scholarly journals Fluid shear stress induces epithelial-mesenchymal transition (EMT) in Hep-2 cells

Oncotarget ◽  
2016 ◽  
Vol 7 (22) ◽  
pp. 32876-32892 ◽  
Author(s):  
Shuangfeng Liu ◽  
Fating Zhou ◽  
Yang Shen ◽  
Yingying Zhang ◽  
Hongmei Yin ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Epithelial Mesenchymal Transition ◽  
Fluid Shear ◽  
Mesenchymal Transition

scholarly journals Fluid shear stress coupled with narrow constrictions induce cell type-dependent morphological and molecular changes in circulating tumor cells

2019 ◽  
Author(s):  
Hamizah Ahmad Cognart ◽  
Jean-Louis Viovy ◽  
Catherine Villard
Keyword(s):  
Shear Stress ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Fluid Shear Stress ◽  
Dynamical Behavior ◽  
Metastatic Breast ◽  
Microfluidic Channels ◽  
Mechanical Stimuli ◽  
Fluid Shear ◽  
Mesenchymal Transition

AbstractCancer mortality mainly arises from metastases, due to cells that escape from a primary tumor, circulate in the blood as circulating tumor cells (CTCs), permeate across blood vessels and nest in distant organs. It is still unclear how CTCs overcome the harsh conditions of fluid shear stress and mechanical constraints within the microcirculation. Here, a model of the blood microcirculation was established through the fabrication of microfluidic channels comprising constrictions. Metastatic breast cancer cells of epithelial-like and mesenchymal-like phenotypes were flowed into the microfluidic device. These cells were visualized during circulation, analyzed for their dynamical behavior and retrieved post-circulation. γ-H2AX staining showed significant increase of DNA damage response in epithelial-like SK-BR-3 cells, while gene expression analysis of key regulators of epithelial-to-mesenchymal transition revealed significant increase of Twist2 relative expression in mesenchymal-like MDA-MB-231 cells post-circulation. This work documents first results of the changes at the cellular, subcellular and molecular scales induced by the two main mechanical stimuli arising from circulatory conditions.

scholarly journals Endothelial-to-mesenchymal transition contributes to fibro-proliferative vascular disease and is modulated by fluid shear stress

2015 ◽  
Vol 108 (3) ◽  
pp. 377-386 ◽  
Author(s):  
Jan-Renier A.J. Moonen ◽  
Ee Soo Lee ◽  
Marc Schmidt ◽  
Monika Maleszewska ◽  
Jasper A. Koerts ◽  
...  
Keyword(s):  
Shear Stress ◽  
Vascular Disease ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Mesenchymal Transition ◽  
Endothelial To Mesenchymal Transition ◽  
Proliferative Vascular Disease

scholarly journals Epigenetic Changes During Mechanically Induced Osteogenic Lineage Commitment

2015 ◽  
Vol 137 (2) ◽  
Author(s):  
Julia C. Chen ◽  
Mardonn Chua ◽  
Raymond B. Bellon ◽  
Christopher R. Jacobs
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Shear Stress ◽  
Fluid Shear Stress ◽  
Methylation Status ◽  
Lineage Commitment ◽  
Fluid Shear ◽  
Osteogenic Lineage ◽  
Osteogenic Markers ◽  
Heterochromatin Structure

Osteogenic lineage commitment is often evaluated by analyzing gene expression. However, many genes are transiently expressed during differentiation. The availability of genes for expression is influenced by epigenetic state, which affects the heterochromatin structure. DNA methylation, a form of epigenetic regulation, is stable and heritable. Therefore, analyzing methylation status may be less temporally dependent and more informative for evaluating lineage commitment. Here we analyzed the effect of mechanical stimulation on osteogenic differentiation by applying fluid shear stress for 24 hr to osteocytes and then applying the osteocyte-conditioned medium (CM) to progenitor cells. We analyzed gene expression and changes in DNA methylation after 24 hr of exposure to the CM using quantitative real-time polymerase chain reaction and bisulfite sequencing. With fluid shear stress stimulation, methylation decreased for both adipogenic and osteogenic markers, which typically increases availability of genes for expression. After only 24 hr of exposure to CM, we also observed increases in expression of later osteogenic markers that are typically observed to increase after seven days or more with biochemical induction. However, we observed a decrease or no change in early osteogenic markers and decreases in adipogenic gene expression. Treatment of a demethylating agent produced an increase in all genes. The results indicate that fluid shear stress stimulation rapidly promotes the availability of genes for expression, but also specifically increases gene expression of later osteogenic markers.

Patterns of fluid shear stress determine atherosclerotic lesion size and vulnerability

2006 ◽  
Vol 45 (3) ◽  
pp. e51
Author(s):  
Caroline Cheng ◽  
Dennie Tempel ◽  
Luc van Damme ◽  
Rien van Haperen ◽  
Rob Krams ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Atherosclerotic Lesion ◽  
Lesion Size ◽  
Fluid Shear

The rate of fluid shear stress is a potent regulator for the differentiation of mesenchymal stem cells

2019 ◽  
Vol 234 (9) ◽  
pp. 16312-16319 ◽  
Author(s):  
Danyang Yue ◽  
Mengxue Zhang ◽  
Juan Lu ◽  
Jin Zhou ◽  
Yuying Bai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Shear Stress ◽  
Mesenchymal Stem Cells ◽  
Fluid Shear Stress ◽  
Fluid Shear
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