scholarly journals Comparative analysis of gene expression in tea plant (Camellia sinensis (L.) Kuntze) under low-temperature stress

2020 ◽  
Vol 24 (6) ◽  
pp. 598-604
Author(s):  
L. S. Samarina ◽  
A. O. Matskiv ◽  
N. G. Koninskaya ◽  
T. A. Simonyan ◽  
V. I. Malyarovskaya ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Comparative Analysis ◽  
Low Temperature ◽  
Temperature Stress ◽  
Leaf Tissue ◽  
Frost Tolerance ◽  
Tea Plant ◽  
Low Temperature Stress ◽  
Germplasm Collections ◽  
Significant Difference

Low-temperature stress is one of the main factors limiting the distribution and reducing the yield of many subtropical crops, including the tea crop. Efficient breeding to develop frost-tolerant cultivars requires a reliable set of genetic markers for identifying resistance donors, and that is why it is necessary to reveal the specific genetic response in frost-tolerant genotypes in comparison with frost- susceptible ones. In this work, we performed a comparative analysis of the expression of 18 tea genes (ICE1, CBF1, DHN1, DHN2, DHN3, NAC17, NAC26, NAC30, bHLH7, bHLH43, P5CS, WRKY2, LOX1, LOX6, LOX7, SnRK1.1, SnRK1.2, SnRK1.3) under cold and frost conditions in two tea genotypes, tolerant and susceptible. Low-temperature stress was induced by placing the potted plants in cold chambers and lowering the temperature to 0…+2 °С for 7 days (cold stress), followed by a decrease in temperature to –4…–6 °С for 5 days (frost stress). Relative electrical conductivity of leaf was measured in response to the stress treatments, and a significant difference in the frost tolerance of the two tea genotypes was confirmed. Cold exposure did not lead to a change in the electrical conductivity of leaf tissue. On the other hand, frost treatment resulted in increased REC in both genotypes and to a greater extent in the susceptible genotype. Increased expression of all the genes was shown during cold and frost. The genes that were strongly expressed in the tolerant tea genotype were revealed: ICE1, CBF1, DHN2, NAC17, NAC26, bHLH43, WRKY2, P5CS, LOX6, SnRK1.1, SnRK1.3. These genes can be proposed as markers for the selection of frost-tolerance donors in tea germplasm collections. Additionally, it was shown that the tolerant genotype is characterized by an earlier response to stress at the stage of cold acclimation. The study of the expression of the identified genes in different organs of tea plants and in different exposures to low temperature is relevant for further investigations.

scholarly journals Physiological Attributes and Transcriptomics Analysis Reveal the Response Mechanism of Helictotrichon Virescens to Low-Temperature Stress

2021 ◽  
Author(s):  
Mingjun Cheng ◽  
Mingmin Zheng ◽  
Kuoshu Cui ◽  
Ruizhen Zhang ◽  
Tao Yang ◽  
...  
Keyword(s):  
Low Temperature ◽  
Cold Stress ◽  
Temperature Stress ◽  
Leaf Tissue ◽  
Low Temperature Stress ◽  
Tibet Plateau ◽  
Perennial Herb ◽  
Optical Antenna ◽  
Response Mechanism ◽  
Antenna Complex

Abstract Background: Helictotrichon Virescens is a perennial herb mainly distributed in high altitude areas of 2000 ~ 4500 m. It is widely cultivated in the Qinghai-Tibet Plateau of China, strongly resistant to cold, and an essential part of wild herbage in this region. However, the molecular mechanism of Helictotrichon virescens response to low-temperature stress is unclear, and the key regulating genes to specific biological processes are poorly known. Results: It was used physiological and transcriptome analysis to study the cold stress response mechanism in Helictotrichon Virescens. Under low-temperature treatment at 0℃, the leaves reduced chlorophyll content and light energy absorption through light antenna complex (LHCs). As the content of chlorophyll, a and b decreased, as evident were the photoprotection effects. Besides, circadian pathway-plant is crucial for the response to cold stress in Helictotrichon Virescens. Among them, DEG encoding LHY and HY5 showed strongly up-regulated expression during cold stress in Helictotrichon Virescens. Conclusions: This study provides an opportunity to fully understand the response process of Helictotrichon Virescens to low-temperature stress. It answers the pertinent questions in perennial herbaceous cold stress research, how leaves integrate light and low-temperature signals to regulate circadian rhythms in crops, and how leaf tissue reduces light absorption and enhances light protection through optical antenna complex (LHCs).

Effects of low-temperature stress on histone modification in ZF4 cells

2019 ◽  
Vol 26 (2) ◽  
pp. 280
Author(s):  
Penglei JIANG ◽  
Yingdi SHI ◽  
Yanwen HOU ◽  
Bingshe HAN ◽  
Junfang ZHANG
Keyword(s):  
Low Temperature ◽  
Histone Modification ◽  
Temperature Stress ◽  
Low Temperature Stress

Effects of low temperature stress on photosynthesis in potato leaves

2014 ◽  
Vol 39 (1) ◽  
pp. 26-30 ◽  
Author(s):  
Yu-zhi QIN ◽  
Jue CHEN ◽  
Zhen XING ◽  
Chang-zheng HE ◽  
Xing-yao XIONG
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Low Temperature Stress

scholarly journals Zinc Seed Priming Improves Spinach Germination at Low Temperature

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 271
Author(s):  
Muhammad Imran ◽  
Asim Mahmood ◽  
Günter Neumann ◽  
Birte Boelt
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Multispectral Imaging ◽  
Germination Rate ◽  
Cost Effective ◽  
Seed Priming ◽  
Seedling Development ◽  
Low Temperature Stress ◽  
Early Seedling ◽  
Early Seedling Development

Low temperature during germination hinders germination speed and early seedling development. Zn seed priming is a useful and cost-effective tool to improve germination rate and resistance to low temperature stress during germination and early seedling development. Spinach was tested to improve germination and seedling development with Zn seed priming under low temperature stress conditions. Zn priming increased seed Zn concentration up to 48 times. The multispectral imaging technique with VideometerLab was used as a non-destructive method to differentiate unprimed, water- and Zn-primed spinach seeds successfully. Localization of Zn in the seeds was studied using the 1,5-diphenyl thiocarbazone (DTZ) dying technique. Active translocation of primed Zn in the roots of young seedlings was detected with laser confocal microscopy. Zn priming of spinach seeds at 6 mM Zn showed a significant increase in germination rate and total germination under low temperature at 8 °C.

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