scholarly journals ​Effect of Ferula Elaeochytris Root Extract on Smooth Muscle Contraction of Prostate Gland in Rat

2021 ◽  
Author(s):  
Ozge Ozturk Cimentepe ◽  
Cemil Gocmen ◽  
Nadire Eser ◽  
Hacer Sinem Buyuknacar ◽  
Mehmet Cimentepe
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Prostate Gland ◽  
Electrical Field Stimulation ◽  
Smooth Muscle Contraction ◽  
Prostate Tissue ◽  
Male Rats ◽  
Root Extract ◽  
Isolated Rat

Background: Smooth muscle contraction and enlargement of the prostate are important targets for the treatment of lower urinary tract symptoms in patients with benign prostatic hyperplasia. The effect of Ferula elaeochytris (FE) root extract on tissues that play a role on fertility such as the prostate has not been demonstrated yet. The aim of this study is to investigate the effects of FE extract on isolated rat prostate tissue induced by electrical field stimulation (EFS) in vitro. Methods: In this experimental study, 48 male rats were randomly divided into 6 groups (n = 8). Groups were consisting FE (20 µl), FE + Adenosine triphosphate (ATP; 50 μM), FE + prazosin (0.3 μM), FE + Calcium (Ca2+; 3 mM and 6 mM), FE + suramin (100 μM), FE + phenylephrine and FE + carbachol. Result: In our study, FE extract inhibited the neurogenic contractions induced by EFS on isolated rat anterior prostate tissue. The presence of suramin and prazosin were not significantly increase the inhibition caused by the FE extract, while Ca2+ and ATP significantly increased the inhibition by the FE extract. (p less than 0.05). In addition, FE extract significantly inhibited phenylephrine and carbachol contractions (p less than 0.05).

Amikacin: A Novel Modulator of Vesical and Prostate Efferences. An in vitro Experimental Study

2007 ◽  
Vol 74 (4) ◽  
pp. 217-227
Author(s):  
M. Gardi ◽  
F. Nigro ◽  
E. Ragazzi ◽  
A. Volpe ◽  
A. Totaro ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Tract ◽  
Electrical Field Stimulation ◽  
Smooth Muscle Contraction ◽  
Detrusor Muscle ◽  
Detrusor Smooth Muscle ◽  
Related Compounds ◽  
Human Detrusor ◽  
Isolated Rat

The autonomic efferent neurotransmission to the bladder and prostate smooth muscle is a potential target for drug therapy of specific low urinary tract disfunction (LUTD). Since amikacin and other amynoglicosides were reported to affect neurotransmission by a pre-junctional mechanism, we investigated the effect of amikacin on isolated rat and human detrusor smooth muscle contraction and on isolated rat and human prostate contraction, to further evaluate its potential relaxant properties. Materials and Methods Samples of detrusor smooth muscle and prostate tissue, obtained from 97 rats and 16 patients undergoing surgery, were studied through the measurement of isometric contraction induced by electrical field stimulation (EFS) and other pharmacological stimuli in the presence or absence of 1mM amikacin in a low-Ca medium. Results Amikacin 1 mM significantly reduced contraction of isolated rat and human detrusor muscle and prostate, achieved with pre-junctional stimulation, while no significant effect was observed on contraction induced by pharmacological post-junctional stimulators. EFS contraction inhibited by amikacin was restored after addition of calcium chloride. The amikacin effect was comparable to the effect of magnesium ions, which are known to exert a pre-junctional inhibition of neurotransmitter release. Conclusions Amikacin significantly inhibited rat and human detrusor and prostate contraction evoked by pre-junctional stimulation in vitro, suggesting a depressant effect on autonomic efferent neurotransmission. Further pharmacokinetics studies and researches on related compounds may hold potential for future development in the treatment of specific low urinary tract disfunction (LUTD).

scholarly journals RELAXATION EFFECT OF ETHANOLIC EXTRACT OF AVERRHOA BILIMBI L. LEAVES ON ILEUM SMOOTH MUSCLE CONTRACTION OF IN VITRO ISOLATED RAT (RATTUS NORVEGICUS)

2018 ◽  
Vol 11 (13) ◽  
pp. 135 ◽  
Author(s):  
Rulia Meilina, ◽  
Edy Suwarso ◽  
Edy Suwarso ◽  
Edy Suwarso ◽  
Aminah Dalimunthe ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Rattus Norvegicus ◽  
Ethanolic Extract ◽  
Relaxation Effect ◽  
Smooth Muscle Contraction ◽  
Ileum Contraction ◽  
Isolated Rat

Objective: The study was aimed to investigate the relaxation effect of ethanolic extract of Averrhoa bilimbi L. leaves (EEABL) and ondansetron against 5-HT-induced contraction of the 5-HT3 receptor.Methods: The study of the relaxation effect of the cumulative concentration EEABL leaves (0.5–4 mg/mL) and ondansetron (10−5–3×10−2) after the contracted with 5-HT (EC80: 1.158×10−6 M) was conducted in vitro using isolated rat’s ileum organ in the Tyrode solution.Results: The EEABL and ondansetron decreased ileum contraction after induced by 5- HT. EEABL has no difference in terms of ability as ondansetron in reducing the ileum smooth muscle contraction induced by 5-HT (p>0.05).Conclusion: The EEABL has relaxation effect on smooth muscle of rat’s isolated ileum which has induced by 5-HT.

Defect in colonic smooth muscle contraction in patients with ulcerative colitis

1991 ◽  
Vol 261 (6) ◽  
pp. G987-G991 ◽  
Author(s):  
W. J. Snape ◽  
R. Williams ◽  
P. E. Hyman
Keyword(s):  
Ulcerative Colitis ◽  
Smooth Muscle ◽  
Muscle Contraction ◽  
Diverticular Disease ◽  
Electrical Field Stimulation ◽  
Smooth Muscle Contraction ◽  
Inhibitory Neurotransmitter ◽  
Patients With Cancer ◽  
Low Amplitude

Patients with ulcerative colitis have decreased postprandial colonic contractions. The purpose of this study was to determine whether the smooth muscle from patients with ulcerative colitis responds abnormally in vitro to different stimuli. Circular colonic smooth muscle strips from patients with ulcerative colitis, acute diverticular disease, or adenocarcinoma were stretched to the optimal length and stimulated with electrical field stimulation (EFS), bethanechol, or increased concentrations of extracellular K+. The EFS-stimulated on-contraction was similar in each group, but the off-contraction was decreased in patients with colitis compared with patients with cancer (P less than 0.02) or diverticular disease (P less than 0.01). Bethanechol stimulated a dose-dependent colonic contraction, which was less in the strips from patients with colitis compared with cancer (P less than 0.02) or diverticular disease (P less than 0.05). The response to increased extracellular K+ was less in muscle from patients with colitis (P less than 0.01) than in the other tissues. Muscle from diverticular disease developed greater stress to K+ stimulation than did muscle from cancer (P less than 0.05). These studies suggest that there is a decrease in the force of muscle contraction in colonic muscle obtained from patients with colitis compared with normal muscle resected from patients with cancer or with muscle associated with diverticular disease of the colon. The similar relatively low amplitude of the on-contraction in each group suggests the physiological release of an inhibitory neurotransmitter.

scholarly journals Effects of Aqueous Extract of Curcuma Longa Rhizome On Motility of Isolated Rabbit Jejunum

2019 ◽  
Vol 2 (1) ◽  
pp. 13-22
Author(s):  
B Umaru
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Aqueous Extract ◽  
Curcuma Longa ◽  
Muscle Relaxation ◽  
Soxhlet Extraction ◽  
Smooth Muscle Contraction ◽  
Smooth Muscle Relaxation ◽  
Rabbit Jejunum

Turmeric (curcuma longa) is a rhizomatous herbaceous perennial plant of the ginger family and the order Zingerberales. It is widely cultivated and used in the treatment of various ailments. In this study, the effect of aqueous extract of C. longa on isolated rabbit jejunum was investigated in vitro using Physiograph (Meditech, India). The rhizome of Curcumin was extracted using Soxhlet extraction method and distilled water was used as a solvent. The elemental analysis was determined using AAS and the result revealed the presence of Potassium, Magnesium, Iron and Nitrogen. The percentage concentrations of trace elements in the aqueous Curcumin rhizome were within the WHO standard limit. The aqueous extract at concentration tested (100 mg/ml) significantly decreased (p<0.05) jejunum smooth muscle contraction. Addition of Atropine (1mM) or Propranolol (1mM) further decreased the amplitude of jejunum smooth muscle contraction. Curcumin rhizome (100 mg/ml) blocked contraction induced by Ach (0.001μg/ml). The result of this work has shown that rhizome of C. longa produced jejunum smooth muscle relaxation, plant extract with antispasmodic activity may reduce gastrointestinal motility thereby delay gastric emptying and may be important in treatment of disease ailments like diarrhoea and colic.

Role of platelets in contraction of canine tracheal muscle elicited by PAF in vitro

1988 ◽  
Vol 65 (2) ◽  
pp. 914-920 ◽  
Author(s):  
K. J. Popovich ◽  
G. Sheldon ◽  
M. Mack ◽  
N. M. Munoz ◽  
P. Denberg ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Airway Epithelium ◽  
Platelet Activating Factor ◽  
Smooth Muscle Contraction ◽  
Tracheal Smooth Muscle ◽  
Serotonin Antagonist

To elucidate mechanisms of platelet-activating factor (PAF)-induced contraction, we studied the effect of PAF on 203 canine tracheal smooth muscle (TSM) strips from 45 dogs in vitro in the presence and absence of platelets. PAF (10(-11) to 10(-7) M) alone caused no contraction of TSM even in the presence of airway epithelium. In the presence of 2 x 10(5) platelets/microliter, PAF was an extremely potent contractile agonist (threshold 10(-11) M). This response was inhibited by the PAF antagonist, CV-3988 (10(-6) M), and reversed by the serotonin antagonist, methysergide (EC50 = 3.7 +/- 0.79 x 10(-9) M). Neither atropine nor chlorpheniramine (10(-9) to 10(-6) M) attenuated the response to PAF + platelets. In the presence of platelets, 10(-7) M PAF caused an increase in perfusate concentration of serotonin from 0.93 +/- 0.037 x 10(-8) to 1.7 +/- 0.046 x 10(-8) M (P less than 0.001). Tachyphylaxis, previously demonstrated to be irreversible, was shown to be a platelet-dependent phenomenon; contraction could be repeated in the same TSM after addition of fresh platelets. We demonstrate that PAF-induced contraction of canine TSM is caused by the release of cellular intermediates such as serotonin from platelets. We also demonstrate the site of PAF-induced tachyphylaxis in airway smooth muscle contraction.

Insulin acutely increases agonist-induced airway smooth muscle contraction in human and rat

2021 ◽  
Author(s):  
Becky J. Proskocil ◽  
Gina N. Calco ◽  
Zhenying Nie
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Contraction ◽  
Intracellular Calcium ◽  
Mrna Expression ◽  
Airway Smooth Muscle ◽  
Muscle Cells ◽  
Smooth Muscle Contraction ◽  
Rat Tracheal Smooth Muscle ◽  
Isolated Rat

Obesity increases incidence and severity of asthma but the molecular mechanisms are not completely understood. Hyperinsulinemia potentiates vagally induced bronchoconstriction in obese rats. Since bronchoconstriction results from airway smooth muscle contraction, we tested whether insulin changed agonist-induced airway smooth muscle contraction. Obesity prone and resistant rats were fed a low-fat diet for 5 weeks and treated with insulin (Lantus, 3 units/rat s.c.) 16 h before vagally induced bronchoconstriction was measured. Ex vivo, contractile responses to methacholine were measured in isolated rat tracheal rings and human airway smooth muscle strips before and after incubation (0.5 - 2 h) with 100 nM insulin or 13.1 nM insulin like growth factor-1 (IGF-1). M2 and M3 muscarinic receptor mRNA expression was quantified by qRT-PCR and changes in intracellular calcium were measured in response to methacholine or serotonin in isolated rat tracheal smooth muscle cells treated with 1 µM insulin. Insulin, administered to animals 16 h prior, potentiated vagally induced bronchoconstriction in both obese prone and resistant rats. Insulin, not IGF-1, significantly increased methacholine-induced contraction of rat and human isolated airway smooth muscle. In cultured rat tracheal smooth muscle cells, insulin significantly increased M2, not M3, mRNA expression and enhanced methacholine- and serotonin-induced increase in intracellular calcium. Insulin alone did not cause an immediate increase in intracellular calcium. Thus, insulin, acutely potentiated agonist-induced increase in intracellular calcium and airway smooth muscle contraction. These findings may explain why obese individuals with hyperinsulinemia are prone to airway hyperreactivity and give insights into future targets for asthma treatment.

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