scholarly journals Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow

Oncotarget ◽  
2016 ◽  
Vol 7 (10) ◽  
pp. 10788-10802 ◽  
Author(s):  
Gabrielis Kundrotas ◽  
Evelina Gasperskaja ◽  
Grazina Slapsyte ◽  
Zivile Gudleviciene ◽  
Jan Krasko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Genomic Stability ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Proliferation Capacity ◽  
Low Volume

scholarly journals Cytological Effects of Serum Isolated from Polytraumatized Patients on Human Bone Marrow-Derived Mesenchymal Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-18
Author(s):  
Yazhou Long ◽  
Katrin Bundkirchen ◽  
Pascal Gräff ◽  
Christian Krettek ◽  
Sandra Noack ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Damage Control ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Damage Control Orthopedics ◽  
Proliferation Capacity ◽  
Healthy Donors

Due to their immunomodulatory and regenerative capacity, human bone marrow-derived mesenchymal stem cells (hBMSCs) are promising in the treatment of patients suffering from polytrauma. However, few studies look at the effects of sera from polytraumatized patients on hBMSCs. The aim of this study was to explore changes in hBMSC properties in response to serum from polytrauma patients taken at different time points after the trauma incident. For this, sera from 84 patients with polytrauma (collected between 2010 and 2020 in our department) were used. In order to test the differential influence on hBMSC, sera from the 1st (D1), 5th (D5), and 10th day (D10) after polytrauma were pooled, respectively. As a control, sera from three healthy donors (HS), matched with respect to age and gender to the polytrauma group, were collected. Furthermore, hBMSCs from four healthy donors were used in the experiments. The pooled sera of HS, D1, D5, and D10 were analyzed by multicytokine array for pro-/anti-inflammatory cytokines. Furthermore, the influence of the different sera on hBMSCs with respect to cell proliferation, colony forming unit-fibroblast (CFU-F) assay, cell viability, cytotoxicity, cell migration, and osteogenic and chondrogenic differentiation was analyzed. The results showed that D5 serum significantly reduced hBMSC cell proliferation capacity compared with HS and increased the proportion of dead cells compared with D1. However, the frequency of CFU-F was not reduced in polytrauma groups compared with HS, as well as the other parameters. The serological effect of polytrauma on hBMSCs was related to the time after trauma. It is disadvantageous to use BMSCs in polytraumatized patients at least until the fifth day after polytrauma as obvious cytological changes could be found at that time point. However, it is promising to use hBMSCs to treat polytrauma after five days, combined with the concept of “Damage Control Orthopedics” (DCO).

Candidate kinases for adipogenesis and osteoblastogenesis from human bone marrow mesenchymal stem cells

2021 ◽  
Author(s):  
Xia Yi ◽  
Ping Wu ◽  
Jianyun Liu ◽  
Shan He ◽  
Ying Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factors ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cytoplasmic Proteins ◽  
Marrow Mesenchymal Stem Cells

Adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis) are closely related processes involving with the phosphorylation of numerous cytoplasmic proteins and key transcription factors.

Inner ear progenitor cells can be generated in vitro from human bone marrow mesenchymal stem cells

2012 ◽  
Vol 7 (6) ◽  
pp. 757-767 ◽  
Author(s):  
Sarah L Boddy ◽  
Wei Chen ◽  
Ricardo Romero-Guevara ◽  
Lucksy Kottam ◽  
Illaria Bellantuono ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields

2012 ◽  
Vol 28 (5) ◽  
pp. 1329-1335 ◽  
Author(s):  
Hyunjin Cho ◽  
Young-Kwon Seo ◽  
Hee-Hoon Yoon ◽  
Soo-Chan Kim ◽  
Sung-Min Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Electromagnetic Fields ◽  
Low Frequency ◽  
Neural Stimulation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Extremely Low Frequency
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