish-breeding and Aquaculture Technology, Sukamandi.  The samples has been extracted from muscle and potato starch gel was used to assess the level of genetic characters.  Twelve enzymes were examined in this experiment.  The result showed that 7 loci were identified that 3 of loci were polymorphs i.e. : Ldh-1, Mdh-1 and Me.  An average of heterozigosity : 0.23 (low category). Genetic distance value between rajadanu and sutisna population was 0. Genetic distance value between both of rajadanu and sutisna populations from majalaya population was 0.0018. Rajadanu and sutisna strains have stronger influence to genetic structure of its offspring compared than majalaya strain.

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