scholarly journals Adaptation of the brain tumors classifier, constructed on the direct mass spectrometric profiling data, to MALDI mass spectrometry analysis

2018 ◽  
pp. 24-24
Keyword(s):  
Mass Spectrometry ◽  
Brain Tumors ◽  
Maldi Mass Spectrometry ◽  
Mass Spectrometric ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
The Brain

Direct Automated MALDI Mass Spectrometry Analysis of Cellular Transporter Function: Inhibition of OATP2B1 Uptake by 294 Drugs

2020 ◽  
Vol 92 (17) ◽  
pp. 11851-11859
Author(s):  
Melissa S. Unger ◽  
Lena Schumacher ◽  
Thomas Enzlein ◽  
David Weigt ◽  
Maciej J. Zamek-Gliszczynski ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Maldi Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis

scholarly journals Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

2012 ◽  
Vol 8 ◽  
pp. 753-762 ◽  
Author(s):  
Martin J Weissenborn ◽  
Johannes W Wehner ◽  
Christopher J Gray ◽  
Robert Šardzík ◽  
Claire E Eyers ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometric ◽  
Mass Spectrometry Analysis ◽  
Spectrometric Analysis ◽  
Spectrometry Analysis ◽  
Maldi Tof ◽  
Glass Slides ◽  
Array Format ◽  
Tof Ms

Glycans functionalised with hydrophobic trityl groups were synthesised and adsorbed onto polystyrene and glass slides in an array format. The adsorbed glycans could be analysed directly on these minimally conducting surfaces by MALDI-TOF mass spectrometry analysis after aluminium tape was attached to the underside of the slides. Furthermore, the trityl group appeared to act as an internal matrix and no additional matrix was necessary for the MS analysis. Thus, trityl groups can be used as simple hydrophobic, noncovalently linked anchors for ligands on surfaces and at the same time facilitate the in situ mass spectrometric analysis of such ligands.

Hybrid biomaterials: Surface-MALDI mass spectrometry analysis of covalent binding versus physisorption of proteins

2000 ◽  
Vol 17 (1) ◽  
pp. 23-35 ◽  
Author(s):  
Keith M. McLean ◽  
Sally L. McArthur ◽  
Ronald C. Chatelier ◽  
Peter Kingshott ◽  
Hans J. Griesser
Keyword(s):  
Mass Spectrometry ◽  
Covalent Binding ◽  
Maldi Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Hybrid Biomaterials

Mass Spectrometry-Based Proteomic Approach in Juglans regia

2021 ◽  
Vol 885 ◽  
pp. 25-31
Author(s):  
Donatella Aiello
Keyword(s):  
Mass Spectrometry ◽  
Juglans Regia ◽  
Maldi Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Intact Protein ◽  
Protein Fractionation ◽  
Spectrometry Analysis ◽  
Protein Mass ◽  
Proteomic Approach ◽  
Mass Information

We proposed a MALDI mass spectrometry based approach to characterize walnut allergens. The strategy is based on the extraction of hydro-soluble tissue proteins followed by protein fractionation and mass-spectrometry analysis. Linear MALDI was adopted to evaluate the intact protein mass information and the presence of glycoproteins.

Application of Mass Spectrometry Methods to the Analysis of Lipid Composition of the Human Brain

2021 ◽  
Vol 37 (5) ◽  
pp. 80-87
Author(s):  
A.I. Tkachev ◽  
M.S. Osetrova ◽  
D.N. Smirnov ◽  
O.I. Efimova ◽  
E.E. Khrameev
Keyword(s):  
Mass Spectrometry ◽  
Human Brain ◽  
Lipid Composition ◽  
Large Scale ◽  
Lipid Extraction ◽  
Simultaneous Detection ◽  
Molecular Organization ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
The Brain

Abstract-Lipids make up more than half of the dry matter of the human brain and play a key role in its functioning. However, the lipid composition of the brain anatomical structures remains poorly understood. The first such studies were carried out more than 50 years ago, but since then, a small number of works have been published describing the concentration of lipids in only a few areas of the human brain. A fundamentally new step towards understanding the molecular organization of the brain and identifying the molecular basis of human cognitive abilities should be a detailed large-scale study of the brain lipidome. However, there is no description in the literature of methods optimized for studying the lipid composition of the human brain. In this work, we develop and present methods for lipid extraction and mass spectrometry analysis, which ensure simultaneous detection of the maximum amount of different lipid classes and individual substances in the human brain, as well as the approaches to bioinformatics analysis of the obtained data. Their use makes it possible to create a comprehensive picture of the molecular organization of the human brain, which has no analogues in the world in terms of its completeness. Key words: lipidome, brain, mass spectrometry, high-performance liquid chromatography, bioinformatics The reported study was funded by the Russian Foundation for Basic Research, project no. 20-34-70077.

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