scholarly journals Achievements and prospects of applying high-throughput sequencing techniques to potato genetics and breeding

2017 ◽  
Vol 21 (1) ◽  
pp. 96-103 ◽  
Author(s):  
I. V. Bykova ◽  
N. A. Shmakov ◽  
D. A. Afonnikov ◽  
A. V. Kochetov ◽  
E. K. Khlestkina
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Potato Genetics

Achievements and prospects of applying high-throughput sequencing techniques to potato genetics and breeding

2017 ◽  
Vol 7 (7) ◽  
pp. 736-743 ◽  
Author(s):  
I. V. Bykova ◽  
N. A. Shmakov ◽  
D. A. Afonnikov ◽  
A. V. Kochetov ◽  
E. K. Khlestkina
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Potato Genetics

DETECTION OF ALFACORONAVIRUSES, BETACORONAVIRUSES AND ASTROVIRUSES IN BAT FECAL SAMPLES FROM MOSCOW REGION

2020 ◽  
Author(s):  
E.V. Korneenko ◽  
◽  
А.E. Samoilov ◽  
I.V. Artyushin ◽  
M.V. Safonova ◽  
...  
Keyword(s):  
High Throughput ◽  
Viral Genome ◽  
High Throughput Sequencing ◽  
Pcr Amplification ◽  
Moscow Region ◽  
Fecal Samples ◽  
Genus Level ◽  
Zoonotic Viruses ◽  
Reservoir Hosts ◽  
Blastn Search

In our study we analyzed viral RNA in bat fecal samples from Moscow region (Zvenigorod district) collected in 2015. To detect various virus families and genera in bat fecal samples we used PCR amplification of viral genome fragments, followed by high-throughput sequencing. Blastn search of unassembled reads revealed the presence of viruses from families Astroviridae, Coronaviridae and Herpesviridae. Assembly using SPAdes 3.14 yields contigs of length 460–530 b.p. which correspond to genome fragments of Coronaviridae and Astroviridae. The taxonomy of coronaviruses has been determined to the genus level. We also showed that one bat can be a reservoir of several virus genuses. Thus, the bats in the Moscow region were confirmed as reservoir hosts for potentially zoonotic viruses.

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