scholarly journals Efficacy of Bio-agents in the Management of Root Knot Nematode (Meloidogyne graminicola) in Basmati Rice

Author(s):  
Abhishek Kumar ◽  
Kamal Khilari ◽  
Sachin Kumar Jain

Background: Rice root knot nematode (M. graminicola) is considered as a major threat in basmati rice. The main characteristic symptoms produced by M. graminicola are terminal hook shaped or spiral galls on the roots and other symptoms of damage include patches of stunted and yellowish plants. Methods: A field experiment was conducted in Kharif season of 2018 and 2019 at Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (UP) to test the efficacy of bio-agents against the root knot nematode. In this experiment, three bio-agents (Bacillus pumilus, Bacillus subtilis and Pseudomonas florescence) and one chemical (carbofuran) were tested in randomized block design with three replications. Result: It was observed that tested bio-agents and chemical significantly reduced the galls per plant in comparison to the control. At 30 and 60 day after transplanting, minimum (12.7 and 10.0 galls/plant) and (20.9 and 18.8 galls/plant) were recorded with Bacillus subtilis @ 20g/ M2 (2 x 108 cfu/g) during 2018 and 2019, respectively. In case of control, 52.7 and 62.5 galls/plant were recorded at 60 days after transplanting during both years. All the tested bio-agents were found effective in reducing the nematode population, increase the growth parameters and yield of basmati rice.

Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 25
Author(s):  
Toto Sunarto ◽  
Tarkus Suganda ◽  
Martua Suhunan Sianipar ◽  
Aep Wawan Irawan

ABSTRACTInduced systemic resistance in rice plant with plant extract to rice root-knot nematode (Meloidogyne graminicola Golden and Birchfiels)Rice is one of important crop that constantly infected by various pathogens. Root-knot nematodes (Meloidogyne graminicola) can decrease rice productivity in Southeast Asia. These nematodes have been reported in Indonesia. Currently nematode control is focused on biological control, application of organic and inorganic materials, natural nematicide, and induction of resistance. The study was conducted in the greenhouse Department of Plant Pest and Disease, Faculty of Agriculture, Universitas Padjadjaran. The purpose of this study was to obtain an effective type of plant extract as an inducer of rice plant resistance to M. graminicola. The experiment used experimental method with Randomized Block Design consisting of 6 treatments and 4 replications. The treatment consists of application of plant leaf extract: kirinyuh (Chromolaena odorata), beluntas (Plucea indica), water hyacinth (Eichornia crassipes), spinach thorn (Amaranthus spinosus), control (without plant extract), and carbofuran. The experimental results showed that the extract of beluntas (P. indica) and spinach thorn (A. spinosus) can decrease the amount of gall on the roots of rice plants, and can suppress the amount of juvenile II M. graminicola in 100 ml of soil.Keywords: Induced systemic resistance, Meloidogyne graminicola, Plant extract, RiceABSTRAKTanaman padi merupakan tanaman serealia penting di dunia. Patogen tanaman seperti jamur, bakteri, virus, dan nematoda merupakan faktor pembatas pada budidaya tanaman padi. Nematodabengkak akar (Meloidogyne graminicola) dapat menurunkan produktivitas padi di Asia Tenggara, dan nematoda ini telah dilaporkan terdapat di Indonesia. Pengendalian nematoda parasit tanaman sangat sulit, umumnya menggunakan nematisida kimia yang berbahaya bagi manusia dan lingkungan. Saat ini pengendalian nematoda difokuskan pada pengendalian secara biologi, aplikasi bahan organik dan inorganik, nematisida alami, dan induksi resistensi. Penelitian ini dilakukan di rumah kaca Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran, Jatinangor. Tujuan penelitian adalah untuk memperoleh jenis ekstrak tumbuhan yang efektif sebagai bahan penginduksi ketahanan tanaman padi terhadap M. graminicola. Penelitian menggunakan metode percobaan dengan Rancangan Acak Kelompok terdiri dari 6 perlakuan dan 4 ulangan. Perlakuan terdiri dari kontrol (tanpa ekstrak tumbuhan), aplikasi ekstrak daun tumbuhan kirinyuh, beluntas, eceng gondok, bayam duri, dan karbofuran. fek ekstrak tumbuhan sebagai bahan penginduksi tanaman diuji terhadap jumlah gall pada akar, jumlah juvenile tingkat kedua (J2) M. graminicola dalam tanah, berat basah bagian atas tanaman, berat basah akar, dan tinggi tanaman padi. Hasil percobaan menunjukkan bahwa aplikasi ekstrak daun tanaman bayam duri dan beluntas dengan metode seed treatment (perendaman benih padi) dan soil drench (penyiraman pada tanah sekitar tanaman padi) dapat menurunkan jumlah gall pada akar padi dan tanaman padi resisten terhadap M. graminicola. Ekstrak daun bayam duri dan beluntas dapat menekan jumlah J2 M. graminicola dalam tanah dan meningkatkan berat basah akar dan tinggi tanaman padi.Kata Kunci: Ekstrak tumbuhan, Ketahanan sistemik terinduksi, Meloidogyne graminicola, Padi


Author(s):  
Karter Nyodu ◽  
Debanand Das

Pot experiment was conducted to evaluate the efficacy of two formulations (talc formulation and vermi formulation) of four bacterial nematode biocontrol agents viz., Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonus flourescens as seed treatment against root knot nematode, Meloidogyne incognita on tomato. All the bioagents were tries at 10 and 20g / kg of seed. The experiment was terminated at 60 days after sowing the seed. The results revealed that all the treatments significantly increased the plant growth parameters and reduced the nematode multiplication over untreated control. However, maximum plant growth parameters and minimum galls per root system, eggmasses per root system, eggs per egg masses and final nematode population was recorded when seeds of tomato (var. Pusa Ruby) was treated with talc formulation of Pseudomonus flourescens @20g/kg followed by seed treatment with vermi formulation of Bacillus subtilis @ 20g/kg of seed.


Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 25
Author(s):  
Toto Sunarto ◽  
Tarkus Suganda ◽  
Martua Suhunan Sianipar ◽  
Aep Wawan Irwan

ABSTRACTInduced systemic resistance in rice plant with plant extract to rice root-knot nematode (Meloidogyne graminicola Golden and Birchfiels)Rice is one of important crop that constantly infected by various pathogens. Root-knot nematodes (Meloidogyne graminicola) can decrease rice productivity in Southeast Asia. These nematodes have been reported in Indonesia. Currently nematode control is focused on biological control, application of organic and inorganic materials, natural nematicide, and induction of resistance. The study was conducted in the greenhouse Department of Plant Pest and Disease, Faculty of Agriculture, Universitas Padjadjaran. The purpose of this study was to obtain an effective type of plant extract as an inducer of rice plant resistance to M. graminicola. The experiment used experimental method with Randomized Block Design consisting of 6 treatments and 4 replications. The treatment consists of application of plant leaf extract: kirinyuh (Chromolaena odorata), beluntas (Plucea indica), water hyacinth (Eichornia crassipes), spinach thorn (Amaranthus spinosus), control (without plant extract), and carbofuran. The experimental results showed that the extract of beluntas (P. indica) and spinach thorn (A. spinosus) can decrease the amount of gall on the roots of rice plants, and can suppress the amount of juvenile II M. graminicola in 100 ml of soil.Keywords: Induced systemic resistance, Meloidogyne graminicola, Plant extract, RiceABSTRAKTanaman padi merupakan tanaman serealia penting di dunia. Patogen tanaman seperti jamur, bakteri, virus, dan nematoda merupakan faktor pembatas pada budidaya tanaman padi. Nematoda bengkak akar (Meloidogyne graminicola) dapat menurunkan produktivitas padi di Asia Tenggara, dan nematoda ini telah dilaporkan terdapat di Indonesia. Pengendalian nematoda parasit tanaman sangat sulit, umumnya menggunakan nematisida kimia yang berbahaya bagi manusia dan lingkungan. Saat ini pengendalian nematoda difokuskan pada pengendalian secara biologi, aplikasi bahan organik dan inorganik, nematisida alami, dan induksi resistensi. Penelitian ini dilakukan di rumah kaca Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran, Jatinangor. Tujuan penelitian adalah untuk memperoleh jenis ekstrak tumbuhan yang efektif sebagai bahan penginduksi ketahanan tanaman padi terhadap M. graminicola. Penelitian menggunakan metode percobaan dengan Rancangan Acak Kelompok terdiri dari 6 perlakuan dan 4 ulangan. Perlakuan terdiri dari kontrol (tanpa ekstrak tumbuhan), aplikasi ekstrak daun tumbuhan kirinyuh, beluntas, eceng gondok, bayam duri, dan karbofuran. fek ekstrak tumbuhan sebagai bahan penginduksi tanaman diuji terhadap jumlah gall pada akar, jumlah juvenile tingkat kedua (J2) M. graminicola dalam tanah, berat basah bagian atas tanaman, berat basah akar, dan tinggi tanaman padi. Hasil percobaan menunjukkan bahwa aplikasi ekstrak daun tanaman bayam duri dan beluntas dengan metode seed treatment (perendaman benih padi) dan soil drench (penyiraman pada tanah sekitar tanaman padi) dapat menurunkan jumlah gall pada akar padi dan tanaman padi resisten terhadap M. graminicola. Ekstrak daun bayam duri dan beluntas dapat menekan jumlah J2 M. graminicola dalam tanah dan meningkatkan berat basah akar dan tinggi tanaman padi.Kata Kunci: Ekstrak tumbuhan, Ketahanan sistemik terinduksi, Meloidogyne graminicola, Padi


2020 ◽  
Vol 57 (4) ◽  
pp. 302-309
Author(s):  
Sukanta Pal ◽  
Megha Sana ◽  
Hirak Banerjee ◽  
Lhingneikim Lhungdim

Effect of nitrogen and bio-fertilizer on growth and productivity of hybrid rice (cv. PHB 71) was assessed during dry seasons of 2017 and 2018 at Research Farm of BCKV under new alluvial zone of West Bengal. The experiment was laid out in factorial randomized block design with 12 treatment combinations having 4 levels of N and 3 types of bio-fertilizer replicated thrice. Application at 180 kg N/ha produced tallest plants at 90 DAT with maximum LAI (60 DAT), DMA (90 DAT), tillers/hill (90 DAT) and CGR (30-60 & 60-90 DAT). The same N rate resulted in highest panicles/m2, panicle length, panicle weight, filled grains/panicle and 1000-grain weight. A decrease in N dose from 180 to 150 kg/ha caused reduction in all those yield components; however, the variation was non-significant except for number of panicles/m2. Maximum grain yield, straw yield and harvest index was also achieved with 180 kg N/ha accounting 120.1, 34.9 and 32.8% more than the values obtained with zero-N; however, it was statistically at par with the yields and HI obtained with 150 kg N/ha. The Azospirillum application increased all the growth parameters, yield components and yield of hybrid rice over other tested bio-fertilizers (Azospirillum > PSB > K mobilizer), accounting 5.9 and 8.8% more than the yields obtained with PSB and K mobilizer. The interaction of N and bio-fertilizer exerted significant effect on growth attributes but failed to record any significant variations in yield components and yield of hybrid rice. The maximum economic benefit was achieved with combined application of 180 kg N/ha and Azospirillum.


2020 ◽  
Vol 7 (01) ◽  
Author(s):  
SK SRIVASTAVA ◽  
PAWAN JEET

A study was conducted to assess the effect of drip irrigation and plastic mulch on growth and seed yield of Semialata. Two types of plastic mulch (green and silver/black) were tested at three levels of irrigation (120%, 100% and 80%) by drip irrigation and one level (100%) by furrow irrigation. The daily water requirement of Semialata was estimated by the equation ETcrop= ETox crop factor. ETcrop is crop water requirement mm/day. ETo (reference evapotranspiration, mm/day) was calculated by FAO calculator which uses temperature and humidity data. In this experiments there were twelve treatments were considered. The treatments were replicated thrice. The experiment was laid in randomized block design. It was observed that drip irrigation with or without plastic mulch is yielding better results in terms of growth parameters and seed yield as compared to furrow irrigation without plastic mulch. It was also observed that maximum suppression (67.58%) of weeds resulted with drip irrigation and silver/black plastic mulch at 80% level of irrigation.


1970 ◽  
Vol 9 ◽  
pp. 21-27 ◽  
Author(s):  
Nabin Kumar Dangal ◽  
D. Sharma Poudyal ◽  
S. M. Shrestha ◽  
C. Adhikari ◽  
J. M. Duxbury ◽  
...  

Pot experiment was conducted during July-September 2006 to evaluate some organic amendments such as sesame (Sesamum indicum) biomass, buckwheat (Fagopyrum esculentum) biomass, neem (Azadirachta indica) leaves, chinaberry (Melia azedarch) leaves and chicken manure @ 1, 2 and 3 t ha-1 each against the rice root-knot nematode (Meloidogyne graminicola Golden & Birchfield) in direct seeded rice. The treatments were replicated five times in a randomized complete block design. The number of second stage juveniles (J2) of M. graminicola was significantly low in chicken manure @ 3 t ha-1. The root knot severity index was significantly low in sesame @ 3 t ha-1, chinaberry @ 3, 2 or 1 t ha-1, neem @ 3 t ha-1 and chicken manure @ 2 or 3 t ha-1 amended soil but root lesion severity index was lower only in chicken manure @ 2 t ha-1 treated plots. The fresh shoot weight and length were significantly high in chicken manure amendment @ 2 or 3 t ha-1 at 45th day after seeding. However, the fresh root weight, length, number of leaves and number of J2 recovered from the roots were non-significant. Key words: biomass; juveniles; Meloidogyne graminicola; root-knot severity index; root lesion severity index DOI: 10.3126/njst.v9i0.3160 Nepal Journal of Science and Technology 9 (2008) 21-27


2017 ◽  
Vol 47 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Marília Barcelos Souza Lopes ◽  
Taynar Coelho de Oliveira Tavares ◽  
Danilo Alves Veloso ◽  
Niléia Cristina da Silva ◽  
Rodrigo Ribeiro Fidelis

ABSTRACT The population increase and the need of intensifying food production, coupled with the scarcity of water resources, have led to the search of alternatives that reduce consumption and optimize the water use during cultivation. In this context, hydrogels become a strategy in agricultural management, due to their water retention capacity in the soil and availability to plants. This study aimed at evaluating the efficiency of hydrogels on the development and production of cowpea bean ('Sempre-verde' cultivar) under water stress, in a greenhouse. The experiment was performed in a randomized block design, with five replications, in a 4 x 5 factorial scheme, consisting of four types of hydrogel (Hydroplan-EB HyA, with granulometry of 1-3 mm; Hydroplan-EB HyB, with granulometry of 0.5-1 mm; Hydroplan-EB HyC, with granulometry < 0.5 mm; Polim-Agri, with granulometry of 1-0.5 mm) and five concentrations (0 g pot-1; 1.5 g pot-1; 3 g pot-1; 4.5 g pot-1; 6 g pot-1). The following traits were evaluated: number of pods per plant, number of grains per pod and grain yield. The highest concentration (6 g pot-1) resulted in a higher number of pods and yield for all the hydrogels, especially for HyC and Polim-Agro, which presented 7.4 pods plant-1 and 7.0 pods plant-1, with yield of 15.43 g plant-1 and 16.68 g plant-1, respectively. The use of hydrogel shows to be efficient for reducing yield losses under water stress.


Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 8
Author(s):  
Hersanti Hersanti ◽  
Sudarjat Sudarjat ◽  
Andina Damayanti

ABSTRACTThe ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotchPurple blotch disease caused by Alternaria porri is one of the major disease on shallot. One of the methods that can be applied to control the disease is the use of antagonistic bacteria. Antagonistic bacteria can be used as a resistance inducer to suppress pathogen development. In this study, Bacillus subtilis and Lysinibacillus sp. were formulated in carbon fiber as a carrier and nano silica 3% as a complementary. This study was conducted to determine the ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotch. The experiment was conducted at the Laboratory of Phytopathology, Departement of Plant Pest and Diseases and Ciparanje Green House, Faculty of Agriculture, Universitas Padjadjaran from December 2017 until July 2018. Suspension of Bacillus subtilis and Lysinibacillus sp. singly or mixed were formulated in carbon fiber 80 Mesh and 3% nano silica. The experiment used Randomized Block Design consisted of 8 treatments with 3 replications. Each replication consisted of 5 plants. The results showed that the mixture of Bacillus subtilis and Lysinibacillus sp. in 3% silica nano and carbon fiber was the ablest treatment to increase the resistance of shallot to purple blotch by 71,2%.Keywords: Antagonistic bacteria, BiocontrolABSTRAKPenyakit bercak ungu yang disebabkan oleh Alternaria porri merupakan salah satu penyakit utama pada bawang merah. Salah satu metode yang dapat digunakan untuk pengendalian yaitu menggunakan bakteri antagonis. Bakteri antagonis dapat digunakan sebagai penginduksi ketahanantanaman untuk menekan perkembangan penyakit. Pada penelitian ini, Bacillus subtilis dan Lysinibacillus sp. diformulasikan dalam serat karbon sebagai bahan pembawa dan silika nano 3% sebagai bahan pelengkap. Percobaan ini dilakukan untuk mengetahui kemampuan bakteri Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran dalam serat karbon dan silika nano3% untuk menginduksi ketahanan bawang merah terhadap penyakit bercak ungu. Percobaan ini dilaksanakan di Laboratorium Fitopatologi, Departemen Hama dan Penyakit Tumbuhan dan Rumah Kaca, Ciparanje, Fakultas Pertanian, Universitas Padjadjaran mulai dari bulan Desember 2017 hingga Juli 2018. Suspensi Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran diformulasikan pada serat karbon dan silika nano 3%. Percobaan menggunakan rancangan acak kelompok yang terdiri dari 8 perlakuan dengan 3 ulangan. Setiap ulangan terdiri dari 5 tanaman. Dari hasil percobaan diketahui bahwa campuran B. subtilis dan Lysinibacillus sp. dalam silika nano 3% serta serat karbon mampu meningkatkan ketahanan bawang merah terhadap penyakit bercak ungu dengan persentase hambatan sebesar 71,2%.Kata Kunci: Bakteri antagonis, Biokontrol


Author(s):  
Hari Kesh ◽  
Khushi Ram Battan ◽  
Rakesh Kumar

Background: Basmati rice is an important cereal crop occupying a unique position in Indian agriculture. More than 90% of global rice is produced and consumed in Asia and plays a crucial role in the entry of mineral nutrients into the food chain. Identification of stable genotypes is of great significance because the environmental conditions vary from season to season and year to year. Methods: Thirty six Basmati rice genotypes were evaluated in four production environments during kharif 2016 and kharif 2017 at two locations Kaul and Uchani to study the G × E interaction for milling, appearance, cooking and eating quality parameters. The genotypes were grown in randomized block design with three replications. Result: Based on the stability analysis of Eberhart and Russell model, genotypes viz., Haryana Mahak 1, Pusa 1826-12-271-4 and HKR 06-434 were found stable across the environments for milling%, grain length before cooking and length breadth ratio before cooking, respectively.


2019 ◽  
Vol 11 (9) ◽  
pp. 83
Author(s):  
Jackson de M. Alves ◽  
Alex S. de Lima ◽  
Cesenildo de F. Suassuna ◽  
Francisco R. A. Figueiredo ◽  
Toshik Iarley da Silva ◽  
...  

Zucchini (Cucurbita pepo L.) is a horticultural crop of great socioeconomic importance in Brazil and in the world. However, inappropriate fertilization management, such as over-fertilization of soils, may become a limiting factor for its development. Thus, the aim of this study was to evaluate the morpho-physiological behavior of zucchini submitted to nitrogen (N) doses applied via soil and foliar application of silicon (Si). The treatments were distributed in split-plot scheme in a randomized block design, with three replications. The plot was formed by silicon levels (0.0 and 6.0 g plant-1) and the subplots constituted by five nitrogen levels (30, 60, 90, 120 and 150 kg ha-1), adding up to 30 experimental units. Gas exchanges and growth parameters were assessed at 35 days after planting. The data were submitted to analysis of variance by the F test and in the cases of significance was performed a polynomial regression analysis for the nitrogen factor and Tukey test for the silicon factor. The supply of Si positively enhances the effects of N on growth characteristics and gas exchanges of zucchini. The simultaneous application of Si and N does not influence the leaf area of zucchini. The N dose of 93.9 kg ha-1 provides greater assimilation of CO2 in zucchini plants under the conditions in which the experiment was performed.


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