scholarly journals Seed quality testing study in Quinoa (Chenopodium quinoa Wild.)

2020 ◽  
Vol 35 (1-2) ◽  
Author(s):  
Axay Bhuker ◽  
V. S. Mor ◽  
S. S. Jakhar ◽  
Puneeth Raj M. S.
Keyword(s):  
Seed Quality ◽  
Chenopodium Quinoa ◽  
Seed Viability ◽  
Optimum Temperature ◽  
Triphenyl Tetrazolium Chloride ◽  
Germination Test ◽  
Seedling Vigour ◽  
Tetrazolium Chloride ◽  
Quality Testing ◽  
Vigour Index

Present study was conducted on four genotypes of quinoa to find out optimum temperature and method for germination test at Department of Seed Science and Technology, CCS HAU, Hisar during 2019-20. The result of this study revealed that at 25oC temperature average germination was 22.47 per cent while it was 13.31 per cent at 20oC temperature. Similarly seedling lengths were 9.69 and 7.58cm and seedling vigour index-I were 229.54 and 103.07 at 20 and 25oC respectively. Between the methods, Top of Paper (TP) recorded 19.78 % germination while Between Paper (BP) recorded 16.00 % germination. Similarly, 8.51 and 8.77cm seedling lengths and 157.83 and 174.78 vigour index-I were observed in Top of Paper and Between Paper methods respectively. Poor germination was recorded in all four genotypes (Maximum 42%) which was confirmed by conducting tetrazolium test. It is concluded from the study that 25 oC temperature and both Top of Paper and Between Paper methods can be used for germination test in quinoa crop. For radical emergence test, 250C temperature for 60 hours should be used and for seed viability test, the seeds should soaked into 1.0% percent tetrazolium solution (2, 3, 5-triphenyl tetrazolium chloride) for 20 hours at 30oC.

scholarly journals Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.)

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger
Keyword(s):  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Growth And Yield ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.

Quantitative reduction of triphenyl tetrazolium chloride as a measure of viability in cereal seeds

1972 ◽  
Vol 12 (58) ◽  
pp. 517 ◽  
Author(s):  
RL Harty ◽  
LG Paleg ◽  
D Aspinall
Keyword(s):  
Sample Size ◽  
Standard Procedure ◽  
Seed Viability ◽  
Close Correlation ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Enzyme Systems ◽  
Dehydrogenase Enzyme ◽  
Viable Seeds ◽  
Extracting Solvent

The reduction of 2,3,5-triphenyl tetrazolium chloride (TTC) to a coloured formazan derivative by dehydrogenase enzyme systems in viable seeds has been examined. TTC reduction occurs in dry but not wetmilled tissue and the coloured product could be extracted by any of several organic solvents. This formazan product was estimated spectrophotometrically at 480 nm. The influence of fineness of grinding the tissue, TTC concentration, period of treatment, sample size, vacuum infiltration of the milled tissue with TTC and of extracting solvent were examined and a standard procedure is suggested. Using this procedure, a close correlation between seed viability and TTC reduction was demonstrated. The advantages of this method over the widely used topographical method for estimating seed viability with TTC are discussed.

scholarly journals Seed quality enhancement through biopriming in common bean (Phaseolus vulgaris. L)

2017 ◽  
Vol 9 (3) ◽  
pp. 1740-1743
Author(s):  
S. P. Monalisa ◽  
J. K. Beura ◽  
R. K. Tarai ◽  
M. Naik
Keyword(s):  
Phaseolus Vulgaris ◽  
Trichoderma Harzianum ◽  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Shoot Length ◽  
Quality Enhancement ◽  
Phaseolus Vulgaris L ◽  
Seedling Vigour ◽  
Vigour Index

An experiment on seed quality enhancement of common bean (Phaseolus vulgaris L.) var. S 9 (local) was conducted at the department of seed science and technology, OUAT, Bhubaneswar during 2013-14 by use of three biocontrol agents viz. Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescence. Seeds were bi-oprimed with the biocontrol agents at 40, 50 and 60 % concentration for 4,8,12 and 16 hours of soaking. Seeds were also hydro primed for 4,8,12 and 16 hours. Unprimed dry seed resulted in germination (69 %), shoot length (27.5 cm), root length (14 cm), seedling dry weight (1.71g), SVI-I (2859.2), SVI-II (118.0) and speed of germination (5.8) while hydro primed seeds resulted in germination (72%), shoot length (31.9 cm), root length (15 cm), seedling dry weight (1.80 g), SVI-1 (3375.9) SVI-II (129.8) and speed of germination (6.7). Trichoderma harzianum at 40% con-centration and for 4 hours of soaking resulted enhancement of above quality parameter like 13.0 % in germination, 21.1 % in shoot length, 20.7 % in root length, 31.6 % in seedling dry weight, 36 % in seedling vigour index-I, 48.1 % in seedling vigour index-II and 58.6 % in speed of germination over unprimed seeds. Bio priming with P. fluorescence ( at 40% concentration and for 4 hour) closely followed and at par with best treatment with 11.6 %, 18.2 %, 16.4 %, 30.4 %, 30.7 % and 56.9 % enhancement of above mentioned quality parameters, respectively.

scholarly journals Effect of nano based seed treatment insecticides on seed quality in Pigeonpea

2017 ◽  
Vol 9 (2) ◽  
pp. 1226-1235
Author(s):  
B.N. Raghu ◽  
Basave Gowda ◽  
S. N. Vasudevan ◽  
Sangeeta I. Macha ◽  
Sharan Gouda Hiregoudar ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Treatment ◽  
Seed Quality ◽  
Dry Weight ◽  
High Energy ◽  
Seed Kernel ◽  
Neem Seed ◽  
Seedling Vigour ◽  
Vigour Index ◽  
Sweet Flag

A laboratory experiment was conducted to know the effect seed treatment with nano insecticides on seed quality of pigeonpea (Cajanus cajan (L.) Millsp.) cv. TS3R. This study was conducted to evaluate the effect of macro and nano insecticides on seed germination and vigour of Pigeonpea. Different recommended seed treatment insecticides viz, malathion, fenvalerate, emamectine benzoate, thiodicarb, sweet flag and neem seed kernel powder insecticides were synthesized to nano form using high energy planetary ball mill. The Pigeonpea seed were treated with different nano insecticides i.e., 10-90 per cent reduction in actual dosage. Among the different treatments studied, seed treated with nano malathion 50 per cent lesser than normal dosage, fenvalerate 60 per cent lesser, thiodicarb 10 per cent lesser, emamectine benzoate 30 per cent lesser, sweetflag 70 per cent lesser, neem seed kernel powder 40 per cent lesser than actual recommended dosage gave significantly higher seed germination (98.0, 98.67, 98.67, 97.0, 99.0 and 98.67 percent) ,less number of abnormal seedlings (1.0, 0.33, 1.0, 1.0, 1.0 and 0.33 per cent) , shoot length (10.13, 9.00, 11.47, 9.50, 10.90 and 10.87 cm), root length (12.56, 12.93, 12.83, 12.60 11.50 and 13.00 cm), seedling dry weight (85.73, 87.40, 88.47, 87.70, 88.60 and 88.27 g) and seedling vigour index (2223, 2164, 2397, 2143, 2217 and 2354) as compared to untreated seeds and macro insecticides. Therefore, it is very clear that nano based insecticides has a significant (0.1 %) impact on the seed quality improvement.

scholarly journals EFFECT OF STORAGE CONTAINERS AND SEED TREATMENTS ON SEED GERMINATION AND VIGOUR OF BLACK GRAM (VIGNA MUNGO (L.) HEPPER)

2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
J.Johnny subakar ivin ◽  
Y.Anbuselvam subakar ivin ◽  
M.Surendhar J.R. Jerish ◽  
M.Surendhar J.R. Jerish
Keyword(s):  
Shelf Life ◽  
Seed Quality ◽  
Seed Viability ◽  
Seed Storage ◽  
Wire Mesh ◽  
Monthly Interval ◽  
Black Gram ◽  
Neem Oil ◽  
The Poor ◽  
Vigour Index

One of the most important basic needs for higher agricultural production is quality seed, characterized by high viability and vigour. Maintenance of seed viability and vigour from Harvest till the next growing season is of the utmost importance in a seed production programme. During seed storage, qualitative and quantitative losses of up to 20% have been reported in India. The poor seed quality may also be due to the poor storability which is very often being decided by the internal and external factors. In pulses, the major cause for seed deterioration during storage is bruchid damage. In this context, evolving an improved storage strategy to prolong the shelf life of seeds under ambient storage conditions with easily available cost-effective resources was carried out. The experiment was conducted at the Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai nagar. Freshly harvested seeds of black gram cv. ADT 3 were cleaned and graded using BSS, 8x8 wire mesh sieve. Then seeds were dried under sunlight to bring the moisture content to required level (8.5%). The seeds were treated with Thiram @ 2g kg-1, Neem oil @ 10ml kg-1 and Malathion dust @ 200 mg kg-1. Two hundred and fifty grams of seeds from all treatments along with control were packed in two containers viz. cloth bag and polythene bag (700 gauges) with three replication and stored in at ambient temperature (30o c – 35o c) and relative humidity of 52-80%. Seed samples were drawn from each replication at monthly interval for assessing the viability and vigour. Germination test was conducted in between paper method and seedlings were evaluated on seventh day for vigour index bruchid infestation. The untreated seeds recorded lower germination per cent and vigour index after seven months of storage. There was no bruchid damage on seeds treated with neem oil @ 10ml kg-1 and thiram @ 2g kg-1. It was concluded that the shelf life of black gram seeds could be increased by

scholarly journals Tetrazolium test for evaluating triticale seed viability

2010 ◽  
Vol 32 (3) ◽  
pp. 163-169 ◽  
Author(s):  
Camila Ribeiro de Souza ◽  
Osvaldo de Castro Ohlson ◽  
Melícia Ingredi Araújo Gavazza ◽  
Maristela Panobianco
Keyword(s):  
Filter Paper ◽  
Seed Viability ◽  
Test Methods ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Preconditioning Method ◽  
Ttc Test ◽  
Paper Towels ◽  
Tetrazolium Test ◽  
Glass Beaker

Freshly harvested triticale seeds are usually dormant, making the immediate evaluation of the physiological potential of seed lots difficult. We evaluated different triphenyl tetrazolium chloride (TTC) test methods for rapidly determining the viability of four seed lots of x.Triticosecale Wittmack cultivar IPR111. The test variables were: Preconditioning, (i) placing whole seeds between moistened paper towels or (ii) directly soaking the seeds in water, both procedures being conducted at 20 ºC for 18 hours; Post-conditioning seed preparation, (i) longitudinal bisection of the seed through the embryo with one half being stained and the other discarded or (ii) longitudinal bisection with both halves being stained; Staining for three and four hours, in the dark, with 0.1%, 0.5% or 1.0% (w/v) TTC according to the preconditioning method described above, (i) both halves of each seed were placed on filter paper moistened with TTC and maintained at 40 ºC or (ii) one half of each seed was immersed in 5 mL of TTC solution in a 100 mL glass beaker at 30 ºC. The best results were obtained by preconditioning seeds between moistened paper towels at 20 ºC for 18 hours and staining on filter paper with 1.0% (w/v) TTC for three hours at 40 ºC.

scholarly journals Potentiality of Bioagents on Seed Quality Enhancement in Chickpea

2014 ◽  
Vol 3 (3) ◽  
pp. 149-153 ◽  
Author(s):  
Venuturla Bharathi ◽  
Kilaru K. Durga ◽  
Madugula S. Rani
Keyword(s):  
Seed Quality ◽  
Growth Parameters ◽  
Germination Percentage ◽  
Quality Enhancement ◽  
Seedling Vigour ◽  
Vigor Index ◽  
Seedling Length ◽  
Vigour Index ◽  
Quality Character ◽  
And Storage

Planting healthy seeds and other inputs have an impact on seed quality, fungal invasion deteriorates the seed quality in soil and storage. Studies on chickpea carried out at Seed Research and Technology Center, Rajendranagar, Hyderabad during spring 2010-11 2011-12 revealed that benomyl was found effective followed by Pseudomonas fluorescens in the inhibition of mycelial growth of Fusarium solani. Maximum germination percentage was recorded with Benomyl (Benomyl 500 WP) @ 2 g/kg seed (treated check) and Tebuconazole @ 1 ml/ kg seed (95 per cent) followed by treatment with P. fluorescens @ 10 g/kg seed along with soil application of P. fluorescens @ 3 kg/acre (94 per cent). Maximization of growth parameters like root length, shoot length and total seedling length were observed with Benomyl @ 2 g/ kg seed  as 17.0 cm, 10.3 cm and 27.3 cm, respectively. Considering seedling vigour index as an important seed quality character, P. fluorescens and Benomyl @ 2 g/kg seed recorded high seedling vigor index. The per cent recovery of infested seeds was found to be low with treated seeds when compared to the control.

scholarly journals Genotype-Dependent Variation of Nutritional Quality-Related Traits in Quinoa Seeds

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2128
Author(s):  
Sara Granado-Rodríguez ◽  
Susana Vilariño-Rodríguez ◽  
Isaac Maestro-Gaitán ◽  
Javier Matías ◽  
María José Rodríguez ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
Seed Quality ◽  
Germination Rate ◽  
Genotypic Variation ◽  
Chenopodium Quinoa ◽  
Seed Viability ◽  
Plant Structure ◽  
Edible Plant ◽  
Nutritional Properties ◽  
Nutritional Characteristics

Exploiting the relationship between the nutritional properties of seeds and the genetic background constitutes an essential analysis, which contributes to broadening our knowledge regarding the control of the nutritional quality of seeds or any other edible plant structure. This is an important aspect when aiming at improving the nutritional characteristics of crops, including those of Chenopodium quinoa Willd. (quinoa), which has the potential to contribute to food security worldwide. Previous works have already described changes in the nutritional properties of quinoa seeds due to the influence of the environment, the genotype, or their interaction. However, there is an important limitation in the analyses carried out, including the outcomes that can be translated into agronomical practices and their effect on seed quality. In the present study, several seed nutritional-related parameters were analyzed in 15 quinoa cultivars grown in a particular environmental context. Important agronomical and nutritional differences were found among cultivars, such as variations in mineral or protein contents and seed viability. More importantly, our analyses revealed key correlations between seed quality-related traits in some cultivars, including those that relate yield and antioxidants or yield and the germination rate. These results highlight the importance of considering the genotypic variation in quinoa when selecting improved quinoa varieties with the best nutritional characteristics for new cultivation environments.

scholarly journals Effect of integrated nutrient management and spacing on seed quality parameters of black gram cv. Lbg-625 (rashmi)

2016 ◽  
Vol 8 (1) ◽  
pp. 340-345
Author(s):  
N. Amruta ◽  
P. J. Devaraju ◽  
Mangalagowri Mangalagowri ◽  
S. P. Kiran ◽  
H. P. Ranjitha ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seed Quality ◽  
Dry Weight ◽  
Quality Parameters ◽  
Black Gram ◽  
Seedling Vigour ◽  
Nutrient Levels ◽  
Planting Geometry ◽  
Seedling Length ◽  
Vigour Index

A field experiment was conducted to assess the response of nutrient levels and spacing on seed quality attributes of black gram cv. LBG-625 (Rashmi). Experimental results revealed that fertilizer application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- Bacillus megaterium (250 g ha-1) recorded highest test weight (39.27 g), germination (90.60%), root length (15.77 cm), shoot length (13.43 cm), mean seedling length (29.20 cm), mean seedling dry weight (57.99 mg), seedling vigour index-I (2656), seedling vigour index-II (525), total dehydrogenase activity (0.998), protein content (23.16%), field emergence (86.56 %) lowest electrical conductivity (0.813 dSm-1) were superior over other fertilizer treatments. Among the planting geometry 60 x 10 cm recorded more germination per cent (90.48 %) compared to 45 x 10 cm (87.64 %) and 30 x 10 cm (86.91 %). Interaction of nutrient levels and spacing through the application of 50:100:100 + black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm recorded more mean seedling length (34.40 cm), mean seedling dry weight (58.30 mg),and field emergence (90.24 %) lowest electrical conductivity (0.776 dSm-1) compared to control. The application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm were considered as seed quality improvement approach in blackgram, therefore conjunctive use of inorganic fertilizers and biofertilizer may be suggested for higher seed quality parameters along with overall betterment of crop.

scholarly journals Effect Effect of fruit size on physiological seed quality parameters of Cucumber (Cucumis sativus)

2019 ◽  
Vol 11 (2) ◽  
pp. 394-397
Author(s):  
Navitha P. ◽  
Sujatha K. ◽  
Beaulah A.
Keyword(s):  
Cucumis Sativus ◽  
Seed Quality ◽  
Fruit Size ◽  
Poor Quality ◽  
Quality Parameters ◽  
Seedling Vigour ◽  
Matter Production ◽  
Agricultural College ◽  
Vigour Index ◽  
Fruit Grading

An experiment was carried out at the Department of Seed Science and Technology, Agricultural College and Research Institute, Madurai during 2018 to find out the effect of fruit size on physiological seed quality of cucumber. Variation in fruit size of cucumber results in poor quality seeds. In order to overcome this obstacle fruit grading was done based on weight of fruit to obtain good quality seeds. Harvested fruits of cucumber (Cucumis sativus) were categorized based on the weight into three different groups viz., Big (2.41kg), medium (1.66kg) and small (1.28kg). Observations on fruit and seed quality parameters were recorded. The results revealed that medium sized fruits recorded higher values compared to big and small sized fruits. The number of seeds/fruit recorded higher in medium sized fruit (935 numbers) followed by small (896 numbers) and big (876 numbers) sized fruits. The big, medium and small fruits were recovered to 1.52 %, 1.06% and 0.58% seeds respectively. The physiological quality characters measured in terms of seed germination revealed that seeds of medium sized fruits were recorded higher (80%) followed by seeds of big (82%) and small (65%). The seedling vigour measured through root (17.08cm) and shoot length (14.45cm), dry matter production (0.85g 10 seedlings-1) and vigour index (2522) also proved the superiority in medium sized fruits.

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