scholarly journals A comparative study of replicative properties of antitumor recombinant vaccinia viruses on human glioblastoma cell culture U87 and monkey kidney cell culture CV-1

2016 ◽  
Vol 20 (6) ◽  
pp. 949-953
Author(s):  
R. A. Maksyutov ◽  
I. V. Kolosova ◽  
T. V. Tregubchak ◽  
I. A. Razumov ◽  
S. N. Shchelkunov
Keyword(s):  
Cell Culture ◽  
Comparative Study ◽  
Kidney Cell ◽  
Monkey Kidney ◽  
Glioblastoma Cell ◽  
Monkey Kidney Cell ◽  
Recombinant Vaccinia ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell ◽  
Vaccinia Viruses

scholarly journals A novel 3D human glioblastoma cell culture system for modeling drug and radiation responses

2016 ◽  
pp. now164 ◽  
Author(s):  
Natividad Gomez-Roman ◽  
Katrina Stevenson ◽  
Lesley Gilmour ◽  
Graham Hamilton ◽  
Anthony J Chalmers
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Glioblastoma Cell ◽  
Cell Culture System ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell

Characterization and fibrin-binding properties of different molecular forms of pro-urokinase from a monkey kidney cell culture

1986 ◽  
Vol 42 (6) ◽  
pp. 749-760 ◽  
Author(s):  
G. Wijngaards ◽  
D.C. Rijken ◽  
A.L. van Wezel ◽  
E. Groeneveld ◽  
C.A.M. Van der Velden
Keyword(s):  
Cell Culture ◽  
Kidney Cell ◽  
Monkey Kidney ◽  
Molecular Forms ◽  
Monkey Kidney Cell ◽  
Binding Properties ◽  
Kidney Cell Culture

Characteristics of a Mycoplasma hyorhinis strain contaminating a human fetal diploid cell line

1969 ◽  
Vol 15 (5) ◽  
pp. 451-454 ◽  
Author(s):  
J. Joncas ◽  
A. Chagnon ◽  
G. Lussier ◽  
V. Pavilanis
Keyword(s):  
Cell Culture ◽  
Kidney Cell ◽  
Cytopathic Effect ◽  
Fetal Lung ◽  
Monkey Kidney ◽  
Direct Immunofluorescence ◽  
Monkey Kidney Cell ◽  
Acridine Orange Staining ◽  
Kidney Cell Culture ◽  
Accessory Factor

A human fetal lung cell culture contaminant possessing most of the characteristics of that described by Girardi et al. (5) was shown to belong to the M. hyorhinis-GDL group of cytopathic mycoplasma by accessory factor neutralization test. The homotypic antiserum in this test neutralized the contaminant as well as three closely related strains while it could inhibit the growth of the contaminant only on agar. The mycoplasma could be detected in tissue cultures by the acridine orange staining technique and by direct immunofluorescence. These appeared as minute coccobacillary bodies either isolated or in clumps, predominantly extracellular in the original tissue culture but apparently intracellular in monkey kidney cell culture. A titer of 106 TCID50 per 0.1 ml was obtained in monkey kidney cell culture and the cytopathic effect induced in this culture was inhibited by pretreatment of the agent with 20% ether for 18 hours at 4 C, with tris buffer at pH 3 for 3 hours, or by heating at 56 C for 3 minutes. The cytopathic effect was suppressed by chlortetracycline (50 μg/ml) and neutralized with accessory factor by specific antiserum produced in rabbit up to a titer of 8192, whereas a variety of normal human and animal sera failed to do so. The source of the contaminant could not be determined.

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