scholarly journals Oxidation of heat shock protein 60 and protein disulfide isomerase activates ERK and migration of human hepatocellular carcinoma HepG2

Oncotarget ◽  
2016 ◽  
Vol 7 (10) ◽  
pp. 11067-11082 ◽  
Author(s):  
Chung-Yi Lin ◽  
Chi-Tan Hu ◽  
Chuan-Chu Cheng ◽  
Ming-Che Lee ◽  
Siou-Mei Pan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Disulfide Isomerase ◽  
Human Hepatocellular Carcinoma ◽  
Heat Shock Protein 60 ◽  
Disulfide Isomerase ◽  
And Migration ◽  
Protein Disulfide

34 DIFFERENTIALLY EXPRESSED PROTEINS OF EARLY-STAGE PLACENTA DERIVED FROM CLONED CAT EMBRYOS USING PROTEOMICS ANALYSIS

2012 ◽  
Vol 24 (1) ◽  
pp. 129
Author(s):  
J. I. Bang ◽  
D. W. Bae ◽  
Y. S. Kwon ◽  
G. K. Deb ◽  
B. H. Choi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Early Pregnancy ◽  
Protein Disulfide Isomerase ◽  
Triosephosphate Isomerase ◽  
Early Stage ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Disulfide Isomerase ◽  
Protein Disulfide

We have previously demonstrated that differentially expressed proteins affect abnormal development and function of cloned term placenta. This is associated with cloned fetus morbidity and mortality. We also frequently observed loss of the cloned fetus and failed development during early pregnancy periods. To confirm the pattern of important gene expression in cloned placenta during pre- and post-implantation, we investigated expression pattern of proteins in early stage (21 days) domestic cat placentas of cloned embryo transfer (CEP; n = 2) and artificial insemination (CP; n = 4) derived pregnancy. The differentially expressed proteins were investigated by 2-DE and MALDI-TOF/MS. Twenty-three proteins were up- and down-regulated at least 1.5-fold in the CEP (P < 0.05) compared with the CP. Differentially expressed proteins were analysed using PDQest program and statistically analysed by 1-way ANOVA using the SPSS software. In CEP, 13 proteins were up-regulated, such as 78-kDa glucose-regulated protein (GRP78), annexin A2 (ANXA2), protein DJ-1 (DJ1), adenylate kinase isoenzyme 1 (AK1), protein disulfide-isomerase A3 (PDIA3), heat shock protein β-1 (HSPB1), actin, cytoplasmic 1 (ACTB), serum albumin (ALB), protein disulfide-isomerase A6 (PDIA6), G protein-regulated inducer of neurite outgrowth 1 (GRIN1) and triosephosphate isomerase (TIM). In contrast, 10 proteins were down-regulated, such as vinculin (VCL), triosephosphate isomerase (TIM), heterogeneous nuclear ribonucleoprotein H (hnRNPH), tropomyosin α-4 (TPM4), 60-kDa heat shock protein, mitochondrial (Hsp60), serum albumin (ALB), calumenin (CALU), keratin type 1 (CK1) and prohibitin (PHB). To validate the identified proteins in the CEP compared with the CP, we investigate a peptide sequences using MALDI-TOF/TOF tandem mass spectrometry. The sequence information obtained a high ions score from NCBI and Swiss-Prot databases. In conclusion, we did identify abnormal expression of proteins that might be associated with impaired development of CEP, which may endanger the cloned fetus during early pregnancy. This work was partly supported by the BK21 program and the KOSEF (10525010001-05N2501-00110) and the Next-generation BioGreen21 program (No. PJ007990012011).

scholarly journals A novel prognostic factor for hepatocellular carcinoma: protein disulfide isomerase

2014 ◽  
Vol 29 (5) ◽  
pp. 580 ◽  
Author(s):  
Su Jong Yu ◽  
Jae-Kyung Won ◽  
Han Suk Ryu ◽  
Won-Mook Choi ◽  
Hyeki Cho ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Prognostic Factor ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Protein Disulfide

Protein disulfide isomerase inhibition synergistically enhances the efficacy of sorafenib for hepatocellular carcinoma

Hepatology ◽  
2017 ◽  
Vol 66 (3) ◽  
pp. 855-868 ◽  
Author(s):  
Jae-Kyung Won ◽  
Su Jong Yu ◽  
Chae Young Hwang ◽  
Sung-Hwan Cho ◽  
Sang-Min Park ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Protein Disulfide

Correlation between Clinicopathology and Immunolocalization of Heat Shock Protein 72 and Glycoprotein 96 in Human Hepatocellular Carcinomas

2011 ◽  
Vol 282-283 ◽  
pp. 3-6
Author(s):  
Xiao Ping Wang ◽  
Huan Ping Lin ◽  
Qiao Xia Wang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Lymph Node ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Human Hepatocellular Carcinoma ◽  
Tumor Infiltration ◽  
Heat Shock Protein 72 ◽  
Gastrointestinal Carcinomas ◽  
Hepatocellular Carcinomas ◽  
Cancer Tissues

Heat shock protein 72 (HSP72) and glycoprotein 96 (gp96) are highly expressed in cancer tissues. Recent studies indicate the possible roles of HSP72 and gp96 in the development and progression of gastrointestinal carcinomas but detailed information is still ambiguous. The aim of the study is to investigate the correlation between clinicopathology and immunolocalization of HSP72 and gp96 in human hepatocellular carcinoma. Immunohistochemistry demonstrated that HSP72 and gp96 expression in hepatocellular carcinomas with metastasis was significantly higher than those with non-metastasis. HSP72 and gp96 expression were significantly associated with the presence of tumor infiltration, lymph node and remote metastasis.

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